micropropogation
•Download as PPT, PDF•
0 likes•2 views
Tissue culture and micropropogation slide share # Welcome To My Profile .... My Bio Can't Explain Me Briefly !!!
Report
Share
Report
Share
Recommended
Micropropagasi
Micropropagation is the process of rapidly multiplying plant materials in vitro. It involves taking plant cells, tissues or organs as explants and culturing them on nutrient media to stimulate growth. Common micropropagation techniques include axillary shoot proliferation from pre-existing meristems using cytokinin treatment, shoot organogenesis from leaf or stem explants, and somatic embryogenesis from callus or suspension cells. Micropropagation has advantages like rapid multiplication, disease elimination, and production of propagules with desirable traits, but also limitations like high equipment and expertise costs. It has applications for quickly increasing stocks of new varieties and producing disease-free plants.
Microprop
The document discusses various micropropagation techniques for multiplying plants in vitro, including cloning plants from explants, axillary shoot proliferation using cytokinins, and dealing with contamination issues. It also compares the advantages and limitations of micropropagation versus conventional propagation methods, and lists some applications of micropropagation like rapid multiplication of superior varieties and producing disease-free plants.
Plant tissue culture in crop Improvement
Tissue culture is a technique used in crop improvement involving growing plant cells, tissues or organs in vitro under sterile conditions. It allows for rapid mass propagation of plants, production of disease-free planting material, and genetic improvement through techniques like protoplast fusion and somatic hybridization. Some key applications of tissue culture discussed are micropropagation, germplasm conservation, haploid and dihaploid production, embryo rescue, artificial seed production, and overcoming barriers to wide hybridization. While a powerful tool, tissue culture must be done carefully to avoid spreading pathogens and maintain genetic integrity of regenerated plants.
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...A Biodiction : A Unit of Dr. Divya Sharma
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disadvantages
Multiplication of Apical or Axillary bud, Shoot tip or meristem culture
Production of Disease free plants by Micropropagation techniques: their Advantages and DisadvantagesMicropropagation
Micropropagation is a tissue culture technique used for the rapid asexual propagation of plants. It involves culturing small pieces of plant tissues or organs on growth media in controlled conditions. The process includes initiation of cultures from explants, multiplication through shoot proliferation or somatic embryogenesis, rooting of shoots, and transplantation of plantlets to soil. Micropropagation allows for large-scale production of genetically identical clones in a short period of time. It has applications in producing disease-free plants, conserving rare species, and commercializing new plant varieties.
Plant tissue culture,its methods, advantages,disadvantages and applications.
Plant tissue culture is the most widely used technique for growing very large number of plant using a very small part of the main plant(explant). Tissue culturing is very common for many popular and demanding crops.Few of them discussed here are Potato,Papaya,Pinepple,Banana,Gerbera,Sunflower,Orchids
Micropropagation
This document discusses micropropagation, which is the rapid vegetative propagation of plants using modern tissue culture methods to produce genetically identical copies. It can be used to multiply genetically modified plants, overcome limitations of conventional breeding, and provide sufficient plantlets from stock plants that do not produce seeds or respond well to other propagation. The key methods are multiplication through meristematic tissue, adventitious shoots, somatic embryogenesis, and organogenesis. Micropropagation has commercial uses and significance in producing disease-free plants year-round, exchanging germplasm internationally, conserving genetics, and producing synthetic seeds. While expensive, it provides uniformity and allows maintaining germplasm stocks for years.
Cell and tissue culture
Plant tissue culture involves growing plant cells, tissues, or organs in sterile conditions on a nutrient medium. It allows for mass production of plant clones and regeneration of whole plants from modified plant cells. Key aspects of plant tissue culture include using explants, maintaining sterile conditions, and promoting cell differentiation and regeneration into whole plants using techniques like micropropagation and somatic embryogenesis. Success requires selecting the right tissue culture type and optimizing factors like media, light, and temperature for the specific plant.
Recommended
Micropropagasi
Micropropagation is the process of rapidly multiplying plant materials in vitro. It involves taking plant cells, tissues or organs as explants and culturing them on nutrient media to stimulate growth. Common micropropagation techniques include axillary shoot proliferation from pre-existing meristems using cytokinin treatment, shoot organogenesis from leaf or stem explants, and somatic embryogenesis from callus or suspension cells. Micropropagation has advantages like rapid multiplication, disease elimination, and production of propagules with desirable traits, but also limitations like high equipment and expertise costs. It has applications for quickly increasing stocks of new varieties and producing disease-free plants.
Microprop
The document discusses various micropropagation techniques for multiplying plants in vitro, including cloning plants from explants, axillary shoot proliferation using cytokinins, and dealing with contamination issues. It also compares the advantages and limitations of micropropagation versus conventional propagation methods, and lists some applications of micropropagation like rapid multiplication of superior varieties and producing disease-free plants.
Plant tissue culture in crop Improvement
Tissue culture is a technique used in crop improvement involving growing plant cells, tissues or organs in vitro under sterile conditions. It allows for rapid mass propagation of plants, production of disease-free planting material, and genetic improvement through techniques like protoplast fusion and somatic hybridization. Some key applications of tissue culture discussed are micropropagation, germplasm conservation, haploid and dihaploid production, embryo rescue, artificial seed production, and overcoming barriers to wide hybridization. While a powerful tool, tissue culture must be done carefully to avoid spreading pathogens and maintain genetic integrity of regenerated plants.
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disad...A Biodiction : A Unit of Dr. Divya Sharma
Clonal Propagation: Introduction, Techniques, Factors, Applications and Disadvantages
Multiplication of Apical or Axillary bud, Shoot tip or meristem culture
Production of Disease free plants by Micropropagation techniques: their Advantages and DisadvantagesMicropropagation
Micropropagation is a tissue culture technique used for the rapid asexual propagation of plants. It involves culturing small pieces of plant tissues or organs on growth media in controlled conditions. The process includes initiation of cultures from explants, multiplication through shoot proliferation or somatic embryogenesis, rooting of shoots, and transplantation of plantlets to soil. Micropropagation allows for large-scale production of genetically identical clones in a short period of time. It has applications in producing disease-free plants, conserving rare species, and commercializing new plant varieties.
Plant tissue culture,its methods, advantages,disadvantages and applications.
Plant tissue culture is the most widely used technique for growing very large number of plant using a very small part of the main plant(explant). Tissue culturing is very common for many popular and demanding crops.Few of them discussed here are Potato,Papaya,Pinepple,Banana,Gerbera,Sunflower,Orchids
Micropropagation
This document discusses micropropagation, which is the rapid vegetative propagation of plants using modern tissue culture methods to produce genetically identical copies. It can be used to multiply genetically modified plants, overcome limitations of conventional breeding, and provide sufficient plantlets from stock plants that do not produce seeds or respond well to other propagation. The key methods are multiplication through meristematic tissue, adventitious shoots, somatic embryogenesis, and organogenesis. Micropropagation has commercial uses and significance in producing disease-free plants year-round, exchanging germplasm internationally, conserving genetics, and producing synthetic seeds. While expensive, it provides uniformity and allows maintaining germplasm stocks for years.
Cell and tissue culture
Plant tissue culture involves growing plant cells, tissues, or organs in sterile conditions on a nutrient medium. It allows for mass production of plant clones and regeneration of whole plants from modified plant cells. Key aspects of plant tissue culture include using explants, maintaining sterile conditions, and promoting cell differentiation and regeneration into whole plants using techniques like micropropagation and somatic embryogenesis. Success requires selecting the right tissue culture type and optimizing factors like media, light, and temperature for the specific plant.
Clonal propagation in ornamental plants
1) Clonal propagation is the multiplication of genetically identical copies of a plant cultivar through asexual reproduction. This can be done in vivo through methods like cutting, layering, and grafting, or in vitro through tissue culture techniques.
2) Micropropagation through tissue culture involves taking explants like shoot tips or meristems and culturing them on nutrient media to induce multiplication. Common methods are meristem culture, organogenesis, and somatic embryogenesis.
3) Clonal propagation has advantages like producing true-to-type plants, allowing off-season production, reducing life cycles, enabling large-scale production, and facilitating germplasm conservation and genetic transformation. However, it can be costly and labor intensive
agribiotech20063.ppt
dkjcvdcvbwcknewkcjewjcndncc ecuhewc qeubcuiqhdiu echuiewuihuieqh heuihuiewhuidh whuidhewhew heuchuewuhcu dueihfuiewhv ewiuewufhuiewh wehuifhewuihfui ewuhufih wehufewhd iuryy ry7y r2y uy99 y94y924 94ry4928r 89ry8249ry2489r y289ry24r9248r r28r92r 982 dhfuidhfuidf dfhdfhusig dsufhuidfduisfuih suihsdui
Plant tissue culture (PTC)
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. The key hormones auxin and cytokinin play important roles in differentiation. Tissue culture applications include micropropagation, germplasm preservation, haploid production, and genetic engineering. Important techniques include somatic embryogenesis, organogenesis, microcutting, anther/microspore culture, protoplast culture, and callus and cell suspension culture.
Micro propagation
Micropropagation, also known as tissue culture, is a method of rapidly multiplying plant materials using aseptic laboratory techniques to produce many clonal progeny. Key aspects of micropropagation include taking explants from stock plants and culturing them on nutrient media, proliferating shoots in a multiplication stage, and rooting the shoots to produce clonal plantlets. This allows for mass production of genetically identical plant materials year-round while eliminating diseases.
Bio 130 tissue culture --intro lecture dev strategies part i tissue cultue 2...
The document discusses plant propagation through both sexual and asexual life cycles. The sexual cycle involves the fusion of gametes to produce seeds with unique genetics. The asexual cycle uses mitosis to produce clones with identical genetics to the parent. Common asexual propagation methods include stolons, rhizomes, bulbs, and tissue culture. Tissue culture techniques allow for mass propagation of plant varieties and pathogen removal. Key factors for successful tissue culture include explant selection, sterilization, and a nutrient medium with appropriate growth regulators like auxins and cytokinins. Fern spores provide a simple model for studying cell differentiation and gametophyte development in tissue culture.
Tissue culture
The document discusses various tissue culture techniques used in plant breeding including: clonal propagation of disease-free genetic stocks through tissue culture; freeze preservation of germplasm; embryo, ovule, and anther culture techniques to produce haploid plants; and the induction of genetic variability through cell cultures. It provides details on the basic procedures of plant tissue culture including establishment of aseptic culture from explants, proliferation of callus cells on nutrient media, rooting, and acclimatization of regenerated plantlets. The roles of growth hormones, nutrient media composition, and factors affecting culture efficiency are also summarized.
Hairy root culture, multiple shoot culture ,
Definition of hairy root culture ,multiple shoot culture ,Production of hairy root and multiple shoot , advantages an disadvantages of hairy root and multiple shoot culture, Sterilization and sterilizing agents wit concentration and exposure time
Micropropagation
Micropropagation (tissue culture or invitro culture) refers to the multiplication of plants, in an aseptic condition and in artificial growth medium from plant parts like meristem tip, callus, embryos anthers, axillary buds etc. It is a method by which a true to type and disease free entire plant can be regenerated from a miniature piece of plant in aseptic condition in artificial growing medium rapidly throughout the year.
Plant Tissue Culture Technique and its applications
Plant tissue culture and its application on horticultural crops.it is the best method to grow the crops in high number especially the highly demanding ones.
application of plant tissue culture
Tissue culture is a technique used to grow plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. It has various applications in horticulture including micropropagation, germplasm conservation, haploid and dihaploid production, embryo rescue and synthetic seed production. The process involves selecting an explant from a mother plant, inducing callus formation, initiating shoot and root development, and acclimatizing the plantlets. Factors like the growth medium, environmental conditions and explant source influence the outcome. Tissue culture has advantages like rapid mass propagation of disease-free clones and conservation of endangered species, but also risks of genetic variation and infection if not performed carefully.
Plant Tissue Culture Technique and its applications
Plant tissue culture technique and its commercial application on horticultural crops.It is the best technique used all over the world to produce the replica of the plant in high number especially used for highly demanding crops.
Introduction of plant tissue culture
Plant tissue culture provides several benefits for studying plant growth and development. It allows scientists to isolate plant parts and culture them in vitro, simplifying the study of controlling influences. Some key applications of plant tissue culture include clonal propagation of disease-free plants, studying plant cells' ability to regenerate whole plants from cultured cells, producing genetic variability through somaclonal variation, regenerating plants from pollen to create haploids, and rescuing hybrid embryos. Plant tissue culture also enables fundamental biological research, production of high-value biochemicals, and generation of transgenic plants.
Application of tissue culture in crop improvement
Tissue culture techniques can be used for crop improvement in several ways:
1) They allow for wide hybridization through techniques like in vitro fertilization, embryo culture, and protoplast fusion to overcome genetic barriers.
2) They enable the production of haploids which are useful for developing homozygous lines.
3) Somaclonal variation generated in tissue culture can provide traits with agricultural importance.
4) Micropropagation allows for large-scale propagation of disease-free plants.
4638063.ppt
Plant tissue culture techniques allow for the growth and development of plant cells, tissues, organs, or protoplasts in sterile conditions on nutrient media. There are several types of in vitro culture including callus culture, organ culture, and somatic embryogenesis. Plant cells have the properties of totipotency, dedifferentiation, and competency which allow regeneration of whole plants from single cells. Tissue culture is important for applications such as crop improvement, mass propagation, and germplasm conservation.
Plant tissue culture (1)
Tissue culture is the process of growing plant cells, tissues or organs in an artificial, sterile environment. It involves removing plant cells and placing them in a nutrient solution supplemented with hormones, vitamins and minerals. Key requirements include using appropriate explant tissue, a suitable growth medium, aseptic conditions, and growth regulators. The document outlines various tissue culture techniques such as micropropagation, callus culture, somatic embryogenesis, organogenesis, and anther/pollen/ovule/ovary culture. Factors affecting successful tissue culture include the plant genotype, explant source, nutrient composition of the growth medium, and control of environmental factors like light, temperature and sterility.
Tissue culture applications
The document discusses various applications of tissue culture techniques including producing virus-free plants through heat treatment, meristemming, and using single cells to regenerate shoots; micropropagation to rapidly produce clones; somaclonal variation to induce genetic mutations; and synthetic seeds to efficiently transport and germinate plant materials. Micropropagation is described as being faster but more expensive than conventional propagation methods, and somaclonal variation can generate heritable or non-heritable variations for crop improvement.
Different types of Explants in Tissue Culture.pptx
Much faster rates of growth can be induced in vitro than by traditional means.
Multiplication of plants which are very difficult to propagate by cuttings or other traditional methods.
Production of large numbers of genetically identical clones in a short time
Seeds can be germinated with no risk of damping off/ predation.
Under certain conditions, plant material can be stored in vitro for considerable periods of time with little or no maintenance
Tissue culture techniques are used for virus eradication, genetic manipulation, somatic hybridization and other procedures that benefit propagation, crop improvement, and basic research.
By means of tissue culture it is possible to produce pathogen free plantlets by mass multiplication in a very limited amount of area from a very small sterile part of a mother plant. This method is also used to produce/ multiply plants that are to be transported across national border and so for their faster multiplication.But the establishment of a tissue culturing unit needs huge financial investments, skilled labors/technicians and required areas for its establishment are major constraints. Plant tissues grow and multiply in the labs only when there is an uncompetitive, growing condition with uninterrupted supply of nutrients.
Medium:
It contains all the elements that contribute the required nutrients that aid to the growth of the tissues; it is in liquid state or semi-solid in nature. The tissues are grown on the media. It consists of 95% of water, major and minor nutrients, plant growth hormones, vitamins, sugar rich compounds and chelating agents.
Totipotency:
It is the ability of a tissue or an organ of a plant to produce the whole plant, under the optional laboratory conditions and this is called as Totipotency. This is the baseline over which plant tissue culture relies upon.
Callus Culture:
When the cells divide into an undifferentiated mass it is called as callus. Any part of a plant can be used to produce the calli. It may be a stem, leaf, meristem or any other part. It is used to produce variations among the plantlets.
Suspension culture:
The callus produced from the explants are grown on nutrient solutions (that are semi solid) for a period of time and they are induced to produce plants with new traits.
Embryo Culture:
The method of culturing mature and immature embryos in media is called embryo culture. By this method, it is possible to produce plants from dormant seeds and seeds with metabolites that inhibit germination. This method is very important in crop improvement programs.
Somatic Embryogenesis:
When the plants are grown on nutrient media, calli are formed. When these calli are subjected to growth in cytokinin medium, somatic embryos are formed. They are circular, elongated,
Single cell culture
• As stated earlier, cells derived from a single cell through mitosis constitute a clone and the process of obtaining clones is called cloning (asexual progeny of a single individual make up.
Introduction to plant tissue culture...
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It allows for clonal propagation, production of secondary metabolites, induction of genetic variability, and regeneration of plants from somatic embryos or protoplasts. Key applications of plant tissue culture include micropropagation, production of pharmaceuticals, haploid production, somatic hybridization, transgenic plant production, germplasm conservation, breaking seed dormancy, and biomass energy production.
More Related Content
Similar to micropropogation
Clonal propagation in ornamental plants
1) Clonal propagation is the multiplication of genetically identical copies of a plant cultivar through asexual reproduction. This can be done in vivo through methods like cutting, layering, and grafting, or in vitro through tissue culture techniques.
2) Micropropagation through tissue culture involves taking explants like shoot tips or meristems and culturing them on nutrient media to induce multiplication. Common methods are meristem culture, organogenesis, and somatic embryogenesis.
3) Clonal propagation has advantages like producing true-to-type plants, allowing off-season production, reducing life cycles, enabling large-scale production, and facilitating germplasm conservation and genetic transformation. However, it can be costly and labor intensive
agribiotech20063.ppt
dkjcvdcvbwcknewkcjewjcndncc ecuhewc qeubcuiqhdiu echuiewuihuieqh heuihuiewhuidh whuidhewhew heuchuewuhcu dueihfuiewhv ewiuewufhuiewh wehuifhewuihfui ewuhufih wehufewhd iuryy ry7y r2y uy99 y94y924 94ry4928r 89ry8249ry2489r y289ry24r9248r r28r92r 982 dhfuidhfuidf dfhdfhusig dsufhuidfduisfuih suihsdui
Plant tissue culture (PTC)
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. The key hormones auxin and cytokinin play important roles in differentiation. Tissue culture applications include micropropagation, germplasm preservation, haploid production, and genetic engineering. Important techniques include somatic embryogenesis, organogenesis, microcutting, anther/microspore culture, protoplast culture, and callus and cell suspension culture.
Micro propagation
Micropropagation, also known as tissue culture, is a method of rapidly multiplying plant materials using aseptic laboratory techniques to produce many clonal progeny. Key aspects of micropropagation include taking explants from stock plants and culturing them on nutrient media, proliferating shoots in a multiplication stage, and rooting the shoots to produce clonal plantlets. This allows for mass production of genetically identical plant materials year-round while eliminating diseases.
Bio 130 tissue culture --intro lecture dev strategies part i tissue cultue 2...
The document discusses plant propagation through both sexual and asexual life cycles. The sexual cycle involves the fusion of gametes to produce seeds with unique genetics. The asexual cycle uses mitosis to produce clones with identical genetics to the parent. Common asexual propagation methods include stolons, rhizomes, bulbs, and tissue culture. Tissue culture techniques allow for mass propagation of plant varieties and pathogen removal. Key factors for successful tissue culture include explant selection, sterilization, and a nutrient medium with appropriate growth regulators like auxins and cytokinins. Fern spores provide a simple model for studying cell differentiation and gametophyte development in tissue culture.
Tissue culture
The document discusses various tissue culture techniques used in plant breeding including: clonal propagation of disease-free genetic stocks through tissue culture; freeze preservation of germplasm; embryo, ovule, and anther culture techniques to produce haploid plants; and the induction of genetic variability through cell cultures. It provides details on the basic procedures of plant tissue culture including establishment of aseptic culture from explants, proliferation of callus cells on nutrient media, rooting, and acclimatization of regenerated plantlets. The roles of growth hormones, nutrient media composition, and factors affecting culture efficiency are also summarized.
Hairy root culture, multiple shoot culture ,
Definition of hairy root culture ,multiple shoot culture ,Production of hairy root and multiple shoot , advantages an disadvantages of hairy root and multiple shoot culture, Sterilization and sterilizing agents wit concentration and exposure time
Micropropagation
Micropropagation (tissue culture or invitro culture) refers to the multiplication of plants, in an aseptic condition and in artificial growth medium from plant parts like meristem tip, callus, embryos anthers, axillary buds etc. It is a method by which a true to type and disease free entire plant can be regenerated from a miniature piece of plant in aseptic condition in artificial growing medium rapidly throughout the year.
Plant Tissue Culture Technique and its applications
Plant tissue culture and its application on horticultural crops.it is the best method to grow the crops in high number especially the highly demanding ones.
application of plant tissue culture
Tissue culture is a technique used to grow plant cells, tissues or organs in an artificial nutrient medium under sterile conditions. It has various applications in horticulture including micropropagation, germplasm conservation, haploid and dihaploid production, embryo rescue and synthetic seed production. The process involves selecting an explant from a mother plant, inducing callus formation, initiating shoot and root development, and acclimatizing the plantlets. Factors like the growth medium, environmental conditions and explant source influence the outcome. Tissue culture has advantages like rapid mass propagation of disease-free clones and conservation of endangered species, but also risks of genetic variation and infection if not performed carefully.
Plant Tissue Culture Technique and its applications
Plant tissue culture technique and its commercial application on horticultural crops.It is the best technique used all over the world to produce the replica of the plant in high number especially used for highly demanding crops.
Introduction of plant tissue culture
Plant tissue culture provides several benefits for studying plant growth and development. It allows scientists to isolate plant parts and culture them in vitro, simplifying the study of controlling influences. Some key applications of plant tissue culture include clonal propagation of disease-free plants, studying plant cells' ability to regenerate whole plants from cultured cells, producing genetic variability through somaclonal variation, regenerating plants from pollen to create haploids, and rescuing hybrid embryos. Plant tissue culture also enables fundamental biological research, production of high-value biochemicals, and generation of transgenic plants.
Application of tissue culture in crop improvement
Tissue culture techniques can be used for crop improvement in several ways:
1) They allow for wide hybridization through techniques like in vitro fertilization, embryo culture, and protoplast fusion to overcome genetic barriers.
2) They enable the production of haploids which are useful for developing homozygous lines.
3) Somaclonal variation generated in tissue culture can provide traits with agricultural importance.
4) Micropropagation allows for large-scale propagation of disease-free plants.
4638063.ppt
Plant tissue culture techniques allow for the growth and development of plant cells, tissues, organs, or protoplasts in sterile conditions on nutrient media. There are several types of in vitro culture including callus culture, organ culture, and somatic embryogenesis. Plant cells have the properties of totipotency, dedifferentiation, and competency which allow regeneration of whole plants from single cells. Tissue culture is important for applications such as crop improvement, mass propagation, and germplasm conservation.
Plant tissue culture (1)
Tissue culture is the process of growing plant cells, tissues or organs in an artificial, sterile environment. It involves removing plant cells and placing them in a nutrient solution supplemented with hormones, vitamins and minerals. Key requirements include using appropriate explant tissue, a suitable growth medium, aseptic conditions, and growth regulators. The document outlines various tissue culture techniques such as micropropagation, callus culture, somatic embryogenesis, organogenesis, and anther/pollen/ovule/ovary culture. Factors affecting successful tissue culture include the plant genotype, explant source, nutrient composition of the growth medium, and control of environmental factors like light, temperature and sterility.
Tissue culture applications
The document discusses various applications of tissue culture techniques including producing virus-free plants through heat treatment, meristemming, and using single cells to regenerate shoots; micropropagation to rapidly produce clones; somaclonal variation to induce genetic mutations; and synthetic seeds to efficiently transport and germinate plant materials. Micropropagation is described as being faster but more expensive than conventional propagation methods, and somaclonal variation can generate heritable or non-heritable variations for crop improvement.
Different types of Explants in Tissue Culture.pptx
Much faster rates of growth can be induced in vitro than by traditional means.
Multiplication of plants which are very difficult to propagate by cuttings or other traditional methods.
Production of large numbers of genetically identical clones in a short time
Seeds can be germinated with no risk of damping off/ predation.
Under certain conditions, plant material can be stored in vitro for considerable periods of time with little or no maintenance
Tissue culture techniques are used for virus eradication, genetic manipulation, somatic hybridization and other procedures that benefit propagation, crop improvement, and basic research.
By means of tissue culture it is possible to produce pathogen free plantlets by mass multiplication in a very limited amount of area from a very small sterile part of a mother plant. This method is also used to produce/ multiply plants that are to be transported across national border and so for their faster multiplication.But the establishment of a tissue culturing unit needs huge financial investments, skilled labors/technicians and required areas for its establishment are major constraints. Plant tissues grow and multiply in the labs only when there is an uncompetitive, growing condition with uninterrupted supply of nutrients.
Medium:
It contains all the elements that contribute the required nutrients that aid to the growth of the tissues; it is in liquid state or semi-solid in nature. The tissues are grown on the media. It consists of 95% of water, major and minor nutrients, plant growth hormones, vitamins, sugar rich compounds and chelating agents.
Totipotency:
It is the ability of a tissue or an organ of a plant to produce the whole plant, under the optional laboratory conditions and this is called as Totipotency. This is the baseline over which plant tissue culture relies upon.
Callus Culture:
When the cells divide into an undifferentiated mass it is called as callus. Any part of a plant can be used to produce the calli. It may be a stem, leaf, meristem or any other part. It is used to produce variations among the plantlets.
Suspension culture:
The callus produced from the explants are grown on nutrient solutions (that are semi solid) for a period of time and they are induced to produce plants with new traits.
Embryo Culture:
The method of culturing mature and immature embryos in media is called embryo culture. By this method, it is possible to produce plants from dormant seeds and seeds with metabolites that inhibit germination. This method is very important in crop improvement programs.
Somatic Embryogenesis:
When the plants are grown on nutrient media, calli are formed. When these calli are subjected to growth in cytokinin medium, somatic embryos are formed. They are circular, elongated,
Single cell culture
• As stated earlier, cells derived from a single cell through mitosis constitute a clone and the process of obtaining clones is called cloning (asexual progeny of a single individual make up.
Introduction to plant tissue culture...
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It allows for clonal propagation, production of secondary metabolites, induction of genetic variability, and regeneration of plants from somatic embryos or protoplasts. Key applications of plant tissue culture include micropropagation, production of pharmaceuticals, haploid production, somatic hybridization, transgenic plant production, germplasm conservation, breaking seed dormancy, and biomass energy production.
Similar to micropropogation (20)
Bio 130 tissue culture --intro lecture dev strategies part i tissue cultue 2...
Bio 130 tissue culture --intro lecture dev strategies part i tissue cultue 2...
Plant Tissue Culture Technique and its applications
Plant Tissue Culture Technique and its applications
Plant Tissue Culture Technique and its applications
Plant Tissue Culture Technique and its applications
Different types of Explants in Tissue Culture.pptx
Different types of Explants in Tissue Culture.pptx
Recently uploaded
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...Nguyen Thanh Tu Collection
https://app.box.com/s/tacvl9ekroe9hqupdnjruiypvm9rdaneGender and Mental Health - Counselling and Family Therapy Applications and In...
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Level 3 NCEA - NZ: A Nation In the Making 1872 - 1900 SML.ppt
The History of NZ 1870-1900.
Making of a Nation.
From the NZ Wars to Liberals,
Richard Seddon, George Grey,
Social Laboratory, New Zealand,
Confiscations, Kotahitanga, Kingitanga, Parliament, Suffrage, Repudiation, Economic Change, Agriculture, Gold Mining, Timber, Flax, Sheep, Dairying,
Leveraging Generative AI to Drive Nonprofit Innovation
In this webinar, participants learned how to utilize Generative AI to streamline operations and elevate member engagement. Amazon Web Service experts provided a customer specific use cases and dived into low/no-code tools that are quick and easy to deploy through Amazon Web Service (AWS.)
Chapter wise All Notes of First year Basic Civil Engineering.pptx
Chapter wise All Notes of First year Basic Civil Engineering
Syllabus
Chapter-1
Introduction to objective, scope and outcome the subject
Chapter 2
Introduction: Scope and Specialization of Civil Engineering, Role of civil Engineer in Society, Impact of infrastructural development on economy of country.
Chapter 3
Surveying: Object Principles & Types of Surveying; Site Plans, Plans & Maps; Scales & Unit of different Measurements.
Linear Measurements: Instruments used. Linear Measurement by Tape, Ranging out Survey Lines and overcoming Obstructions; Measurements on sloping ground; Tape corrections, conventional symbols. Angular Measurements: Instruments used; Introduction to Compass Surveying, Bearings and Longitude & Latitude of a Line, Introduction to total station.
Levelling: Instrument used Object of levelling, Methods of levelling in brief, and Contour maps.
Chapter 4
Buildings: Selection of site for Buildings, Layout of Building Plan, Types of buildings, Plinth area, carpet area, floor space index, Introduction to building byelaws, concept of sun light & ventilation. Components of Buildings & their functions, Basic concept of R.C.C., Introduction to types of foundation
Chapter 5
Transportation: Introduction to Transportation Engineering; Traffic and Road Safety: Types and Characteristics of Various Modes of Transportation; Various Road Traffic Signs, Causes of Accidents and Road Safety Measures.
Chapter 6
Environmental Engineering: Environmental Pollution, Environmental Acts and Regulations, Functional Concepts of Ecology, Basics of Species, Biodiversity, Ecosystem, Hydrological Cycle; Chemical Cycles: Carbon, Nitrogen & Phosphorus; Energy Flow in Ecosystems.
Water Pollution: Water Quality standards, Introduction to Treatment & Disposal of Waste Water. Reuse and Saving of Water, Rain Water Harvesting. Solid Waste Management: Classification of Solid Waste, Collection, Transportation and Disposal of Solid. Recycling of Solid Waste: Energy Recovery, Sanitary Landfill, On-Site Sanitation. Air & Noise Pollution: Primary and Secondary air pollutants, Harmful effects of Air Pollution, Control of Air Pollution. . Noise Pollution Harmful Effects of noise pollution, control of noise pollution, Global warming & Climate Change, Ozone depletion, Greenhouse effect
Text Books:
1. Palancharmy, Basic Civil Engineering, McGraw Hill publishers.
2. Satheesh Gopi, Basic Civil Engineering, Pearson Publishers.
3. Ketki Rangwala Dalal, Essentials of Civil Engineering, Charotar Publishing House.
4. BCP, Surveying volume 1
Educational Technology in the Health Sciences
Plenary presentation at the NTTC Inter-university Workshop, 18 June 2024, Manila Prince Hotel.
How to Predict Vendor Bill Product in Odoo 17
This slide will guide us through the process of predicting vendor bill products based on previous purchases from the vendor in Odoo 17.
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Aberdeen
Wound healing PPT
This document provides an overview of wound healing, its functions, stages, mechanisms, factors affecting it, and complications.
A wound is a break in the integrity of the skin or tissues, which may be associated with disruption of the structure and function.
Healing is the body’s response to injury in an attempt to restore normal structure and functions.
Healing can occur in two ways: Regeneration and Repair
There are 4 phases of wound healing: hemostasis, inflammation, proliferation, and remodeling. This document also describes the mechanism of wound healing. Factors that affect healing include infection, uncontrolled diabetes, poor nutrition, age, anemia, the presence of foreign bodies, etc.
Complications of wound healing like infection, hyperpigmentation of scar, contractures, and keloid formation.
Bossa N’ Roll Records by Ismael Vazquez.
Bossa N Roll Records presentation by Izzy Vazquez for Music Retail and Distribution class at Full Sail University
Recently uploaded (20)
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
spot a liar (Haiqa 146).pptx Technical writhing and presentation skills
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
BÀI TẬP BỔ TRỢ TIẾNG ANH LỚP 9 CẢ NĂM - GLOBAL SUCCESS - NĂM HỌC 2024-2025 - ...
Gender and Mental Health - Counselling and Family Therapy Applications and In...
Gender and Mental Health - Counselling and Family Therapy Applications and In...
SWOT analysis in the project Keeping the Memory @live.pptx
SWOT analysis in the project Keeping the Memory @live.pptx
Level 3 NCEA - NZ: A Nation In the Making 1872 - 1900 SML.ppt
Level 3 NCEA - NZ: A Nation In the Making 1872 - 1900 SML.ppt
Leveraging Generative AI to Drive Nonprofit Innovation
Leveraging Generative AI to Drive Nonprofit Innovation
NEWSPAPERS - QUESTION 1 - REVISION POWERPOINT.pptx
NEWSPAPERS - QUESTION 1 - REVISION POWERPOINT.pptx
Chapter wise All Notes of First year Basic Civil Engineering.pptx
Chapter wise All Notes of First year Basic Civil Engineering.pptx
Juneteenth Freedom Day 2024 David Douglas School District
Juneteenth Freedom Day 2024 David Douglas School District
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
micropropogation
- 1. Micropropagation “… the art and science of multiplying plants in vitro.”
- 2. Clone Genetically identical assemblage of individuals propagated entirely by vegetative means from a single plant.
- 3. Explant Cell, tissue or organ of a plant that is used to start in vitro cultures.
- 4. Axillary shoot proliferation Growth of axillary buds stimulated by cytokinin treatment; shoots arise mostly from pre-existing meristems.
- 5. Shoot proliferating culture of ‘Hally Jolivette’ cherry
- 7. Fungal contamination in rhododendron culture
- 8. Rhododendron cultures in the lab of Herman Losely & Son Nursery, Perry, OH
- 9. Amelanchier laevis transplants and field planting.
- 10. Micropropagation Advantages • From one to many propagules rapidly • Multiplication in controlled lab conditions • Continuous propagation year round • Potential for disease-free propagules
- 11. Conventional Propagation Advantages • Equipment costs minimal • Experience can substitute for technical expertise • Costs per unit are minimal • Potential for disease-free propagules • Specialized techniques for growth control (grafting onto dwarfing rootstocks)
- 12. Micropropagation Limitations • Equipment/facility intensive operation • Technical expertise in management positions • Protocols not optimized for all species • Liners may not fit industry standard • Propagules may be too expensive
- 13. Micropropagation Applications • Rapid build up of stock of new, superior variety • Elimination of endophytic disease • Better suited to morphology/growth characteristics (few offshoots/sprouts; date palms, ferns, nandinas) • Propagules have enhanced growth features (multibranched character; Ficus, Syngonium)
- 14. Shoot organogenesis Adventitious meristems differentiate on leaf, stem, or floral tissue.
- 15. African violet culture started from leaf section.
- 17. Begonia x chiemanta ‘Emma’ at various stages in micropropagation.
- 18. Somatic Embryogenesis Stimulation of callus or suspension cells to undergo a developmental pathway that mimics the development of the zygotic embryo.
- 20. Somatic Hybridization Fusion of protoplasts from different genotypes to bring about genetic recombination.
- 27. Somaclonal variation Heritable genetic variation that arises in plants produced through tissue culture.
- 33. Chimera Plant composed of two genotypes growing adjacent to one another in all (or part) of the plant body.
- 34. Inflorescence culture The inflorescence of many plants is a modified branching system. Vegetative meristems in the inflorescence may be stimulated to proliferate in vitro. bract flower bud peduncle axil