Peripheral blood smear (PBS)
Blood sf] thin smear agfO{
;s]kl5 air dry u/]/ Romanowsky stain
n] stain u/]/ microscope sf] 10X
objective lens sf] k|of]u u/L k¬/}
smear df x]/]/ ;a{ k|yd micro filaria 5
jf 5}g eg]/ kQf nufpg ;lsG5 .
To; kl5 100x (oil immersion lens)
sf] k|of]u u/L cGo malaria parasite
x? klg kQf nufpg ;lsG5 .
Blood sf] thin smear h;n]
blood df ePsf] parasite x?, RBC
sf] number, shape, size / RBC df
haemoglobin sf] concentration,
WBC sf] number, abnormal WBC
tyf platelets sf] number nfO{ ;
+lÔKt a0f{g ub{5 To:tf] Blood
smear nfO{ peripheral blood
smear elgG5 .
1.RBC
Peripheral blood smear df RBC
sf] shape, size / RBC df
haemoglobin sf] concentration
x]g]{ ul/G5 .
2. WBC
Peripheral Blood smear df
WBC / RBC sf] ;“Vof 1:500 sf]
ratio df /x]sf] x¬G5 .
slxn] sfxL peripheral blood smear
df Band and stabbed form (Horse
shoe shaped stage) eGbf cl3Nnf]
stage sf WBC x? b]lvg] ub{5g To:tf]
cjZyfnfO{ shift to the left eg]/
elgG5 .
Neutrophil x?df ;fwf/0ftof @ b]lv
% j6f lobe x? /x]sf x¬G5g t/ % b]lv
& j6f eGbf a9L lobe x? ePsf
Neutrophil x?nfO{ Hyper segmented
Neutrophil eg]/ elgG5 .
s¬g} s¬g} Neutrophil x?df
toxin sf] sf/0fn] Dark violet
colour sf granule x? kfO{G5g tL
Neutrophil x?nfO{ toxic granules
ePsf Neutrophils (Neutrophils
with toxic granules) elgG5 .
olb peripheral blood smear df
large Lymphocyte eGbf 7Ònf /
dark blue cytoplasm ePsf
Lymphocyte x? b]lvPdf ltlgx?
nfO{ Atypical Lymphocyte elgG5
.
#=Platelets
Peripheral blood smear df WBC /
platelet sf] ;“Vof 1:10 b]lv 30 ;Ddsf]
ratio df /x]sf] x¬G5 .
Platelets eg]sf cell sf ;–;fgf
6¬qmfx? (fragments) x¬g h;sf] size
1-4 micron sf] x¬G5 t/ peripheral
smear df Tof] eGbf 7Ònf] platelet
x? b]lvPdf tL platelet x?nfO{ ghost
cell eg]/ elgG5 .
Smear df platelets clump df jf
separate u/]/ b¬a} form df kfpg
;lsG5 . 100x objective lens n]
x]bf{ Pp6f microscopic field df
slDtdf 15 b]lv 20 j6f platelets
b]lvPdf platelets normal dflgG5 .

Peripheral blood smear

  • 1.
    Peripheral blood smear(PBS) Blood sf] thin smear agfO{ ;s]kl5 air dry u/]/ Romanowsky stain n] stain u/]/ microscope sf] 10X objective lens sf] k|of]u u/L k¬/} smear df x]/]/ ;a{ k|yd micro filaria 5 jf 5}g eg]/ kQf nufpg ;lsG5 .
  • 2.
    To; kl5 100x(oil immersion lens) sf] k|of]u u/L cGo malaria parasite x? klg kQf nufpg ;lsG5 .
  • 3.
    Blood sf] thinsmear h;n] blood df ePsf] parasite x?, RBC sf] number, shape, size / RBC df haemoglobin sf] concentration, WBC sf] number, abnormal WBC tyf platelets sf] number nfO{ ; +lÔKt a0f{g ub{5 To:tf] Blood smear nfO{ peripheral blood smear elgG5 .
  • 4.
    1.RBC Peripheral blood smeardf RBC sf] shape, size / RBC df haemoglobin sf] concentration x]g]{ ul/G5 .
  • 5.
    2. WBC Peripheral Bloodsmear df WBC / RBC sf] ;“Vof 1:500 sf] ratio df /x]sf] x¬G5 .
  • 6.
    slxn] sfxL peripheralblood smear df Band and stabbed form (Horse shoe shaped stage) eGbf cl3Nnf] stage sf WBC x? b]lvg] ub{5g To:tf] cjZyfnfO{ shift to the left eg]/ elgG5 .
  • 7.
    Neutrophil x?df ;fwf/0ftof@ b]lv % j6f lobe x? /x]sf x¬G5g t/ % b]lv & j6f eGbf a9L lobe x? ePsf Neutrophil x?nfO{ Hyper segmented Neutrophil eg]/ elgG5 .
  • 8.
    s¬g} s¬g} Neutrophilx?df toxin sf] sf/0fn] Dark violet colour sf granule x? kfO{G5g tL Neutrophil x?nfO{ toxic granules ePsf Neutrophils (Neutrophils with toxic granules) elgG5 .
  • 9.
    olb peripheral bloodsmear df large Lymphocyte eGbf 7Ònf / dark blue cytoplasm ePsf Lymphocyte x? b]lvPdf ltlgx? nfO{ Atypical Lymphocyte elgG5 .
  • 10.
    #=Platelets Peripheral blood smeardf WBC / platelet sf] ;“Vof 1:10 b]lv 30 ;Ddsf] ratio df /x]sf] x¬G5 .
  • 11.
    Platelets eg]sf cellsf ;–;fgf 6¬qmfx? (fragments) x¬g h;sf] size 1-4 micron sf] x¬G5 t/ peripheral smear df Tof] eGbf 7Ònf] platelet x? b]lvPdf tL platelet x?nfO{ ghost cell eg]/ elgG5 .
  • 12.
    Smear df plateletsclump df jf separate u/]/ b¬a} form df kfpg ;lsG5 . 100x objective lens n] x]bf{ Pp6f microscopic field df slDtdf 15 b]lv 20 j6f platelets b]lvPdf platelets normal dflgG5 .