This study evaluated behaviors and attitudes regarding antibiotic use among 298 adults in Portugal. 14% reported self-medicating with antibiotics. Self-medication was more common among younger, more educated individuals. Most self-medicators believed it was acceptable to keep leftover antibiotics for future use. Additionally, 59% thought antibiotics could treat viral infections like colds and flu. The study concludes public health education is needed in Portugal to improve antibiotic management and knowledge of appropriate uses.