Plant tissue culture techniques allow for the growth and development of plant cells, tissues, organs, or protoplasts in sterile conditions on nutrient media. There are several types of in vitro culture including callus culture, organ culture, and somatic embryogenesis. Plant cells have the properties of totipotency, dedifferentiation, and competency which allow regeneration of whole plants from single cells. Tissue culture is important for applications such as crop improvement, mass propagation, and germplasm conservation.
This document discusses in vitro plant breeding techniques. It describes how plant cells, tissues, and organs can be cultured under controlled conditions in glass or plastic vessels with defined growth media. Plant cells have three key abilities - totipotency, dedifferentiation, and competency - that allow regeneration of whole plants in tissue culture. The ratio of auxin and cytokinin plant hormones can determine whether roots or shoots develop. Somatic embryogenesis is described as the formation of embryo-like structures from somatic cells that can develop into whole plants similarly to zygotic embryos.
This document discusses in vitro plant breeding techniques. It describes in vitro culture as the growth and development of plant tissues, cells, or protoplasts under controlled artificial conditions using glass or plastic vessels. The key abilities of plants that allow for in vitro culture are totipotency, dedifferentiation, and competency. Important factors for successful in vitro culture include growth media, environmental conditions, and the explant source. The document outlines various types of in vitro culture including seed, embryo, organ, callus, cell suspension, and protoplast cultures. It also describes plant regeneration pathways such as microcutting, organogenesis, and somatic embryogenesis.
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. Key factors include the growth media, environmental conditions, and the explant source tissue. Plant cells have the abilities of totipotency, dedifferentiation, and competency. Tissue culture is used for micropropagation, genetic modification, pathogen elimination, and more. Common types include callus, organ, and embryo culture. Somatic embryogenesis and organogenesis allow regeneration of whole plants. Tissue culture can generate somaclonal variation through genetic or epigenetic changes.
micropropagation- a very useful technology in plant tissue culture.YoGeshSharma834784
Plant tissue culture, also known as micropropagation, involves culturing plant cells, tissues or organs on nutrient media under sterile conditions. The ratio of two key hormones, auxin and cytokinin, determines whether roots or shoots develop. Micropropagation is used for clonal propagation of plants, germplasm preservation, studying somaclonal variation, embryo culture, haploid production, and in vitro hybridization. It allows for the rapid, mass production of genetically identical plantlets while providing benefits such as being disease-free.
This document discusses plant tissue culture and micropropagation. It describes how plant cells, tissues or organs can be cultured in vitro on nutrient media to rapidly multiply stock plant materials. Two key plant hormones, auxin and cytokinin, influence shoot versus root development. Micropropagation is used for clonal propagation, germplasm preservation, and producing disease-free plants. The process involves selection of explants, establishment of sterile culture, multiplication of shoots, rooting, and acclimatization. Organogenesis and somatic embryogenesis are two regeneration pathways used.
Clonal propagation invitro is called micropropagation.In micropropagation, apical meristem is cultivated. so this technique is also known as meristem culture or mericlones.Webber used the word ‘clone’for first time to apply for cultivated plants that were propagated vegetatively.It indicates that plants grown from such vegetative parts are not individuals in the ordinary sense, but are simply transplanted parts of the same individual and such plants are identical.
Tissue culture is the process of growing plant cells, tissues or organs in an artificial, sterile environment. It involves removing plant cells and placing them in a nutrient solution supplemented with hormones, vitamins and minerals. Key requirements include using appropriate explant tissue, a suitable growth medium, aseptic conditions, and growth regulators. The document outlines various tissue culture techniques such as micropropagation, callus culture, somatic embryogenesis, organogenesis, and anther/pollen/ovule/ovary culture. Factors affecting successful tissue culture include the plant genotype, explant source, nutrient composition of the growth medium, and control of environmental factors like light, temperature and sterility.
1. Somatic embryogenesis is a process where embryos are formed from somatic plant cells, such as leaf cells, that are not normally involved in embryo formation. 2. These somatic embryos develop through similar stages as zygotic embryos, including globular, heart-shaped, torpedo-shaped, and cotyledonary stages. 3. Somatic embryogenesis can occur through direct embryogenesis from explant tissue or indirect embryogenesis which involves first forming a callus that then develops embryos.
This document discusses in vitro plant breeding techniques. It describes how plant cells, tissues, and organs can be cultured under controlled conditions in glass or plastic vessels with defined growth media. Plant cells have three key abilities - totipotency, dedifferentiation, and competency - that allow regeneration of whole plants in tissue culture. The ratio of auxin and cytokinin plant hormones can determine whether roots or shoots develop. Somatic embryogenesis is described as the formation of embryo-like structures from somatic cells that can develop into whole plants similarly to zygotic embryos.
This document discusses in vitro plant breeding techniques. It describes in vitro culture as the growth and development of plant tissues, cells, or protoplasts under controlled artificial conditions using glass or plastic vessels. The key abilities of plants that allow for in vitro culture are totipotency, dedifferentiation, and competency. Important factors for successful in vitro culture include growth media, environmental conditions, and the explant source. The document outlines various types of in vitro culture including seed, embryo, organ, callus, cell suspension, and protoplast cultures. It also describes plant regeneration pathways such as microcutting, organogenesis, and somatic embryogenesis.
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. Key factors include the growth media, environmental conditions, and the explant source tissue. Plant cells have the abilities of totipotency, dedifferentiation, and competency. Tissue culture is used for micropropagation, genetic modification, pathogen elimination, and more. Common types include callus, organ, and embryo culture. Somatic embryogenesis and organogenesis allow regeneration of whole plants. Tissue culture can generate somaclonal variation through genetic or epigenetic changes.
micropropagation- a very useful technology in plant tissue culture.YoGeshSharma834784
Plant tissue culture, also known as micropropagation, involves culturing plant cells, tissues or organs on nutrient media under sterile conditions. The ratio of two key hormones, auxin and cytokinin, determines whether roots or shoots develop. Micropropagation is used for clonal propagation of plants, germplasm preservation, studying somaclonal variation, embryo culture, haploid production, and in vitro hybridization. It allows for the rapid, mass production of genetically identical plantlets while providing benefits such as being disease-free.
This document discusses plant tissue culture and micropropagation. It describes how plant cells, tissues or organs can be cultured in vitro on nutrient media to rapidly multiply stock plant materials. Two key plant hormones, auxin and cytokinin, influence shoot versus root development. Micropropagation is used for clonal propagation, germplasm preservation, and producing disease-free plants. The process involves selection of explants, establishment of sterile culture, multiplication of shoots, rooting, and acclimatization. Organogenesis and somatic embryogenesis are two regeneration pathways used.
Clonal propagation invitro is called micropropagation.In micropropagation, apical meristem is cultivated. so this technique is also known as meristem culture or mericlones.Webber used the word ‘clone’for first time to apply for cultivated plants that were propagated vegetatively.It indicates that plants grown from such vegetative parts are not individuals in the ordinary sense, but are simply transplanted parts of the same individual and such plants are identical.
Tissue culture is the process of growing plant cells, tissues or organs in an artificial, sterile environment. It involves removing plant cells and placing them in a nutrient solution supplemented with hormones, vitamins and minerals. Key requirements include using appropriate explant tissue, a suitable growth medium, aseptic conditions, and growth regulators. The document outlines various tissue culture techniques such as micropropagation, callus culture, somatic embryogenesis, organogenesis, and anther/pollen/ovule/ovary culture. Factors affecting successful tissue culture include the plant genotype, explant source, nutrient composition of the growth medium, and control of environmental factors like light, temperature and sterility.
1. Somatic embryogenesis is a process where embryos are formed from somatic plant cells, such as leaf cells, that are not normally involved in embryo formation. 2. These somatic embryos develop through similar stages as zygotic embryos, including globular, heart-shaped, torpedo-shaped, and cotyledonary stages. 3. Somatic embryogenesis can occur through direct embryogenesis from explant tissue or indirect embryogenesis which involves first forming a callus that then develops embryos.
Micropropagation, or in vitro clonal propagation, allows for the rapid multiplication of plant materials to produce many progeny plants using modern tissue culture methods. Clonal propagation is the asexual reproduction of genetically identical individuals. Micropropagation involves selecting plant material, sterilizing explants, culturing them on growth media, and multiplying shoots through successive transfers. Shoots are then rooted and acclimatized. The process uses controlled conditions and growth regulators to stimulate organogenesis or somatic embryogenesis. Micropropagation has applications for commercial scale propagation of new varieties, eliminating diseases, and cloning plants that are difficult to propagate conventionally.
MEDICINAL PLANT BIOTECHNOLOGY UNIT 2, MPG, SEM 2. NOTES Different tissue culture techniques: Organogenesis and embryogenesis, synthetic seed and monoclonal variation
Protoplast fusion, Hairy root multiple shoot cultures and their applications.
Micro propagation of medicinal and aromatic plants.
Sterilization methods involved in tissue culture, gene transfer in plants and their applications.
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. The key hormones auxin and cytokinin play important roles in differentiation. Tissue culture applications include micropropagation, germplasm preservation, haploid production, and genetic engineering. Important techniques include somatic embryogenesis, organogenesis, microcutting, anther/microspore culture, protoplast culture, and callus and cell suspension culture.
Micropropagation is the process of rapidly multiplying plant materials in an aseptic laboratory environment. It involves culturing small pieces of plant tissue on nutrient media containing hormones and sugars. The ratio of auxin and cytokinin hormones determines whether shoots or roots develop. Micropropagation has several advantages over traditional propagation methods, including producing many identical clones of plants that are disease-free and genetically uniform. The process involves initiation, multiplication, rooting, and transfer to soil stages. Common micropropagation techniques are meristem culture, callus culture, and embryogenesis.
Plant tissue culture involves growing plant cells, tissues, organs, or whole plants in vitro on a nutrient medium under sterile conditions. It allows for mass propagation of plant materials, rapid plant breeding through selection of variants, and genetic manipulation. The key principles involve using plant hormones like auxin and cytokinin to induce cell differentiation and regeneration into whole plants. Advantages include rapid multiplication, disease elimination, genetic transformation, and conservation of endangered species.
Tissue culture involves growing plant cells, tissues or organs in a nutrient medium under sterile conditions. It has several applications in agriculture, pharmacy and research. The document discusses the history, types (callus, suspension, organ and protoplast cultures), factors affecting tissue culture, and advantages and disadvantages. The types of tissue culture described in more detail are callus culture, which produces an unorganized cell mass, and suspension culture, which consists of freely growing single cells or cell clusters in liquid medium.
1. The document discusses various techniques of in vitro plant tissue culture including organ culture, seed culture, meristem culture, embryo culture, ovary culture, anther culture, callus culture, cell suspension culture, and protoplast culture.
2. Key steps in in vitro culture include isolation of explant tissues, regeneration and callus formation, embryogenesis, and organogenesis.
3. Applications of these techniques include virus-free plant production, increasing seed germination efficiency, producing somatic embryos and haploid plants, enabling single cell studies, and facilitating somatic hybridization.
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. Some key points:
- Callus culture involves growing an unorganized cell mass (callus) from explants on auxin-cytokinin media, which can then be used for micropropagation.
- Cell suspension culture breaks up callus into single cells that grow in liquid media, allowing for easier scaling up than callus.
- Micropropagation is the process of rapidly multiplying plant materials like shoots, roots or embryos in culture to produce many clonal copies of plant materials like orchids or strawberries.
- Protoplast isolation and culture allows the transfer of genes directly into plant cells without the
Plant tissue culture involves growing plant cells, tissues, or organs in sterile conditions on a nutrient medium. It allows for mass production of plant clones and regeneration of whole plants from modified plant cells. Key aspects of plant tissue culture include using explants, maintaining sterile conditions, and promoting cell differentiation and regeneration into whole plants using techniques like micropropagation and somatic embryogenesis. Success requires selecting the right tissue culture type and optimizing factors like media, light, and temperature for the specific plant.
Somatic embryogenesis is the process where embryos form from somatic (non-reproductive) plant cells in vitro. It is an important biotechnological tool that allows for clonal propagation, genetic transformation, and other applications. The first observation of somatic embryogenesis was in carrot cells in 1958. Somatic embryogenesis occurs through direct or indirect pathways and involves induction, development, and maturation stages. It has advantages over zygotic embryogenesis like a higher propagation rate and applications in synthetic seed production and genetic engineering.
A process where an embryo is derived from a single somatic cell or group of somatic cells. Somatic embryos (SEs) are formed from plant cells that are not normally involved in embryo formation.
Embryos formed by somatic embryogenesis are called Embryoids.
The process was discovered for the first time in Daucas carota L. (carrot) by Steward (1958), Reinert (1959).
Plant tissue culture is the process of growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It has many applications like germplasm preservation of endangered plants, producing disease-free plants through micropropagation, and creating novel hybrids through protoplast fusion and somatic hybridization. However, issues remain like genetic instability of hybrids and lack of efficient selection methods. Overall, tissue culture is a valuable biotechnology tool with potential for crop improvement and conservation efforts.
Plant tissue culture is the process of growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It has many applications like germplasm preservation of endangered plants, genetic improvement of crops, and production of secondary metabolites. The basic steps include selection of explant, initiation of culture on growth media, multiplication through cell division, and rooting and transfer to soil. Technologies such as micropropagation, somatic hybridization, and cryopreservation have been commercialized for mass propagation of crops. However, issues remain regarding genetic stability, selection of fused products, and regeneration efficiency.
1. Micropropagation is a process of rapidly multiplying plant materials using modern tissue culture methods. It involves 5 main stages - selection of stock plants, initiation and establishment of culture, multiplication of shoots, rooting of shoots, and establishment of plantlets.
2. There are 4 main approaches to micropropagation - multiplication by axillary buds/apical shoots, multiplication by adventitious shoots, organogenesis, and somatic embryogenesis. Each approach utilizes specific plant tissues and culture conditions.
3. Micropropagation has many applications including high rate propagation, production of disease-free plants, and generation of genetically modified plants. However, it also has disadvantages such as contamination, genetic variability, and vitrification of shoots.
Plant tissue culture is a technique where plant cells, tissues, or organs are grown in an artificial nutrient medium under sterile conditions. Haberlandt in 1902 first attempted to culture plant tissues in vitro and is considered the father of plant tissue culture. Important milestones in the history of plant tissue culture include the development of MS medium by Murashige and Skoog in 1962 and the first transgenic plant created in 1983. Common types of plant tissue culture include callus culture, suspension culture, organ culture, and meristem culture. Tissue culture has many applications in crop improvement including micropropagation, breeding, and production of secondary metabolites.
Plant biotechnology involves manipulating plants through genetic engineering techniques. It has applications in agriculture, medicine, and environmental protection. Key techniques include plant tissue culture, which grows plant tissues in sterile conditions, and genetic transformation, which transfers genes into plants. Plant tissue culture is used for micropropagation of plants, haploid production, and protoplast fusion experiments. Micropropagation allows for mass production of genetically identical plant clones through techniques like shoot culture, organogenesis, and somatic embryogenesis.
The document discusses organogenesis, which is the development of adventitious organs or primordial from undifferentiated plant cell mass through differentiation. It describes the process, including dedifferentiation and redifferentiation stages. There are two types of organogenesis - direct organogenesis which does not involve callus formation, and indirect organogenesis which involves callus formation. Organogenesis is used in plant tissue culture to regenerate plants through shoot or root cultures and is influenced by factors like explant source and size, plant growth regulators, and culture conditions. It has commercial applications in micropropagation of plants.
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
Micropropagation, or in vitro clonal propagation, allows for the rapid multiplication of plant materials to produce many progeny plants using modern tissue culture methods. Clonal propagation is the asexual reproduction of genetically identical individuals. Micropropagation involves selecting plant material, sterilizing explants, culturing them on growth media, and multiplying shoots through successive transfers. Shoots are then rooted and acclimatized. The process uses controlled conditions and growth regulators to stimulate organogenesis or somatic embryogenesis. Micropropagation has applications for commercial scale propagation of new varieties, eliminating diseases, and cloning plants that are difficult to propagate conventionally.
MEDICINAL PLANT BIOTECHNOLOGY UNIT 2, MPG, SEM 2. NOTES Different tissue culture techniques: Organogenesis and embryogenesis, synthetic seed and monoclonal variation
Protoplast fusion, Hairy root multiple shoot cultures and their applications.
Micro propagation of medicinal and aromatic plants.
Sterilization methods involved in tissue culture, gene transfer in plants and their applications.
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. The key hormones auxin and cytokinin play important roles in differentiation. Tissue culture applications include micropropagation, germplasm preservation, haploid production, and genetic engineering. Important techniques include somatic embryogenesis, organogenesis, microcutting, anther/microspore culture, protoplast culture, and callus and cell suspension culture.
Micropropagation is the process of rapidly multiplying plant materials in an aseptic laboratory environment. It involves culturing small pieces of plant tissue on nutrient media containing hormones and sugars. The ratio of auxin and cytokinin hormones determines whether shoots or roots develop. Micropropagation has several advantages over traditional propagation methods, including producing many identical clones of plants that are disease-free and genetically uniform. The process involves initiation, multiplication, rooting, and transfer to soil stages. Common micropropagation techniques are meristem culture, callus culture, and embryogenesis.
Plant tissue culture involves growing plant cells, tissues, organs, or whole plants in vitro on a nutrient medium under sterile conditions. It allows for mass propagation of plant materials, rapid plant breeding through selection of variants, and genetic manipulation. The key principles involve using plant hormones like auxin and cytokinin to induce cell differentiation and regeneration into whole plants. Advantages include rapid multiplication, disease elimination, genetic transformation, and conservation of endangered species.
Tissue culture involves growing plant cells, tissues or organs in a nutrient medium under sterile conditions. It has several applications in agriculture, pharmacy and research. The document discusses the history, types (callus, suspension, organ and protoplast cultures), factors affecting tissue culture, and advantages and disadvantages. The types of tissue culture described in more detail are callus culture, which produces an unorganized cell mass, and suspension culture, which consists of freely growing single cells or cell clusters in liquid medium.
1. The document discusses various techniques of in vitro plant tissue culture including organ culture, seed culture, meristem culture, embryo culture, ovary culture, anther culture, callus culture, cell suspension culture, and protoplast culture.
2. Key steps in in vitro culture include isolation of explant tissues, regeneration and callus formation, embryogenesis, and organogenesis.
3. Applications of these techniques include virus-free plant production, increasing seed germination efficiency, producing somatic embryos and haploid plants, enabling single cell studies, and facilitating somatic hybridization.
Plant tissue culture involves growing plant cells, tissues or organs in sterile conditions on nutrient media. Some key points:
- Callus culture involves growing an unorganized cell mass (callus) from explants on auxin-cytokinin media, which can then be used for micropropagation.
- Cell suspension culture breaks up callus into single cells that grow in liquid media, allowing for easier scaling up than callus.
- Micropropagation is the process of rapidly multiplying plant materials like shoots, roots or embryos in culture to produce many clonal copies of plant materials like orchids or strawberries.
- Protoplast isolation and culture allows the transfer of genes directly into plant cells without the
Plant tissue culture involves growing plant cells, tissues, or organs in sterile conditions on a nutrient medium. It allows for mass production of plant clones and regeneration of whole plants from modified plant cells. Key aspects of plant tissue culture include using explants, maintaining sterile conditions, and promoting cell differentiation and regeneration into whole plants using techniques like micropropagation and somatic embryogenesis. Success requires selecting the right tissue culture type and optimizing factors like media, light, and temperature for the specific plant.
Somatic embryogenesis is the process where embryos form from somatic (non-reproductive) plant cells in vitro. It is an important biotechnological tool that allows for clonal propagation, genetic transformation, and other applications. The first observation of somatic embryogenesis was in carrot cells in 1958. Somatic embryogenesis occurs through direct or indirect pathways and involves induction, development, and maturation stages. It has advantages over zygotic embryogenesis like a higher propagation rate and applications in synthetic seed production and genetic engineering.
A process where an embryo is derived from a single somatic cell or group of somatic cells. Somatic embryos (SEs) are formed from plant cells that are not normally involved in embryo formation.
Embryos formed by somatic embryogenesis are called Embryoids.
The process was discovered for the first time in Daucas carota L. (carrot) by Steward (1958), Reinert (1959).
Plant tissue culture is the process of growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It has many applications like germplasm preservation of endangered plants, producing disease-free plants through micropropagation, and creating novel hybrids through protoplast fusion and somatic hybridization. However, issues remain like genetic instability of hybrids and lack of efficient selection methods. Overall, tissue culture is a valuable biotechnology tool with potential for crop improvement and conservation efforts.
Plant tissue culture is the process of growing plant cells, tissues or organs in sterile conditions on a nutrient medium. It has many applications like germplasm preservation of endangered plants, genetic improvement of crops, and production of secondary metabolites. The basic steps include selection of explant, initiation of culture on growth media, multiplication through cell division, and rooting and transfer to soil. Technologies such as micropropagation, somatic hybridization, and cryopreservation have been commercialized for mass propagation of crops. However, issues remain regarding genetic stability, selection of fused products, and regeneration efficiency.
1. Micropropagation is a process of rapidly multiplying plant materials using modern tissue culture methods. It involves 5 main stages - selection of stock plants, initiation and establishment of culture, multiplication of shoots, rooting of shoots, and establishment of plantlets.
2. There are 4 main approaches to micropropagation - multiplication by axillary buds/apical shoots, multiplication by adventitious shoots, organogenesis, and somatic embryogenesis. Each approach utilizes specific plant tissues and culture conditions.
3. Micropropagation has many applications including high rate propagation, production of disease-free plants, and generation of genetically modified plants. However, it also has disadvantages such as contamination, genetic variability, and vitrification of shoots.
Plant tissue culture is a technique where plant cells, tissues, or organs are grown in an artificial nutrient medium under sterile conditions. Haberlandt in 1902 first attempted to culture plant tissues in vitro and is considered the father of plant tissue culture. Important milestones in the history of plant tissue culture include the development of MS medium by Murashige and Skoog in 1962 and the first transgenic plant created in 1983. Common types of plant tissue culture include callus culture, suspension culture, organ culture, and meristem culture. Tissue culture has many applications in crop improvement including micropropagation, breeding, and production of secondary metabolites.
Plant biotechnology involves manipulating plants through genetic engineering techniques. It has applications in agriculture, medicine, and environmental protection. Key techniques include plant tissue culture, which grows plant tissues in sterile conditions, and genetic transformation, which transfers genes into plants. Plant tissue culture is used for micropropagation of plants, haploid production, and protoplast fusion experiments. Micropropagation allows for mass production of genetically identical plant clones through techniques like shoot culture, organogenesis, and somatic embryogenesis.
The document discusses organogenesis, which is the development of adventitious organs or primordial from undifferentiated plant cell mass through differentiation. It describes the process, including dedifferentiation and redifferentiation stages. There are two types of organogenesis - direct organogenesis which does not involve callus formation, and indirect organogenesis which involves callus formation. Organogenesis is used in plant tissue culture to regenerate plants through shoot or root cultures and is influenced by factors like explant source and size, plant growth regulators, and culture conditions. It has commercial applications in micropropagation of plants.
ESPP presentation to EU Waste Water Network, 4th June 2024 “EU policies driving nutrient removal and recycling
and the revised UWWTD (Urban Waste Water Treatment Directive)”
ANAMOLOUS SECONDARY GROWTH IN DICOT ROOTS.pptxRASHMI M G
Abnormal or anomalous secondary growth in plants. It defines secondary growth as an increase in plant girth due to vascular cambium or cork cambium. Anomalous secondary growth does not follow the normal pattern of a single vascular cambium producing xylem internally and phloem externally.
Current Ms word generated power point presentation covers major details about the micronuclei test. It's significance and assays to conduct it. It is used to detect the micronuclei formation inside the cells of nearly every multicellular organism. It's formation takes place during chromosomal sepration at metaphase.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
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11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
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https://www.etran.rs/2024/en/home-english/
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BREEDING METHODS FOR DISEASE RESISTANCE.pptxRASHMI M G
Plant breeding for disease resistance is a strategy to reduce crop losses caused by disease. Plants have an innate immune system that allows them to recognize pathogens and provide resistance. However, breeding for long-lasting resistance often involves combining multiple resistance genes
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
2. Plant Tissue Culture
The culture and maintenance of plant cells and organs
The culture of plant seeds, organs, tissues, cells, or protoplasts
on nutrient media under sterile conditions
The growth and development of plant seeds, organs, tissues,
cells or protoplasts on nutrient media under sterile (axenic)
conditions
The in vitro, aseptic plant culture for any purpose including
genetic transformation and other plant breeding objectives,
secondary product production, pathogen elimination or for
asexual (micropropagation) or sexual propagation
3.
4. Important Factors
• Growth Media
– Minerals, Growth factors, Carbon source, Hormones
• Environmental Factors
– Light, Temperature, Photoperiod, Sterility, Media
• Explant Source
– Usually, the younger, less differentiated explant, the better
for tissue culture
– Different species show differences in amenability to tissue
culture
– In many cases, different genotypes within a species will have
variable responses to tissue culture; response to somatic
embryogenesis has been transferred between melon cultivars
through sexual hybridization
5. Basis for Plant Tissue Culture
• Two Hormones Affect Plant Differentiation:
– Auxin: Stimulates Root Development
– Cytokinin: Stimulates Shoot Development
• Generally, the ratio of these two hormones can
determine plant development:
– Auxin ↓Cytokinin = Root Development
– Cytokinin ↓Auxin = Shoot Development
– Auxin = Cytokinin = Callus Development
6. Hormone Product Name Function in Plant Tissue Culture
Auxins Indole-3-Acetic Acid
Indole-3-Butyric Acid
Indole-3-Butyric Acid, Potassium Salt
-Naphthaleneacetic Acid
2,4-Dichlorophenoxyacetic Acid
p-Chlorophenoxyacetic acid
Picloram
Dicamba
Adventitous root formation (high concen)
Adventitious shoot formation (low concen)
Induction of somatic embryos
Cell Division
Callus formation and growth
Inhibition of axillary buds
Inhibition of root elongation
Cytokinins 6-Benzylaminopurine
6-,-Dimethylallylaminopurine (2iP)
Kinetin
Thidiazuron (TDZ)
N-(2-chloro-4-pyridyl)-N’Phenylurea
Zeatin
Zeatin Riboside
Adventitious shoot formation
Inhibition of root formation
Promotes cell division
Modulates callus initiation and growth
Stimulation of axillary’s bud breaking and growth
Inhibition of shoot elongation
Inhibition of leaf senescence
Gibberellins Gibberellic Acid Stimulates shoot elongation
Release seeds, embryos, and apical buds from dormancy
Inhibits adventitious root formation
Paclobutrazol and ancymidol inhibit gibberellin synthesis thus
resulting in shorter shoots, and promoting tuber, corm, and bulb
formation.
Abscisic Acid Abscisic Acid Stimulates bulb and tuber formation
Stimulates the maturation of embryos
Promotes the start of dormancy
Polyamines Putrescine
Spermidine
Promotes adventitious root formation
Promotes somatic embryogenesis
Promotes shoot formation
7. Control of in vitro culture
Cytokinin
Auxin
Leaf strip
Adventitious
Shoot
Root
Callus
9. 1. Environmental condition optimized (nutrition, light,
temperature).
2. Ability to give rise to callus, embryos, adventitious roots and
shoots.
3. Ability to grow as single cells (protoplasts, microspores,
suspension cultures).
4. Plant cells are totipotent, able to regenerate a whole plant.
Characteristic of Plant
Tissue Culture Techniques
10. Three Fundamental Abilities of Plants
Totipotency
The potential or inherent capacity of a plant cell to develop into
an entire plant if suitably stimulated.
It implies that all the information necessary for growth and
reproduction of the organism is contained in the cell
Dedifferentiation
Capacity of mature cells to return to meristematic condition and
development of a new growing point, follow by redifferentiation
which is the ability to reorganize into new organ
Competency
The endogenous potential of a given cells or tissue to develop in a
particular way
11. Why is tissue culture important?
Plant tissue culture has value in studies such as cell
biology, genetics, biochemistry, and many other
research areas
Crop Improvement
Seed Production – Plant Propagation Technique
Genetic material conservation
12. Types of In Vitro Culture
(explant based)
Culture of intact plants (seed and seedling culture)
Embryo culture (immature embryo culture)
Organ culture
Callus culture
Cell suspension culture
Protoplast culture
13. Seed culture
Growing seed aseptically in vitro on artificial media
Increasing efficiency of germination of seeds that are
difficult to germinate in vivo
Precocious germination by application of plant growth
regulators
Production of clean seedlings for explants or meristem
culture
14. Embryo culture
Growing embryo aseptically in vitro on artificial nutrient media
It is developed from the need to rescue embryos (embryo rescue)
from wide crosses where fertilization occurred, but embryo
development did not occur
It has been further developed for the production of plants from
embryos developed by non-sexual methods (haploid production
discussed later)
Overcoming embryo abortion due to incompatibility barriers
Overcoming seed dormancy and self-sterility of seeds
Shortening of breeding cycle
15. Organ culture
Any plant organ can serve as an explant to initiate
cultures
No. Organ Culture types
1. Shoot Shoot tip culture
2. Root Root culture
3. Leaf Leaf culture
4. Flower Anther/ovary culture
16. Shoot apical meristem culture
Production of virus free
germplasm
Mass production of
desirable genotypes
Facilitation of exchange
between locations
(production of clean
material)
Cryopreservation (cold
storage) or in vitro
conservation of
germplasm
18. Ovary or ovule culture
Production of haploid plants
A common explant for the initiation of somatic
embryogenic cultures
Overcoming abortion of embryos of wide hybrids at
very early stages of development due to incompatibility
barriers
In vitro fertilization for the production of distant
hybrids avoiding style and stigmatic incompatibility that
inhibits pollen germination and pollen tube growth
19. Anther and microspore culture
Production of haploid plants
Production of homozygous diploid lines
through chromosome doubling, thus reducing
the time required to produce inbred lines
Uncovering mutations or recessive phenotypes
20. Callus Culture
Callus:
An un-organised mass of cells
A tissue that develops in response to injury caused by physical or
chemical means
Most cells of which are differentiated although may be and are
often highly unorganized within the tissue
21. Cell suspension culture
When callus pieces are
agitated in a liquid
medium, they tend to
break up.
Suspensions are much
easier to bulk up than
callus since there is no
manual transfer or solid
support.
23. Protoplast
The living material of a plant or bacterial cell, including the
protoplasm and plasma membrane after the cell wall has been
removed.
24. Plant Regeneration Pathways
Organogenesis
Relies on the production of organs either directly from an
explant or callus structure
Somatic Embryogenesis
Embryo-like structures which can develop into whole plants in a
way that is similar to zygotic embryos are formed from somatic
cells
Existing Meristems (Microcutting)
Uses meristematic cells to regenerate whole plant.
(Source:Victor. et al., 2004)
25. Organogenesis
• The ability of non-
meristematic plant tissues to
form various organs de novo.
• The formation of
adventitious organs
• The production of roots,
shoots or leaves
• These organs may arise out
of pre-existing meristems or
out of differentiated cells
• This may involve a callus
intermediate but often occurs
without callus.
26. Steps in Organogenesis
1. Phytohormone Perception
2. Dedifferentiation of differentiated cells to
acquire competence.
3. Reentry of cells into the cell cycle
4. Organization of cell division to form specific
organs primordia in meristem
(Source:Victor. et al, 2004)
27.
28. Indirect organogenesis
Explant → Callus → Meristemoid → Primordium
• Dedifferentiation
– Less committed,
– More plastic developmental state
• Induction
– Cells become organogenically competent and fully
determined for primordia production
• Differentiation
30. Somatic Embryogenesis
• The formation of
adventitious embryos
• The production of
embryos from somatic or
“non-germ” cells.
• It usually involves a callus
intermediate stage which
can result in variation
among seedlings
31. Various terms for non-zygotic
embryos
Adventious embryos
Somatic embryos arising directly from other organs or
embryos.
Parthenogenetic embryos (apomixis)
Somatic embryos are formed by the unfertilized egg.
Androgenetic embryos
Somatic embryos are formed by the male gametophyte.
32. Somatic Embryogenesis and Organogenesis
• Both of these technologies can be used as
methods of micropropagation.
• It is not always desirable because they may not
always result in populations of identical plants.
• The most beneficial use of somatic
embryogenesis and organogenesis is in the
production of whole plants from a single cell (or
a few cells).
33. Somatic embryogenesis differs from
organogenesis
• Bipolar structure with a closed radicular end rather
than a monopolar structure.
• The embryo arises from a single cell and has no
vascular connection with the mother tissue.
34. Two routes to somatic embryogenesis
(Sharp et al., 1980)
• Direct embryogenesis
– Embryos initiate directly from explant in the absence
of callus formation.
• Indirect embryogenesis
– Callus from explant takes place from which embryos
are developed.
37. Induction
• Auxins required for induction
–Proembryogenic masses form
–2,4-D most used
–NAA, dicamba also used
38. Development
Auxin must be removed for embryo development
Continued use of auxin inhibits embryogenesis
Stages are similar to those of zygotic embryogenesis
– Globular
– Heart
– Torpedo
– Cotyledonary
– Germination (conversion)
39. Maturation
• Require complete maturation with apical
meristem, radicle, and cotyledons
• Often obtain repetitive embryony
• Storage protein production necessary
• Often require ABA for complete maturation
• ABA often required for normal embryo
morphology
– Fasciation
– Precocious germination
40. Germination
• May only obtain 3-5% germination
• Sucrose (10%), mannitol (4%) may be required
• Drying (desiccation)
– ABA levels decrease
– Woody plants
– Final moisture content 10-40%
• Chilling
– Decreases ABA levels
– Woody plants
41. Types of embryogenic cells
• Pre-embryogenic determined cells, PEDCs
– The cells are committed to embryonic development and need
only to be released. Such cells are found in embryonic tissue.
• Induced embryogenic determined cells, IEDCs
– In majority of cases embryogenesis is through indirect method.
– Specific growth regulator concentrations and/or cultural
conditions are required for initiation of callus and then
redetermination of these cells into the embryogenic pattern of
development.
42. Somatic embryogenesis as a means
of propagation is seldom used
High probability of mutations
The method is usually rather difficult.
Losing regenerative capacity become greater with
repeated subculture
Induction of embryogenesis is very difficult with many
plant species.
A deep dormancy often occurs with somatic
embryogenesis
44. Microcutting propagation
• It involves the production of shoots from pre-existing
meristems only.
• Requires breaking apical dominance
• This is a specialized form of organogenesis
45. Steps of Micropropagation
• Stage 0 – Selection & preparation of the mother plant
– sterilization of the plant tissue takes place
• Stage I - Initiation of culture
– explant placed into growth media
• Stage II - Multiplication
– explant transferred to shoot media; shoots can be constantly
divided
• Stage III - Rooting
– explant transferred to root media
• Stage IV - Transfer to soil
– explant returned to soil; hardened off