نشاط بكتيريا.pptx
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This document discusses Actinomycetes, which are filamentous, Gram-positive bacteria that include the genera Actinomyces, Norcardia, and Streptomyces. Actinomyces israelii is described in more detail, including its characteristics as an anaerobic, Gram-positive, branching rod that is part of the normal human flora in the mouth and genitals. Actinomycosis is discussed as a chronic infection caused by A. israelii that can involve the cervicofacial, thoracic, or abdominal regions and presents as swollen lesions that drain pus. Laboratory diagnosis of actinomycosis involves direct examination of samples for sulfur granules,
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This document discusses the phylum Actinobacteria, focusing on the order Actinomycetales. Key points:
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อาณาจักรมอเนอรา ประกอบด้วย สิ่งมีชีวิตพวกยูคาริโอต ได้แก่ แบคทีเรีย อารีแบคทีเรีย และสาหร่ายสีเขียวแกมน้ำเงิน
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อาณาจักรมอเนอรา ประกอบด้วย สิ่งมีชีวิตพวกยูคาริโอต ได้แก่ แบคทีเรีย อารีแบคทีเรีย และสาหร่ายสีเขียวแกมน้ำเงิน
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- 2. General properties Gram positive branching filaments Slow growers Resemble Corynebacteria, mycobacteria and fungi Include: Actinomyces – anaerobe, normal flora Norcadia – aerobe, saprophyte – PARTIALLY ACID FAST Streptomyces- aerobe, saprophyte
- 3. Gram Positive Filamentous Bacteria
- 4. Actinomyces Genus features • Gram positive branching,or diphtheroid-like bacilli • Non sporing • Anaerobic and require CO2 for growth • Non- acid fast (Carbol fuchsin ) Decolourization. • Lack nuclear membrane • Divided by binary fission • Catalase -ve Species of medical importance • Actinomyces israelii
- 5. Actinomyces
- 6. C diptheriae: Gram stain
- 7. Actinomyces israelii Genus Characteristics : • - Gram-positive • branching rods. • - Anaerobic. • - Non-acid fast. • Habitats: human; normal flora of gingival crevices and female genital tract. • Mode of transmission: endogenous. # Trauma (invade tissue)
- 9. Chronic granulomatous lesion that drains pus via sinus (Sulphur granules)
- 10. Cervico-facial infection (Trauma to mouth or dental abscess)
- 11. Actinomycosis • Based on the site of involvement, the three common forms are cervicofacial, thoracic, and abdominal actinomycosis. • Cervicofacial disease presents as a swollen, erythematosus process in the jaw area (known as "lumpy jaw"): dental trauma or poor oral hygiene
- 12. Actinomycosis • A chronic suppurative and granulomatous disease of the cervico-facial, thoracic or abdominal areas
- 15. 2) Direct examination Detect Sulphur granules
- 16. 3) Direct smear Gram stain (Filamentous branching gram positive bacilli)
- 17. 4) Cultivation Anerobic Thioglycolate broth Brain heart infusion blood agar
- 19. 4) Cultivation Anerobic Colonies identified by Immunofluorescent
- 22. Thank You