AMPK aids in fatty acid oxidation and helps prevent insulin resistance. Activated by exercise, AMPK facilitates stronger heart muscles and may prevent cardiac hypertrophy. The proposal hypothesizes that increased AMPK levels through prolonged exercise will increase mitochondrial density in type 1 muscle fibers and heart cells. Samples will be examined after rats exercise for 30 days to measure changes in AMPK levels and mitochondrial density compared to sedentary rats.

1. Functional role of enzyme AMPK
April Irums
GRANT PROPOSAL

2. AMP-activated
protein Kinase

4. • AMPK aids in efficient fatty
acid oxidation. (FAT LOSS!)

5. • protein synthesis • insulin resistance

6. Activated with exercise
Facilitates stronger heart muscles

7. Cardiac Hypertrophy
Diabetes
Faulty hormone signalling

8. What Do We Already Know?

9. Mitochondria

10. Hypothesis
Increased AMPK levels in
the body will increase
mitochondrial density in
type 1 muscle fibres and
myocytes

11. Examine Samples
AMPK Levels within Samples
Prolonged Exercise

12. NO exercise!Sedimentary Lifestyle

13. Use of Biopsy equipment
30 days to exercise Increase Heart Rate
Measure AMPK Levels
Rat Food

14. Muscle and Heart Biopsy

15. SUPER TINY SCAR!

16. Figuring out the functional role of
AMPK in the body!