Histological Techniques

1. A very Warm Welcome to the
Department of Anatomy
Dr RPGMC Tanda
Dr Inderamohan Bisht

2. Histology Background & Basics
 Study of Cellular Organisation
 Greek Histos means Web/ Tissue. Study of Tissues
 Tissue coined by Bichat in 1792. Father of Histology
Sir Marie Francois Xavier Bichat(1771-1792)
 Coining of term Histology by Mayer in1819.
 Important in the understanding of many subjects of
Medical Science.
 All basic life functions by Unicellular Organism.
 Division of Labor in Multicellular Organisms.
 Group of Cells similar in Structure & Function is
Tissue.

3. Histological
Techniques
 Preparation of the
Tissues for Microscopic
Examination
 Aim is to preserve the
Microscopic Anatomy of
the Tissue.
 Tissue is made
somewhat hard so that
very thin sections4to 5
Microns can be made.
 Good Staining is
possible.
 Final stained Section
closely represents a
Live Tissue
Protocol Followed
Receipt, Identification,Labelling
Fixation
Decalcification
Dehydration
Clearing
Impregnation
Embedding
Section Cutting
Mounting on Slide
Clearing
Rehydration
Staining
Dehydration
Clearing
Mounting of Cover Slip

4. Receipt, Identification & Labelling

33. Components
of Microscope
1. Eye Piece(Ocular
Lens)
2. Revolving Nose
Piece(Hold multiple
Objective Lenses)
3. Objective Lens
4. Coarse Adjustment
5. Fine Adjustment
6. Stage(To hold the
Specimen Slide)
7. Light Source
8. Diaphragm &
Condenser
9. Mechanical Stage

34. H & E is a charge-based, general purpose stain. Hematoxylin stains acidic molecules
shades of blue. Eosin stains basic materials shades of red, pink and orange. H & E
stains are universally used for routine histological examination of tissue sections.
Hematoxylin and Eosin (H & E)

35. Units of Measurement
Study of Histology involves units
of Measurement
• 1mm = 1000Micrometer/
Micron
• 1 Micron = 1000 Nanometer
• 1 Angstrom = 0.1 Nanometer

36. Thanks a lot . A very Good, Studious & Peaceful Day to all of
You from Dr Inderamohan Bisht. May God Bless You all.
The SPIRIT within us has the ability to grow beyond all time, space &
physical matter in response
to our receptivity. Our job is to remain Open; so that we continue to
GROW