Epigenetics is the study of alterations in gene expression that occur without changes to the underlying DNA sequence. Some key mechanisms of epigenetics include DNA methylation, histone modifications, and microRNAs. DNA methylation involves the addition of methyl groups to cytosine bases and typically inhibits gene transcription. Histone modifications like acetylation and deacetylation alter chromatin structure and gene accessibility. MicroRNAs regulate gene expression through RNA interference. Epigenetic factors can be influenced by environmental exposures and differ even between identical twins, helping to explain phenotypic differences. Epigenetics also provides insights into disease development and may be modulated by nutrients.
Epigenetics definition, history of epigenetics, molecular basis of epigenetics, epigenetic modification, tools to study epigenetics, disease linked with epigenetics, DNA methylation demethylation and enzymes regulating DNA methylation
-Basic Concepts in Genetics
-What is Epigenetic?
-History of Epigenetic
-How do epigenetics work?
-Epigenetics and the Environment
-Epigenetic Inheritance
-Epigenetics in Psychiatry
Epigenetics definition, history of epigenetics, molecular basis of epigenetics, epigenetic modification, tools to study epigenetics, disease linked with epigenetics, DNA methylation demethylation and enzymes regulating DNA methylation
-Basic Concepts in Genetics
-What is Epigenetic?
-History of Epigenetic
-How do epigenetics work?
-Epigenetics and the Environment
-Epigenetic Inheritance
-Epigenetics in Psychiatry
Describe how the structure of the DNA double helix was discovered. E.pdfarchanadesignfashion
Describe how the structure of the DNA double helix was discovered. Explain how DNA
ultimately controls the functioning of cells (be specific), and how/why mutations in DNA can
disrupt proper functioning. In chapter 2 you learned that the function of DNA and RNA is
\"information storage.\" Using what you\'ve teamed in Chapter 5, describe in detail the specific
functions of DNA and RNA. Explain the process of transcription. Explain the process of
translation. Briefly describe three types of mutations and explain how certain mutations can be
unrecognizable in an organism while others may have disastrous consequences. Summarize
three ways that genetic engineering is being used in agriculture. Explain three concerns
regarding the use of GMO\'s in agriculture How has genetic engineering technology been
directly applied to human health (most agriculture applications are indirect)? Has it been
successful? How are goals used to make medicine?
Solution
1 The function of DNA? depends to a large extent on its structure. The three-dimensional
structure of DNA was first proposed by James Watson and Francis Crick in 1953. It is one of the
most famous scientific discoveries of all time.
James and Francis used evidence shared by others, particularly Rosalind Franklin and Maurice
Wilkins, to determine the shape of DNA. Rosalind worked with Maurice at King\'s College
London. She beamed X-rays through crystals of the DNA molecule and then used photographic
film to record where the scattered X-rays fell. The shadows on the film were then used to work
out where the dense molecules lie in the DNA. This technique is called X-ray diffraction. The
DNA crystals resulted in a cross shape on the X-ray film which is typical of a molecule with a
helix shape. The resulting X-ray was named Photograph 51 and Maurice shared it with James
and Francis.
In 1953 James Watson and Francis Crick published their theory that DNA must be shaped like a
double helix. A double helix resembles a twisted ladder. Each \'upright\' pole of the ladder is
formed from a backbone of alternating sugar and phosphate groups. Each DNA base? (adenine,
cytosine, guanine, thymine) is attached to the backbone and these bases form the rungs. There
are ten \'rungs\' for each complete twist in the DNA helix.
James and Francis suggested that each \'rung\' of the DNA helix was composed of a pair of
bases, joined by hydrogen bonds?. According to Erwin Chargaff’s rules, A would always form
hydrogen bonds with T, and C with G.
2 - It is not the DNA itself that controls cellular functions, it is the proteins that are coded by the
DNA. The nucleotide sequences that make up DNA are a “code” for the cell to make hundreds of
different types of proteins; it is these proteins that function to control and regulate cell growth,
division, communication with other cells and most other cellular functions. This is why DNA is
said to “carry” or “store” information in the form of nucleotide sequences.
The sequences need to be “d.
Epigenetics is the study, in the field of genetics, of cellular and physiological phenotypic trait variations that are caused by external or environmental factors that switch genes on and off and affect how cells read genes instead of being caused by changes in the DNA sequence. -Wikipedia
Prof. Dr. Vladimir Trajkovski - Epigenetics of ASD-10.05.2019Vladimir Trajkovski
President of MSSA Prof. Dr. Vladimir Trajkovski presented this topic "Epigenetics of Autism Spectrum Disorders" at the mini simposyum in Voerandaal, Holland, organized by ReAttach Academy at May 10th 2019.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Describe how the structure of the DNA double helix was discovered. E.pdfarchanadesignfashion
Describe how the structure of the DNA double helix was discovered. Explain how DNA
ultimately controls the functioning of cells (be specific), and how/why mutations in DNA can
disrupt proper functioning. In chapter 2 you learned that the function of DNA and RNA is
\"information storage.\" Using what you\'ve teamed in Chapter 5, describe in detail the specific
functions of DNA and RNA. Explain the process of transcription. Explain the process of
translation. Briefly describe three types of mutations and explain how certain mutations can be
unrecognizable in an organism while others may have disastrous consequences. Summarize
three ways that genetic engineering is being used in agriculture. Explain three concerns
regarding the use of GMO\'s in agriculture How has genetic engineering technology been
directly applied to human health (most agriculture applications are indirect)? Has it been
successful? How are goals used to make medicine?
Solution
1 The function of DNA? depends to a large extent on its structure. The three-dimensional
structure of DNA was first proposed by James Watson and Francis Crick in 1953. It is one of the
most famous scientific discoveries of all time.
James and Francis used evidence shared by others, particularly Rosalind Franklin and Maurice
Wilkins, to determine the shape of DNA. Rosalind worked with Maurice at King\'s College
London. She beamed X-rays through crystals of the DNA molecule and then used photographic
film to record where the scattered X-rays fell. The shadows on the film were then used to work
out where the dense molecules lie in the DNA. This technique is called X-ray diffraction. The
DNA crystals resulted in a cross shape on the X-ray film which is typical of a molecule with a
helix shape. The resulting X-ray was named Photograph 51 and Maurice shared it with James
and Francis.
In 1953 James Watson and Francis Crick published their theory that DNA must be shaped like a
double helix. A double helix resembles a twisted ladder. Each \'upright\' pole of the ladder is
formed from a backbone of alternating sugar and phosphate groups. Each DNA base? (adenine,
cytosine, guanine, thymine) is attached to the backbone and these bases form the rungs. There
are ten \'rungs\' for each complete twist in the DNA helix.
James and Francis suggested that each \'rung\' of the DNA helix was composed of a pair of
bases, joined by hydrogen bonds?. According to Erwin Chargaff’s rules, A would always form
hydrogen bonds with T, and C with G.
2 - It is not the DNA itself that controls cellular functions, it is the proteins that are coded by the
DNA. The nucleotide sequences that make up DNA are a “code” for the cell to make hundreds of
different types of proteins; it is these proteins that function to control and regulate cell growth,
division, communication with other cells and most other cellular functions. This is why DNA is
said to “carry” or “store” information in the form of nucleotide sequences.
The sequences need to be “d.
Epigenetics is the study, in the field of genetics, of cellular and physiological phenotypic trait variations that are caused by external or environmental factors that switch genes on and off and affect how cells read genes instead of being caused by changes in the DNA sequence. -Wikipedia
Prof. Dr. Vladimir Trajkovski - Epigenetics of ASD-10.05.2019Vladimir Trajkovski
President of MSSA Prof. Dr. Vladimir Trajkovski presented this topic "Epigenetics of Autism Spectrum Disorders" at the mini simposyum in Voerandaal, Holland, organized by ReAttach Academy at May 10th 2019.
Francesca Gottschalk - How can education support child empowerment.pptxEduSkills OECD
Francesca Gottschalk from the OECD’s Centre for Educational Research and Innovation presents at the Ask an Expert Webinar: How can education support child empowerment?
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
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The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
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A Strategic Approach: GenAI in EducationPeter Windle
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This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2. History of epigenetics
- The term epigenetics in its contemporary usage appeared
in the 1990s
- It was used with slightly different meanings
- In 2008 a special concept was reached in epigenetic
inheritance Cold Spring Harbor Laboratory.
- British embryologist Conrad Waddington coined the term
epigenetics in 1942 by analogy to the term phenogenetics
coined by Valentine Hecker.
3. History of epigenetics
- When Waddington coined the term the physical nature of genes
and their role in heredity was unknown Instead he used it as a
conceptual model for how genetic components might interact with
the environment to produce a phenotype.
- Nowadays Waddington's idea of an 'epigenetic landscape' is
firmly established within the context of the system dynamics
approach to the study of cell fate.
4. What is Epigenetics?
A study that investigates alterations to gene expression or phenotype in
an organism that occur without changing the nucleotide sequence of a
gene (DNA).
5. What is Epigenetics?
Epigenetic inheritance explains the manufacture of proteins that
distinguish a particular cell from other cells
-For example: nerve cells make proteins that differ from those made by
other cells in the body although all human body cells have the same
sequence of nucleotides in the DNA molecule.
6. There is a relationship between Epigenetics and
gene expression:
Gene expression can be altered
as follows:
1- active gene That is, activating the gene in
order to build proteins through the process of
transcription and translation
2- silent gene Inhibiting the gene and
preventing the transcription and translation
process, so the protein building process does
not occur
7. The DNA will not
transcription and
translation
The DNA will
transcription and
translation
9. 1- Histone acetylation and deacetylation:
- Histone acetylation: histone acetyltransferase (HATs) adds acetyl groups (Ac) onto histone tails
which results in a nucleosome opening thus allowing for transcription factors to access DNA and
initiate gene transcription.
- Histone deacetylation: histone deacetylases (HDACs) remove acetyl groups (Ac) from the histone
tails, leading to a closed chromatin structure.
10. Acetylation removes the positive charge on the
histones thereby decreasing the interaction of the
N termini of histones (Lysine) with the negatively
charged phosphate groups of (DNA).
As a consequence the condensed chromatin is
transformed into a more relaxed structure that is
associated with greater levels of
gene transcription.
This relaxation can be reversed by
deacetylation catalyzed by HDAC activity.
Relaxed transcriptionally active DNA is referred to
as euchromatin. More condensed (tightly packed)
DNA is referred to as heterochromatin
Condensation can be brought about by processes
including deacetylation and methylation
11. 2- DNA methylation
It involves adding a methyl group to:
the fifth position of the pyrimidine ring of cytosine, generating 5-methylcytosine (5meC)
the number-6 nitrogen of the purine ring of adenine
NOTE: Methylation usually occurs at the gene promoter and typically inhibits gene
transcription ,this change can be inherited through cell differentiation, and it is related to the
pathogenesis of various diseases
14. De novo methylation
is a process by which the addition of methyl groups to unmethylated DNA takes
place at specific CpG sites
Normally CpG sites are unmethylated and DNA methylation occurs in these sites
frequently.
CpG sites are the specific regions of DNA in which cytosine nucleotide is
followed by a guanine nucleotide in the linear sequence of bases along its 5′ → 3′
direction.
De novo methylation is catalyzed by two different methylates: DNMT3A and
DNMT3B.
15. 5-Methylcytosine (5mC) is an important epigenetic modification that serves
as a marker for gene expression X-chromosome inactivation, and
transposon silencing, among other developmental processes
5-meC recognition
16.
17. 3- Micro-RNAs
• Are small noncoding RNAs approximately 18-25 nucleotides in length now recognized as one of the
major regulatory gene families in eukaryotes
• MicroRNAs are transcribed by RNA polymerases II and III
• The regulatory functions of microRNAs are accomplished through the RNA-induced silencing
complex (RISC)
• MicroRNA assembles into RISC, activating the complex to target messenger RNA (mRNA)
specified by the microRNA
• This will either lead to degradation of mRNA strand
18.
19. Study the effect of methyl group on rats
• Two groups of pregnant rat mothers were brought, both carrying embryos
with homozygous genotype (AA) Then a different diet was established for
both mother mice:
The first group: the diet of the mothers in this group contained folic acid, which
is a source of a methyl group so the resulting mice had brown fur and were not
obese (normal).
20. The second group: the diet was devoid of folic acid so the
resulting mice had yellow fur, were obese, and were infected
with other diseases.
21. Conclusion: The scientists explained this (experiment) by saying
that the methyl group contained in the diet of the mice of the first
group represents a factor of epigenetic inheritance.
22. Epigenetic explains the difference in traits between identical
twins as follows:
One of the twins may suffer from certain diseases that the other does not
suffer from
One may become a sporter and the other a painter
They may differ in personal characteristics, such as one being shy,
unlike the other
23. It is true that they carry the same nucleotide arrangement in the
DNA molecule, but the gene expression of each of them differs
because:
1- They may differ in diet
2- They may differ in physical and social activities
3- They may differ in medical care
4- There is an association of epigenetic factors with one of them that
differ from those associated with the other at any stage of their lives
24. Some studies have shown that the older a person gets, the more
differences appear in epigenetic factors between identical twins.
26. Epigenetic inheritance has created a scientific precedent with regard
to explaining the causes of cancer
Epigenetic factors may affect tumor suppressor genes, causing them to
become inactive (silent), which leads to the spread of tumors.
31. • Epigenetics is an inheritable phenomenon that affects gene expression without base pair
changes.
• Epigenetic phenomena include DNA methylation, histone modifications, and chromatin
remodeling.
• Chromatin is quite dynamic and is much more than a neutral system for packaging and
condensing genomic DNA, it is a critical player in controlling the accessibility of DNA for
transcription.
• Modifications of chromatin structure can give rise to a variety of epigenetic effects.
• Due to its reversible character, epigenetics is now considered an attractive field of nutritional
intervention.
Conclusion
32. • During our lifetime, nutrients can modify physiologic and pathologic processes through epigenetic
mechanisms that are critical for gene expression (summarized in Table 1).
• Modulation of these processes through diet or specific nutrients may prevent diseases and maintain health
However, it is very hard to delineate the precise effect of nutrients or bioactive food components on each
epigenetic modulation and their associations with physiologic and pathologic processes in our body,
because the nutrients also interact with genes, other nutrients, and other lifestyle factors.