This study investigated the relationship between MMP-9 gene expression and sialic acid (NANA) in human glial cell lines. Researchers treated glial cell lines with varying concentrations of NANA and found that MMP-9 mRNA expression increased with treatment, indicating NANA may be involved in signaling pathways regulating MMP-9 expression. The results suggest determining how NANA influences MMP-9 and other inflammatory molecules could provide insight into neurodegenerative diseases involving inflammation.

1. *Corresponding Author: Hamid Galedari 118
1st
International Conference on Medicine, Public Health and Biological Sciences (MPHBS)
Study of MMP-9 gene expression in the presence of Sialic acid in human Glial
cell line
Narjes Khatoun Shabani Sadr1
, Mouhammad Shafiei1
, Hamid Galedari1,*
, Alireza Khirolah2
1
Department of Genetic, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
2
Department of Biochemistry, Medical School, Cellular and Molecular Research Center, Ahvaz Jundishapur
University of Medical Science, Ahvaz, Iran.
Abstract
Introduction: Sialic acid (N-acetylneuraminic acid, NANA) is found at all cell surfaces of vertebrates which can
cause many different reactions, such as starting or inhibiting the immune response, the activating of the
complement system and cell signaling. Sialic acid involvement in the signaling pathways leading to MMP-9
expression in Glial cells is unclear. Therefore, this study investigated the relationship between MMP-9 and NANA
and the effects of these ligand on the signaling processes of the inflammatory demyelination. Materials and
methods: Human Glial cell line was prepared from “Pasteur Institute of Iran” and cultured in DMEM,
supplemented with 10% FBS. The IC50 value of NANA was obtained by MTT assay. Glial cell line was treated with
NANA (300,500 and 1000 µg/ml) for 24h to investigate the effects of these ligand on the expression of MMP-9.
Then total cellular RNA was isolated from approximately 6-5×106 Glial cells. 1000 ng of total RNA from each
sample was used into cDNA. Real-time PCR determined the expression level of the MMP-9 transcripts and REST
and SPSS software were used for analyze. Results: The results of MTT assay were analyzed by Excel software and
IC50 was obtained equivalent to 1273.3 µM. Therefore, the concentration of Sialic acid was selected for
treatments that were less than Ic50 (300,500 and 1000 µM). By analyzing Real-time data, it was found that MMP-9
mRNA expression was up-regulated with treatment and indicated a possible involvement NANA on signaling
pathway this gene expression. Conclusion: By determining the possible relationship between NANA and MMP-9
gene expression, which is one of a key inflammation factors in some neurodegenerative diseases, should be
investigated MMPs gene control agents (including TIMPs and miRNAs); as well as other inflammatory
macromolecules such as cytokines, chemokines, prostaglandins, NO and ROS.
© 2016 Published by CASRP publishing company Ltd. UK. Selection and/or peer-review under responsibility of
Center of Advanced Scientific Research and Publications Ltd. UK.
Keywords: Sialic acid (N-acetylneuraminic acid), NANA, MMP-9, Glial cell line, Multiple Sclerosis (MS).
How to cite this article: Shabani Sadr, N.K., Shafiei, M., Galedari, H., Khirolah, A., 2016. Study of MMP-9 gene
expression in the presence of Sialic acid in human Glial cell line. 1st International Conference on Medicine, Public
Health and Biological Sciences (MPHBS), Sep. 2016.
Access this article online
Website: www.casrp.co.uk/conferences
DOI: 10.18869/MPHBS.2016.118
Available online at http://www.casrp.co.uk/conferences
CASRP Publisher