This document discusses the instrumentation and applications of high performance liquid chromatography (HPLC). It describes the key components of an HPLC system including the solvent reservoir, pumps, sample injection system, columns, detectors, and recorders. It provides details on the types of pumps (constant flow rate and constant pressure), sample injection loops, columns and fittings. It also explains common detectors such as UV-visible and refractive index detectors. Finally, it lists several applications of HPLC in quality control testing, analysis of biological fluids, stability studies, drug metabolism studies, and industrial uses.
Ultra high performance liquid chromatography (UHPLC) provides faster, more sensitive and higher resolution separations compared to traditional high performance liquid chromatography (HPLC). UHPLC uses columns packed with smaller particles less than 2um in diameter which allows for higher pressures and flow rates. This leads to significantly shorter run times, lower detection limits, and better resolution of peaks. The key components of a UHPLC system include pumps that can handle higher pressures, injection systems with low dwell volumes, specialized columns, and detectors capable of measuring small changes. UHPLC has applications in areas like pharmaceutical analysis, metabolomics, and impurity profiling where high resolution and sensitivity are important.
The presentation contains basic introduction to mostly used and versatile reversed phase chromatography, its instrumentation, working and applications. It will be useful for you to understand basic concepts about RP-HPLC.
High Performance Liquid Chromatography (HPLC) is presented. HPLC is a chromatographic technique used to separate mixtures by using high pressure to force a liquid mobile phase and sample through a column packed with solid stationary phase. Key aspects summarized include:
1. HPLC provides simultaneous analysis, high resolution, sensitivity, repeatability for qualitative and quantitative analysis.
2. It works on principles of adsorption and partition chromatography depending on the stationary phase.
3. Instrumentation includes pumps, injector, analytical column, detector, and recorder/integrator.
4. Parameters like retention time, capacity factor, separation factor, and plate height provide information about sample separation and column efficiency.
High performance liquid chromatography (HPLC) is a widely used technique to separate mixtures of organic compounds. HPLC works by partitioning components of a mixture between a stationary phase made of small particles in a steel column and a mobile phase solvent. Pressure is required to force the mobile phase through the stationary phase due to the small particle size. HPLC provides enhanced separations in shorter time periods compared to traditional liquid chromatography. A variety of stationary and mobile phases can be used to optimize separations of different molecules.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)Suneal Saini
This document provides an introduction to high performance liquid chromatography (HPLC). It discusses the basic components and principles of HPLC, including the stationary and mobile phases, pumps to move the mobile phase through the column at high pressure, and various detectors used to analyze the separated components as they elute from the column. It also describes the different types of HPLC based on the mode of separation, elution technique, scale of operation, and type of analysis performed.
This document provides a detailed overview of the basic components and functioning of an HPLC system. It describes the main components including the mobile phase reservoir, pump, injector, analytical column, detectors, and sample preparation. It explains the working of various types of pumps, columns, detectors and provides the Beer-Lambert law equation. The document is intended to provide a comprehensive schematic and explanation of an HPLC system and its components.
This document discusses high performance liquid chromatography (HPLC). It begins by defining HPLC and explaining how it provides higher efficiency separations compared to classical liquid chromatography due to its use of higher pressures and smaller stationary phase particles. The two main types of HPLC are then described as normal phase and reversed phase. The key components of an HPLC instrument are outlined including solvent reservoirs, pumps, sample injection systems, columns, temperature controllers, and detectors. Details are provided about each of these components and how they function within the overall HPLC system.
Ultra high performance liquid chromatography (UHPLC) provides faster, more sensitive and higher resolution separations compared to traditional high performance liquid chromatography (HPLC). UHPLC uses columns packed with smaller particles less than 2um in diameter which allows for higher pressures and flow rates. This leads to significantly shorter run times, lower detection limits, and better resolution of peaks. The key components of a UHPLC system include pumps that can handle higher pressures, injection systems with low dwell volumes, specialized columns, and detectors capable of measuring small changes. UHPLC has applications in areas like pharmaceutical analysis, metabolomics, and impurity profiling where high resolution and sensitivity are important.
The presentation contains basic introduction to mostly used and versatile reversed phase chromatography, its instrumentation, working and applications. It will be useful for you to understand basic concepts about RP-HPLC.
High Performance Liquid Chromatography (HPLC) is presented. HPLC is a chromatographic technique used to separate mixtures by using high pressure to force a liquid mobile phase and sample through a column packed with solid stationary phase. Key aspects summarized include:
1. HPLC provides simultaneous analysis, high resolution, sensitivity, repeatability for qualitative and quantitative analysis.
2. It works on principles of adsorption and partition chromatography depending on the stationary phase.
3. Instrumentation includes pumps, injector, analytical column, detector, and recorder/integrator.
4. Parameters like retention time, capacity factor, separation factor, and plate height provide information about sample separation and column efficiency.
High performance liquid chromatography (HPLC) is a widely used technique to separate mixtures of organic compounds. HPLC works by partitioning components of a mixture between a stationary phase made of small particles in a steel column and a mobile phase solvent. Pressure is required to force the mobile phase through the stationary phase due to the small particle size. HPLC provides enhanced separations in shorter time periods compared to traditional liquid chromatography. A variety of stationary and mobile phases can be used to optimize separations of different molecules.
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)Suneal Saini
This document provides an introduction to high performance liquid chromatography (HPLC). It discusses the basic components and principles of HPLC, including the stationary and mobile phases, pumps to move the mobile phase through the column at high pressure, and various detectors used to analyze the separated components as they elute from the column. It also describes the different types of HPLC based on the mode of separation, elution technique, scale of operation, and type of analysis performed.
This document provides a detailed overview of the basic components and functioning of an HPLC system. It describes the main components including the mobile phase reservoir, pump, injector, analytical column, detectors, and sample preparation. It explains the working of various types of pumps, columns, detectors and provides the Beer-Lambert law equation. The document is intended to provide a comprehensive schematic and explanation of an HPLC system and its components.
This document discusses high performance liquid chromatography (HPLC). It begins by defining HPLC and explaining how it provides higher efficiency separations compared to classical liquid chromatography due to its use of higher pressures and smaller stationary phase particles. The two main types of HPLC are then described as normal phase and reversed phase. The key components of an HPLC instrument are outlined including solvent reservoirs, pumps, sample injection systems, columns, temperature controllers, and detectors. Details are provided about each of these components and how they function within the overall HPLC system.
Okay, let me break this down step-by-step:
* We are given absorbance values for known concentrations of trimethoprim and quinine standards at two wavelengths
* Using Beer's law, we can calculate the molar absorptivities (ε) of each at each wavelength
* Then using the absorbance readings of the combined sample, and the ε values, we can calculate the concentrations of each in the sample
* From the concentrations and sample volume, the masses and thus amounts of each in the tablet can be determined
Does this help explain the approach? Let me know if you would like me to show the full working.
This document discusses high-performance liquid chromatography (HPLC), which is a widely used technique for separating and analyzing components in mixtures. It describes the basic components and principles of HPLC, including pumps to pass a pressurized liquid and sample mixture through a column containing stationary phase particles. The components interact differently with the stationary phase and are separated into bands that are then detected and analyzed. Common detectors described are UV-visible, fluorescence, and electrochemical detectors. The document also discusses various modes of operation like isocratic and gradient elution and types of columns and stationary phases used.
Detection Method for Low Level of Potent ToxinsShreyas Patel
This document discusses various analytical methods for detecting low levels of potent toxins and impurities. It describes gas chromatography, high-performance liquid chromatography, and thin-layer chromatography techniques. For GC, it outlines instrumentation components like injectors, detectors, and sample introduction methods. For HPLC, it discusses separation modes, detectors, and instrumentation. TLC is described as using capillary action to separate components based on their affinity for the stationary phase. Various compounds are listed along with example separation and detection methods used for their analysis in different matrices.
High performance liquid chromatography (HPLC)Htet Wai Moe
High performance liquid chromatography (HPLC) is a separation technique used to separate mixtures. It uses columns packed with small particle sizes under high pressure, allowing better separation than traditional liquid chromatography. HPLC involves pumping a mobile phase through a column containing a stationary phase, separating components as they flow through at different rates based on interactions with the phases. Components are then detected and quantified as they exit the column. HPLC provides rapid, sensitive, and precise separation of mixtures and is widely used in fields like pharmaceuticals, chemistry, and environmental analysis.
1) HPLC provides improved performance over classical column chromatography due to smaller particle sizes (<5 microns), higher operating pressures (>4000 psi), and higher column efficiencies (>100,000 theoretical plates per meter).
2) There are two main modes of HPLC separation - normal phase which uses a polar stationary phase and non-polar mobile phase, and reverse phase which uses a non-polar stationary phase and polar mobile phase.
3) Key components of an HPLC system include pumps to deliver the mobile phase at high pressure, injectors to introduce samples, columns packed with stationary phase to perform the separation, and detectors such as UV/Vis to identify eluted components.
UPLC provides faster, more sensitive and efficient separations compared to HPLC by using sub-2 μm particles. It operates at higher pressures of up to 100 MPa. Smaller particle sizes and columns allow for shorter run times, lower detection limits, less solvent usage and improved resolution. Key aspects of UPLC instrumentation include high-pressure binary pumps, low-volume injection systems, 1.7 μm particle columns, and detectors adapted for smaller flow cells like tunable UV detectors. UPLC finds applications in pharmaceutical analysis, natural products analysis, and metabolomics studies.
Super Critical Fluid Chromatography uses carbon dioxide or other gases at high pressures and temperatures as the mobile phase to separate compounds. It has advantages over HPLC like faster analysis times, higher efficiency, and the ability to analyze polar compounds without derivatization. Key components include pumps to deliver the mobile phase, columns containing stationary phases for separation, and detectors compatible with supercritical fluids. SFC finds applications in pharmaceuticals, polymers, fuels, and other industries.
It is a multi-element analysis technique that will separate a sample into its constituent atoms and ions and excite it to a higher energy level.
Cause them to emit light with a distinct wavelength, which will be analyzed.
High Performance Liquid chromatography (HPLC)Unnati Garg
The document provides an overview of high performance liquid chromatography (HPLC). It describes key components of HPLC systems including pumps to deliver solvent at stable flow rates, columns for molecular separation, and detectors for recognizing analytes. The separation principle is based on the distribution of analytes between a mobile liquid phase and stationary column packing material. Different constituents are eluted at different times, achieving separation. HPLC is widely used in pharmaceutical applications such as drug development, production quality control, and stability testing.
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
This document provides an overview of high performance liquid chromatography (HPLC). It discusses the components of an HPLC system including the mobile phase, stationary phase, pump, injection system, column, detector, and recorder. It explains that HPLC uses liquid mobile phases and columns packed with small diameter particles to provide better resolution and faster analysis compared to traditional liquid chromatography. The document also summarizes different HPLC modes like adsorption, partition, ion-exchange, and size exclusion chromatography. It highlights that HPLC systems operate at high pressures using pumps capable of pressures over 5000 psi.
HPLC is used to separate compounds using pumps to move liquid solvents through a column containing solid particles. Samples are injected and the different compounds interact differently with the column, causing them to elute at different retention times. Common detectors measure properties like UV absorption to identify and quantify the separated compounds. HPLC is used in pharmaceutical analysis and other areas to separate and analyze mixtures of chemicals.
This document provides an overview of high-performance liquid chromatography (HPLC). It discusses the different types of stationary and mobile phases used in HPLC, including normal phase and reversed phase. It also describes the basic instrumentation components of an HPLC system, such as pumps, injectors, columns, detectors, and data systems. Various detector types are explained. The document then covers different modes of chromatography used in HPLC, including partition chromatography, gel permeation chromatography, ion exchange chromatography, affinity chromatography, and chiral chromatography.
All you need to know about the HPLC Chromatography!
HPLC (High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture. HPLC separates and purifies compounds according to their polarity. I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Let’s look at the main components involved in HPLC in this video.
I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences. Watch this video for more details.
If you have any doubts: Please leave a comment in the comments section below.
The document provides an overview of high performance liquid chromatography (HPLC). It discusses the history and development of chromatography and HPLC. The basic components of an HPLC system are described, including the solvent reservoir, pump, injector, column, detector, and recorder/integrator. Various techniques are also summarized, such as reverse phase chromatography. The document outlines the principles of HPLC separation and provides examples of applications such as pharmaceutical analysis.
High Performance Liquid Chromatography-HPLC slide.pptAhnaf maznun
HPLC is a liquid chromatography technique used to separate compounds in a solution. It consists of a pump to deliver the mobile phase, an injector to introduce the sample, a column for separation, and a detector. The sample is injected and carried through the column by the mobile phase. Components interact differently with the stationary phase in the column and elute at different retention times, becoming separated. The detector then measures the concentration of each separated component to produce a chromatogram. Selection of the mobile phase, stationary phase, column type and dimensions, and detector depends on the compounds being analyzed.
HPLC is a powerful analytical technique used in pharmaceutical analysis. It provides enhanced separation of compounds in a shorter time period compared to traditional chromatography. HPLC is useful for identifying, quantifying, and purifying individual components in a mixture. It has high performance, high speed, and can efficiently analyze labile compounds, biochemicals, and macromolecules. HPLC finds applications in isolating natural pharmaceutical compounds, controlling microbiological processes, and assaying various drugs and compounds.
This document provides an introduction and overview of high performance liquid chromatography (HPLC). It defines HPLC as a technique used to separate mixtures of compounds through a column. The principal of HPLC is based on adsorption or partition chromatography depending on the stationary phase. The document outlines the key components of an HPLC system including the solvent reservoir, pump, injector, column, detector and recorder. It also discusses stationary and mobile phases, sample injection methods, and types of pumps used in HPLC.
This document provides information about High Performance Liquid Chromatography (HPLC). It defines HPLC as a technique that uses pumps to pass a pressurized liquid mobile phase through a column packed with adsorbent particles. This allows the separation of a sample mixture as its components interact differently with the stationary phase. The document outlines the basic components of an HPLC system including the sample injector, column, detector, and data analysis devices. It also describes various parameters that affect the separation like retention time and factors, temperature control, and types of columns and detectors commonly used.
sem 2 presentationnhh.pptxgggffufjffudjdjdPratik434909
This document discusses pharmaceutical industry development and cGMP requirements. It provides an overview of the pharmaceutical industry and legal requirements. It then summarizes cGMP requirements related to plant surroundings, general requirements, departmental areas including storage, production, quality control, sterile products areas, and ancillary areas. Finally, it discusses plant location and the importance of selecting an appropriate location considering factors like operating costs, physical plant design, and local market access.
Qualification of HPLC & LCMS.pptdjdjdjdjfjkfxPratik434909
This document discusses the qualification process for an HPLC system and LC-MS system. It describes the four parts of qualification for HPLC: design qualification, installation qualification, operational qualification, and performance qualification. Specific tests and acceptance criteria are provided for evaluating the HPLC system's performance qualification, including tests for baseline noise and drift, detector linearity, temperature accuracy and precision, auto sampler carryover, and gradient composition accuracy. The document also summarizes calibration and tuning parameters and processes for qualifying an LC-MS system.
Okay, let me break this down step-by-step:
* We are given absorbance values for known concentrations of trimethoprim and quinine standards at two wavelengths
* Using Beer's law, we can calculate the molar absorptivities (ε) of each at each wavelength
* Then using the absorbance readings of the combined sample, and the ε values, we can calculate the concentrations of each in the sample
* From the concentrations and sample volume, the masses and thus amounts of each in the tablet can be determined
Does this help explain the approach? Let me know if you would like me to show the full working.
This document discusses high-performance liquid chromatography (HPLC), which is a widely used technique for separating and analyzing components in mixtures. It describes the basic components and principles of HPLC, including pumps to pass a pressurized liquid and sample mixture through a column containing stationary phase particles. The components interact differently with the stationary phase and are separated into bands that are then detected and analyzed. Common detectors described are UV-visible, fluorescence, and electrochemical detectors. The document also discusses various modes of operation like isocratic and gradient elution and types of columns and stationary phases used.
Detection Method for Low Level of Potent ToxinsShreyas Patel
This document discusses various analytical methods for detecting low levels of potent toxins and impurities. It describes gas chromatography, high-performance liquid chromatography, and thin-layer chromatography techniques. For GC, it outlines instrumentation components like injectors, detectors, and sample introduction methods. For HPLC, it discusses separation modes, detectors, and instrumentation. TLC is described as using capillary action to separate components based on their affinity for the stationary phase. Various compounds are listed along with example separation and detection methods used for their analysis in different matrices.
High performance liquid chromatography (HPLC)Htet Wai Moe
High performance liquid chromatography (HPLC) is a separation technique used to separate mixtures. It uses columns packed with small particle sizes under high pressure, allowing better separation than traditional liquid chromatography. HPLC involves pumping a mobile phase through a column containing a stationary phase, separating components as they flow through at different rates based on interactions with the phases. Components are then detected and quantified as they exit the column. HPLC provides rapid, sensitive, and precise separation of mixtures and is widely used in fields like pharmaceuticals, chemistry, and environmental analysis.
1) HPLC provides improved performance over classical column chromatography due to smaller particle sizes (<5 microns), higher operating pressures (>4000 psi), and higher column efficiencies (>100,000 theoretical plates per meter).
2) There are two main modes of HPLC separation - normal phase which uses a polar stationary phase and non-polar mobile phase, and reverse phase which uses a non-polar stationary phase and polar mobile phase.
3) Key components of an HPLC system include pumps to deliver the mobile phase at high pressure, injectors to introduce samples, columns packed with stationary phase to perform the separation, and detectors such as UV/Vis to identify eluted components.
UPLC provides faster, more sensitive and efficient separations compared to HPLC by using sub-2 μm particles. It operates at higher pressures of up to 100 MPa. Smaller particle sizes and columns allow for shorter run times, lower detection limits, less solvent usage and improved resolution. Key aspects of UPLC instrumentation include high-pressure binary pumps, low-volume injection systems, 1.7 μm particle columns, and detectors adapted for smaller flow cells like tunable UV detectors. UPLC finds applications in pharmaceutical analysis, natural products analysis, and metabolomics studies.
Super Critical Fluid Chromatography uses carbon dioxide or other gases at high pressures and temperatures as the mobile phase to separate compounds. It has advantages over HPLC like faster analysis times, higher efficiency, and the ability to analyze polar compounds without derivatization. Key components include pumps to deliver the mobile phase, columns containing stationary phases for separation, and detectors compatible with supercritical fluids. SFC finds applications in pharmaceuticals, polymers, fuels, and other industries.
It is a multi-element analysis technique that will separate a sample into its constituent atoms and ions and excite it to a higher energy level.
Cause them to emit light with a distinct wavelength, which will be analyzed.
High Performance Liquid chromatography (HPLC)Unnati Garg
The document provides an overview of high performance liquid chromatography (HPLC). It describes key components of HPLC systems including pumps to deliver solvent at stable flow rates, columns for molecular separation, and detectors for recognizing analytes. The separation principle is based on the distribution of analytes between a mobile liquid phase and stationary column packing material. Different constituents are eluted at different times, achieving separation. HPLC is widely used in pharmaceutical applications such as drug development, production quality control, and stability testing.
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase.
This document provides an overview of high performance liquid chromatography (HPLC). It discusses the components of an HPLC system including the mobile phase, stationary phase, pump, injection system, column, detector, and recorder. It explains that HPLC uses liquid mobile phases and columns packed with small diameter particles to provide better resolution and faster analysis compared to traditional liquid chromatography. The document also summarizes different HPLC modes like adsorption, partition, ion-exchange, and size exclusion chromatography. It highlights that HPLC systems operate at high pressures using pumps capable of pressures over 5000 psi.
HPLC is used to separate compounds using pumps to move liquid solvents through a column containing solid particles. Samples are injected and the different compounds interact differently with the column, causing them to elute at different retention times. Common detectors measure properties like UV absorption to identify and quantify the separated compounds. HPLC is used in pharmaceutical analysis and other areas to separate and analyze mixtures of chemicals.
This document provides an overview of high-performance liquid chromatography (HPLC). It discusses the different types of stationary and mobile phases used in HPLC, including normal phase and reversed phase. It also describes the basic instrumentation components of an HPLC system, such as pumps, injectors, columns, detectors, and data systems. Various detector types are explained. The document then covers different modes of chromatography used in HPLC, including partition chromatography, gel permeation chromatography, ion exchange chromatography, affinity chromatography, and chiral chromatography.
All you need to know about the HPLC Chromatography!
HPLC (High-performance liquid chromatography) also referred as high-pressure liquid chromatography, is a technique used in analytical labs to separate, identify, and quantify each component in a mixture. HPLC separates and purifies compounds according to their polarity. I have tried to simplify the whole process of HPLC Chromatography and explain in simple terms. Let’s look at the main components involved in HPLC in this video.
I discussed about Normal Phase Chromatography, Reverse Phase Chromatography and their comparison and differences. Watch this video for more details.
If you have any doubts: Please leave a comment in the comments section below.
The document provides an overview of high performance liquid chromatography (HPLC). It discusses the history and development of chromatography and HPLC. The basic components of an HPLC system are described, including the solvent reservoir, pump, injector, column, detector, and recorder/integrator. Various techniques are also summarized, such as reverse phase chromatography. The document outlines the principles of HPLC separation and provides examples of applications such as pharmaceutical analysis.
High Performance Liquid Chromatography-HPLC slide.pptAhnaf maznun
HPLC is a liquid chromatography technique used to separate compounds in a solution. It consists of a pump to deliver the mobile phase, an injector to introduce the sample, a column for separation, and a detector. The sample is injected and carried through the column by the mobile phase. Components interact differently with the stationary phase in the column and elute at different retention times, becoming separated. The detector then measures the concentration of each separated component to produce a chromatogram. Selection of the mobile phase, stationary phase, column type and dimensions, and detector depends on the compounds being analyzed.
HPLC is a powerful analytical technique used in pharmaceutical analysis. It provides enhanced separation of compounds in a shorter time period compared to traditional chromatography. HPLC is useful for identifying, quantifying, and purifying individual components in a mixture. It has high performance, high speed, and can efficiently analyze labile compounds, biochemicals, and macromolecules. HPLC finds applications in isolating natural pharmaceutical compounds, controlling microbiological processes, and assaying various drugs and compounds.
This document provides an introduction and overview of high performance liquid chromatography (HPLC). It defines HPLC as a technique used to separate mixtures of compounds through a column. The principal of HPLC is based on adsorption or partition chromatography depending on the stationary phase. The document outlines the key components of an HPLC system including the solvent reservoir, pump, injector, column, detector and recorder. It also discusses stationary and mobile phases, sample injection methods, and types of pumps used in HPLC.
This document provides information about High Performance Liquid Chromatography (HPLC). It defines HPLC as a technique that uses pumps to pass a pressurized liquid mobile phase through a column packed with adsorbent particles. This allows the separation of a sample mixture as its components interact differently with the stationary phase. The document outlines the basic components of an HPLC system including the sample injector, column, detector, and data analysis devices. It also describes various parameters that affect the separation like retention time and factors, temperature control, and types of columns and detectors commonly used.
sem 2 presentationnhh.pptxgggffufjffudjdjdPratik434909
This document discusses pharmaceutical industry development and cGMP requirements. It provides an overview of the pharmaceutical industry and legal requirements. It then summarizes cGMP requirements related to plant surroundings, general requirements, departmental areas including storage, production, quality control, sterile products areas, and ancillary areas. Finally, it discusses plant location and the importance of selecting an appropriate location considering factors like operating costs, physical plant design, and local market access.
Qualification of HPLC & LCMS.pptdjdjdjdjfjkfxPratik434909
This document discusses the qualification process for an HPLC system and LC-MS system. It describes the four parts of qualification for HPLC: design qualification, installation qualification, operational qualification, and performance qualification. Specific tests and acceptance criteria are provided for evaluating the HPLC system's performance qualification, including tests for baseline noise and drift, detector linearity, temperature accuracy and precision, auto sampler carryover, and gradient composition accuracy. The document also summarizes calibration and tuning parameters and processes for qualifying an LC-MS system.
Qualification of HPLC & LCMS.pptxfjddjdjdhdjdjjPratik434909
This document discusses the qualification process for an HPLC system and LC-MS system. It describes the four parts of the HPLC qualification process: design qualification, installation qualification, operational qualification, and performance qualification. Specific tests and acceptance criteria are provided for evaluating the HPLC system's performance qualification, including tests for baseline noise and drift, detector linearity, temperature accuracy and precision, auto sampler carry over, and gradient composition accuracy. The document also discusses calibration and tuning parameters for qualifying an LC-MS system.
The document discusses process automation in the pharmaceutical industry, specifically related to tablet and coated products manufacturing. It covers several key points:
1) Automation aims to increase productivity and quality while reducing costs and risks for workers. In pharmaceuticals, automation ensures compliance with regulations.
2) Automation can be applied at various stages of production from material handling to inspection. It has benefits like improved quality, accuracy and safety but also high costs and risk of shutdowns.
3) In tablet production, automation improves material handling, eliminates steps, and incorporates automated process control of unit operations like tableting using computerized presses. This streamlines production and quality control.
This document provides information on various manufacturing operations and controls for pharmaceutical products. It discusses topics like expiry date determination through stability studies, factors affecting drug stability, calculation of theoretical and actual yields, production record reviews, change control procedures, requirements for sterile production, packaging operations, and reprocessing. The key points are that stability studies are required to set expiry dates, various factors can impact drug stability, yields should be monitored and investigated, production records must be reviewed and approved, changes must be properly controlled, sterile and packaging processes require special controls, and reprocessing may be allowed in some cases.
This document provides information on various manufacturing operations and controls for pharmaceutical products. It discusses topics like expiry date determination through stability studies, factors affecting drug stability, calculation of theoretical and actual yields, production record reviews, change control procedures, requirements for sterile production, packaging operations, and reprocessing. The key points are that stability studies are required to set expiry dates, various factors can impact drug stability, yields should be monitored and investigated, production records must be reviewed and approved, changes must be properly controlled, sterile and packaging processes require special controls, and reprocessing may be allowed in some cases.
The document discusses pilot plant scale up for pharmaceutical manufacturing. It defines key terms like pilot plant and scale-up. The objectives of the pilot plant include standardizing the formula, evaluating equipment, and identifying critical process parameters. The significance is that the pilot plant optimizes production rates and provides information for full-scale manufacturing. Steps in scale-up involve laboratory and pilot studies, process and equipment design, evaluation of results, and determining if full-scale production is viable. The pilot plant must be properly operated, validated, staff trained, and engineering supported to ensure quality and compliance.
This document provides an overview of quality management systems (QMS) and related concepts such as total quality management (TQM) and Six Sigma. It discusses the history and benefits of QMS, the key elements and requirements of a QMS including documentation principles. TQM principles, characteristics, elements and advantages/disadvantages are defined. Six Sigma concepts and methodologies are also introduced. The document aims to guide the reader on QMS and quality standards like ISO 9001:2015.
How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.
How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
Executive Directors Chat Leveraging AI for Diversity, Equity, and InclusionTechSoup
Let’s explore the intersection of technology and equity in the final session of our DEI series. Discover how AI tools, like ChatGPT, can be used to support and enhance your nonprofit's DEI initiatives. Participants will gain insights into practical AI applications and get tips for leveraging technology to advance their DEI goals.
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
The simplified electron and muon model, Oscillating Spacetime: The Foundation...RitikBhardwaj56
Discover the Simplified Electron and Muon Model: A New Wave-Based Approach to Understanding Particles delves into a groundbreaking theory that presents electrons and muons as rotating soliton waves within oscillating spacetime. Geared towards students, researchers, and science buffs, this book breaks down complex ideas into simple explanations. It covers topics such as electron waves, temporal dynamics, and the implications of this model on particle physics. With clear illustrations and easy-to-follow explanations, readers will gain a new outlook on the universe's fundamental nature.
How to Manage Your Lost Opportunities in Odoo 17 CRMCeline George
Odoo 17 CRM allows us to track why we lose sales opportunities with "Lost Reasons." This helps analyze our sales process and identify areas for improvement. Here's how to configure lost reasons in Odoo 17 CRM
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
This presentation includes basic of PCOS their pathology and treatment and also Ayurveda correlation of PCOS and Ayurvedic line of treatment mentioned in classics.
Azure Interview Questions and Answers PDF By ScholarHat
pratik new..........pptx
1. INSTRUMENTATION AND APPLICATION OF HPLC
PREPARED BY: PARMAR PRATIK
GUIDED BY: MS.JENEE CHRISTIAN
ASSISTANT PROFESSOR
FACULTY OF PHARMACY
DHARMSINH DESAI UNIVERSITY, NADIAD
2. HPLC
High Performance Liquid chromatography.
Hplc is the fastest growing analytical technique for analysis of drugs.
The analytical technique in which component of mixture are separated
by ushing high pressure is called hplc or high pressure liquid
chromatography.
It is widely used in industry, research and development center, quality
control laboratories and educational institutions.
4. Solvent Reservior
• Also known as mobile phase.
• Mobile phase is usually organic or aqueous or mixture of both.
• Mobile phase is placed in bottle glass.
Characterstic of mobile phase
• Pure
• Law viscocity
• Law price
• Solubility of the sample
• Chemically inert
5. • The volume capacity of reservoir should be 500ml.
• The reservoir should be placed in plastic container.
6. Tubing
• The nature of tubing used to connect all parts of the system deserve
some attention.
• The inside diameter of diameter of tubing prior to injection device is
not critical but the tubing should be inert, have ability to withstand
pressure and be able to carry sufficient volume.
7. PUMP
• Pump is used for forcing the mobile phase through the column.
• Pump can be constructed from material that are inert to all mobile
phases.
• Material commonly used are glass,stainless steel,Teflon.
• The pump is capable for delivering high pressure upto 500psi at flow
rates of upto 3ml/min for analytical solution.
TYPES OF PUMP
1. Constant-Flow-Rate-Pump
2. Constant -Pressure Pump
8. 1.Constant-Flow-Rate-Pump
• The two principle type of pump in this category are the reciprocating
piston and syringe drive pump.
• The reciprocating piston pump is presently the most widely used pump
in hplc.
• This pump is characterized by a filling and pumping cycle.
• During the filling cycle a piston is with drawn from a syringe type
chamber.
• Two check valve are connected to this chamber such that during the
piston withdrawal solvent flow from the solvent reservoir but not into
pump outlet.
• When the piston direction is reversed the check valve operateto allow
solvent to flow from the outlet valve only.
9. • A principle advantage of reciprocating piston pump is the essentially
unlimited volume of the solvent reservoir since it external to the pump.
• A major disadvantage lies in the potential for producing pulses in the
flow rate.
• A more sophisticated ‘’single-head’’ reciprocating piston pump
(e.g.Altex Model 110A) provides for a very rapid filling cycle thus
minimizing the dead time between the cycle.it produce flow.
• Dual piston pump, operated 180 out of phase, further reduce the
pilsation since one head filling is while the other is discharging giving
flow.
10. 2.Constant-Pressure pump
• Constant pressure pump can deliver a constant-flow-rate if the pump
operates against a constant column back pressure and viscosity of
mobile phase remain constant.
• Consequently the temperature should be controlled.
• The simplest form of pump is reservoir such as coil of tubing to which
pressure is applied from a gas cylinder.
• While this system is simple,is suffer from very limited solvent
capacity.
• Since the mobile phase is direct contact with the pressurized gas a
significant amount of gas may be dissolved.
• Thus a portion of mobile phasemay contain sufficient dissolve gasesto
produce bubbles in the detector.
11. Sample injection system
• The injection of sample onto the column presents some unique
problem because of some unique problem present on hplc .
• Direct sample introduce via syringe in a gas chromatography is
simplest form of injection.
• The syringe must be specifically constructed to withstand pressure
upto1000-1500psi without leakage.
• Syringe injection technique can be used at much high pressure if stop
flow technique are used.
• While mobile phase is flowing the sample can be introduce into the
one chamber of piston .
• The piston is move rapidly to align .
• The most widely used sample injection system is loop injection valve.
12. • Sample is fliused through the sample loop with the excess going to
drain.
• For sample injection the valve is rotated so that mobile phase flows the
sample loop fluishing the sample to the column.
• The rotation of valve may be controlled by air –actuated device.
• A major drawback of this device is that the sample loop must be
changed.
13. Columns and Fittings
• Is the hardware used to connect the various components of lc system.
• The diameter of tubing prior of the sample injection device is
generally not critical.
• Lines connecting the injector to the column and column to detector
must be very small inner diameter in order to minimize extra column
broadening.
• It is good practise to place a 2µm in line filter between the sample
injection device and column.
• This traps particle introduce through the mobile phase or sample.
• Fitting is used to connect column to tubing and tubing to tubing must
be law volume in order to minimize extra-column brand broadening.
14. Detector
• Detector of hplc fall into three general categories.
• Diffential detector or bulk property detector provide a differtial
measurement of a bulk property that is possessed by both solute(analyte)
and mobile phase.
• The refractive index detector falls into this category.
• The solute property or selective detector measure a property of sample
which is not possessed by the mobile phase. Ultraviolet and fluorescence
detector falls into this category.
• The ideal hplc detector would possess several properties.it would have high
sensitivity,produce reoroducuble and predictable response and give a
response to any analyte.
• The detection limit or detecter sensitivity is generally consider to be the
concentration or mass of analyte entering the detector that will be produce a
signal-to-noise ratio of 2.
• A rule of thumb, the detector time constant is not greator than 0.5sec for
high efficiency saparation.
15. Uv-visible detector
• Measure the ability of solute to absorb light at a purticular
wavelength in the ultraviolet (UV) or visible (VIS) wavelength range.
• When light of certain wavelength is directed at flow cell, the
substance inside flow cell absorb the light. As a result, the intensity of
light that leaves the flow cell is less than of the light that enters.
• An absorbance detector measures the extents to which the light
intensity decreases
• All uv visible detector operates on principle of beer’s law.
• Uv-visible detector claasified based as affixed or variable wavelenth
detector.
• The most common wavelenth detector are based on the 254nm line
generated by a law pressure mercury vapour lamp.
16. • By ushing appropriate phosphor, the 254nm cause emission of line at
280nm
• Schematic representation of uv visible hplc detector.
17. Refractive Index Detector
• Refractive index detector or differential refractometers are universal
detector in that they respond to virtually any solute provided that the
refractive index of solute is significantly different from that of the
mobile phase.
• A major disadvantage of refractive index detector is its severe sensitivity
to temperature.
• Temparature of mobile phase, column and detector must be rigorously
controlled if accurate measurement of high sensitivities of 10-5
RIUFS(Refractive index units full scale ±1% noise) are common with
commercial instruments.
• The most common RI detector is deflection refractometer.
• In this detector the beam of light is collimated and focus on detector cell.
18. • The Fresnel refractometer is based on Fresnel’s law of reflection .
• This law states that the percentage of light reflected at a glass-liquid
interface is proportional to the angle of incidence and the refractive
indices of the two substances.
• The interferometric refectometer is based on the shearing
interferometric refractometer.
• In this detector the light beam is spilt by a beam spilter, then
recombined by a collimating lens and beam spilter, and focussed on a
photodetector.
19. Recorders
• Recorders are integraters used in hplc are essentially the same as used
in gc.
20. Application of hplc
• Quality control testing of drugs.
• In qualitative and quantitative analysis.
• Saparation and control of impurities.
• In analysis of biological fluids.
• Stability studies.
• Therapeutic monitoring of drug metabolism studies.
• Industrial application.
• Determination of synthetic intermediates ex: Atenolol.
• Stability testing ex:Acyclovir