The document describes the synthesis of isotopically labeled ascorbic acid precursors 13C-3 (L-gulono-1,4-lactone) and 13C-6 (L-galactono-1,4-lactone) through multi-step reactions starting from L-xylose and L-lyxose, respectively. Key steps include the Kiliani-Fischer reaction to extend the carbon backbone and addition of cyanide, followed by acid-catalyzed cyclization to form the labeled lactones. Characterization of the products using NMR spectroscopy and HPLC confirmed the structure and purity of >94.5% for the desired epimers. The labeled compounds will
PAM4 Analysis and Measurement Considerations WebinarHilary Lustig
This webinar explores the acquisition and analysis of PAM4 waveforms. We will show PAM4 Test Configurations, Compliance Measurements and Debug Techniques.
PATH DELAY TEST IN THE PRESENCE OF CROSSTALK,POWER SUPPLY NOISE AND GROUND BO...Kurra Gopi
A circuit that passes delay test must produce correct outputs when inputs
are applied and outputs observed with specied timing.
I For a combinational or synchronous sequential circuit, delay test veries
the limits of delay in combinational logic.
I For correct operation, the delay of the combinational circuit must not
exceed the clock period.
Physical design (PD) issues degrade circuit performance in integrated
circuits.
I They consequently increase the number of path delay defects and detecting
them has become essential in ensuring higher test quality in circuits.
I PD issues are the processes that create additional delays in any circuit
path.
I Path delay defects are usually captured by performing path delay test
using timing-aware ATPG tools.
I Thus, by applying existing path delay test techniques, might not
necessarily identify the right input test patterns.
I This may allow some faults to go undetected. Therefore, PD issues have
to be considered during path delay fault testing.
PAM4 Analysis and Measurement Considerations WebinarHilary Lustig
This webinar explores the acquisition and analysis of PAM4 waveforms. We will show PAM4 Test Configurations, Compliance Measurements and Debug Techniques.
PATH DELAY TEST IN THE PRESENCE OF CROSSTALK,POWER SUPPLY NOISE AND GROUND BO...Kurra Gopi
A circuit that passes delay test must produce correct outputs when inputs
are applied and outputs observed with specied timing.
I For a combinational or synchronous sequential circuit, delay test veries
the limits of delay in combinational logic.
I For correct operation, the delay of the combinational circuit must not
exceed the clock period.
Physical design (PD) issues degrade circuit performance in integrated
circuits.
I They consequently increase the number of path delay defects and detecting
them has become essential in ensuring higher test quality in circuits.
I PD issues are the processes that create additional delays in any circuit
path.
I Path delay defects are usually captured by performing path delay test
using timing-aware ATPG tools.
I Thus, by applying existing path delay test techniques, might not
necessarily identify the right input test patterns.
I This may allow some faults to go undetected. Therefore, PD issues have
to be considered during path delay fault testing.
1H qNMR of EPA and DHA Omega-3 Fatty Acid: PLS-Regression Models Obtained at ...John Edwards
Application of 60 and 300 MHz 1H NMR with PLS-Regression to correlate 1H NMR spectra variability with GC derived EPA and DHA Content in a fish oil nutritional supplement manufacturing process.
EPA Method 200.7, Trace Elements in Water, Solids, and Biosolids by Inductively Coupled Plasma-Atomic Emission Spectrometry, describes the procedure and requirements for multi-element determinations by ICP-AES. This presentation demonstrates the capability of the ICPE-9820, with the ASC-9800 Auto-sampler and the Standard Addition Kit, to produce quick, accurate results that comply with the method.
Total workflow solutions that cater every budget, performance or throughput requirement for confirmatory dioxin analysis were discussed in the Thermo Scientific Lunch Seminar at the Dioxin 2014 conference. D. Hope, CEO & Owner Pacific Rim Laboratoris, presented about the economies of POPs analysis from the point of view of a leading laboratory using the very latest dioxin method kits. C. Cojocariu, Thermo Fisher Scientific, discussed recent changes in EU regulations which bring new opportunities for more labs to participate in dioxin analysis and about validating methods using Gas Chromatography triple quadrupole for PCDD/Fs with reference to the new EU Commission Regulation No. 709/2014.
Lessons learned from injecting liquid activated carbon suspensionAntea Group
Let Antea Group walk you through injecting liquid activated carbon suspension at three service station sites in CA. Topics include beta test results, site geology and chemistry, and lessons learned. Learn more at http://us.anteagroup.com/en-us.
The Determination of Polychlorinated Biphenyls (PCB) Using EPA Method 8082PerkinElmer, Inc.
Polychlorinated biphenyls (PCB) are considered persistent organic pollutants under the Stockholm Convention of Persistent Organic Pollutants and have not been manufactured in the U.S. since they were banned in 1979. They are relatively chemically inert and do not decompose readily, being resistant to oxidation, and are only very slightly soluble in water. Prior to being banned, PCBs were widely used in closed systems such as electrical transformers, capacitors and as heat transfer fluids. They were also used in adhesives, paint, plasticizers and machining oils. Production in the U.S. was wholly from the Monsanto company, who manufactured PCBs under the name Aroclor from 1930 to 1977, using a naming scheme that generally followed a four number convention with the first two digits the number of carbons and the last two digits the percentage of chlorine by mass. Aroclor 1260 (12 carbons and 60% chlorine by mass) was used in electrical equipment up until 1950, when it was replaced by Aroclor 1242 through the next 20 years, until Aroclor 1016 (12 carbons and 42% chlorine) was produced.
Graphene synthesis: Rate and Mechanistic Investigation of Eu(OTf)2-Mediated R...Tufan Ghosh
We describe a fast, efficient, and mild approach to prepare chemically reduced graphene oxide (rGO) at room temperature using divalent europium triflate {Eu(OTf)2}. The characterization of solution-processable reduced graphene oxide has been carried out by various spectroscopic (FT-IR, UV–visible absorption, and Raman), microscopic (TEM and AFM), and powder X-ray diffraction (XRD) techniques. Kinetic study indicates that the bimolecular rate constants for the reduction of graphene oxide are 13.7 ± 0.7 and 5.3 ± 0.1 M–1 s–1 in tetrahydrofuran (THF)–water and acetonitrile (ACN)–water mixtures, respectively. The reduction rate constants are two orders of magnitude higher compared to the values obtained in the case of commonly used reducing agents such as the hydrazine derivative, sodium borohydride, and a glucose–ammonia mixture. The present work introduces a feasible reduction process for preparing reduced graphene oxide at ambient conditions, which is important for bulk production of GO. More importantly, the study explores the possibilities of utilizing the unique chemistry of divalent lanthanide complexes for chemical modifications of graphene oxide.
“Perio-Spardha” Program on Autologous Platelet Concentrates-Two lectures on “Tweaking the Centrifuge- An important protocol in the generation of PRF” and “Applications and Limitations of PRF in Periodontics and Implantology”. Organized by Bangalore Academy of Periodontology (BAP) and Indian Society of Periodontology at Oxford Dental College Hospital and Research Centre, Bangalore, India on 14/02/2017.
Development of a Multi-Variant Frequency Ladder™ for Next Generation Sequenci...Thermo Fisher Scientific
Increasing adoption of NGS has shed light on the need for more
standardized controls to evaluate and optimize system performance.
Samples containing mutations of interest are difficult to source and cell
line pooling experiments to determine limit of detection require significant
investments of time and money. To simultaneously evaluate variant
calling performance in >200 unique amplicons across 50 genes targeted
by NGS tests, AcroMetrix® has developed a proprietary
genomic/synthetic DNA material containing over 550 mutations as a
mixture of SNV’s indels and MNP’s. The limit of detection was then
determined for >400 variants using multiple platforms. Tumor samples
were diluted with matched normal samples to mimic a range of
frequencies. Linearity between the material and diluted tumor tissue
samples were compared. Overall, highly multiplex controls with tunable
frequencies allow for much more extensive, yet streamlined, assay
evaluation and facilitate implementation and impart confidence to NGS
testing.
1H qNMR of EPA and DHA Omega-3 Fatty Acid: PLS-Regression Models Obtained at ...John Edwards
Application of 60 and 300 MHz 1H NMR with PLS-Regression to correlate 1H NMR spectra variability with GC derived EPA and DHA Content in a fish oil nutritional supplement manufacturing process.
EPA Method 200.7, Trace Elements in Water, Solids, and Biosolids by Inductively Coupled Plasma-Atomic Emission Spectrometry, describes the procedure and requirements for multi-element determinations by ICP-AES. This presentation demonstrates the capability of the ICPE-9820, with the ASC-9800 Auto-sampler and the Standard Addition Kit, to produce quick, accurate results that comply with the method.
Total workflow solutions that cater every budget, performance or throughput requirement for confirmatory dioxin analysis were discussed in the Thermo Scientific Lunch Seminar at the Dioxin 2014 conference. D. Hope, CEO & Owner Pacific Rim Laboratoris, presented about the economies of POPs analysis from the point of view of a leading laboratory using the very latest dioxin method kits. C. Cojocariu, Thermo Fisher Scientific, discussed recent changes in EU regulations which bring new opportunities for more labs to participate in dioxin analysis and about validating methods using Gas Chromatography triple quadrupole for PCDD/Fs with reference to the new EU Commission Regulation No. 709/2014.
Lessons learned from injecting liquid activated carbon suspensionAntea Group
Let Antea Group walk you through injecting liquid activated carbon suspension at three service station sites in CA. Topics include beta test results, site geology and chemistry, and lessons learned. Learn more at http://us.anteagroup.com/en-us.
The Determination of Polychlorinated Biphenyls (PCB) Using EPA Method 8082PerkinElmer, Inc.
Polychlorinated biphenyls (PCB) are considered persistent organic pollutants under the Stockholm Convention of Persistent Organic Pollutants and have not been manufactured in the U.S. since they were banned in 1979. They are relatively chemically inert and do not decompose readily, being resistant to oxidation, and are only very slightly soluble in water. Prior to being banned, PCBs were widely used in closed systems such as electrical transformers, capacitors and as heat transfer fluids. They were also used in adhesives, paint, plasticizers and machining oils. Production in the U.S. was wholly from the Monsanto company, who manufactured PCBs under the name Aroclor from 1930 to 1977, using a naming scheme that generally followed a four number convention with the first two digits the number of carbons and the last two digits the percentage of chlorine by mass. Aroclor 1260 (12 carbons and 60% chlorine by mass) was used in electrical equipment up until 1950, when it was replaced by Aroclor 1242 through the next 20 years, until Aroclor 1016 (12 carbons and 42% chlorine) was produced.
Graphene synthesis: Rate and Mechanistic Investigation of Eu(OTf)2-Mediated R...Tufan Ghosh
We describe a fast, efficient, and mild approach to prepare chemically reduced graphene oxide (rGO) at room temperature using divalent europium triflate {Eu(OTf)2}. The characterization of solution-processable reduced graphene oxide has been carried out by various spectroscopic (FT-IR, UV–visible absorption, and Raman), microscopic (TEM and AFM), and powder X-ray diffraction (XRD) techniques. Kinetic study indicates that the bimolecular rate constants for the reduction of graphene oxide are 13.7 ± 0.7 and 5.3 ± 0.1 M–1 s–1 in tetrahydrofuran (THF)–water and acetonitrile (ACN)–water mixtures, respectively. The reduction rate constants are two orders of magnitude higher compared to the values obtained in the case of commonly used reducing agents such as the hydrazine derivative, sodium borohydride, and a glucose–ammonia mixture. The present work introduces a feasible reduction process for preparing reduced graphene oxide at ambient conditions, which is important for bulk production of GO. More importantly, the study explores the possibilities of utilizing the unique chemistry of divalent lanthanide complexes for chemical modifications of graphene oxide.
“Perio-Spardha” Program on Autologous Platelet Concentrates-Two lectures on “Tweaking the Centrifuge- An important protocol in the generation of PRF” and “Applications and Limitations of PRF in Periodontics and Implantology”. Organized by Bangalore Academy of Periodontology (BAP) and Indian Society of Periodontology at Oxford Dental College Hospital and Research Centre, Bangalore, India on 14/02/2017.
Development of a Multi-Variant Frequency Ladder™ for Next Generation Sequenci...Thermo Fisher Scientific
Increasing adoption of NGS has shed light on the need for more
standardized controls to evaluate and optimize system performance.
Samples containing mutations of interest are difficult to source and cell
line pooling experiments to determine limit of detection require significant
investments of time and money. To simultaneously evaluate variant
calling performance in >200 unique amplicons across 50 genes targeted
by NGS tests, AcroMetrix® has developed a proprietary
genomic/synthetic DNA material containing over 550 mutations as a
mixture of SNV’s indels and MNP’s. The limit of detection was then
determined for >400 variants using multiple platforms. Tumor samples
were diluted with matched normal samples to mimic a range of
frequencies. Linearity between the material and diluted tumor tissue
samples were compared. Overall, highly multiplex controls with tunable
frequencies allow for much more extensive, yet streamlined, assay
evaluation and facilitate implementation and impart confidence to NGS
testing.