Plants in tissue culture slideshare

Plant tissue culture is the process of culturing plant cells, tissues or organs in a nutrient medium under sterile conditions. It has many applications in agriculture including producing rare hybrids, disease-free plants through embryo culture, and micropropagation for vegetative propagation. The key steps involve selecting an explant, sterilizing it, inoculating it in a nutrient medium, initiating callus growth, subculturing, regenerating plantlets, and hardening them for transfer. Plant tissue culture offers advantages like rapid multiplication of plants with optimal traits but has disadvantages like lack of genetic variation. It has potential to commercialize important crops and ensure food security through breeding improved varieties.