This document summarizes a study that isolated cellulose from salacca midrib fibers through chemical treatments. The fibers were treated with sodium hydroxide solution followed by hydrogen peroxide bleaching. This increased the cellulose content from 47.18% to 51.81% while decreasing lignin. Characterization through FTIR, SEM and XRD showed reduced impurities and increased crystallinity index from 62.4% to 70.04% after treatments. The isolation process demonstrated an effective method of extracting pure cellulose from salacca midrib fibers through alkali and bleaching treatments.