Part 3 You run your experiment and look at the DNA replicated after two rounds. You separate plasmid DNA from the smaller dNTPs and measure plasmid radioactivity. To your disappointment, you get hot plasmid in the test tube with your drug. You show the results to your professor, who tells you that it's still possible that the antibiotic targets the DNA replication machinery. To figure out what may be happening, draw what you would expect to see in the first round of replication. Use the schematic on the left to work out what would happen during replication. Use thin lines for RNA and thick red lines for DNA. Please pay attention to the leading and lagging strands. Note that the 5 and 3 ' here refer to the template strand directionality. Next, fill out the schematic on the right, drawing what you expect to see after the first round of replication is completed. Use thick red lines for 'hot' DNA (and blue for 'cold' DNA). (Assume that if no new DNA is generated, the two strands of the original plasmid will re- anneal back together.) The yellow bars represent the origin of replication. 2. What would happen if you blocked the action of PRIMASE, so that it could no longer synthesize RNA? 3. What would happen if you blocked the action of DNA POLYMERASE III, so that it could not accomplish 5 3 polymerization? Pierce, Geneticr A Cancepruol Approoch, 7e 02020 W. H. Fieeman and Comparty. 4. What would happen if you blocked the action of DNA POLYMERASE 1, so that it could not accomplish 5 3 exonuclease reactions? Pierce, Genetics A Conceptuai Aeprooch, 7e 02020 W. H. Fretman and Compery 5. What would happen if you blocked the action of DNA LIGASE? Pierce, Genetice: A Concrptwod Apgroash, 7e 02020 W H. Freerwan and Conpacy.