Realizado por: Verónica Gómez León

1. PRESENTADO POR: VERÓNICA GÓMEZ LEÓN
TERCER SEMESTRE DE MEDICINA.
UNIVERSIDAD PONTIFICIA BOLIVARIANA

2. INTRODUCTION Small size effects
easy-to-immobilize
targeting molecules and
controllable release
Drug delivery and
tumour imaging
Can achieve the aims of
site-directed release and
reduced toxic side effects.
Nanomedicine:
Is the comprehensive monitoring,
control, construction, repair,
defense, and improvement of
human biological systems at the
molecular level.
Using engineered nanodevices and
nanostructures
Operating massively in parallel at
the single-cell level, performing
“single-cell medicine,” ultimately to
achieve medical benefit

3. An initiating signal leads to the
formation of a specialized
membrane in association with
specific cargo.
This membrane expands, curls and
seals itself off, enclosing the cargo
and forming a double-layered
autophagosome.
which then fuses with a lysosome
leading to the degradation and
ultimate recycling of all internal
contents.
1
2
3
4
Cargo may include, glycogen granules, ribosomes, organelles
Autolysosome

4. OBJECTIVES
THIS INVESTIGATION
APPROACH TO STUDY
“SMART AND DUAL-
TARGETED BSA
NANOMEDICINE WITH
CONTROLLABLE RELEASE BY
HIGH AUTOLYSOSOME
LEVELS”

5. CULTIVO CELULAR
Detección de la tasa de supervivencia
celular utilizando métodos de MTT
METODOS
¿Para qué?
las células pueden diferenciarse en un
cultivo, y las células cultivadas responden
al tratamiento con fármacos, hormonas,
factores de crecimiento y otras sustancias
activas.
Fundamento:
Desarrollar medios definidos libres de
suero, con todos los nutrientes y
antimicrobianos necesarios que
mantengan el crecimiento de las células.
3-(4,5-dimethyl-2-thiazolyl)-2,5-
diphenyl-2-H-tetrazolium
bromide (MTT)
¿Para qué?
Para comparar los efectos el
DOX libre y la annomedicina
preparada.

6. METODOS
¿PARA QUÉ?
para poder visualizar los lugares donde
se produzca la reacción
¿PARA QUÉ?
Empleo de doble marcaje de
fluorescencia para la vigilancia de
fenómenos dinámicos dentro de los
organelos celulares de células vivas.
Clasificación de células activadas
por fluorescencia (FACS)
Tinción inmunohistoquímica
FUNDAMENTO:
Esta última permite observar la localización
de determinados compuestos que absorben
radiación ultravioleta invisible y emiten
porciones de la energía en las longitudes de
onda visibles, más largas
FUNDAMENTO:
Las técnicas inmunohistoquímicas
utilizan anticuerpos como reactivos
específicos para demostrar muchas
sustancias diferentes..

7. RESULTADOS
Endocytosis of the nanomedicine in Beas2B and PC9 cells was
observed using fluorescence microscopy (red fluorescence is DOX, blue
fluorescence is DAPI). Scale bar 10 μm.
Autophagy of PC9
cells treated with
free DOX or the
nanomedicine, 7
(A)
western blotting,
(B) confocal
fluorescence
microscope images.
Scale bar 10 μm.

8. RESULTADOS
(A) Cleaved caspase-3 and Ki-67 immunohistochemical images of PC9 tumours after treatment with free DOX or DOX-loaded nanomedicine. Scale bar 50 μm. (B) Statistical analyses of the
positive cell percentage for caspase-3 and Ki-67 staining. Bars represent the mean ± SD (n=6), * P < 0.05, ** P < 0.01. (C) Near-infrared (NIR) fluorescence in vivo image of PBS 2 h after the
tail vein injection of free DOX and BSA nanomedicine (lex. 675 ± 10 nm, lem. 694 ± 20 nm).

9. DISCUSION
AUTHORS WHAT THEY SAID THE INVESTIGATION
J. Wang et al “the free drug enters the
cytosol directly through the cell
membrane and then traffics into the
nucleus, while the nanoparticle-loaded
drug mostly traffics into endosomes
and lysosomes upon intracellular
uptake and reaches the nucleus only
after acid-triggered drug release in
endolysosomes”.
S. Zhang et al “BSA nanomedicine also enters PC9
cells via endocytosis”.
H. Kettiger et al “In this regard, endocytosis seemed
more efficient for carrying a large
amount of DOX compared to simple
diffusion through the cell membrane”

10. CONCLUSIONS
The study of cells
at the molecular
level allows us to
identify different
risk factors for
living organisms
and develop
preventive
methods.
The study of cancer
cells at the molecular
level allows us to
identify their
functioning and
reproduction, in order
to develop an effective
treatment with fewer
risks for the patient.

11. MAPA CONCEPTUAL