Preliminary Presentation
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The document describes a project to create a bio-inspired tissue phantom of the whisker barrel cortex of a rodent using microfluidics. The goals are to construct a polydimethylsiloxane microfluidic device that mimics the brain vasculature and uses programmable pumps to simulate blood flow and oxygen levels, modeling the whisker barrel cortex. Methods considered for fabricating the microfluidic device mold include 3D printing, photolithography and printed circuit boards.
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Preliminary Presentation
- 1. BOLD Simulation Device Group C Ian Barreto, Theodore Ha, Jonathan Ma, Konomi Nakajima PI: Dr. Hyle Park
- 3. Whisker Barrel Cortex (Top) : Scheme of Whisker Barrel Cortex System (Bottom): Pattern of Blood Vessels and Capillaries in whisker Barrel Cortex of a Rodent http://www2.psy.unsw.edu.au/Users/earabzadeh/Research.html
- 4. Goal Blood Vessel Skull Brain Blood Vessel
- 5. Goal Blood Vessel Skull Brain Blood Vessel
- 6. Project Overview Diagram of our Project System
- 8. Whisker Barrel Cortex Design Parameters
- 10. Print and Peel http://news.harvard.edu/gazette/wp-content/uploads/2011/01SEAS_Photo-11.jpg
- 13. Negative Molds: Photolithography General Procedure for Photolithography http://www.hitequest.com/Kiss/photolithography.gif
- 22. Budget
Editor's Notes
- When there ’s a neural activity in a certain area, the neurons in that area take in more oxygen from blood And this change in blood flow and oxygenation is what BOLD measures
- One to one spatial correspondence on the contralateral face of the rodent to large barrel like structures in the cortex As you stimulate one whisker on the rodent, the barrel region in the rat brain associated with that whisker will produce neural activity in the form of hemoglobin changes as oxygen (oxy-hemoglobin) is being comsumed by the neurons and an increase in blood flow rate as the rodent tries to resupply the area of the brain with more oxygen. Moreover the flow of blood is not constant but pulsetile and proportional to the rodent ’s heartrate. The Park research group would like to image one particular portion of the cortex while stimulating the corresponding portion of the whiskers to image these BOLD changes However, they need a controllable phantom to test their imaging system on first. And this is what we will be constructing«
- Two syringe pumps will be utilized to deliver oxygenated and deoxygenated hemoglobin to a microfluidic device that mimics the parameters of a rat whisker barrel cortex. The rates of these syringes pumps will be controlled utilizing a computer to maintain correct hemoglobin ratios. For this project, we will be creating a series of microfluidic devices with varying chamber heights ranging from larger sizes (that are easier to optically measure) down to physiologically relevant sizes.
- To begin a microfluidic device mimicking the properties of the whisker barrel cortex of a rodent is need to check the feasibility of OCT as a method to characterize BOLD. Microfluidic devices have wide applications to a number of different fields, including small-scale analytical systems, biomedical devices, and tools used by biochemists. An example is microfluidic vessels can be utilized to model the size and flow environment found in capillaries. A material that shows promise for biological and water-based applications in microfluidics is poly(dimethylsiloxane) (PDMS) because it is nontoxic and thus can be implanted in vivo . When combined with Titanium oxide or Silicon oxide to change its optical properties, the correct scattering coefficient can be obtained a value similar to biological tissue
- Limitation is height resolution of the printer: 10-20 microns 50 - 100
- viable only in early bulk stages of our design project since the z-positioning resolution was insufficient for our design parameters
- Accurate in height of the channel
- Created designs using Tanner EDA L-edit Pro software for photomask printing we designed this design We already sent it out grayphics for fabrication and we expect it to arrive this week
- Advantage is wide use and low cost. Enable us to find a collaborator on campus.
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- (a) X grams of TiO 2 are weighed on a scale (b) Polydimethylsiloxane (PDMS) and (c) curing agent were added to a plate at a ratio of 10:1. (d) X grams of TiO 2 was added (e) The two were mixed thoroughly and (f) placed in a vacuum to eliminate the bubbles from the mixture.