Spotlight on Applications is a quarterly e-zine compendium, delivering a variety of topics that address the pressing issues and analytical challenges you may face in your application areas today.
This Special Edition features a broad range of applications within Food Safety, Quality/Conformance/Authenticity, as well as Nutrition/Labeling, which you will be able to access at your convenience. Each application in the table of contents includes an embedded link which takes you directly to the appropriate page within the e-zine.
Spotlight on Analytical Applications e-Zine - Volume 2PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 2 includes applications for Energy, Environmental, Food & Beverage, Materials Characterization and Pharmaceuticals.
Fermented foods are finding artisan, regional and chef-driven takes. Their authenticity is rooted in hundreds of years of craft preparation and artisan pride. Regional preparations of fermented foods, like craft pickles and small-batch whiskey, are being elevated in the preferences of consumers looking for artisan foods. In addition to growing artisan appreciation of these foods, more consumers are preferring foods that contain ingredients geared toward wellness. The probiotic goodness of some of these foods, such as kimchi, lactic-acid fermented craft pickles and miso, are appealing to those looking to boost digestive health. Propelled by culinary interest in umami flavor, also known as the fifth taste, fish sauce and dried shrimp are seeing larger roles as ingredients in the kitchens of popular restaurants and foodservice operations as they carve their path toward consumer homes.
Packaged Facts’ new report explores seven different foods and beverages that encompass thematic avenues of opportunity for food businesses. Fermented Artisanal Foods: Culinary Trend Tracking Series charts how current lifestyle and demographic shifts open up fresh menu and packaged food opportunities related to foods fermented with care, which extends the potential for innovation deeper into meal, snack and beverage territory.
Perspectives on three types of foods that are part of the Chinese American diaspora experience and identity. This approach probably applies to other ethnic groups, as well.
Presentation given at the Food & Beverage Conference in Athens, November 2008. Based on insights research done in Greece, we present 10 topics to (make you) think about, with a focus on customer centric marketing and innovation.
You can download this presentation over at www.slideshare.net/futurelab
I am available for speeches and presentations at your event.
Food, Wine & Tourism Marketing Presentationleannerice2008
This document discusses food and wine tourism marketing. It defines tourism marketing and food and wine tourism. Food tourism involves visiting food producers, festivals, or restaurants for food-related experiences. Wine tourism focuses on visiting vineyards, wineries, or festivals centered around wine. The target market for food and wine tourism includes rural tourists, culinary tourists, and gastro tourists seeking authentic cultural and food experiences. Successful marketing utilizes segmentation and applies the services marketing mix. Festivals are an important promotional tool that attract repeat visitors and build customer loyalty to a destination. Case studies examine the growth of food and wine tourism in the UK and example companies that organize food-focused holidays.
This document discusses food authenticity testing and the issues with current testing methods. It introduces next generation sequencing (NGS) as a new method for food authenticity testing. NGS allows for the simultaneous detection of thousands of potential food contaminants in a single test, overcoming limitations of current polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) methods. NGS involves amplifying DNA from all species in a mixed sample, sequencing the amplified products, and comparing the sequences to a reference database to identify contaminants. While a major improvement, NGS also has limitations, such as an inability to currently quantify contamination levels.
This document provides information about the growing local food movement and interest in food provenance. It includes statistics on food blogs and local food websites, examples of restaurants highlighting local producers in their menus, articles about butchers and minimizing meat consumption, and data showing millennials' willingness to purchase organic even during an economic downturn. It also discusses trends toward accountability and traceability in the food system.
Spotlight on Analytical Applications e-Zine - Volume 2PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 2 includes applications for Energy, Environmental, Food & Beverage, Materials Characterization and Pharmaceuticals.
Fermented foods are finding artisan, regional and chef-driven takes. Their authenticity is rooted in hundreds of years of craft preparation and artisan pride. Regional preparations of fermented foods, like craft pickles and small-batch whiskey, are being elevated in the preferences of consumers looking for artisan foods. In addition to growing artisan appreciation of these foods, more consumers are preferring foods that contain ingredients geared toward wellness. The probiotic goodness of some of these foods, such as kimchi, lactic-acid fermented craft pickles and miso, are appealing to those looking to boost digestive health. Propelled by culinary interest in umami flavor, also known as the fifth taste, fish sauce and dried shrimp are seeing larger roles as ingredients in the kitchens of popular restaurants and foodservice operations as they carve their path toward consumer homes.
Packaged Facts’ new report explores seven different foods and beverages that encompass thematic avenues of opportunity for food businesses. Fermented Artisanal Foods: Culinary Trend Tracking Series charts how current lifestyle and demographic shifts open up fresh menu and packaged food opportunities related to foods fermented with care, which extends the potential for innovation deeper into meal, snack and beverage territory.
Perspectives on three types of foods that are part of the Chinese American diaspora experience and identity. This approach probably applies to other ethnic groups, as well.
Presentation given at the Food & Beverage Conference in Athens, November 2008. Based on insights research done in Greece, we present 10 topics to (make you) think about, with a focus on customer centric marketing and innovation.
You can download this presentation over at www.slideshare.net/futurelab
I am available for speeches and presentations at your event.
Food, Wine & Tourism Marketing Presentationleannerice2008
This document discusses food and wine tourism marketing. It defines tourism marketing and food and wine tourism. Food tourism involves visiting food producers, festivals, or restaurants for food-related experiences. Wine tourism focuses on visiting vineyards, wineries, or festivals centered around wine. The target market for food and wine tourism includes rural tourists, culinary tourists, and gastro tourists seeking authentic cultural and food experiences. Successful marketing utilizes segmentation and applies the services marketing mix. Festivals are an important promotional tool that attract repeat visitors and build customer loyalty to a destination. Case studies examine the growth of food and wine tourism in the UK and example companies that organize food-focused holidays.
This document discusses food authenticity testing and the issues with current testing methods. It introduces next generation sequencing (NGS) as a new method for food authenticity testing. NGS allows for the simultaneous detection of thousands of potential food contaminants in a single test, overcoming limitations of current polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) methods. NGS involves amplifying DNA from all species in a mixed sample, sequencing the amplified products, and comparing the sequences to a reference database to identify contaminants. While a major improvement, NGS also has limitations, such as an inability to currently quantify contamination levels.
This document provides information about the growing local food movement and interest in food provenance. It includes statistics on food blogs and local food websites, examples of restaurants highlighting local producers in their menus, articles about butchers and minimizing meat consumption, and data showing millennials' willingness to purchase organic even during an economic downturn. It also discusses trends toward accountability and traceability in the food system.
Food fraud has become a major concern in recent years. Fruit juice is commonly adulterated with cheaper alternatives. High resolution MS in combination with 'omics data analysis approaches can identify markers of adulteration.
Authenticity research and label control are important parts of food and feed safety enforcement. Three examples are provided to illustrate this. First, horse meat was found adulterated in beef products in 2013, which posed food safety issues as horse meat may contain drugs not tested for in beef. Second, marsh mallow herbal tea was found to contain deadly nightshade, hospitalizing consumers. Proper identification is needed for product safety. Third, spectroscopy and pollen profiling can detect artificially produced manuka honey labeled as natural, protecting consumers. A range of analytical methods contribute to analyzing composition and authenticity for enforcing food and feed safety laws and informing consumers.
This document summarizes the key findings from a large global study on brand authenticity. Some of the main points include:
- Authenticity is important to consumers and how they define it may surprise brands. The study revealed an "authenticity deficit" where most consumers do not feel brands are authentic.
- Consumers define authentic brands as those that demonstrate the "3 R's" - Reliable, Respectful, and Real. The Real dimension is the most challenging for many brands.
- Food and beverage brands are seen as the most authentic industry globally, driven by perceptions of reliability. However, all industries can improve on being seen as more "real".
- The study
- Food is connected to identity and authenticity is subjective. An ethnic restaurant's authenticity comes from factors like food quality, decoration, and staff ethnicity rather than just original recipes. Authenticity is negotiated between restaurants adapting for customers and customers from that ethnicity judging authenticity differently. The restaurant owner sees local Greeks who want Greek food as different from tourists, showing how ethnicity shapes views of food and authenticity.
2016; a year in which we will be noticing the transformation the Food & Beverage industry is undergoing even more. Not only on a global but also on a local level, both from a societal and a personal perspective, innovative while traditional at the same time, and even more dynamic and complex than ever. While we have headed into this exciting new year yet, please let us introduce you to the trends we see.
Food fraud is on the increase globally and checking for the authenticity of foodstuffs is a major challenge. Typical of food fraud is the substitution of a low value product for a higher value one to increase profit to the trader. For years, traders have been passing off a lesser quality rice, CSR 30, as the world's finest long-grained, aromatic rice, Basmati, in key markets like the US, Canada and the EU. In the process, the rice exports enjoy the duty exemption accorded to pure Basmati in the EU and thousands of consumers get duped. This presentation describes the use of Atmospheric Pressure Gas Chromatography coupled to high resolution MS to differentiate between types of rice and to identify marker compounds using statistical analysis software.
South American foods and flavors are ripe with opportunity for the food industry. From now-assimilated foods such as quinoa to still exotic imports such as purple corn or aji amarillo, South American cuisines offer unique flavor, color, nutrition, and culinary potential.
At its best, marketing and promoting international and regional cuisines is neither strictly about tradition nor exactly about fusion. It’s about participating in the evolution of foodways that are “always-already” fused, continuing the intermingling of traditions and cross-cultural influences in the spirit of authenticity rather than under the strict letter of culinary law. Peruvian cuisine, for example, is built up not only from indigenous Andean and colonizing Spanish influences, but also from the history of Chinese and Japanese immigration to Peru, manifested in the Chifa and Nikkei expressions of this nation’s cuisine. Menu and product developers will find success with the right balance of respect for tradition and informed innovation. Much of the motivation for looking at regional Mexican cuisines as well as places further south of the border is to bypass the deracinated and overly Americanized Hispanic foods of a past generation. Instead, we can take American food culture back to the future—not simply back to the past— via authentic Latin American foodways.
Profiles in this issue
Looking forward, what’s the opportunity-scape for South American foods and flavors? This report profiles the following menu and packaged product development opportunities:
• Aji peppers and rocoto see expanded applications in sauces and as ingredients for snacks with a global flair
• Purple corn and purple potato will stake more claim as authentic and healing foods, including for those seeking non-GMO ingredients
• In the wake of quinoa, amaranth and pichuberry will play on texture and flavor attributes, as well as leverage health & wellness, to increase penetration in the specialty and natural market
• Pisco and Caipirinha will draw on perennial interest in international spirits and beverages to foster new mixology and flavoring applications
• South American stews, including Brazil’s Feijoada, have gotten their sea legs in the restaurant circles.
Consumer drivers
With a new front-runner and ambassador in Peruvian cuisine, there’s a lot of momentum behind South American cuisine. Consumers are ready for the exploration of these foods and drinks, and to effectively meet this market opportunity restaurants and food retailers must simultaneously respond to the long-term consumer drivers that are reshaping our food culture:
• Health and wellness
• Food authenticity and integrity
• Artisanal and craft spirit
• Purposeful eating
• Flavor adventure and tourism
Behaviour and Attitudes, Ireland's largest independent Market Research company, provided key findings to recent research into the unmet opportunities for tourism SME’s. These opportunities were based around three main trends –
•‘The Savvy Shopper’ – Consumers looking for value
•‘Authenticity’ - Changing consumer tastes and the growing importance of authentic and personal experiences
•‘World of Mouth’ (social media) - The growing importance of social media, particularly with regard to how information is sought and accessed and how purchase decisions are made
The document discusses authenticity and storytelling in branding. It provides several examples of brands that emphasize authenticity by telling distinctive stories about their products and origins. One brand traces each sweater back to the individual sheep that supplied the wool. Another brand sells milk produced locally by smaller farmers in the UK. A tea brand in Turkey emphasizes the unique harvesting process that gives its tea a distinctive taste.
The Seven Secrets to Launching a Megaselling Start-upTom White
This highly visual presentation reveals the secrets behind building your company brand story and launching a start-up that connects with huge audiences.
Spotlight on Analytical Applications e-Zine Environmental Special Edition - V...PerkinElmer, Inc.
Spotlight on Applications is a quarterly e-zine compendium, delivering a variety of topics that address the pressing issues and analytical challenges you may face in your application areas today. This Special Edition features a broad range of applications within Water, Air, Soil/Hazardous Waste testing which you will be able to access at your convenience. Each application in the table of contents includes an embedded link which takes you directly to the appropriate page within the e-zine.
PerkinElmer Spotlight on Analytical Applications e-Zine – Volume 16PerkinElmer, Inc.
Spotlight on Applications is a quarterly compendium of recent applications, delivering a variety of topics that address the pressing issues and analytical challenges you may face today. Our e-zine covers a broad range of applications within various industries – all accessible online at your convenience.
http://www.perkinelmerapplications.com/subscribe/
Spotlight on Analytical Applications e-Zine - Volume 10PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 10 includes applications for Energy & Industrial, Environmental, Food & Beverage, and Pharmaceuticals & Nutraceuticals.
Kristine Houser is seeking employment that utilizes her analytical and technical skills. She is pursuing a Bachelor of Science in Forensic Science from Waynesburg University, expected to graduate in May 2017, with minors in Chemistry and Biology. Her relevant coursework includes genetics, histology, forensic chemistry, serology, biochemistry, instrumental methods, organic chemistry, microscopy, anatomy, calculus, criminal law, and introduction to forensic science. She has laboratory skills in wet-lab procedures, instrumentation operation, and analysis of bodily fluids and chemicals. Her research experience includes using spectroscopy to analyze chemical changes in coffee over time and comparing cafestol concentrations in coffee beans from different regions using HPLC. She also has experience preparing microbi
Application of uv visible spectroscopy in pharmaceutical industryFarhad Ashraf
UV-visible spectroscopy can be used for clinical and pre-clinical applications in cancer diagnosis and analysis. It allows non-invasive measurement of biomarkers like tumor oxygenation, angiogenesis, redox state, and morphology. Spectroscopy measures how light interacts with tissue via absorption and fluorescence of chromophores and fluorophores. This optical signature contains physiologically meaningful information about cancer progression and treatment response that can provide insights not available from traditional pathology. Quantitative optical techniques have the potential to improve cancer management with personalized, molecularly-informed assessments.
Spotlight on Analytical Applications e-Zine - Volume 13PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 13 includes applications for Consumer Products, Energy & Industrial, Environmental and Pharmaceuticals & Nutraceuticals.
This document discusses computer aided sensory evaluation techniques, including e-tongue, e-nose, and texture profile analysis. The e-tongue uses an array of chemical sensors similar to human taste receptors to characterize liquid samples. The e-nose detects smells using an array of gas sensors and pattern recognition. Texture profile analysis uses a texture analyzer to mimic chewing and measure parameters like hardness, cohesiveness, and springiness of foods. Computerized sensory evaluation can reduce bias, save time, and provide consistent results compared to human panels.
This document summarizes a professional development project for high school chemistry teachers. Hands-on workshops were held using various analytical instruments like HPLC, FTIR, fluorescence spectroscopy, and visible light spectroscopy. Teachers were able to identify and quantify aspirin using HPLC and FTIR. They also determined the iron content in samples and monitored vitamin B12 levels in energy drinks. Feedback showed the activities were applicable to teaching and inspired students to study science. Future workshops could cover additional instruments and practices.
This document discusses using k-means clustering to classify Indian black tea samples based on impedance spectroscopy data from an "impedance tongue" sensor array. It provides background on impedance tongue sensors and k-means clustering. The document then describes applying k-means clustering to impedance response data from 120 tea samples measured at different voltages/frequencies, obtaining classification rates from 87-96%. It concludes that k-means clustering is effective for distinguishing tea samples with minimum intra-cluster and maximum inter-cluster distances.
This document discusses using k-means clustering to classify Indian black tea samples based on impedance measurements from an impedance tongue sensor array. It provides background on impedance tongue sensors and k-means clustering. The document then describes applying k-means clustering to impedance measurements of 120 tea samples across 8 classes, obtaining classification rates from 87-96%. It concludes that k-means clustering is effective for distinguishing tea classes with minimum intra-cluster and maximum inter-cluster distances.
Food fraud has become a major concern in recent years. Fruit juice is commonly adulterated with cheaper alternatives. High resolution MS in combination with 'omics data analysis approaches can identify markers of adulteration.
Authenticity research and label control are important parts of food and feed safety enforcement. Three examples are provided to illustrate this. First, horse meat was found adulterated in beef products in 2013, which posed food safety issues as horse meat may contain drugs not tested for in beef. Second, marsh mallow herbal tea was found to contain deadly nightshade, hospitalizing consumers. Proper identification is needed for product safety. Third, spectroscopy and pollen profiling can detect artificially produced manuka honey labeled as natural, protecting consumers. A range of analytical methods contribute to analyzing composition and authenticity for enforcing food and feed safety laws and informing consumers.
This document summarizes the key findings from a large global study on brand authenticity. Some of the main points include:
- Authenticity is important to consumers and how they define it may surprise brands. The study revealed an "authenticity deficit" where most consumers do not feel brands are authentic.
- Consumers define authentic brands as those that demonstrate the "3 R's" - Reliable, Respectful, and Real. The Real dimension is the most challenging for many brands.
- Food and beverage brands are seen as the most authentic industry globally, driven by perceptions of reliability. However, all industries can improve on being seen as more "real".
- The study
- Food is connected to identity and authenticity is subjective. An ethnic restaurant's authenticity comes from factors like food quality, decoration, and staff ethnicity rather than just original recipes. Authenticity is negotiated between restaurants adapting for customers and customers from that ethnicity judging authenticity differently. The restaurant owner sees local Greeks who want Greek food as different from tourists, showing how ethnicity shapes views of food and authenticity.
2016; a year in which we will be noticing the transformation the Food & Beverage industry is undergoing even more. Not only on a global but also on a local level, both from a societal and a personal perspective, innovative while traditional at the same time, and even more dynamic and complex than ever. While we have headed into this exciting new year yet, please let us introduce you to the trends we see.
Food fraud is on the increase globally and checking for the authenticity of foodstuffs is a major challenge. Typical of food fraud is the substitution of a low value product for a higher value one to increase profit to the trader. For years, traders have been passing off a lesser quality rice, CSR 30, as the world's finest long-grained, aromatic rice, Basmati, in key markets like the US, Canada and the EU. In the process, the rice exports enjoy the duty exemption accorded to pure Basmati in the EU and thousands of consumers get duped. This presentation describes the use of Atmospheric Pressure Gas Chromatography coupled to high resolution MS to differentiate between types of rice and to identify marker compounds using statistical analysis software.
South American foods and flavors are ripe with opportunity for the food industry. From now-assimilated foods such as quinoa to still exotic imports such as purple corn or aji amarillo, South American cuisines offer unique flavor, color, nutrition, and culinary potential.
At its best, marketing and promoting international and regional cuisines is neither strictly about tradition nor exactly about fusion. It’s about participating in the evolution of foodways that are “always-already” fused, continuing the intermingling of traditions and cross-cultural influences in the spirit of authenticity rather than under the strict letter of culinary law. Peruvian cuisine, for example, is built up not only from indigenous Andean and colonizing Spanish influences, but also from the history of Chinese and Japanese immigration to Peru, manifested in the Chifa and Nikkei expressions of this nation’s cuisine. Menu and product developers will find success with the right balance of respect for tradition and informed innovation. Much of the motivation for looking at regional Mexican cuisines as well as places further south of the border is to bypass the deracinated and overly Americanized Hispanic foods of a past generation. Instead, we can take American food culture back to the future—not simply back to the past— via authentic Latin American foodways.
Profiles in this issue
Looking forward, what’s the opportunity-scape for South American foods and flavors? This report profiles the following menu and packaged product development opportunities:
• Aji peppers and rocoto see expanded applications in sauces and as ingredients for snacks with a global flair
• Purple corn and purple potato will stake more claim as authentic and healing foods, including for those seeking non-GMO ingredients
• In the wake of quinoa, amaranth and pichuberry will play on texture and flavor attributes, as well as leverage health & wellness, to increase penetration in the specialty and natural market
• Pisco and Caipirinha will draw on perennial interest in international spirits and beverages to foster new mixology and flavoring applications
• South American stews, including Brazil’s Feijoada, have gotten their sea legs in the restaurant circles.
Consumer drivers
With a new front-runner and ambassador in Peruvian cuisine, there’s a lot of momentum behind South American cuisine. Consumers are ready for the exploration of these foods and drinks, and to effectively meet this market opportunity restaurants and food retailers must simultaneously respond to the long-term consumer drivers that are reshaping our food culture:
• Health and wellness
• Food authenticity and integrity
• Artisanal and craft spirit
• Purposeful eating
• Flavor adventure and tourism
Behaviour and Attitudes, Ireland's largest independent Market Research company, provided key findings to recent research into the unmet opportunities for tourism SME’s. These opportunities were based around three main trends –
•‘The Savvy Shopper’ – Consumers looking for value
•‘Authenticity’ - Changing consumer tastes and the growing importance of authentic and personal experiences
•‘World of Mouth’ (social media) - The growing importance of social media, particularly with regard to how information is sought and accessed and how purchase decisions are made
The document discusses authenticity and storytelling in branding. It provides several examples of brands that emphasize authenticity by telling distinctive stories about their products and origins. One brand traces each sweater back to the individual sheep that supplied the wool. Another brand sells milk produced locally by smaller farmers in the UK. A tea brand in Turkey emphasizes the unique harvesting process that gives its tea a distinctive taste.
The Seven Secrets to Launching a Megaselling Start-upTom White
This highly visual presentation reveals the secrets behind building your company brand story and launching a start-up that connects with huge audiences.
Spotlight on Analytical Applications e-Zine Environmental Special Edition - V...PerkinElmer, Inc.
Spotlight on Applications is a quarterly e-zine compendium, delivering a variety of topics that address the pressing issues and analytical challenges you may face in your application areas today. This Special Edition features a broad range of applications within Water, Air, Soil/Hazardous Waste testing which you will be able to access at your convenience. Each application in the table of contents includes an embedded link which takes you directly to the appropriate page within the e-zine.
PerkinElmer Spotlight on Analytical Applications e-Zine – Volume 16PerkinElmer, Inc.
Spotlight on Applications is a quarterly compendium of recent applications, delivering a variety of topics that address the pressing issues and analytical challenges you may face today. Our e-zine covers a broad range of applications within various industries – all accessible online at your convenience.
http://www.perkinelmerapplications.com/subscribe/
Spotlight on Analytical Applications e-Zine - Volume 10PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 10 includes applications for Energy & Industrial, Environmental, Food & Beverage, and Pharmaceuticals & Nutraceuticals.
Kristine Houser is seeking employment that utilizes her analytical and technical skills. She is pursuing a Bachelor of Science in Forensic Science from Waynesburg University, expected to graduate in May 2017, with minors in Chemistry and Biology. Her relevant coursework includes genetics, histology, forensic chemistry, serology, biochemistry, instrumental methods, organic chemistry, microscopy, anatomy, calculus, criminal law, and introduction to forensic science. She has laboratory skills in wet-lab procedures, instrumentation operation, and analysis of bodily fluids and chemicals. Her research experience includes using spectroscopy to analyze chemical changes in coffee over time and comparing cafestol concentrations in coffee beans from different regions using HPLC. She also has experience preparing microbi
Application of uv visible spectroscopy in pharmaceutical industryFarhad Ashraf
UV-visible spectroscopy can be used for clinical and pre-clinical applications in cancer diagnosis and analysis. It allows non-invasive measurement of biomarkers like tumor oxygenation, angiogenesis, redox state, and morphology. Spectroscopy measures how light interacts with tissue via absorption and fluorescence of chromophores and fluorophores. This optical signature contains physiologically meaningful information about cancer progression and treatment response that can provide insights not available from traditional pathology. Quantitative optical techniques have the potential to improve cancer management with personalized, molecularly-informed assessments.
Spotlight on Analytical Applications e-Zine - Volume 13PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 13 includes applications for Consumer Products, Energy & Industrial, Environmental and Pharmaceuticals & Nutraceuticals.
This document discusses computer aided sensory evaluation techniques, including e-tongue, e-nose, and texture profile analysis. The e-tongue uses an array of chemical sensors similar to human taste receptors to characterize liquid samples. The e-nose detects smells using an array of gas sensors and pattern recognition. Texture profile analysis uses a texture analyzer to mimic chewing and measure parameters like hardness, cohesiveness, and springiness of foods. Computerized sensory evaluation can reduce bias, save time, and provide consistent results compared to human panels.
This document summarizes a professional development project for high school chemistry teachers. Hands-on workshops were held using various analytical instruments like HPLC, FTIR, fluorescence spectroscopy, and visible light spectroscopy. Teachers were able to identify and quantify aspirin using HPLC and FTIR. They also determined the iron content in samples and monitored vitamin B12 levels in energy drinks. Feedback showed the activities were applicable to teaching and inspired students to study science. Future workshops could cover additional instruments and practices.
This document discusses using k-means clustering to classify Indian black tea samples based on impedance spectroscopy data from an "impedance tongue" sensor array. It provides background on impedance tongue sensors and k-means clustering. The document then describes applying k-means clustering to impedance response data from 120 tea samples measured at different voltages/frequencies, obtaining classification rates from 87-96%. It concludes that k-means clustering is effective for distinguishing tea samples with minimum intra-cluster and maximum inter-cluster distances.
This document discusses using k-means clustering to classify Indian black tea samples based on impedance measurements from an impedance tongue sensor array. It provides background on impedance tongue sensors and k-means clustering. The document then describes applying k-means clustering to impedance measurements of 120 tea samples across 8 classes, obtaining classification rates from 87-96%. It concludes that k-means clustering is effective for distinguishing tea classes with minimum intra-cluster and maximum inter-cluster distances.
Signature Peptide MRM Optimization Made Easy for Therapeutic Protein and Pept...SCIEX
This technical note describes the results of experiments where DiscoveryQuantTM software was used to optimize compound dependent parameters and improve upon the sensitivity oAf methods obtained from the output of Skyline software for the quantitation of peptides.
Bioanalytical Method Development and Validation for Simultaneous Estimation o...BRNSSPublicationHubI
The document describes the development and validation of a bioanalytical method for the simultaneous estimation of imatinib and its metabolite desmethyl imatinib in human plasma using liquid chromatography-mass spectrometry. Key steps in the method included online enrichment of the analytes followed by separation on a chromatographic column and detection using mass spectrometry. The method was validated in terms of precision, accuracy, selectivity and sensitivity. The developed method was then applied to pharmacokinetic studies of imatinib and its metabolite in patient samples.
Application of UV-vis in natural product researchZarTaj2
Absorbance spectroscopy uses the wavelength-dependent absorption characteristics of materials to identify and quantify specific substances ranging from the visible light to ultraviolet regions. It has various applications in natural product research including determining protein and nucleic acid concentrations by measuring absorbance according to the Lambert-Beer law. It can also monitor enzyme kinetics by measuring changes in absorbance over time. Absorbance spectroscopy is useful for real-time monitoring of bioprocesses and fermentation by measuring optical density and absorbance of biomass and cellular components. It has been used to evaluate phenolic compounds during winemaking.
This document discusses biosensors, including their definition, components, basic characteristics, advantages, sensing techniques, types, and applications. A biosensor is defined as a self-contained integrated device that uses a biological recognition element in direct contact with a transduction element to provide analytical information. Professor Leland C. Clark Jr. is considered the father of biosensors. Biosensors have various advantages such as high specificity, independence from factors like pH, linear response, small size, durability, and rapid results. Common sensing techniques include fluorescence, DNA microarrays, SPR, impedance spectroscopy, and electrochemical methods. Major types include optical, resonant, piezoelectric, ion-sensitive, and electrochemical biosensors. Biosensors
This document describes a student project to monitor water quality in fish farms using a spectrophotometer and IoT sensors. The project aims to measure water parameters like color, oxygen, pH and transmit data in real-time to detect issues. Students conducted experiments adding dyes to water samples and using the spectrophotometer to analyze absorption spectra and correlate intensities at specific wavelengths to color concentration. Multiple linear regression and machine learning models were used to predict color levels. The project plans to develop an autonomous smart buoy with embedded sensors to continuously monitor open water quality.
Spotlight on Analytical Applications e-Zine - Volume 11PerkinElmer, Inc.
This document provides key analytical applications to help laboratories address the pressing concerns of the changing global landscape. Specifically, Volume 11 includes applications for Energy & Industrial, Environmental, Food & Beverage, Pharmaceuticals & Nutraceuticals and Forensics & Toxicology.
SJA CV Training Certificates and Work Presentation 15-Apr-18Shareef Jarvi Antar
Shareef Jarvi Antar has extensive experience developing and validating bioanalytical methods using various analytical techniques such as LC/MS, LC/MS/MS, GC/MS, and ELISA. He has worked for major pharmaceutical companies and CROs developing methods for pharmacokinetic studies, determining drug metabolism and metabolites, and evaluating drug-drug interactions. Currently he is an application chemist for IBL International setting up an LC/MS laboratory and developing automated methods using their equipment.
This thesis describes the synthesis, purification, and stability study of the islet neogenesis-associated protein peptide (INGAP-P) and analogs. INGAP-P and analogs were synthesized using solid phase peptide synthesis. Preliminary identification of the linear and cyclic analogs was done using MALDI-TOF mass spectrometry. Preliminary identification of INGAP-P was done using ESI mass spectrometry. Reverse phase HPLC method development was performed to separate and analyze the peptide mixtures. Parameters such as gradient, column type, and mobile phase composition were optimized. A room temperature degradation study showed relatively rapid degradation of INGAP-P in acidic solvent. Changing the column type from C18 to phenyl improved
The common labels of chemiluminescent immunoassays is alkaline phosphatase (ALP). Huateng Pharma can provides APS-5(CAS#193884-53-6) from milligram to kilogram scale.
The analysis of mycotoxins has become an issue of global interest, in particular because most countries already set up regulative limits or guideline levels for the tolerance of such contaminants in agricultural commodities and products.
Similar to PerkinElmer Analytical Applications E-Zine Food & Beverage Special Edition - Vol. 1 (20)
O documento discute o uso do kit PlGF 1-2-3 da PerkinElmer para triagem da pré-eclâmpsia. O kit permite identificar mulheres com alto risco de pré-eclâmpsia no primeiro trimestre de forma sensível. Quando usado em conjunto com outros fatores, como histórico médico e pressão arterial, pode prever até 88% dos casos de pré-eclâmpsia precoce. O documento também descreve como o tratamento precoce com aspirina em mulheres de alto risco identificadas pela triagem pode reduzir significativamente os casos de pré-
O documento descreve o novo instrumento chemagic Prime, uma solução automatizada para extração de DNA e RNA de amostras humanas. Ele usa esferas magnéticas em vez de placas para separar ácidos nucleicos de forma mais pura e intacta, reduzindo o risco de contaminação. O instrumento fornece extração de alta qualidade de DNA e RNA de várias amostras, como sangue total e tecidos, para aplicações como NGS, genotipagem e PCR.
Por que a triagem para SCID?
A Imunodeficiência combinada grave (SCID) é um grupo de distúrbios caracterizados por um defeito grave na produção e no funcionamento da célula. Normalmente, bebês com SCID falecerão por infecções antes de um ano de idade, a menos que o sistema imunológico do bebê seja restaurado através de tratamento [1].
Solução com marca CE para triagem SCID
A PerkinElmer tem orgulho em apresentar o primeiro ensaio comercial para triagem de TREC. O ensaio faz parte de um sistema completo, com marca CE para garantir o uso seguro e eficaz como um auxiliar na triagem de SCID.
A União Europeia está considerando novas regras para veículos autônomos. As regras propostas exigiriam que os fabricantes de veículos autônomos assumam mais responsabilidade por acidentes e garantam que os sistemas de direção sejam projetados para proteger os pedestres e ciclistas. A Comissão Europeia espera que as novas regras ajudem a promover o desenvolvimento seguro de veículos autônomos na UE.
O cromatógrafo a gás/espectrômetro de massas (GC/MS) portátil Torion® T-9 da PerkinElmer traz novo significado à portabilidade. Pesando apenas 14,5 quilos, este GC/MS também é rápido, confiável e de fácil utilização.
O documento descreve os sistemas Clarus 590/690 GC da Agilent e como eles, juntamente com o software TotalChrom e soluções prontas, podem melhorar a produtividade dos laboratórios ao fornecer recursos fáceis de usar, interface intuitiva de tela sensível ao toque e métodos pré-configurados para aplicações comuns.
A PerkinElmer apresenta uma plataforma automatizada para transferência de líquidos que oferece reprodutibilidade, redução de erros e operação sem necessidade de supervisão contínua. A plataforma possui diferentes tamanhos e configurações para atender às necessidades dos clientes e recursos como seringas, ponteiras descartáveis e software para criação e execução de protocolos de forma simples.
Como a PerkinElmer contribui para diagnósticos mais rápidos?PerkinElmer, Inc.
Uma doença detectada precocemente, pode acelerar a inicialização de tratamentos eficazes, que mudarão a história de vida de crianças portadoras de doenças raras e, de seu núcleo familiar.
Como a PerkinElmer contribui para diagnósticos mais rápidos?PerkinElmer, Inc.
Uma doença detectada precocemente, pode acelerar a inicialização de tratamentos eficazes, que mudarão a história de vida de crianças portadoras de doenças raras e, de seu núcleo familiar.
O documento discute Pré-eclâmpsia, uma condição grave na gravidez caracterizada por pressão arterial elevada que pode ocorrer rapidamente e colocar em risco a vida da mãe e do bebê. A Pré-eclâmpsia afeta 8-10% das gestantes e é uma das principais causas de morte materna e infantil no mundo. Sintomas incluem forte dor de cabeça, dificuldade para respirar, náuseas, alterações de visão, inchaço e ganho de peso rápido. O tratamento precoce com baix
Mais do que nunca, tornou uma premissa para os produtores de carnes, Frigoríficos e Laboratórios, garantirem a segurança e a qualidade dos alimentos que produzem.
Saiba como o DA 7250 pode te ajudar a garantir todos os certificados de segurança e qualidade.
Os kits NEXTflexTM da PerkinElmer fornecem soluções completas para a preparação de bibliotecas para sequenciamento de nova geração (NGS), com protocolos rápidos, alta sensibilidade, flexibilidade e qualidade superior de dados. Os kits estão disponíveis para diversas aplicações como sequenciamento de amplicons de BRCA1 e BRCA2 e RNA pequeno.
O documento descreve o LabChip® GX Touch, uma plataforma de alta tecnologia que integra a praticidade de um sistema de simples operação ao toque dos dedos. O sistema permite realizar análises de eletroforese em chip em questão de segundos, substituindo a tradicional eletroforese em gel. O usuário prepara rapidamente a amostra no chip e obtém resultados qualitativos e quantitativos de DNA ou RNA de forma automática e precisa.
Analysis of Trace Elements in Fertilizer with AVIO 200 ICP-OESPerkinElmer, Inc.
This document summarizes the ability of the Avio 200 ICP-OES to accurately analyze trace elements in fertilizers at levels below Thailand's regulatory limits. Reference materials were digested and analyzed to validate the method. Results for the reference materials showed recoveries within 10% of certified values. Detection limits for the analytes were all below the corresponding regulatory levels in Thailand. The method demonstrated the Avio 200 ICP-OES can accurately measure nutrients and trace elements in fertilizers.
Analysis of Impurities in Semiconductor-Grade Hydrochloric Acid with the NexI...PerkinElmer, Inc.
This document discusses the analysis of impurities in semiconductor-grade hydrochloric acid (HCl) using inductively coupled plasma mass spectrometry (ICP-MS). HCl is commonly used to clean silicon wafers during semiconductor production, and increasingly stringent purity standards are required. ICP-MS provides the necessary low detection limits for trace metal analysis down to parts-per-trillion levels. However, polyatomic interferences from argon, oxygen, and hydrogen ions must be addressed. The PerkinElmer NexION 2000 ICP-MS uses reaction, cool plasma, and quadrupole modes to remove interferences and achieve detection limits below 1 ng/L for most elements, allowing accurate analysis of impurities in
New Research Evaluating Cisplatin Uptake in Ovarian Cancer Cells by Single Ce...PerkinElmer, Inc.
Application Note
Authors:
Lauren Amable
National Institute on Minority Health and
Health Disparities, National Institutes of Health
Bethesda, MD
Stan Smith
Chady Stephan
PerkinElmer, Inc.
Shelton, CT
O documento descreve o cromatógrafo gasoso PerkinElmer Clarus All-In-One, que possui um gerador de gases hidrogênio e ar sintético integrado, eliminando a necessidade de instalação de linhas de gases caras ou centrais de armazenamento de cilindros. O sistema possui manutenção simplificada, tela sensível ao toque, regeneração automática do secador a frio e modo stand-by automático controlado por sensor de hidrogênio.
High Quality DNA Isolation Suitable for Ultra Rapid SequencingPerkinElmer, Inc.
1) Modifications were made to decrease the time for whole genome sequencing (WGS) including faster DNA isolation, optimized sample preparation, sequencing and bioinformatics analysis.
2) DNA isolation using the chemagic MSM I instrument was optimized to isolate DNA from 24 blood samples in 1.5 hours, yielding 40 μg/mL of high quality DNA suitable for sequencing.
3) Sequencing on an Illumina HiSeq 2500 was modified for ultra rapid runs, taking approximately 20 hours to sequence a genome to 40x coverage while maintaining over 90% of bases above Q30. Alignment and variant detection using Isaac software took less than 3.5 hours.
How information systems are built or acquired puts information, which is what they should be about, in a secondary place. Our language adapted accordingly, and we no longer talk about information systems but applications. Applications evolved in a way to break data into diverse fragments, tightly coupled with applications and expensive to integrate. The result is technical debt, which is re-paid by taking even bigger "loans", resulting in an ever-increasing technical debt. Software engineering and procurement practices work in sync with market forces to maintain this trend. This talk demonstrates how natural this situation is. The question is: can something be done to reverse the trend?
Monitoring and Managing Anomaly Detection on OpenShift.pdfTosin Akinosho
Monitoring and Managing Anomaly Detection on OpenShift
Overview
Dive into the world of anomaly detection on edge devices with our comprehensive hands-on tutorial. This SlideShare presentation will guide you through the entire process, from data collection and model training to edge deployment and real-time monitoring. Perfect for those looking to implement robust anomaly detection systems on resource-constrained IoT/edge devices.
Key Topics Covered
1. Introduction to Anomaly Detection
- Understand the fundamentals of anomaly detection and its importance in identifying unusual behavior or failures in systems.
2. Understanding Edge (IoT)
- Learn about edge computing and IoT, and how they enable real-time data processing and decision-making at the source.
3. What is ArgoCD?
- Discover ArgoCD, a declarative, GitOps continuous delivery tool for Kubernetes, and its role in deploying applications on edge devices.
4. Deployment Using ArgoCD for Edge Devices
- Step-by-step guide on deploying anomaly detection models on edge devices using ArgoCD.
5. Introduction to Apache Kafka and S3
- Explore Apache Kafka for real-time data streaming and Amazon S3 for scalable storage solutions.
6. Viewing Kafka Messages in the Data Lake
- Learn how to view and analyze Kafka messages stored in a data lake for better insights.
7. What is Prometheus?
- Get to know Prometheus, an open-source monitoring and alerting toolkit, and its application in monitoring edge devices.
8. Monitoring Application Metrics with Prometheus
- Detailed instructions on setting up Prometheus to monitor the performance and health of your anomaly detection system.
9. What is Camel K?
- Introduction to Camel K, a lightweight integration framework built on Apache Camel, designed for Kubernetes.
10. Configuring Camel K Integrations for Data Pipelines
- Learn how to configure Camel K for seamless data pipeline integrations in your anomaly detection workflow.
11. What is a Jupyter Notebook?
- Overview of Jupyter Notebooks, an open-source web application for creating and sharing documents with live code, equations, visualizations, and narrative text.
12. Jupyter Notebooks with Code Examples
- Hands-on examples and code snippets in Jupyter Notebooks to help you implement and test anomaly detection models.
Your One-Stop Shop for Python Success: Top 10 US Python Development Providersakankshawande
Simplify your search for a reliable Python development partner! This list presents the top 10 trusted US providers offering comprehensive Python development services, ensuring your project's success from conception to completion.
Freshworks Rethinks NoSQL for Rapid Scaling & Cost-EfficiencyScyllaDB
Freshworks creates AI-boosted business software that helps employees work more efficiently and effectively. Managing data across multiple RDBMS and NoSQL databases was already a challenge at their current scale. To prepare for 10X growth, they knew it was time to rethink their database strategy. Learn how they architected a solution that would simplify scaling while keeping costs under control.
In the realm of cybersecurity, offensive security practices act as a critical shield. By simulating real-world attacks in a controlled environment, these techniques expose vulnerabilities before malicious actors can exploit them. This proactive approach allows manufacturers to identify and fix weaknesses, significantly enhancing system security.
This presentation delves into the development of a system designed to mimic Galileo's Open Service signal using software-defined radio (SDR) technology. We'll begin with a foundational overview of both Global Navigation Satellite Systems (GNSS) and the intricacies of digital signal processing.
The presentation culminates in a live demonstration. We'll showcase the manipulation of Galileo's Open Service pilot signal, simulating an attack on various software and hardware systems. This practical demonstration serves to highlight the potential consequences of unaddressed vulnerabilities, emphasizing the importance of offensive security practices in safeguarding critical infrastructure.
Must Know Postgres Extension for DBA and Developer during MigrationMydbops
Mydbops Opensource Database Meetup 16
Topic: Must-Know PostgreSQL Extensions for Developers and DBAs During Migration
Speaker: Deepak Mahto, Founder of DataCloudGaze Consulting
Date & Time: 8th June | 10 AM - 1 PM IST
Venue: Bangalore International Centre, Bangalore
Abstract: Discover how PostgreSQL extensions can be your secret weapon! This talk explores how key extensions enhance database capabilities and streamline the migration process for users moving from other relational databases like Oracle.
Key Takeaways:
* Learn about crucial extensions like oracle_fdw, pgtt, and pg_audit that ease migration complexities.
* Gain valuable strategies for implementing these extensions in PostgreSQL to achieve license freedom.
* Discover how these key extensions can empower both developers and DBAs during the migration process.
* Don't miss this chance to gain practical knowledge from an industry expert and stay updated on the latest open-source database trends.
Mydbops Managed Services specializes in taking the pain out of database management while optimizing performance. Since 2015, we have been providing top-notch support and assistance for the top three open-source databases: MySQL, MongoDB, and PostgreSQL.
Our team offers a wide range of services, including assistance, support, consulting, 24/7 operations, and expertise in all relevant technologies. We help organizations improve their database's performance, scalability, efficiency, and availability.
Contact us: info@mydbops.com
Visit: https://www.mydbops.com/
Follow us on LinkedIn: https://in.linkedin.com/company/mydbops
For more details and updates, please follow up the below links.
Meetup Page : https://www.meetup.com/mydbops-databa...
Twitter: https://twitter.com/mydbopsofficial
Blogs: https://www.mydbops.com/blog/
Facebook(Meta): https://www.facebook.com/mydbops/
5th LF Energy Power Grid Model Meet-up SlidesDanBrown980551
5th Power Grid Model Meet-up
It is with great pleasure that we extend to you an invitation to the 5th Power Grid Model Meet-up, scheduled for 6th June 2024. This event will adopt a hybrid format, allowing participants to join us either through an online Mircosoft Teams session or in person at TU/e located at Den Dolech 2, Eindhoven, Netherlands. The meet-up will be hosted by Eindhoven University of Technology (TU/e), a research university specializing in engineering science & technology.
Power Grid Model
The global energy transition is placing new and unprecedented demands on Distribution System Operators (DSOs). Alongside upgrades to grid capacity, processes such as digitization, capacity optimization, and congestion management are becoming vital for delivering reliable services.
Power Grid Model is an open source project from Linux Foundation Energy and provides a calculation engine that is increasingly essential for DSOs. It offers a standards-based foundation enabling real-time power systems analysis, simulations of electrical power grids, and sophisticated what-if analysis. In addition, it enables in-depth studies and analysis of the electrical power grid’s behavior and performance. This comprehensive model incorporates essential factors such as power generation capacity, electrical losses, voltage levels, power flows, and system stability.
Power Grid Model is currently being applied in a wide variety of use cases, including grid planning, expansion, reliability, and congestion studies. It can also help in analyzing the impact of renewable energy integration, assessing the effects of disturbances or faults, and developing strategies for grid control and optimization.
What to expect
For the upcoming meetup we are organizing, we have an exciting lineup of activities planned:
-Insightful presentations covering two practical applications of the Power Grid Model.
-An update on the latest advancements in Power Grid -Model technology during the first and second quarters of 2024.
-An interactive brainstorming session to discuss and propose new feature requests.
-An opportunity to connect with fellow Power Grid Model enthusiasts and users.
Essentials of Automations: Exploring Attributes & Automation ParametersSafe Software
Building automations in FME Flow can save time, money, and help businesses scale by eliminating data silos and providing data to stakeholders in real-time. One essential component to orchestrating complex automations is the use of attributes & automation parameters (both formerly known as “keys”). In fact, it’s unlikely you’ll ever build an Automation without using these components, but what exactly are they?
Attributes & automation parameters enable the automation author to pass data values from one automation component to the next. During this webinar, our FME Flow Specialists will cover leveraging the three types of these output attributes & parameters in FME Flow: Event, Custom, and Automation. As a bonus, they’ll also be making use of the Split-Merge Block functionality.
You’ll leave this webinar with a better understanding of how to maximize the potential of automations by making use of attributes & automation parameters, with the ultimate goal of setting your enterprise integration workflows up on autopilot.
Have you ever been confused by the myriad of choices offered by AWS for hosting a website or an API?
Lambda, Elastic Beanstalk, Lightsail, Amplify, S3 (and more!) can each host websites + APIs. But which one should we choose?
Which one is cheapest? Which one is fastest? Which one will scale to meet our needs?
Join me in this session as we dive into each AWS hosting service to determine which one is best for your scenario and explain why!
Fueling AI with Great Data with Airbyte WebinarZilliz
This talk will focus on how to collect data from a variety of sources, leveraging this data for RAG and other GenAI use cases, and finally charting your course to productionalization.
zkStudyClub - LatticeFold: A Lattice-based Folding Scheme and its Application...Alex Pruden
Folding is a recent technique for building efficient recursive SNARKs. Several elegant folding protocols have been proposed, such as Nova, Supernova, Hypernova, Protostar, and others. However, all of them rely on an additively homomorphic commitment scheme based on discrete log, and are therefore not post-quantum secure. In this work we present LatticeFold, the first lattice-based folding protocol based on the Module SIS problem. This folding protocol naturally leads to an efficient recursive lattice-based SNARK and an efficient PCD scheme. LatticeFold supports folding low-degree relations, such as R1CS, as well as high-degree relations, such as CCS. The key challenge is to construct a secure folding protocol that works with the Ajtai commitment scheme. The difficulty, is ensuring that extracted witnesses are low norm through many rounds of folding. We present a novel technique using the sumcheck protocol to ensure that extracted witnesses are always low norm no matter how many rounds of folding are used. Our evaluation of the final proof system suggests that it is as performant as Hypernova, while providing post-quantum security.
Paper Link: https://eprint.iacr.org/2024/257
LF Energy Webinar: Carbon Data Specifications: Mechanisms to Improve Data Acc...DanBrown980551
This LF Energy webinar took place June 20, 2024. It featured:
-Alex Thornton, LF Energy
-Hallie Cramer, Google
-Daniel Roesler, UtilityAPI
-Henry Richardson, WattTime
In response to the urgency and scale required to effectively address climate change, open source solutions offer significant potential for driving innovation and progress. Currently, there is a growing demand for standardization and interoperability in energy data and modeling. Open source standards and specifications within the energy sector can also alleviate challenges associated with data fragmentation, transparency, and accessibility. At the same time, it is crucial to consider privacy and security concerns throughout the development of open source platforms.
This webinar will delve into the motivations behind establishing LF Energy’s Carbon Data Specification Consortium. It will provide an overview of the draft specifications and the ongoing progress made by the respective working groups.
Three primary specifications will be discussed:
-Discovery and client registration, emphasizing transparent processes and secure and private access
-Customer data, centering around customer tariffs, bills, energy usage, and full consumption disclosure
-Power systems data, focusing on grid data, inclusive of transmission and distribution networks, generation, intergrid power flows, and market settlement data
Session 1 - Intro to Robotic Process Automation.pdfUiPathCommunity
👉 Check out our full 'Africa Series - Automation Student Developers (EN)' page to register for the full program:
https://bit.ly/Automation_Student_Kickstart
In this session, we shall introduce you to the world of automation, the UiPath Platform, and guide you on how to install and setup UiPath Studio on your Windows PC.
📕 Detailed agenda:
What is RPA? Benefits of RPA?
RPA Applications
The UiPath End-to-End Automation Platform
UiPath Studio CE Installation and Setup
💻 Extra training through UiPath Academy:
Introduction to Automation
UiPath Business Automation Platform
Explore automation development with UiPath Studio
👉 Register here for our upcoming Session 2 on June 20: Introduction to UiPath Studio Fundamentals: https://community.uipath.com/events/details/uipath-lagos-presents-session-2-introduction-to-uipath-studio-fundamentals/
What is an RPA CoE? Session 1 – CoE VisionDianaGray10
In the first session, we will review the organization's vision and how this has an impact on the COE Structure.
Topics covered:
• The role of a steering committee
• How do the organization’s priorities determine CoE Structure?
Speaker:
Chris Bolin, Senior Intelligent Automation Architect Anika Systems
Dandelion Hashtable: beyond billion requests per second on a commodity serverAntonios Katsarakis
This slide deck presents DLHT, a concurrent in-memory hashtable. Despite efforts to optimize hashtables, that go as far as sacrificing core functionality, state-of-the-art designs still incur multiple memory accesses per request and block request processing in three cases. First, most hashtables block while waiting for data to be retrieved from memory. Second, open-addressing designs, which represent the current state-of-the-art, either cannot free index slots on deletes or must block all requests to do so. Third, index resizes block every request until all objects are copied to the new index. Defying folklore wisdom, DLHT forgoes open-addressing and adopts a fully-featured and memory-aware closed-addressing design based on bounded cache-line-chaining. This design offers lock-free index operations and deletes that free slots instantly, (2) completes most requests with a single memory access, (3) utilizes software prefetching to hide memory latencies, and (4) employs a novel non-blocking and parallel resizing. In a commodity server and a memory-resident workload, DLHT surpasses 1.6B requests per second and provides 3.5x (12x) the throughput of the state-of-the-art closed-addressing (open-addressing) resizable hashtable on Gets (Deletes).
1. CONTENTS
TABLE OF
SPOTLIGHT
ON APPLICATIONS.
FOR A BETTER
TOMORROW.
SPECIAL EDITION – FOOD & BEVERAGE
VOLUME 1
2. CONTENTS
TABLE OF
INTRODUCTION
PerkinElmer Spotlight on Applications e-Zine –
Food & Beverage Special Edition
Welcome to our Spotlight on Applications e-zine. Whether you’ve experienced
Spotlight on Applications in the past or are hearing about it for the first time, we
want to share with you this special edition, dedicated to recent application releases
within Food & Beverage.
Spotlight on Applications is a quarterly e-zine compendium, delivering a variety
of topics that address the pressing issues and analytical challenges you may face
in your application areas today.
This Special Edition features a broad range of applications within Food Safety,
Quality/Conformance/Authenticity, as well as Nutrition/Labeling, which you will
be able to access at your convenience. Each application in the table of contents
includes an embedded link which takes you directly to the appropriate page
within the e-zine.
We invite you to explore, enjoy and learn!
And don’t miss out – if you subscribe, you will automatically receive new volumes
as they are released.
Be sure to receive future
issues by subscribing here.
PerkinElmer
3. CONTENTS
TABLE OF
CONTENTS
Food Safety (Contaminants, Toxins, Pesticides, Antibiotics)
• Determination of Toxic, Essential, and Nutritional Elements in Food Matrices Using an ICP-MS
• etermination of Toxic, Trace, Essential Elements in Food Matrices using THGA Coupled with Longitudinal Zeeman Background Correction
D
• nalysis of Pb, Cd and As in Spice Mixtures using Graphite Furnace Atomic Absorption
A
• oxic Trace Metals in Edible Oils by Graphite Furnace Atomic Absorption
T
• nalysis of Pb, Cd and As in Tea Leaves Using Graphite Furnace Atomic Absorption
A
• ccurate Determination of Lead in Different Dairy Products by Graphite Furnace Atomic Absorption
A
• etermination of Low Levels of Benzene, Toluene, Ethylbenzene, Xylenes and Styrene in Olive Oil Using TurboMatrix HS and
D
Clarus SQ 8 GC/MS
• etermination of Furan in Food by GC/MS and Headspace Sampling
D
• ungicide on Lemon Peel Using Direct Sample Analysis TOF Mass Spectrometry
F
• ungicides on Oranges Using Direct Sample Analysis TOF Mass Spectrometry
F
• alathion on Peaches Using Direct Sample Analysis TOF Mass Spectrometry
M
Quality/Conformance/Authenticity
• onitoring VOCs in Beer Production Using the Clarus SQ 8 GC/MS and TurboMatrix Headspace Trap Systems
M
• ualitative Characterization of Fruit Juice Flavor Using a TurboMatrix HS Trap and a Clarus SQ 8 GC/MS
Q
• ualifying Mustard Flavor by Headspace Trap GC/MS using the Clarus SQ 8
Q
• easurement of Quality of Crude Palm Oils Used in Margarine Production by UV/Vis Spectroscopy
M
• ractical Food Applications of Differential Scanning Calorimetry
P
• apsaicin and Dihydrocapsaicin in Peppers Using Direct Sample Analysis TOF Mass Spectrometry
C
• urcumins in Turmeric Powder Using Direct Sample Analysis TOF Mass Spectrometry
C
• esveratol in Red Wine Using Direct Sample Analysis TOF Mass Spectrometry
R
Nutrition/Labeling
• Quantification of Essential Metals in Spice Mixtures for Regulatory Compliance Using Flame Atomic Absorption
• etermination of Toxic, Essential, and Nutritional Elements in Food Matrices Using an ICP-MS
D
• etermination of Toxic, Trace, Essential Elements in Food Matrices using THGA Coupled with Longitudinal Zeeman Background Correction
D
• nalysis of Common Antioxidants in Edible Oil with Flexar FX-15 and PDA
A
• terols in Olive Oil Using Direct Sample Analysis TOF Mass Spectrometry
S
• nalysis of the Mycotoxin Patulin in Apple Juice Using the Flexar FX-15 UHPLC-UV
A
• imultaneous Analysis of Nine Food Additives with the PerkinElmer Flexar FX-15 System Equipped with a PDA Detector
S
• weeteners in Diet Cola Using Direct Sample Analysis TOF Mass Spectrometry
S
• elatonin in Lazy Cake® Using Direct Sample Analysis TOF Mass Spectrometry
M
PerkinElmer
4. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
ICP – Mass Spectrometry
Authors:
Cynthia Bosnak
Senior Product Specialist
Ewa Pruszkowski
Senior Product Specialist
Food Safety
PerkinElmer, Inc.
Shelton, CT USA
Quality/Conformance/
The Determination of Introduction
The elemental and dynamic range of inductively
Authenticity
Toxic, Essential, and coupled plasma-mass spectrometry (ICP-MS) makes
it ideally suited for the analysis of food materials.
Nutritional Elements The ultratrace detection limits of ICP-MS permit
the determination of low-level contaminants such
in Food Matrices as Pb, As, Se, and Hg, while the macro-level
nutritional elements such as Ca, Mg, K, and Na
Using an ICP-MS can be quantified using the extended dynamic
range capability of ICP-MS which provides 9-orders
of magnitude. However, there are still a number
of challenges to overcome, which makes the routine analysis of foods difficult
unless the sample dissolution procedure is well thought out and instrumental
conditions are optimized for complex sample matrices.
Nutrition/Labeling
Download Entire Application Note
5. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Atomic Absorption
Authors
David Bass
Senior Product Specialist
Cynthia P. Bosnak
Senior Product Specialist
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
The Determination of Toxic, Introduction
Ingestion of trace elements from food
Trace, and Essential Elements in
Quality/Conformance/
can be linked to nutrition, disease, and
physiological development. Whether they
Food Matrices using THGA are needed for proper nutritional value or
Authenticity
contain toxic elements, the presence of
Coupled with Longitudinal major and minor elements in food needs
to be verified to help determine health
Zeeman Background Correction effects for the consumer. Contamination
of food products may result from metals
present during cultivation and/or processing.
Acute or chronic exposure to heavy metals
can lead to damaged nervous system function and have detrimental effects on vital
organs. Food safety laboratories performing these analyses are often high-throughput
facilities and require a detection tool that is efficient and cost effective.
Unlike flame atomic absorption spectrophotometry (FAAS) where the ground
state atoms quickly diffuse into surrounding air, graphite furnace atomic absorption
spectrophotometry (GFAAS), being a total consumption technique, offers the
ability to dry and atomize the entire pipetted sample in a more controlled
environment within the graphite tube. This significantly increases sensitivity and Nutrition/Labeling
provides superior detection limits with microliter (µL) sample volumes. Only
ICP-MS can provide the same level of detection as GFAAS, however GFAAS is more
cost efficient, simpler to operate and has fewer laboratory facility requirements.
Download Entire Application Note
6. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Atomic Absorption
Author
Praveen Sarojam, Ph.D.
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Analysis of Pb, Cd and Introduction
The toxicity and effect of trace heavy metals on
As in Spice Mixtures human health and the environment has attracted
considerable attention and concern in recent years.
using Graphite Furnace With an inherent toxicity, a tendency to accumulate
in the food chain and a particularly low removal rate
Atomic Absorption
Quality/Conformance/
through excretion,1 lead (Pb), cadmium (Cd) and
arsenic (As) cause harm to humans even at low
Spectrophotometry concentrations. Exposure to trace and heavy metals
Authenticity
above the permissible level affects human health and
may result in teratogenicity (reproductive effects).
Individuals may also experience high blood pressure,
fatigue, as well as kidney and neurological disorders.
Spices, the dried parts of plants, grow widely in various regions of the world, are produced
either on small farmlands or naturally grown, and have been used for several purposes since
ancient times. Most are fragrant and flavorful and are used for culinary purposes to improve
the quality of food.2 Natural food spices, such as pepper, have been reported to contain
significant quantities of some heavy metals, including Pb, Cd and As. Contamination with
heavy metals may be accidental (e.g. contamination of the environment during plant
cultivation) or deliberate – in some cultures, according to traditional belief, specially treated
heavy metals are associated with health benefits and are thus an intentional ingredient of
traditional remedies. Spices and herbal plants may contain heavy metal ions over a wide
range of concentrations.3,4 There is often little information available about the safety of
those plants and their products in respect to heavy metal contamination. Due to the
Nutrition/Labeling
significant amount of spices consumed, it is important to know the toxic metal
concentrations in them.5
Download Entire Application Note
7. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Atomic Absorption
Authors
Surasak Manarattanasuwan
Senior Inorganic Product Specialist
PerkinElmer, Inc.
Thailand
Food Safety
Toxic Trace Metals Introduction
Graphite furnace atomic absorption spectropho-
in Edible Oils by tometry (GFAAS) has been widely applied to the
determination of trace elements in food due to
Graphite Furnace its selectivity, simplicity, high sensitivity, and its
Quality/Conformance/
capability for accurate determinations in a wide
Atomic Absorption variety of matrices. Edible oils are generally
Authenticity
low in trace element concentrations, however,
Spectrophotometry metals such as arsenic (As), lead (Pb), cadmium
(Cd), chromium (Cr), and selenium (Se) can be
found and are known for their toxicities which
affect the health of consumers. The determination
of toxic elements from naturally occurring or production-contamination sources
in oils can be determined by using GFAAS or inductively coupled plasma mass
spectrometry (ICP-MS). When only a few elements are being analyzed, GFAAS is
the preferred choice. It is easy to learn, faster in setting up, and simpler to use
than ICP-MS. GFAAS is also lower in initial capital investment and has a lower
operating and maintenance cost. Sample pretreatment procedures for edible
oils are normally required prior to instrumental analysis in order to eliminate the
organic matrix. Wet, dry or microwave digestion, dilution with organic solvent,
and extraction methods can be time consuming and require more operator training
than a direct analysis method.
Nutrition/Labeling
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8. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Atomic Absorption
Author
Praveen Sarojam, Ph.D.
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Analysis of Pb, Cd and As in Introduction
Tea is drunk by about half of the world’s
Tea Leaves Using Graphite population. It is widely cultivated and
consumed in Southeast Asia. Tea is rich
Furnace Atomic Absorption in many trace inorganic elements.1,2
In addition to many essential elements
Spectrophotometry required for human health, some toxic
Quality/Conformance/
elements may also be present in tea
leaves. This could be due to polluted soil,
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application of pesticides, fertilizers or industrial activities. There is often little information
available about the safety of tea leaves and finished tea products with respect to heavy
metal contamination. Due to the significant amount of tea consumed, it is important to
know the toxic metal contents.
The toxicity and effect of trace heavy metals on human health and the environment has
attracted considerable attention and concern in recent years. Among the heavy metals, lead
(Pb), cadmium (Cd) and arsenic (As) are especially toxic and are harmful to humans even
at low concentrations. They have an inherent toxicity with a tendency to accumulate in the
food chain and a particularly low removal rate through excretion.3 Exposure to heavy metals
above the permissible level can cause high blood pressure, fatigue, as well as kidney and
neurological disorders. Heavy metals are also known to cause harmful reproductive effects.4
A major challenge in the analysis of tea leaves is the extremely low analyte levels and the
very high matrix levels. For many years, graphite furnace atomic absorption spectrophotometry
(GFAAS) has been a reliable technique and the preferred method for this analysis. The use of
Nutrition/Labeling
longitudinal Zeeman background correction and matrix modifiers help to achieve extremely
low detection limits in high matrix samples such as tea leaves, making GFAAS an indispensible
tool for carrying out such analyses.
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9. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Atomic Absorption
Authors
Jijun Yao
Renkang Yang
Jianmin Chen
PerkinElmer, Inc.
Food Safety
Shelton, CT 06484 USA
Accurate Determination Introduction
Milk is one of the basic food groups in the human
of Lead in Different diet, both in its original form and as various dairy
products. The Chinese contaminated baby formula
Dairy Products by scandal in 2008 has increased public awareness of
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contamination possibilities, and has lead to tighter
Graphite Furnace Atomic supervision of dairy products as China is faced with
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demands – both from home and abroad – to improve
Absorption Spectrometry its food safety record. It is well-known that lead (Pb)
is toxic and causes damage to the nervous system; it
has a particularly detrimental effect on young chil-
dren1 and it has become a cause of major concern since the 1970s. As per World Health
Organization (WHO) standards, the permissible limit of lead in drinking water is 10 µg/kg
(parts per billion, ppb). Following an in-depth review of the toxicological literature, the
Chinese guideline for maximum levels of lead content is set at 20 µg/kg (ppb wet weight) in
infant formula (use of milk as a raw material measured by fluid milk diluted from powder,
referring to the product ready-to-use) and at 50 µg/kg (ppb) in fresh milk, respectively.2
Lead analysis has traditionally been one of the major applications of graphite furnace atomic
absorption spectrometry (GFAAS) worldwide. Currently, the Chinese regulatory framework
approved standard methods for lead analysis has set GFAAS as the technique for the
compulsory arbitration in food testing.3 In order to ensure protection of consumers, analysis
should be sensitive, efficient, and cost-effective so that more effective monitoring can be
accomplished. Because GFAAS is a mature technique, it is well-understood and routinely Nutrition/Labeling
used by technicians and suitable for this determination. Sample preparation is an important
part of an analysis and yet can be time consuming.
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10. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Gas Chromatography/
Mass Spectrometry
Author
A. Tipler, Senior Scientist
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
The Determination of Low Levels of Benzene,
Toluene, Ethylbenzene, Xylenes and Styrene in
Olive Oil Using a Turbomatrix HS and a Clarus
Quality/Conformance/
SQ 8 GC/MS
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Introduction
Levels of benzene, toluene, ethylbenzene, xylenes and styrene (BTEXS) are a concern in olive
oil. These compounds find their way into olive trees and hence into the olives and olive oil
mainly as a result of emissions from vehicles, bonfires, and paints into ambient air near the
orchards.
Various methods have been developed to detect and quantify these compounds down to
levels of 5 ng/g (5 ppb w/w). This application note describes an easy to perform method
using PerkinElmer® Clarus® SQ 8 GC/MS with a TurboMatrix™ 110 headspace sampler to
achieve detection limits below 0.5 ng/g.
Nutrition/Labeling
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11. CONTENTS
TABLE OF
APPLICATION NOTe
GC-Mass Spectrometry
and Headspace Sampling
Author
Padmaja Prabhu
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Determination of Introduction
Furan is naturally occurring at low levels in many foods
Furan in Food by and drinks.1 Furan consumption is of concern because
it has been classified by the International Agency for
Gas Chromatography- Research on Cancer (IARC) as possibly carcinogenic to
humans, based on studies with laboratory animals. The
Mass Spectrometry and
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U.S. FDA has recently published a report on the occur-
Headspace Sampling rence of furan in a large number of thermally processed
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foods, especially canned and jarred foods, including
baby foods and infant formulas. The primary source of
furan in food is considered to be thermal degradation
of carbohydrates, such as glucose, lactose and fructose.
Of all the foods tested in various papers, coffee contained the largest amount of furans.1 Furan is a
colorless, volatile and lipophilic organic compound. It has a molecular weight of 68 and a low boiling
point (31 ˚C). Due to its high volatility, furan levels in foods are easily determined, with high accuracy,
by headspace methods.
This application note will demonstrate a rapid method for the identification and quantification of
furan in food samples, using gas chromatography with headspace sampling and mass spectrometry.
In addition to method optimization and standard analysis, we will analyze a number of food samples
for furan. We chose to test coffee containing drinks, sauces, and canned foods, as previous studies
demonstrated high levels of furan in these foods. The samples were randomly collected from the
local market.
Nutrition/Labeling
Figure 1. Structure and physical properties of furan.
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15. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Gas Chromatography/
Mass Spectrometry
Authors
Lee Marotta
Sr. Field Application Scientist
Andrew Tipler
Senior Scientist
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Monitoring Volatile Introduction
Beer is a popular beverage produced by the fermentation
Organic Compounds of hopped malt extracted from barley and other
grains. Although simple in concept, beer is a highly
in Beer Production Using complex mixture of many compounds including
the Clarus SQ 8 GC/MS sugars, proteins, alcohols, esters, acids, ketones, acids
Quality/Conformance/
and terpenes. Flavor is an important quality of any
and TurboMatrix Headspace beer and the chemical content of the beer is obviously
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responsible for that flavor. Aroma is an extremely
Trap Systems important part of the flavor and so there is a strong
interest by brewers in the volatile organic compounds
(VOCs) in beer that affect its aroma.
Some VOCs have a positive effect on aroma (attributes) and some have a negative
effect (defects). The ability to characterize these in beer products before, during and
after fermentation would be an important tool in process control, quality assurance
and product development.
This application note describes a system comprising a headspace trap sampler to extract
and concentrate VOCs from a beer sample and deliver them to a gas chromatograph/
mass spectrometer (GC/MS) for separation, identification and quantification.
The purpose of our experiments is to demonstrate that attributes and defects can all be
monitored using one detector and from a single injection with mass spectrometry (MS).
The associated benefits include a quicker return on investment, enhanced productivity, Nutrition/Labeling
more information from a single analysis, and less bench space requirements.
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16. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Gas Chromatography/
Mass Spectroscopy
Author
A. Tipler, Senior Scientist
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
The Qualitative Characterization
of Fruit Juice Flavor using a
TurboMatrix HS Trap and
a Clarus SQ 8 GC/MS
Quality/Conformance/
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Introduction
The PerkinElmer® TurboMatrix™ Headspace Trap system coupled with a Clarus®
SQ 8 GC/MS is a very convenient means of identifying low concentration volatile
organic compounds (VOCs) in foodstuffs. In this application note, the VOCs in
various fruit juices were investigated. Sample preparation simply involved dispensing
a fixed volume of fruit juice into a sample vial and sealing it. The analysis was
fully automated.
Nutrition/Labeling
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17. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Gas Chromatography/
Mass Spectrometry
Author
Ruben Garnica
Andrew Tipler
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Quality/Conformance/
Qualifying Mustard Mustard is a common condiment used across many cultures
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and culinary styles to enhance the dining experience. It
Flavor by Headspace is derived from the mustard seed and is used either as a
dried spice, spread or paste when the dried spice is mixed
Trap GC/MS using with water, vinegar or other liquid. The characteristic
sharp taste of mustard arises from the isothiocyanates
the Clarus SQ 8 (ITCs) present as result of enzymatic activity made possible
when the ground seed is mixed with liquids. The focus of
this application brief is the characterization of these ITCs
by headspace trap gas chromatography/mass spectrometry
(GC/MS) and a qualitative description of their relationship
to sharpness in taste across various mustard products.
Nutrition/Labeling
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18. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
UV/Vis Spectroscopy
Author
Steve Upstone
PerkinElmer, Inc.
Seer Green, UK
Food Safety
Measurement of
Quality/Conformance/
Crude Palm Oil (CPO) is a raw material used in the
production of margarine and other vegetable oil based food
Quality of Crude products. CPO is traded and there are quality specifications
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based on free fatty acids (FFAs) as well as moisture and
Palm Oils used in impurities.1,2,3
Margarine Production Margarine manufacturers also want to assess the CPO’s
‘fitness for refining’ which is measured by the Deterioration
by UV/Visible of Bleachability Index (DOBI). A DOBI index of less than
1.8 indicates a poor quality oil; a DOBI index 3 indicates
Spectroscopy a high quality oil
The DOBI index is defined as the absorbance ratio A446 nm /
A269 nm of around 0.04 g oil dissolved in 25 mL of hexane
or 2,2,4Trimethylpentane (iso-octane).
Rather than simply measuring the DOBI at fixed wavelengths, there are advantages in measuring
the spectrum between 220 and 500 nm as it means that it is also possible to calculate the carotene
content by measuring the CPOs primary and secondary oxidation products. In addition, any adulterants
Nutrition/Labeling
added to enhance the DOBI can be detected by examining the spectrum in more detail.
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19. CONTENTS
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a p p l i c at i o n n o t e
Differential Scanning Calorimetry
Authors
Patricia Heussen
Unilever
Research Development
Vlaardingen, The Netherlands
Peng Ye, Kevin Menard, Patrick Courtney
Food Safety
PerkinElmer, Inc.
Shelton, CT 06484 USA
Practical Food Applications of
Differential Scanning Calorimetry (DSC)
Abstract
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This note describes a number of important food applications utilising the PerkinElmer DSC demonstrating
the versatility of the technique as a tool in the food industry.
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Introduction
Food is often a complex system including various compositions and structures. The characterization
of food can therefore be challenging. Many analytical methods have been used to study food,
including differential scanning calorimetry (DSC).1 DSC is a thermal analysis technique to measure
the temperature and heat flows associated with phase transitions in materials, as a function of
time and temperature. Such measurements can provide both quantitative and qualitative informa-
tion concerning physical and chemical changes that involve endothermic (energy consuming) and
exothermic (energy producing) processes, or changes in heat capacity.
DSC is particularly suitable for analysis of food systems because they are often subject to heating
or cooling during processing. The calorimetric information from DSC can be directly used to under-
stand the thermal transitions that the food system may undergo during processing or storage. DSC
is easy to operate and in most cases no special sample preparation is required. With a wide range
of DSC sample pans available, both liquid and solid food samples can be studied. Typical food
samples and the type of information that can be obtained by DSC are listed in Table 1. These tests Nutrition/Labeling
can be used for both QC and RD purposes. DSC applications are used from troubleshooting up to
new product developments.
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23. CONTENTS
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a p p l i c at i o n n o t e
Atomic Absorption
Author
Praveen Sarojam, Ph.D.
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Quantification of Introduction
Foods, together with water, provide the major proportion of
Essential Metals the total daily intake of trace elements by humans. Spices
and vegetables are some of the most common foods in the
in Spice Mixtures human diet around the world. Besides polluted soil and
water, foods can also be contaminated with trace metals by
for Regulatory
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the introduction of mechanized farming, the increasing use
of chemicals, food processing and packaging, etc. In order
Compliance Using
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to minimize adverse impact, it is important to measure and
continuously monitor the levels of trace elements in various
Flame Atomic kinds of food materials. Trace element food composition data
are also important for both consumers and health professionals.
Absorption In recent years, food labeling legislation has enforced this
Spectrophotometry requirement. Trace element determination in complex matrices,
such as food, often requires sample preparation prior to
determination by instrumental techniques.1
Cobalt (Co), copper (Cu), manganese (Mn), nickel (Ni) and zinc (Zn) are all essential elements,
not only for mammals, but also for plants. They play important roles in many biological processes
including carbohydrate and lipid metabolism.2 For example, a daily copper intake of 1.5 - 2.0 mg
is essential and copper at nearly 40 ng/mL is required for normal metabolism of many living organisms.3
However, copper at higher levels is toxic to the circulatory system and kidneys. The trace element
content of food items for all the essential elements mentioned above must be controlled on a daily basis.
Nutrition/Labeling
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24. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
ICP – Mass Spectrometry
Authors:
Cynthia Bosnak
Senior Product Specialist
Ewa Pruszkowski
Senior Product Specialist
Food Safety
PerkinElmer, Inc.
Shelton, CT USA
Quality/Conformance/
The Determination of Introduction
The elemental and dynamic range of inductively
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Toxic, Essential, and coupled plasma-mass spectrometry (ICP-MS) makes
it ideally suited for the analysis of food materials.
Nutritional Elements The ultratrace detection limits of ICP-MS permit
the determination of low-level contaminants such
in Food Matrices as Pb, As, Se, and Hg, while the macro-level
nutritional elements such as Ca, Mg, K, and Na
Using an ICP-MS can be quantified using the extended dynamic
range capability of ICP-MS which provides 9-orders
of magnitude. However, there are still a number
of challenges to overcome, which makes the routine analysis of foods difficult
unless the sample dissolution procedure is well thought out and instrumental
conditions are optimized for complex sample matrices.
Nutrition/Labeling
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25. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Atomic Absorption
Authors
David Bass
Senior Product Specialist
Cynthia P. Bosnak
Senior Product Specialist
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
The Determination of Toxic, Introduction
Ingestion of trace elements from food
Trace, and Essential Elements in
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can be linked to nutrition, disease, and
physiological development. Whether they
Food Matrices using THGA are needed for proper nutritional value or
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contain toxic elements, the presence of
Coupled with Longitudinal major and minor elements in food needs
to be verified to help determine health
Zeeman Background Correction effects for the consumer. Contamination
of food products may result from metals
present during cultivation and/or processing.
Acute or chronic exposure to heavy metals
can lead to damaged nervous system function and have detrimental effects on vital
organs. Food safety laboratories performing these analyses are often high-throughput
facilities and require a detection tool that is efficient and cost effective.
Unlike flame atomic absorption spectrophotometry (FAAS) where the ground
state atoms quickly diffuse into surrounding air, graphite furnace atomic absorption
spectrophotometry (GFAAS), being a total consumption technique, offers the
ability to dry and atomize the entire pipetted sample in a more controlled
environment within the graphite tube. This significantly increases sensitivity and Nutrition/Labeling
provides superior detection limits with microliter (µL) sample volumes. Only
ICP-MS can provide the same level of detection as GFAAS, however GFAAS is more
cost efficient, simpler to operate and has fewer laboratory facility requirements.
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26. CONTENTS
TABLE OF
a p p l i c at i o n n o t e
Liquid Chromatography
Author
Njies Pedjie
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Quality/Conformance/
Analysis of Common
Antioxidants in Edible
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Introduction
Oil with the PerkinElmer Phenolic antioxidants and ascorbyl palmitate
(Figure 1, Page 2) are commonly used
Flexar FX-15 System in food to prevent the oxidation of oils.
Oxidized oils cause foul odor and rancidity
Equipped with a PDA in food products. This application note
will present a UHPLC analysis of edible
Detector oils to determine the type and amount of
ten different antioxidants.
The method was developed with a 1.9 µm particle size column to achieve very
high throughput at a low flow rate, reducing solvent consumption. The throughput
of an HPLC method with a 5 µm particle size column will be compared with that
of a UHPLC method with a 1.9 µm particle size column. In addition to throughput
comparisons, method conditions and performance data, including precision and
Nutrition/Labeling
linearity are presented. The results of the method applied to a spiked oil sample
and a sample of vegetable shortening are reported.
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28. CONTENTS
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a p p l i c at i o n n o t e
UHPLC
Author
Padmaja Prabhu
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Analysis of the Mycotoxin Introduction
Patulin is produced by various molds,
Patulin in Apple Juice Using which primarily infect the moldy part of
apples. Removing the moldy and dam-
the Flexar FX-15 UHPLC-UV
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aged parts of the fruit may not eliminate
all the patulin because some of it may
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migrate into sound parts of the flesh.
Also, patulin can be produced within the fruit, even though it may not be visibly
moldy. If moldy apples are used to produce apple juice, the patulin goes into the
juice. It is not destroyed by heat treatments such as the pasteurization process.
Patulin is a natural human toxin and therefore can have genetic affects within
cells, including a developing fetus, the immune system and the nervous system.
The recommended advisory level is 50 µg of patulin/kg in apple juice [50 parts
per billion (ppb)].1 Hydroxymethylfurfural (HMF), also 5-(Hydroxymethyl)furfural,
is an organic compound derived from dehydration of sugars. HMF has been
identified in a wide variety of heat-processed foods including milk, fruit juices,
spirits, honey, etc.2
Nutrition/Labeling
Figure 1. Structure and properties of patulin.
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29. CONTENTS
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a p p l i c at i o n n o t e
UHPLC
Author
Njies Pedjie
PerkinElmer, Inc.
Shelton, CT 06484 USA
Food Safety
Simultaneous Analysis of Introduction
Food additives are natural or synthetic
Nine Food Additives with substances that are added in food,
beverage and pharmaceutical products
the PerkinElmer Flexar for their microbicidal, preservative and
flavoring properties. Among the commonly
FX-15 System Equipped used additives, benzoic acid and its salts
are widely used in beverage and food
with a PDA Detector
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for preservation. Artificial sweeteners
are widely used as sugar substitute in
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calorie-conscious societies, where their
intake provides practically no calories and
also helps fight obesity and its related
ailments.
In most countries, the use of additives is regulated. In the U.S., most additives
are part of the Generally Recognized As Safe (GRAS) ingredients although the
FDA has established Acceptable Daily Intake (ADI) for each of them. There is
a need for analytical techniques to identify and quantify additives because the
food industry is required to list the type and amount of each ingredient on product
labels to help consumers make dietary choices and manage food allergies.
This application note presents a fast and robust liquid chromatography method
to simultaneously test nine widely used additives. Among the additives tested
are: preservatives (benzoic acid, sorbic acid, dehydroacetic acid and methylparaben);
artificial sweeteners (acesulfame potassium, saccharin and aspartame); flavoring
agent (quinine); and a stimulant (caffeine). Method conditions and performance Nutrition/Labeling
data including precision, accuracy and linearity are presented. The method is
applied to a mouthwash and a tonic soda and the type and amount of additives
are confirmed.
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