A Simple Rp- HPLC Method for Simultaneous Estimation of Six Cardiovascular Dr...iosrjce
A simple, convenient Rp-HPLC method has been developed and validated for the simultaneous
estimation of Metolazone, Indapamide, Nebivolol, Rosuvastatin, Olmesartan and Spironolactone. The column
used was an Inertsil ODS 3 V column of 250 mm length × 4.6 mm ID, with 3 micron particle size of adsorbent.
Separation was achieved using isocratic elution in a buffer-acetonitrile-methanol mobile phase at a flow rate of
1.2 ml/min. The detection was performed at wavelength of 225 nm using a UV detector. The column temperature
was 450C and injection volume was 20µl. The method was validated for precision, linearity and accuracy. The
% RSD for all the drugs was found to be less than 2 %. The correlation coefficient (r2
) was not less than 0.999
for all drugs. The mean percent recovery of the drugs from tablet placebo at 50%, 100% and 150% were within
limits. The marketed formulations of the drugs were analyzed and the mean assay results were found to be
within limits. The developed method can thus be employed for routine simultaneous analysis of Metolazone,
Indapamide, Nebivolol, Rosuvastatin, Olmesartan and Spironolactone in bulk and in their marketed
formulations
Shimadzu has made a method scouting system with UHPLC for the purpose of making method development more efficient. Shimadzu applied this technology to the method development of:
- Non-steroidal anti-inflammatory drugs (NSAIDs)
- Cefem antibiotics
Here, we report on the process of high-efficiency method development of three chiral compounds:
-Bromacil
- α-Methyl-α-Acethyl-γ-Butylrolactone
- Methylclothiazide
For more information, go to ssi.shimadzu.com and follow us on Twitter at @shimadzussi. Thanks for viewing!
Development and Validation of Novel RP-HPLC method for the estimation of Nalo...Bhavana Gundavarapu
The RP-HPLC method was developed and validated for quantitative determination of Naloxegol in pharmaceutical dosage forms. The method was validated with regard to linearity, accuracy, precision, selectivity, and robustness. The method was applied successfully for the determination of Naloxegol during kinetic studies in aqueous solutions (pH and thermal degradation).
A Simple Rp- HPLC Method for Simultaneous Estimation of Six Cardiovascular Dr...iosrjce
A simple, convenient Rp-HPLC method has been developed and validated for the simultaneous
estimation of Metolazone, Indapamide, Nebivolol, Rosuvastatin, Olmesartan and Spironolactone. The column
used was an Inertsil ODS 3 V column of 250 mm length × 4.6 mm ID, with 3 micron particle size of adsorbent.
Separation was achieved using isocratic elution in a buffer-acetonitrile-methanol mobile phase at a flow rate of
1.2 ml/min. The detection was performed at wavelength of 225 nm using a UV detector. The column temperature
was 450C and injection volume was 20µl. The method was validated for precision, linearity and accuracy. The
% RSD for all the drugs was found to be less than 2 %. The correlation coefficient (r2
) was not less than 0.999
for all drugs. The mean percent recovery of the drugs from tablet placebo at 50%, 100% and 150% were within
limits. The marketed formulations of the drugs were analyzed and the mean assay results were found to be
within limits. The developed method can thus be employed for routine simultaneous analysis of Metolazone,
Indapamide, Nebivolol, Rosuvastatin, Olmesartan and Spironolactone in bulk and in their marketed
formulations
Shimadzu has made a method scouting system with UHPLC for the purpose of making method development more efficient. Shimadzu applied this technology to the method development of:
- Non-steroidal anti-inflammatory drugs (NSAIDs)
- Cefem antibiotics
Here, we report on the process of high-efficiency method development of three chiral compounds:
-Bromacil
- α-Methyl-α-Acethyl-γ-Butylrolactone
- Methylclothiazide
For more information, go to ssi.shimadzu.com and follow us on Twitter at @shimadzussi. Thanks for viewing!
Development and Validation of Novel RP-HPLC method for the estimation of Nalo...Bhavana Gundavarapu
The RP-HPLC method was developed and validated for quantitative determination of Naloxegol in pharmaceutical dosage forms. The method was validated with regard to linearity, accuracy, precision, selectivity, and robustness. The method was applied successfully for the determination of Naloxegol during kinetic studies in aqueous solutions (pH and thermal degradation).
chromatography - definition. column chromatography, gas chromatography, high pressure liquid chromatography, thin layer chromatography , paper chromatography - definition and application
Chromatography is a biophysical technique that enables separation, identification and purification of the components of a mixture.
The separation is based on the differential partitioning between the mobile phase and the stationary phase.
Mobile phase – solvent, which move through or over the stationary phase.
Stationary phase – either is solid with high surface area or liquid coated on to a solid; stay still.
Column chromatography is a method for separating mixtures of substances in which a liquid or gaseous solution of mixture (mobile phase) is caused to flow through a tube packed with a finely divided solid (stationary phase ), may be coated with absorbent liquid or through a long capillary tube bearing a thin film of adsorbent liquid.The components of the mixture separates based on the adsorption of the solutes of the solution.
Gas chromatography is technique used to separate and purify individual components from mixture of compounds that can be vaporized. The mobile phase is a gas and the components are separated as vapor. The separation is carried out in a column.
High Performance Liquid Chromatography or high pressure liquid chromatography relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid absorbent material.
Thin layer chromatography is a solid liquid adsorption chromatography. The stationary phase is applied as thin layer on a solid support plate with a liquid mobile phase.
Paper chromatography is analytical method used to separate colored chemicals or substances using a paper as the stationary phase. In this, a thick filter paper comprised the support, and water drops settled in its pores made up the stationary liquid phase. It is a liquid – liquid chromatography.
Analytical method development and validation for simultaneous estimationProfessor Beubenz
Brief about analytical method development and validation
Subscribe to the YouTube Channel #Professor_Beubenz
https://www.youtube.com/channel/UC84jGf2iRN5VjwnQqi6qmXg?view_as=subscriber
The drug or drug combination may not be official in any pharmacopoeias.
A proper analytical procedure for the drug may not be available in the literature due to patent regulations.
Analytical methods may not be available for the drug in the form of a formulation due to the interference caused by the formulation excipients.
Analytical methods for the quantitation of the drug in biological fluids may not be available.
Analytical methods for a drug in combination with other drugs may not be available.
The existing analytical procedures may require expensive reagents and solvents. It may also involve cumbersome extraction and separation procedures and these may not be reliable.
This is to help the young researchers to carry out their research in Pharmaceutical Chemistry, Medicinal Chemistry, Drug Design, Lead Optimization areas. I am providing the major important steps in the Synthesis of novel derivatives having some therapeutic potency.
Formulation and evaluation of FDT and HPLC method development of Amlodipine b...pooja deshmukh
formulation and evaluation of fast disintegrating tablet by using superdisintegrants and RP-HPLC method development of prepered tablet of Amlodipine besilate and Candesartan cilexetil
Differential spectrophotometric method for estimation and validation of Verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, beeers law limits 525µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamine in simple, precise and economically and it recommended for the routine drug quality analysis investigation.
Development and Validation of Reversed-phase High-performance Liquid Chromato...BRNSS Publication Hub
A new, reliable, and sensitive reversed-phase high-performance liquid chromatography method has been developed and validated for simultaneous assay of benzoyl peroxide (BPO) and resveratrol. An isocratic separation of BPO and resveratrol was achieved on C18, 250 mm × 4.6 mm I.d., 5 μm particle size columns with a flow rate of 1.2 ml/min and using a UV detector to monitor the elute at 245 nm. The mobile phase consisted of an ammonium acetate (pH 4) and ethanol. Response was a linear function of drug concentration in the range of 10–100 mg/mL range with an R2 of 0.993 for BPO and 10–100 μg/mL range with an R2 of 0.995 for resveratrol, accuracy with percent relative standard deviation of 100.65 ± 0.23 (benzoic peroxide) and 100.48 ± 0.45 (resveratrol) and with a limit of detection and quantification for BPO and resveratrol, respectively. The result of analysis has been validated statistically and by recovery study. The accuracy ranged between 99.65 and 101.91%. The method was found to be precise, reproducible, and rapid.
Stability indicating method development and validation for the simultaneous e...pharmaindexing
Stability indicating method development and validation for the simultaneous estimation of rabeprazole sodium and ketorolac tromethamine in bulk and synthetic mixture by RP-HPLC
chromatography - definition. column chromatography, gas chromatography, high pressure liquid chromatography, thin layer chromatography , paper chromatography - definition and application
Chromatography is a biophysical technique that enables separation, identification and purification of the components of a mixture.
The separation is based on the differential partitioning between the mobile phase and the stationary phase.
Mobile phase – solvent, which move through or over the stationary phase.
Stationary phase – either is solid with high surface area or liquid coated on to a solid; stay still.
Column chromatography is a method for separating mixtures of substances in which a liquid or gaseous solution of mixture (mobile phase) is caused to flow through a tube packed with a finely divided solid (stationary phase ), may be coated with absorbent liquid or through a long capillary tube bearing a thin film of adsorbent liquid.The components of the mixture separates based on the adsorption of the solutes of the solution.
Gas chromatography is technique used to separate and purify individual components from mixture of compounds that can be vaporized. The mobile phase is a gas and the components are separated as vapor. The separation is carried out in a column.
High Performance Liquid Chromatography or high pressure liquid chromatography relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid absorbent material.
Thin layer chromatography is a solid liquid adsorption chromatography. The stationary phase is applied as thin layer on a solid support plate with a liquid mobile phase.
Paper chromatography is analytical method used to separate colored chemicals or substances using a paper as the stationary phase. In this, a thick filter paper comprised the support, and water drops settled in its pores made up the stationary liquid phase. It is a liquid – liquid chromatography.
Analytical method development and validation for simultaneous estimationProfessor Beubenz
Brief about analytical method development and validation
Subscribe to the YouTube Channel #Professor_Beubenz
https://www.youtube.com/channel/UC84jGf2iRN5VjwnQqi6qmXg?view_as=subscriber
The drug or drug combination may not be official in any pharmacopoeias.
A proper analytical procedure for the drug may not be available in the literature due to patent regulations.
Analytical methods may not be available for the drug in the form of a formulation due to the interference caused by the formulation excipients.
Analytical methods for the quantitation of the drug in biological fluids may not be available.
Analytical methods for a drug in combination with other drugs may not be available.
The existing analytical procedures may require expensive reagents and solvents. It may also involve cumbersome extraction and separation procedures and these may not be reliable.
This is to help the young researchers to carry out their research in Pharmaceutical Chemistry, Medicinal Chemistry, Drug Design, Lead Optimization areas. I am providing the major important steps in the Synthesis of novel derivatives having some therapeutic potency.
Formulation and evaluation of FDT and HPLC method development of Amlodipine b...pooja deshmukh
formulation and evaluation of fast disintegrating tablet by using superdisintegrants and RP-HPLC method development of prepered tablet of Amlodipine besilate and Candesartan cilexetil
Differential spectrophotometric method for estimation and validation of Verap...roshan telrandhe
The aimed of current research to development of the simple, rapid and sensitive Differential spectrophotometric method for the estimation of Verapamil in tablet dosage form. In this method two medium was use acid and alkaline and the difference spectrum was calculated. 0.1N HCL and 0.1N NaOH was used in this differential method. The λmax 278, beeers law limits 525µg/ml, regression equation Y= 0.024x-0.009, slope 0.024, intercept 0.09, correlation coefficient (r2) 0.998, %RSD <1.5, % Recovery (Tablet) 100.46% was shows the good efficacy and results. This method future scope in quality control of the verapamine in simple, precise and economically and it recommended for the routine drug quality analysis investigation.
Development and Validation of Reversed-phase High-performance Liquid Chromato...BRNSS Publication Hub
A new, reliable, and sensitive reversed-phase high-performance liquid chromatography method has been developed and validated for simultaneous assay of benzoyl peroxide (BPO) and resveratrol. An isocratic separation of BPO and resveratrol was achieved on C18, 250 mm × 4.6 mm I.d., 5 μm particle size columns with a flow rate of 1.2 ml/min and using a UV detector to monitor the elute at 245 nm. The mobile phase consisted of an ammonium acetate (pH 4) and ethanol. Response was a linear function of drug concentration in the range of 10–100 mg/mL range with an R2 of 0.993 for BPO and 10–100 μg/mL range with an R2 of 0.995 for resveratrol, accuracy with percent relative standard deviation of 100.65 ± 0.23 (benzoic peroxide) and 100.48 ± 0.45 (resveratrol) and with a limit of detection and quantification for BPO and resveratrol, respectively. The result of analysis has been validated statistically and by recovery study. The accuracy ranged between 99.65 and 101.91%. The method was found to be precise, reproducible, and rapid.
Stability indicating method development and validation for the simultaneous e...pharmaindexing
Stability indicating method development and validation for the simultaneous estimation of rabeprazole sodium and ketorolac tromethamine in bulk and synthetic mixture by RP-HPLC
Chromatography is a technique for separating various inorganic and organic compounds. It is one of the separation techniques used as differential migration. It is more advantageous over conventional separating methods such as crystallization, solvent extraction and distillation. The purpose of presentation is to present various chromatographic techniques included a few advanced forms such as FC, HPLC,UPLC and UPCC (Super Critical chromatography).These are rapid forms of chromatographic techniques based on air pressure driven, optimized for rapid and precise separation of an organic compound.
The current study was performed to develop and validate stability indicating high performance liquid chromatography method (RP-HPLC) for determination of ledipasvir (LPR); to identify and characterize its major degradants by liquid chromatographic– tandem mass spectrometric method (LC-ESI-MS).
HPLC: Principle and Maintenance with Applicationijtsrd
High performance liquid chromatography (HPLC) is a significant qualitative and quantitative technique, usually used for the estimation of pharmaceutical and biological samples. The chromatography term is derived from the Greek words namely chroma (colour) and graphein (to write). The chromatography is very accepted technique and it is mostly used analytically. It is the most resourceful, safest, reliable and fastest chromatographic technique for the quality control of drug components. This technique involves 2 phases"™ stationary and mobile phases. There are different types of chromatographic techniques. The separation of constituents is based on the variation between the partition coefficients of the two phases. This article is primed with an aim to review different aspects of HPLC, such as principle, types, instrumentation and application with maintenance. Yogesh Kumar | Sayed Md Mumtaz | Mustaq Ahmad"HPLC: Principle and Maintenance with Application" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: http://www.ijtsrd.com/papers/ijtsrd17134.pdf http://www.ijtsrd.com/pharmacy/pharmacology-/17134/hplc-principle-and-maintenance-with-application/yogesh-kumar
If revisions are requested, the authors need to address the reviewer's comments and make the necessary changes to the paper. The revised paper is then resubmitted to the journal or conference for another round of review or evaluation of the journalism research paper.
Stability indicating analytical method development and validation for estimat...SriramNagarajan18
Stability indicating analytical method development and validation for estimation of Sacubitril and Valsartan in bulk and pharmaceutical dosage form using RP-HPLC
Collapsing Narratives: Exploring Non-Linearity • a micro report by Rosie WellsRosie Wells
Insight: In a landscape where traditional narrative structures are giving way to fragmented and non-linear forms of storytelling, there lies immense potential for creativity and exploration.
'Collapsing Narratives: Exploring Non-Linearity' is a micro report from Rosie Wells.
Rosie Wells is an Arts & Cultural Strategist uniquely positioned at the intersection of grassroots and mainstream storytelling.
Their work is focused on developing meaningful and lasting connections that can drive social change.
Please download this presentation to enjoy the hyperlinks!
This presentation, created by Syed Faiz ul Hassan, explores the profound influence of media on public perception and behavior. It delves into the evolution of media from oral traditions to modern digital and social media platforms. Key topics include the role of media in information propagation, socialization, crisis awareness, globalization, and education. The presentation also examines media influence through agenda setting, propaganda, and manipulative techniques used by advertisers and marketers. Furthermore, it highlights the impact of surveillance enabled by media technologies on personal behavior and preferences. Through this comprehensive overview, the presentation aims to shed light on how media shapes collective consciousness and public opinion.
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