Daniel K. Lesniewski has over 15 years of experience in organic synthesis, analytical chemistry, and process development. He holds an M.Sc. in Organic Chemistry from Colorado State University-Pueblo and a B.Sc. in Chemistry from the same institution. His experience includes positions at Corden Pharma, Avista Pharma Solutions, General Air, Amgen, Lexmark International, and Colorado State University, where he has experience in multi-step organic synthesis, analytical instrumentation, scale-up reactions, method development, and research.
The influence of data curation on QSAR Modeling – Presented at American Chemi...Kamel Mansouri
This presentation examined the impact of data quality on the construction of QSAR models being developed within the EPA‘s National Center for Computational Toxicology. We have developed a public-facing platform to provide access to predictive models. As part of the work we have attempted to disentangle the influence of the quality versus quantity of data available to develop and validate QSAR models. This abstract does not reflect U.S. EPA policy.
The State of Analytical Instruments in Some Environmental Pollution Control L...IJERA Editor
The state of the environmental laboratories involved in monitoring environmental pollution control in Nigeria has been studied in this research. The research was undertaken by visiting four analytical laboratories involved in environmental pollution control in Nigeria. The analytical laboratories visited are those of Nigerian National Petroleum Corporation (NNPC) Kaduna, Ashaka cement factory, regional laboratory of the Federal Ministry of Water Resources Gombe, and the National Reference laboratory Lagos. In these laboratories results were collected in the laboratories, interviews were carried out and analytical instruments available were documented. It was discovered that, in these laboratories many standard analytical instruments needed for quality environmental pollution control and monitoring are lacking. Comparison of analytical instruments found in these laboratories with those found in literature revealed that many needed analytical instruments are missing. It is the position of this work that the gap between the environmental analytical instruments found in literature and that found in the research laboratories is very large and calls for concern
The influence of data curation on QSAR Modeling – Presented at American Chemi...Kamel Mansouri
This presentation examined the impact of data quality on the construction of QSAR models being developed within the EPA‘s National Center for Computational Toxicology. We have developed a public-facing platform to provide access to predictive models. As part of the work we have attempted to disentangle the influence of the quality versus quantity of data available to develop and validate QSAR models. This abstract does not reflect U.S. EPA policy.
The State of Analytical Instruments in Some Environmental Pollution Control L...IJERA Editor
The state of the environmental laboratories involved in monitoring environmental pollution control in Nigeria has been studied in this research. The research was undertaken by visiting four analytical laboratories involved in environmental pollution control in Nigeria. The analytical laboratories visited are those of Nigerian National Petroleum Corporation (NNPC) Kaduna, Ashaka cement factory, regional laboratory of the Federal Ministry of Water Resources Gombe, and the National Reference laboratory Lagos. In these laboratories results were collected in the laboratories, interviews were carried out and analytical instruments available were documented. It was discovered that, in these laboratories many standard analytical instruments needed for quality environmental pollution control and monitoring are lacking. Comparison of analytical instruments found in these laboratories with those found in literature revealed that many needed analytical instruments are missing. It is the position of this work that the gap between the environmental analytical instruments found in literature and that found in the research laboratories is very large and calls for concern
The importance of data curation on QSAR Modeling: PHYSPROP open data as a cas...Kamel Mansouri
This presentation highlighted how data curation impacts the reliability of QSAR models. We examined key datasets related to environmental endpoints to validate across chemical structure representations (e.g., mol file and SMILES) and identifiers (chemical names and registry numbers), and approaches to standardize data into QSAR-ready formats prior to modeling procedures. This allowed us to quantify and segregate data into quality categories. This improved our ability to evaluate the resulting models that can be developed from these data slices, and to quantify to what extent efforts developing high-quality datasets have the expected pay-off in terms of predicting performance. The most accurate models that we build will be accessible via our public-facing platform and will be used for screening and prioritizing chemicals for further testing.
This presentation was a plenary talk on environmental forensics delivered at the 2011 Dioxin Conference in Brussels (www.dioxin2011.org). The presentation focused on the topic of environmental forensics investigations and techniques and their application to the field of persistent organic pollutants (POPs).
OECD Webinar | Assessing the dispersion stability and dissolution (rate) of n...OECD Environment
On Thursday 25 February 2021, Anne Gourmelon (Environment Directorate, OECD), Kathrin Schwirn (German Environment Agency, Umweltbundesamt, UBA); Frank von der Kammer (University of Vienna) Research and Development Center) and Doris Völker (German Environment Agency, Umweltbundesamt, UBA) presented the scope, content, and use of the Test Guideline No. 318: Dispersion Stability of Nanomaterials in Simulated Environmental Media and its accompanying Guidance Document. Further discussions focused on the scope of the upcoming Test Guideline.
The increased production and wide usage of manufactured nanomaterials suggest a higher probability of finding them in the environment. Therefore, testing the dissolution rate and dispersion stability for toxicity assessment are of paramount importance for adequate hazard assessment.
Sustainable chemistry is the design and use of chemicals that minimize impacts to human health, ecosystems and the environment. To assess sustainability, chemicals must be evaluated not only for their toxicity to humans and other species, but also for environmental persistence and potential formation of toxic products as a result of biotic and abiotic transformations. Traditional approaches to evaluate these characteristics are resource intensive and normally lack biologically mechanistic information that might facilitate a “safety by design” approach. A more promising approach would exploit recent advances in high-throughput (HT) and high-content (HC) screening methods coupled with computational methods for data analysis and predictive modelling. The elements of a framework to assess sustainable chemistry could rely on integration of non-testing approaches such as (Q)SAR and read-across, coupled with prediction models derived from HT/HC methods anchored to biological pathways (eg., Adverse Outcome Pathways). Acceptance and use of such integrated approaches necessitates a level of validation that demonstrates scientific confidence for specific decision contexts. Here we illustrate a scientific confidence framework for Tox21 approaches underpinned by a mechanistic basis, and illustrate how this will drive the development of enhanced non-testing approaches. This framework also focuses development of prediction models that are hybrid yet local in terms of their chemistry in nature. Specific examples highlight how the extensive testing library within ToxCast was profiled with respect to its chemistry, resulting in new insights that direct strategic testing as well as formulate new predictive models specifically SARs. This abstract does not necessarily reflect U.S. EPA policy.
Univ of Iowa Research on Best Methods to Detect Radioactivity in Marcellus Sh...Marcellus Drilling News
The results of a new study by researchers at the University of Iowa that looks at the best methods for detecting radioactivity levels in Marcellus Shale flowback water (wastewater from fracking). The researchers maintain federal EPA methods for testing drinking water are unreliable when used with frack wastewater and recommend a different, more reliable method.
Plenary talk at ISPAC conference on the use of polycyclic aromatic hydrocarbons (PAHs) in environmental forensics. Covers basics of what enviromental forensics investigations (EFIs) are and how PAHs can be used to help determine sources of releases (creosote, railway ties), oil sands development and oil spill releases (Macondo oil spill, gulf oil spill).
Organochlorine Pesticides in Fruits & Vegetables v2zq
Organochlorine Pesticides in Fruits & Vegetables - Resources for Healthy Children www.scribd.com/doc/254613619 - For more information, Please see Organic Edible Schoolyards & Gardening with Children www.scribd.com/doc/254613963 - Gardening with Volcanic Rock Dust www.scribd.com/doc/254613846 - Double Food Production from your School Garden with Organic Tech www.scribd.com/doc/254613765 - Free School Gardening Art Posters www.scribd.com/doc/254613694 - Increase Food Production with Companion Planting in your School Garden www.scribd.com/doc/254609890 - Healthy Foods Dramatically Improves Student Academic Success www.scribd.com/doc/254613619 - City Chickens for your Organic School Garden www.scribd.com/doc/254613553 - Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica www.scribd.com/doc/254613494 - Simple Square Foot Gardening for Schools - Teacher Guide www.scribd.com/doc/254613410 - Free Organic Gardening Publications www.scribd.com/doc/254609890 ~ pesticideresearch.com
Bhatia Brothers FZE is an Enrolled Electrical & Solar PV Consultant & Contractor for Shams Dubai, DEWA’s first smart initiative to connect solar energy to homes & buildings, a part of the Distributed Renewable Resources Generation program.
The importance of data curation on QSAR Modeling: PHYSPROP open data as a cas...Kamel Mansouri
This presentation highlighted how data curation impacts the reliability of QSAR models. We examined key datasets related to environmental endpoints to validate across chemical structure representations (e.g., mol file and SMILES) and identifiers (chemical names and registry numbers), and approaches to standardize data into QSAR-ready formats prior to modeling procedures. This allowed us to quantify and segregate data into quality categories. This improved our ability to evaluate the resulting models that can be developed from these data slices, and to quantify to what extent efforts developing high-quality datasets have the expected pay-off in terms of predicting performance. The most accurate models that we build will be accessible via our public-facing platform and will be used for screening and prioritizing chemicals for further testing.
This presentation was a plenary talk on environmental forensics delivered at the 2011 Dioxin Conference in Brussels (www.dioxin2011.org). The presentation focused on the topic of environmental forensics investigations and techniques and their application to the field of persistent organic pollutants (POPs).
OECD Webinar | Assessing the dispersion stability and dissolution (rate) of n...OECD Environment
On Thursday 25 February 2021, Anne Gourmelon (Environment Directorate, OECD), Kathrin Schwirn (German Environment Agency, Umweltbundesamt, UBA); Frank von der Kammer (University of Vienna) Research and Development Center) and Doris Völker (German Environment Agency, Umweltbundesamt, UBA) presented the scope, content, and use of the Test Guideline No. 318: Dispersion Stability of Nanomaterials in Simulated Environmental Media and its accompanying Guidance Document. Further discussions focused on the scope of the upcoming Test Guideline.
The increased production and wide usage of manufactured nanomaterials suggest a higher probability of finding them in the environment. Therefore, testing the dissolution rate and dispersion stability for toxicity assessment are of paramount importance for adequate hazard assessment.
Sustainable chemistry is the design and use of chemicals that minimize impacts to human health, ecosystems and the environment. To assess sustainability, chemicals must be evaluated not only for their toxicity to humans and other species, but also for environmental persistence and potential formation of toxic products as a result of biotic and abiotic transformations. Traditional approaches to evaluate these characteristics are resource intensive and normally lack biologically mechanistic information that might facilitate a “safety by design” approach. A more promising approach would exploit recent advances in high-throughput (HT) and high-content (HC) screening methods coupled with computational methods for data analysis and predictive modelling. The elements of a framework to assess sustainable chemistry could rely on integration of non-testing approaches such as (Q)SAR and read-across, coupled with prediction models derived from HT/HC methods anchored to biological pathways (eg., Adverse Outcome Pathways). Acceptance and use of such integrated approaches necessitates a level of validation that demonstrates scientific confidence for specific decision contexts. Here we illustrate a scientific confidence framework for Tox21 approaches underpinned by a mechanistic basis, and illustrate how this will drive the development of enhanced non-testing approaches. This framework also focuses development of prediction models that are hybrid yet local in terms of their chemistry in nature. Specific examples highlight how the extensive testing library within ToxCast was profiled with respect to its chemistry, resulting in new insights that direct strategic testing as well as formulate new predictive models specifically SARs. This abstract does not necessarily reflect U.S. EPA policy.
Univ of Iowa Research on Best Methods to Detect Radioactivity in Marcellus Sh...Marcellus Drilling News
The results of a new study by researchers at the University of Iowa that looks at the best methods for detecting radioactivity levels in Marcellus Shale flowback water (wastewater from fracking). The researchers maintain federal EPA methods for testing drinking water are unreliable when used with frack wastewater and recommend a different, more reliable method.
Plenary talk at ISPAC conference on the use of polycyclic aromatic hydrocarbons (PAHs) in environmental forensics. Covers basics of what enviromental forensics investigations (EFIs) are and how PAHs can be used to help determine sources of releases (creosote, railway ties), oil sands development and oil spill releases (Macondo oil spill, gulf oil spill).
Organochlorine Pesticides in Fruits & Vegetables v2zq
Organochlorine Pesticides in Fruits & Vegetables - Resources for Healthy Children www.scribd.com/doc/254613619 - For more information, Please see Organic Edible Schoolyards & Gardening with Children www.scribd.com/doc/254613963 - Gardening with Volcanic Rock Dust www.scribd.com/doc/254613846 - Double Food Production from your School Garden with Organic Tech www.scribd.com/doc/254613765 - Free School Gardening Art Posters www.scribd.com/doc/254613694 - Increase Food Production with Companion Planting in your School Garden www.scribd.com/doc/254609890 - Healthy Foods Dramatically Improves Student Academic Success www.scribd.com/doc/254613619 - City Chickens for your Organic School Garden www.scribd.com/doc/254613553 - Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica www.scribd.com/doc/254613494 - Simple Square Foot Gardening for Schools - Teacher Guide www.scribd.com/doc/254613410 - Free Organic Gardening Publications www.scribd.com/doc/254609890 ~ pesticideresearch.com
Bhatia Brothers FZE is an Enrolled Electrical & Solar PV Consultant & Contractor for Shams Dubai, DEWA’s first smart initiative to connect solar energy to homes & buildings, a part of the Distributed Renewable Resources Generation program.
The Nanaimo Yacht Charters & Sailing School management team has more than 30 years’ experience in delivering memorable charter holidays on a fleet of highly maintained sail and power yachts. It is the only Charter Company that has a full service division and boatyard.
If you have just had your first outing on a sailing yacht and are bemused by some of the names that all the different ropes are called? Well here is a quick explanation as to why rope is not just called rope!
Versatile and experienced chemist with seven years of expertise in research and development. Proficient in gas chromatography, mass spectrometry, HPLC, UVFNIL, ICPFAES, and wastewater analysis. Collaborative leader with demonstrated history of providing solutions to challenging problems facing industry managers and organizational teams. Bilingual in French and English.
OECD Global Forum on the Environment dedicated to Per- and Polyfluoroalkyl Su...OECD Environment
PFAS, which stands for per- and polyfluoroalkyl substances, are a diverse group of chemicals that include PFCAs, PFOA, PFSAs, PFHxS, and thousands of others. These chemicals have been in commercial production since the 1950s and are now widely used in consumer and industrial applications. One characteristic of PFAS is their persistence in the environment, as they are extremely resistant to degradation. PFAS have emerged as contaminants of global concern because of their potential to accumulate in the human body and food chains.
On 12-13 February 2024, a wide range of stakeholders, including governments, industry, non-governmental organisations (NGOs), and academics came together to discuss various topics related to PFAS. These topics covered areas such as country risk management approaches, innovation challenges for finding safer alternatives, effective risk communication strategies, monitoring techniques, waste management, and approaches to managing contamination.
Resume as of April 2015: Daniel Truong
If you represent a company and you see that I am a good fit, please don't hesitate to send me a message or email. Thank you.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
1. Daniel K. Lesniewski
201 Wright ST. Unit 205 Cell: 720-841-0826
Lakewood, CO 80228 email: dk.lesniewski@yahoo.com
EDUCATION: M.Sc. Organic Chemistry (emphasis: Fluoro-Organic Chemistry)
December 2009 Colorado State University – Pueblo, Colorado
B.Sc. Chemistry-Physical Organic Emphasis May 2006 (ACS certified)
Colorado State University-Pueblo, Colorado
SCIENTIFIC
INTRUMENTATION: GC-MS (5890 HP, Agilent), NMR (Varian INOVA 300 MHz, 500 MHz) and 60MHz Anasazi,
IR (Nicolet FTIR), UV-Vis, LCMS, HPLC, GC-FID (HP6890 GC), ICP-OES, Fluorescence,
GC-TCD (GOW-MAC 580 & 400), GC-DID (GOW-MAC 580), Rotary Evaporation, USP
Analysis, NF Analysis
EXPERIENCE:
01/04/2016 to current Process Development Chemist-Organic Synthesis (small molecule)
Corden Pharma, Boulder CO
• cGLP and cGMP development and manufacturing environment
• Designed and executed multi-step Organic synthesis reactions
• Performed pre-scale up front run and screen reactions
• Scale-up synthesis between 2-20 kg
• Weekly technical team meeting with customer to discuss progress and results
09/2015 to 12/21/2015 Process Development Chemist-Organic Synthesis (small molecule)
Avista Pharma Solutions, Longmont CO
• Worked in a cGLP and cGMP research laboratory environment
• Designed and executed multi-step Organic Synthesis reactions
• Monitored reactions, and molecular structure determination using HPLC, LCMS and 1H NMR
• Proof of concept and total multi-step synthesis of reference standard for phase-2 clinical program,
for cancer drug
• Scale-up synthesis between 50-100 g to make 40g of final compound in a 4-step synthesis
• Performed flash-column chromatography to isolate pure product
• Wrote weekly research reports and discussed results with the Director of Process Chemistry
• Regular collaboration with senior Process Chemists
• Weekly training seminars to discuss chemistry and work through mechanisms with the Director
of Process Chemistry
11/2014 to 09/2015 Research and Development Chemist
General Air, Commerce City CO
• Analytical method development for GC analysis of atmospheric and cryogenic gasses
• Followed FDA protocols and SOPs
• Thermodynamic calculations
• NF and USP analysis of medical, research and instrumental grade gasses (N, O, Ar, Ne, blood
gasses, lung diffusions, and specialty mixes.)
• Used GC-TCD, GC-DID and GC-PED to perform gas analysis (EZ-chrome software)
• Followed NF and USP analysis protocols
• General lab maintenance and instrument care
Page 1 of 6
2. • Regular collaboration with industry consultants and vendors
12/2013 to 6/2014 NREL BioEnergy Sciences Research Associate-Organic Retro-Synthesis
BioEnergy Sciences Center, National Renewable Energy Laboratory, Golden CO
• Worked on the development of Cellulose and Hemi-cellulose extraction from Bio-
material through chemical reactions and mechanical processes run in bioreactors.
• Assisted experienced scientists with deacetylation reactions and enzymatic hydrolysis
to release the component sugars from the biomass backbone for fermentation.
• Conducted acid impregnation reactions that helped breakup the lignin
• Operated large and small high pressure (50-300 psig) Bio- reactors.
• Operated lab equipment such as mills, fermentors, centrifuges, evaporators, pumps, and
filtration devices.
• Installed mechanical equipment and systems and calibrated sensors (temperature,
pressure, flow meters, pH meters.).
• Prepared chemical solutions and chemical reactor and bioreactor vessels for
experiments.
• Performed preventative equipment maintenance.
• Collected, prepared, and performed routine analyses of solid and liquid chemical
samples (moisture content, glucose concentration).
• Input experimental data into spreadsheets, and presented results to supervisor
• Kept lab area clean and maintained safe working conditions.
05/2013 to 11/2013 Manufacturing Associate II- Biotechnology/Drug Production
Amgen, Boulder Co
• Skilled operations in manufacturing plant. Manufacturing areas include Cell Culture,
Harvest, Media, Purification, Buffer Prep, and Dispensing. Hands on operations
including set-up, clean in-place (CIP), pressure test (PT), steam in-place (SIP), and
operation of large scale production equipment.
• Also monitored critical processes, execute validation protocols, and equipment
troubleshooting.
• Drafted and revised documents such as Manufacturing Procedures and SOPs. Assisted
in the review of documentation for assigned functions (e.g. equipment logs, routine area
audits, batch records) and completed tasks while complying with SOPs, cGMPs and
Amgen regulations.
09/2012 to 11/2012 Chemical Process Operator-Anodizing Department
Lexmark International, Contract through Bolder Staffing, Broomfield Co 80021
• Development and manufacturing of photoconductors for Lexmark electrophotography
technology.
• This position involved a variety of technical tasks in an automated manufacturing
environment where aluminum cylinders are coated with light-sensitive materials.
• Initiated and monitored automated processes
• Responded to system messages
• Set-up, tore-down, cleaned up, and installed complex process hardware
• Formulated solutions and mixtures of chemicals
• Measured the properties of liquids, dispersions, and coated cylinders using basic
laboratory equipment - Reviewed and classified defects in coated drums
• Interpreted test data and adjusted process conditions as needed
• Ensured compliance with procedures covering safety and environmental regulations
• Worked alone and with others to develop and modify operating procedures
Page 2 of 6
3. 01/2011 to 04/2011 Research and Development Polymer Chemist
Septodont, Louisville CO; Supervisor Dr. Marianela Trujillo
• Responsible for the design, synthesis, purification and characterization of molecules &
intermediates in methacrylate based polymers.
• Utilized analytical and chromatographic techniques for instance FT-IR, TLC, GC-
MS, flash chromatography, and NMR (500 MHz).
• Developed synthetic routes to help route realization and selection of processes for scale
up. Evaluated new synthesized materials.
05/2002 to 12/2009 Research Assistant-FluoroOrganic Chemistry
Colorado State University; Pueblo, Dept. of Chemistry, Research Advisor: Dr. Melvin Druelinger
Responsibilities:
• Responsible for a project studying the fluorination of alkenyl alcohols and five-membered
heteroaromatic compounds via electrophilic fluorination.
• Worked in a group setting on the discovery process of a synthetic methodology to
synthesize compounds which are potentially biologically active for medicinal purposes.
• Responsible for conducting all chemical synthesis, monitoring the reaction process via
N.M.R and GC-MS studies, analysis of results, and isolation of final products via column
chromatography.
• Responsible for interpreting, and communicating research results to the research group on a
routine basis.
Achievements:
• Contributed to the development of a synthetic method that produces potential bio-active
molecules via electrophilic fluorination. This new reaction process could lead to a new
class of pharmaceutical ingredients.
• Research accepted by the American Chemical Society for presentation of, “Monofluorination
of Alkenyl Alcohols using Electrophilic Fluorine as a synthetic route to Fluoroethers”,
Division of Chemical Education (Organic), Atlanta GA, March-26, 2006, and “Cyclic
Fluoroethers via the Electrophilic Fluorination of Alkenyl Alcohols”, at the ACS national
meeting, Division of Organic Chemistry (New reactions & Methodology, Heterocycles &
Aeromatics”), Anaheim CA, March 28, 2004.
• Contributed as lead student investigator to the development of a new synthetic method for
selectively placing fluorine in a molecule which may increase biological activity
• Publication of research in ACS journal pending.
01/1999 to 12/1999 Undergraduate Research Assistant-Atmospheric Studies
University of Colorado; Boulder, CO, Dept. of Chemistry, Research Advisor: Dr. Veronica Vaida
• Performed Atmospheric Processing Research and instructed peers on the proper techniques
of using and applying data modeling software for conducting research. These experiments
examined the effects of aerosols on the earth’s upper atmosphere
• Awarded the Camile and Henry Dreyfus Environmental Fellowship. This fellowship
involved chemical research at the interface between chemistry and environmental science
The specific project entailed spectroscopic studies of the organic radicals at interfaces to
provide the fundamental data base needed to model atmospheric organic aerosols
Page 3 of 6
4. ACS PRESENTATIONS: - 231st
ACS National Meeting, Division of Chemical Education, March 26-30, 2006
Atlanta, GA
- 227th
ACS National Meeting, Division of Organic Chemistry, March 28-April 1, 2004
Anaheim, CA
PUBLICATIONS: W. Brown, B. Wallin, DK. Lesniewski, D. Gooding and J. Martin; “The Experimental
Determination of On-Off Keying Laser Communications Probability Models and a Comparison
with Theory”, submitted for pear review on November 30, 2005 in the Journal of Applied
Optics.
HONORS: - Sigma Xi Research Honor Society Associate level member (2008)
- Dean’s List (2003)
- Camile and Henry Dreyfus Scientific Research Fellowship (1999)
COMPUTERS: ChemDraw, PC-Spartan, Sci-Finder Scholar, Word, Excel, Access, Power Point, IDL, Hitran, Acrobat,
Outlook, CorelDraw
LANGUAGES: Polish fluent
OTHER: Chemistry Tutor, ACS Member
Page 4 of 6
5. REFERENCES:
Weidong Liu, Ph.D
Director of Process Chemistry
AVISTA Pharma Solutions
T: 303-386-1271
M: 303-667-7086
weidong.liu@avistapharma.com
Todd Eary, Ph.D.
Executive Director Process Chemistry and Pharmaceutics
AVISTA Pharma Solutions
T: 303-386-1101
M: 720-935-2983
teary@avistapharma.com
Marianela Trujillo, Ph.D.
Research and Development Polymer Chemist
Research and Development Manager
Septodont, Louisville CO
303-516-9075 ext 26
marianelatrujillo@yahoo.com
marianelatrujillolemon@gmail.com
Melvin Druelinger, Ph.D.
Dean of Graduate Studies and Research
Director of Research and Sponsored Programs
Professor of Chemistry
Research Advisor
Colorado State University-Pueblo
2200 Bonforte Blvd
Pueblo, CO 81001-4901
Office: 719-549-2166
e-mail: mel.druelinger@colostate-pueblo.edu
David Dillon, Ph.D.
Professor of Chemistry
Colorado State University-Pueblo
Page 5 of 6
6. 2200 Bonforte Blvd
Pueblo, CO 81001-4901
Office: 719-549-2678
e-mail: david.dillon@colostate-pueblo.edu
Bill Brown, Ph.D
Professor of Physics
Colorado State University-Pueblo
2200 Bomforte Blvd
Pueblo, CO 81001
Office: 719-549-2683
e-mail: William.brown@colostate-pueblo.edu
Page 6 of 6
7. 2200 Bonforte Blvd
Pueblo, CO 81001-4901
Office: 719-549-2678
e-mail: david.dillon@colostate-pueblo.edu
Bill Brown, Ph.D
Professor of Physics
Colorado State University-Pueblo
2200 Bomforte Blvd
Pueblo, CO 81001
Office: 719-549-2683
e-mail: William.brown@colostate-pueblo.edu
Page 6 of 6
