![Result BLAST
endolysin phage M-102
•lysin [Streptococcus mutans]
•NCBI Reference Sequence: WP_002287207.1
•LOCUS WP_002287207 171 aa linear BCT 12-MAY-2013
•DEFINITION lysin [Streptococcus mutans].
•ACCESSION WP_002287207 VERSION WP_002287207.1 GI:488215999
•SOURCE Streptococcus mutans
•ORGANISM Streptococcus mutans Bacteria; Firmicutes; Bacilli; Lactobacillales;
Streptococcaceae; Streptococcus.
•COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which
may be annotated on many different RefSeq genomes from the same, or different, species.
•FEATURES Location/Qualifiers source 1..171 /organism="Streptococcus mutans"
/db_xref="taxon:1309" Protein 1..171 /product="lysin" /calculated_mol_wt=18727 Region
54..165 /region_name="CHAP" /note="CHAP domain; pfam05257" /db_xref="CDD:218523"
•ORIGIN 1
•mlkktltflg lsvgllivsp hanahtsrlt ldqtnelytr laaegrgvdt dqqygmqcvd 61 idtdltnnyv gvpisgnaid
lldsaraagy eivpaskppr agdlfvmdts tvyghpfght 121 gyiyrvnpdg sfetveqnvg ddsnlytgtv
akfmhrtrdy mlgyirlayr k](https://image.slidesharecdn.com/phagosodonto-131205101953-phpapp02/85/Phagos-odonto-25-320.jpg)
Uploaded byIgor Alves
Phagos odonto
1) The document discusses using bacteriophages and their lysin proteins as a potential approach for dental medicine. It explores using synthetic bacteriophages engineered with lysin genes as a targeted treatment for the oral pathogen Streptococcus mutans, a primary cause of tooth decay. 2) The background provides information on dental caries as the most prevalent chronic disease. It identifies S. mutans as the most important bacteria associated with tooth decay. 3) The solution proposes engineering a synthetic bacteriophage containing a lysin gene under a strong promoter for targeted killing of S. mutans. The engineered lysin would destroy the cell wall of gram-positive S. mutans bacteria.
Phagos odonto
- 1. La Thérapie parles Bactériophages Approche par la Médecine Dentaire BIF7000 – UQAM 2013 •Patricia Morales •Natalia Samus •Igor Lemos •Deraldo Duran (Lisbon University)
- 2.
- 3. BACKGROUND Problématique Streptococcus mutans • Mostimportant bacteria associated with tooth decay, • Naturally present in the human oral microbiota
- 4.
- 5. BACKGROUND Problématique Dental caries Periodontal diseasesand dental caries are the most prevalent chronic diseases, affecting children, adolescents, adults and the elderly. In fact, dental caries affects 60% to 90% of schoolchildren and the vast majority of adults in most industrialized countries. http://www.phac-aspc.gc.ca/publicat/cdicmcbc/30-4/preface-eng.php#ref_05
- 6. BACKGROUND Problématique Dental caries Endodontic therapy •In certain cases, endodontic therapy may be necessary for the restoration of a tooth
- 7. BACKGROUND Problématique Dental caries Endodontic therapy Prostheticteeth Dental surgery
- 8.
- 9. BACKGROUND Problématique Efficacy of dentalfloss in addition to a toothbrush ?????????????? X In fact, dental caries affects 60% to 90% of schoolchildren and the vast majority of adults in most industrialized countries.
- 10.
- 11. Phage Therapy Phage therapyis the therapeutic use of bacteriophages to treat pathogenic bacterial infections 1:n Lysin Streptococcus mutans
- 12. Phage Therapy 1 -Bacteriophage X 1:n n - Bacteries Chain Reaction
- 13. Phage Therapy Solution 1 -Bacteriophage 1:1 1 - Bacteria No Chain Reaction
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- 15.
- 16. Lysin destroys acell wall of grampositive bacteria (Streptococcus) Lysin Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan Peptidoglycan
- 17. PHAST(PHAge Search Tool)- web server designed to identify, annotate and graphically display prophage sequences within bacterial genomes or plasmids 10947 Genomes Databases for download: vIrus DB (57.902 Mb). Last update: Nov 1 2013 Prophage and virus DB (58.039 Mb). Last update: Nov 1 2013 Bacteria DB (2.626 Gb). Last update: Nov 1 2013 PHAST result DB (tar zip format) (104.167 Mb). Last update: Nov 1 2013
- 18. Pathologic Streptococci (compete genom) NCnumber strain PHAGE # Str. mutans NC_004350.1 UA159 1 Str. mutans AP010655.1 NN2025 0 Str. mutans CP003686.1 GS-5 0 Str. criceti AEUV00000000 HS-6 - Str. ratti - - - Str. sobrinus - - - Str. downei AEKN00000000.1 F0415 1 Str. ferus - - - Str. macacae AEUW00000000 NCTC 11558 - Allan L. Delisle, Ming Guo, Natalia I. Chalmers, et al Appl Environ Microbiol. 2012 Apr;78(7):2264-71
- 19. Streptococcus mutans UA159 completegenome Genes de phages
- 20. Streptococcus mutans UA159 genome,phage genes
- 21. Streptococcus mutans UA159, genesof phages # CDS_POSITION BLAST_HIT E-VALUE SEQUENCE 1 complement(1777567..17 78640) PHAGE_Entero_phiV10: O-acetyltransferase; SMU_1884c; phage(gi89152472) 4e-16 Click 2 complement(1781127..17 81393) PHAGE_Human__2: EBNA-1; SMU_1889c; phage(gi139424506) 5e-06 Click 3 complement(1781999..17 82835) PROPHAGE_Escher_MG1655: IS150 transposase B; SMU_1893c; phage(gi16131429) 8e-74 Click 4 complement(1782829..17 83347) PROPHAGE_Ralsto_GMI1000: ISRSO11-transposase ORFA protein; SMU_1894c; phage(gi17546156) 2e-07 Click 5 complement(1783662..17 83964) PHAGE_Staphy_PT1028: ORF011; SMU_1896c; phage(gi66395175) 4e-07 Click 6 1784304..1784786 PHAGE_Bacill_SPBc2: ABC transporter; SMU_1897; phage(gi9630145) 3e-07 Click 7 1784881..1786392 PHAGE_Bacill_SPBc2: ABC transporter; SMU_1898; phage(gi9630145) 5e-62 Click 8 1786502..1787515 PHAGE_Megavi_lba: hypothetical protein; SMU_1900; phage(gi448825457) 1e-12 Click 9 complement(1788212..17 89234) PHAGE_Equid__9: envelope glycoprotein J; SMU_1904c; phage(gi216905924) 5e-05 Click
- 22. Streptococcus downei F0415 genome,phage genes
- 23.
- 24. Putative endolysin, phage M-102 Definition: Streptococcusphage M102 Accession: NC_012884 Length: 31147 bp LOCUS_TAG M102_gp20 ORF START 20879 ORF STOP 21394 STRAND Forward REGION 1 REGION intact or incomplete 39.25 PROTEIN SEQUENCE MLKKTLAILGLSASLLFVSAHANAHTSRLTLDQTNELYTRLAAEGRGVDT DQVYGMQCVD IDTDLTNNYVGIPISGNAIDLLDSARRAGYEIVPANKPPRAGDLFVMDTN ALYGHPFGHT GYVYKVNPDGSFETVEQNVGDDSNFYTGTVAKFMHRTRDYMLGYIRLA YRK HOMOLOG/ORTHOLOG SPECIES PHAGE_Strept_M102 HOMOLOG/ORTHOLOG PROTEIN putative endolysin; phage(gi242345591) HOMOLOG/ORTHOLOG EVALUE 1e-96
- 25. Result BLAST endolysin phageM-102 •lysin [Streptococcus mutans] •NCBI Reference Sequence: WP_002287207.1 •LOCUS WP_002287207 171 aa linear BCT 12-MAY-2013 •DEFINITION lysin [Streptococcus mutans]. •ACCESSION WP_002287207 VERSION WP_002287207.1 GI:488215999 •SOURCE Streptococcus mutans •ORGANISM Streptococcus mutans Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus. •COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. •FEATURES Location/Qualifiers source 1..171 /organism="Streptococcus mutans" /db_xref="taxon:1309" Protein 1..171 /product="lysin" /calculated_mol_wt=18727 Region 54..165 /region_name="CHAP" /note="CHAP domain; pfam05257" /db_xref="CDD:218523" •ORIGIN 1 •mlkktltflg lsvgllivsp hanahtsrlt ldqtnelytr laaegrgvdt dqqygmqcvd 61 idtdltnnyv gvpisgnaid lldsaraagy eivpaskppr agdlfvmdts tvyghpfght 121 gyiyrvnpdg sfetveqnvg ddsnlytgtv akfmhrtrdy mlgyirlayr k
- 26.
- 27. Lysin A, phage PH-10 Definition: Streptococcusphage PH10 Accession: NC_012756 Length: 31276 bp LOCUS_TAG PH10_gp51 ORF START 29525 ORF STOP 30529 STRAND Forward REGION 1 REGION intact or incomplete 39.53 PROTEIN SEQUENCE MKKNDLFIDVSSHNGYDITGILEQMGTTNTIIKISESTSYMNPCLSAQVEQSNPIGFYHFAWFG GDIEEAEREARYFLDNVPQKVKYLCLDYEDHASGDKQANTDACIRFMEILKENGYEPIYYSYKP FTLNNIYYEQILEKFPNSLWIAGYGLNDGTADFEYFPSMDGIRWWQYSSNPYDKNIVLLDDEE AKPKWKRNDTGWWYEYPDGSYPKEEWEKIDGTWYYFNERGYSIASRWLKDDGKWYYLKE NGAMAVGWVLVNGKWYYLDASGAMVTGWVQYKDKLYHFKEENGEMSSKEL VKVEGGWYYVNEDGSRSDKPALDVLPDGLIVTTK HOMOLOG/ORTHOLOG SPECIES PHAGE_Strept_PH10 HOMOLOG/ORTHOLOG PROTEIN lysA; phage (gi238821368)
- 28.
- 29. Résultats •En utilisant labase de données de PHAST nous avons trouvé rapidement deux Streptococcus pathogènes (Str. mutants et Str. downei) les quels contiennent gènes de phages dans ses génomes. •On a trouvé parmi ces deux bacteriophages specifiques de streptococcus mutants (M-102 and PH-10), le gène qui contiennent la protéine lysin, la plus active des protéines antimicrobiennes. •Ainsi les protéines de ces phages pourraient être concernés comme des agents potentiels contre les streptocoques pathogènes pour d'autres études.