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 Canning is a method of
preserving food in which the
food contents are processed and
sealed in an airtight container.
 Canning provides shelf life
typically ranging from one to five
years, although under specific
circumstances it can be much
longer A freeze-dried canned
product, such as canned dried
lentils, , could last as long as 30
years in an edible state
 The canning process dates back to the
late 18th century in France when the
Emperor Napoleon Bonaparte, concerned
about keeping his armies fed,
 offered a cash prize to whoever could
develop a method of food preservation.
 Nicholas Appert conceived the idea of
preserving food in bottles, like wine.
 After 15 years of experimentation, he
realized if food is sufficiently heated and
sealed in an airtight container, it will not
spoil. No preservatives are necessary.
1. Packing the product into
the container.
2. Hermetically sealing the
container
3. Thermally processing for
product and the container
together.
4. Cooling
5. Storage.
The main steps in canning are:
1. Packing the product into the container.
2. Hermetically sealing the container. Hermetically sealed
container means a container that is designed and intended to
be secure against the entry of microorganisms.
› Sealing glass jars
› Sealing metal cans
3. Thermally processing the product and the container together.
 High-Acid foods( jams, jellies, sauerkraut, kimchi, pickles,
fermented vegetables,) are processed at 100° C in Water
bath canner
 Low-Acid foods( such as meat, poultry, fish and vegetable)
must be processed at 121° C in Pressure canner
High-Acid foods
 fruits,jams, jellies, sauerkraut,fermented
vegetables,lemon juice.
 100° C in water bath canner
 A boiling water bath can only heat the food
to the temperature of boiling water.
Because the acidity of the ingredients as
much as the heat of the processing that
safely preserves the food.
Low-Acid and
alkaline foods
 such as meat, fish and vegetable
 116 - 121° C in pressure canner
 heating the food in the jars to hotter than
the temperature of boiling water. The reason
for that is that although botulism bacteria is
killed at the temperature of boiling water,
botulism spores can survive that
temperature. The spores can be eliminated
by temperatures hotter than boiling water.
4. Cooling
cans is immersed in cold water Or let them to cool at room
temperature from 12-24 hours.
 As the temperature of the product drops, a vacuum
forms inside and pulls down the lid. This is often
accompanied by a popping sound and happens
within minutes after removing the jar from the
water bath canner.
5. Storage: Should be stored in a cool , dry, dark place,
Should not be stored where temperature extremes
exist, Should be used within one year for best
quality
 The spoilage of can could be due to biological or
chemical reasons or combination of both. The
biological spoilage is primarily due to microbial
growth. while chemical spoilage is due to
hydrogen produced due to reaction of acid in food
and iron on can.
 The degree of swelling can also be increased by
high summer temperature.
1.1. Hydrogen swell
 The chemical spoilage in most cases is due to production of
hydrogen gas produced in can
because of action of acid of food on iron of can
 Occur mainly in acidic foods (canned fruits). Not related to
fermentation or bacterial spoilage.
 Can show varying degree of bulging when opened odorless
burnable gas ..
 . It occurs due
a) Increased storage temperature.
b) Increased acidity of food
c) Presence of soluble sulfur and phosphorous compounds
d) lacquering of can at internal
surfaces
1.2 Sulphiding (Sulphur stinker spoilage):
 this spoilage cause discoloration of
inside of can with pink to dark
purple.
 Due to hydrogen sulfide formation
as a result of breakdown of
sulphur-containing proteins (liver,
kidney, tongue) by organisms of
colestidia groub (Cl. nigrificans
(sulphur stinker)) with the odor of
rotted egg.
 It may be accompanied with
blackening when H2S react with
steel base of tin forming iron
sulphide and may lead to pitting.
1.3- Rust and damage:
Rust is reddish brown ferric oxide
seen under label.
 Slight rust:pass for rapid consumption.
 Severe rust:condemned and rejected.

incipient spoilage leakage spoilage
inadequate thermal
processing
Thermophilic
Spoilage
due to storage at
high temperatures
Growth of acid-
tolerant spore
forming
microorganisms
 If food is held too long during
or between filling and closing
the containers and the delivery
of the thermal process .
 The microorganisms normally
may have sufficient time to
replicate and spoil the product.
 This microorganisms that grow
will be subsequently killed by
the thermal process
however,if sufficient growth
occurred to alter the product
characteristics ,the product
would be considere dspoiled.
 occur when there is an
opportunity for microorganisms to
enter the container.
 maintaining the hermetic seal is
essential to preventing leakage
spoilage.
 Leakage is generally due to
inadequately formed seams,
defective containers or closures,
cooling water contaminated with
large numbers of microorganisms,
container damage, rough handling
of processed containers, dirty can-
handling equipment, or a
combination of these
 Heat processes for canned foods are designed to destroy all
microorganisms of public health significance as well as
microorganisms of non-public health.
 “Inadequate thermal processing” indicates that the thermal
process that was applied to the product was insufficient to destroy
these organisms.
 An inadequate thermal process may occur for a number of reasons:
(1) The thermal process is not properly established.
(2) The time and/or temperature specified in the thermal process for
the product and specified container size is not used.
(3) some mechanical or personnel failure.
 this spoilage usually appearing as swollen containers.
 This spoilage caused by a single sporeforming type of organism.
 The spores of thermophilic bacteria usually have a
greater heat resistance than the spores of mesophilic
bacteria. Therefore, heat processes designed to kill
mesophilic bacterial spores are not adequate to destroy
thermophilic bacterial spores.
 it would be impractical to establish thermal processes to
destroy these organisms because the quality of the food
products would suffer greatly.
 Therefore, 1. products must be properly cooled after
thermal processing, 2.and held below 35◦C during
subsequent storage, in order to prevent thermophilic
spoilage.
 Thermophilic spoilage has economic significance, but not
public health significance, since there are no known
thermophilic foodborne pathogens.
 occur in product with a pH less than or equal to 4.6.
 Butyric acid anaerobes can be a problem in products
with a pH between 4.2 and 4.6.
 Heat-resistant molds have caused spoilage problems in
acid and acidified foods
(1) Mesophilic Aerobic
Sporeformers:
 Strains of Bacillus
 The presence of Bacillus species is
generally the result of improper
process application.
 Moderate heat resistance .
(2) Mesophilic Anaerobic
Sporeformers:
 primarily belong to the genus
Clostridium, and those of greatest
interest in foods
 fall into two main groups : C.
sporogenes (the proteolytic strains
of C. botulinum).. Clostridium
perfringens( the nonproteolytic
strains) .
(3) Putrefactive Anaerobes:
 Proteolytic mesophilic anaerobes may cause
spoilage of canned foods having a pH of 4.8 or
above
 Spoilage by these anaerobes is generally
characterized by gas production that swells
the container,are reduced product pH,and a
foul putrid odor,hence the generic name
“putrefactive anaerobes" .
(4) Aciduric Flat Sour Sporeformers
 facultative anaerobic sporeformers that produce
gas in spoiled products.
 The ends of spoiled cans remain flat
 the term “flat sour.” Spoiled products have an off-
flavor that has been described as “medicinal” or
“phenolic.”
 These organisms (e.g., Bacillus coagulans) have
caused spoilage in acid foods such as tomato
products
:(5) Thermophilic Flat Sour Sporeformers
 The organisms in this spoilage family generally grow between
40 and 90◦C, with optimum growth seen between 55 and 65◦C.
 The spores of these organisms have a very high heat resistance
than the thermal processes However it do not produce toxins
or infections in humans..
 The most common species of concern are Bacillus
stearothermophilus.
(6) Thermophilic Anaerobic Sporeformers
Sulfide “Stinkers
 Desulfotomaculum nigrificans (known as Clostridium
nigrificans) Is a thermophilic anaerobe
 produces hydrogen sulfide with its characteristic rotten egg
odor and discoloration
 seen as a problem in the sugar industry, spoiling
molasses on occasion.
 The interior of the can and the product blackens from
the interaction of the dissolved H2S with the iron in
the container
(7) Heat-Resistant Molds
 Most fungi have very limited heat resistance, except
for a group of fungi.
 Acidic and suger concentration as high as 70% can
permit mold growth
 The most isolated are from the genera Byssochlamys,
Neosartorya, and Talaromyces
 isolated mainly from fruit and fruit products such as
fruit juices
:(8) Non-sporeformers
 isolated in the examination of spoiled canned foods.
 When these oranisms are present, they indicate the
product was not processed or that post-process
contamination occurred.
 more common in underprocessed acid and acidified
foods where the thermal process is much less severe
than for low-acid foods.
 They include yeast, mold, Gram-positive bacteria such
and Gram-negative bacteria such as Enterobacter and
Pseudomonas.
 Can has swollen irregular appearance: Most
microorganisms produce gas when they grow. If they
grow in a canned food, the gas produced generally
cannot escape and causes the container to swell ,
providing visible evidence that the product inside
might be spoiled.
• if sufficient gas is produced, they may explode from
excessive internal pressure. Flexible or non-rigid
containers will appear “ballooned.”
 Can is leaky or corroded.
 Can is rusty
 Contents smell putrid or alcoholic
 Contents are discoloured
 Syrup looks turbid, cloudy, slimy or mouldy.
 The disease is caused by
C.botulinum
 which is a Gram positive,
motile, anaerobic, rod shapped
bacteria .
 Low pH (4.6), in the
temperature range of 20 to 45.
 Some strains produce botulinal
enterotoxins that cause severe
neuroparalytic condition known
as botulism.
 Eight types of botulinal
neurotoxins from A to H
 Human botulism is caused by
types A, B, E and rarely F
 The most common way of
getting botulism is either by
eating the contaminated foods
or drinking the contaminated
beverages.
 The patient may show blurred
or double vision, vertigo, dry
mouth, slurred speech,
drooping eyelids, difficulty in
swallowing and speaking,
muscles weakness, marked
fatigue and weakness
Vomiting, diarrhoea and
abdominal pain .
 Unless treated, mortality rate
may be very high which may
vary from 30 to 65 %
 The death may occur due to
respiratory and cardiac failure;
and recovery may be slow
microbiological spoilage of canned food

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microbiological spoilage of canned food

  • 1. Done by : Under supervision of :
  • 2.
  • 3.  Canning is a method of preserving food in which the food contents are processed and sealed in an airtight container.  Canning provides shelf life typically ranging from one to five years, although under specific circumstances it can be much longer A freeze-dried canned product, such as canned dried lentils, , could last as long as 30 years in an edible state
  • 4.  The canning process dates back to the late 18th century in France when the Emperor Napoleon Bonaparte, concerned about keeping his armies fed,  offered a cash prize to whoever could develop a method of food preservation.  Nicholas Appert conceived the idea of preserving food in bottles, like wine.  After 15 years of experimentation, he realized if food is sufficiently heated and sealed in an airtight container, it will not spoil. No preservatives are necessary.
  • 5. 1. Packing the product into the container. 2. Hermetically sealing the container 3. Thermally processing for product and the container together. 4. Cooling 5. Storage.
  • 6. The main steps in canning are: 1. Packing the product into the container. 2. Hermetically sealing the container. Hermetically sealed container means a container that is designed and intended to be secure against the entry of microorganisms. › Sealing glass jars › Sealing metal cans 3. Thermally processing the product and the container together.  High-Acid foods( jams, jellies, sauerkraut, kimchi, pickles, fermented vegetables,) are processed at 100° C in Water bath canner  Low-Acid foods( such as meat, poultry, fish and vegetable) must be processed at 121° C in Pressure canner
  • 7. High-Acid foods  fruits,jams, jellies, sauerkraut,fermented vegetables,lemon juice.  100° C in water bath canner  A boiling water bath can only heat the food to the temperature of boiling water. Because the acidity of the ingredients as much as the heat of the processing that safely preserves the food. Low-Acid and alkaline foods  such as meat, fish and vegetable  116 - 121° C in pressure canner  heating the food in the jars to hotter than the temperature of boiling water. The reason for that is that although botulism bacteria is killed at the temperature of boiling water, botulism spores can survive that temperature. The spores can be eliminated by temperatures hotter than boiling water.
  • 8. 4. Cooling cans is immersed in cold water Or let them to cool at room temperature from 12-24 hours.  As the temperature of the product drops, a vacuum forms inside and pulls down the lid. This is often accompanied by a popping sound and happens within minutes after removing the jar from the water bath canner. 5. Storage: Should be stored in a cool , dry, dark place, Should not be stored where temperature extremes exist, Should be used within one year for best quality
  • 9.
  • 10.  The spoilage of can could be due to biological or chemical reasons or combination of both. The biological spoilage is primarily due to microbial growth. while chemical spoilage is due to hydrogen produced due to reaction of acid in food and iron on can.  The degree of swelling can also be increased by high summer temperature.
  • 11.
  • 12. 1.1. Hydrogen swell  The chemical spoilage in most cases is due to production of hydrogen gas produced in can because of action of acid of food on iron of can  Occur mainly in acidic foods (canned fruits). Not related to fermentation or bacterial spoilage.  Can show varying degree of bulging when opened odorless burnable gas ..  . It occurs due a) Increased storage temperature. b) Increased acidity of food c) Presence of soluble sulfur and phosphorous compounds d) lacquering of can at internal surfaces
  • 13. 1.2 Sulphiding (Sulphur stinker spoilage):  this spoilage cause discoloration of inside of can with pink to dark purple.  Due to hydrogen sulfide formation as a result of breakdown of sulphur-containing proteins (liver, kidney, tongue) by organisms of colestidia groub (Cl. nigrificans (sulphur stinker)) with the odor of rotted egg.  It may be accompanied with blackening when H2S react with steel base of tin forming iron sulphide and may lead to pitting. 1.3- Rust and damage: Rust is reddish brown ferric oxide seen under label.  Slight rust:pass for rapid consumption.  Severe rust:condemned and rejected. 
  • 14. incipient spoilage leakage spoilage inadequate thermal processing Thermophilic Spoilage due to storage at high temperatures Growth of acid- tolerant spore forming microorganisms
  • 15.  If food is held too long during or between filling and closing the containers and the delivery of the thermal process .  The microorganisms normally may have sufficient time to replicate and spoil the product.  This microorganisms that grow will be subsequently killed by the thermal process however,if sufficient growth occurred to alter the product characteristics ,the product would be considere dspoiled.
  • 16.  occur when there is an opportunity for microorganisms to enter the container.  maintaining the hermetic seal is essential to preventing leakage spoilage.  Leakage is generally due to inadequately formed seams, defective containers or closures, cooling water contaminated with large numbers of microorganisms, container damage, rough handling of processed containers, dirty can- handling equipment, or a combination of these
  • 17.  Heat processes for canned foods are designed to destroy all microorganisms of public health significance as well as microorganisms of non-public health.  “Inadequate thermal processing” indicates that the thermal process that was applied to the product was insufficient to destroy these organisms.  An inadequate thermal process may occur for a number of reasons: (1) The thermal process is not properly established. (2) The time and/or temperature specified in the thermal process for the product and specified container size is not used. (3) some mechanical or personnel failure.  this spoilage usually appearing as swollen containers.  This spoilage caused by a single sporeforming type of organism.
  • 18.  The spores of thermophilic bacteria usually have a greater heat resistance than the spores of mesophilic bacteria. Therefore, heat processes designed to kill mesophilic bacterial spores are not adequate to destroy thermophilic bacterial spores.  it would be impractical to establish thermal processes to destroy these organisms because the quality of the food products would suffer greatly.  Therefore, 1. products must be properly cooled after thermal processing, 2.and held below 35◦C during subsequent storage, in order to prevent thermophilic spoilage.  Thermophilic spoilage has economic significance, but not public health significance, since there are no known thermophilic foodborne pathogens.
  • 19.  occur in product with a pH less than or equal to 4.6.  Butyric acid anaerobes can be a problem in products with a pH between 4.2 and 4.6.  Heat-resistant molds have caused spoilage problems in acid and acidified foods
  • 20.
  • 21. (1) Mesophilic Aerobic Sporeformers:  Strains of Bacillus  The presence of Bacillus species is generally the result of improper process application.  Moderate heat resistance . (2) Mesophilic Anaerobic Sporeformers:  primarily belong to the genus Clostridium, and those of greatest interest in foods  fall into two main groups : C. sporogenes (the proteolytic strains of C. botulinum).. Clostridium perfringens( the nonproteolytic strains) .
  • 22. (3) Putrefactive Anaerobes:  Proteolytic mesophilic anaerobes may cause spoilage of canned foods having a pH of 4.8 or above  Spoilage by these anaerobes is generally characterized by gas production that swells the container,are reduced product pH,and a foul putrid odor,hence the generic name “putrefactive anaerobes" . (4) Aciduric Flat Sour Sporeformers  facultative anaerobic sporeformers that produce gas in spoiled products.  The ends of spoiled cans remain flat  the term “flat sour.” Spoiled products have an off- flavor that has been described as “medicinal” or “phenolic.”  These organisms (e.g., Bacillus coagulans) have caused spoilage in acid foods such as tomato products
  • 23. :(5) Thermophilic Flat Sour Sporeformers  The organisms in this spoilage family generally grow between 40 and 90◦C, with optimum growth seen between 55 and 65◦C.  The spores of these organisms have a very high heat resistance than the thermal processes However it do not produce toxins or infections in humans..  The most common species of concern are Bacillus stearothermophilus. (6) Thermophilic Anaerobic Sporeformers Sulfide “Stinkers  Desulfotomaculum nigrificans (known as Clostridium nigrificans) Is a thermophilic anaerobe  produces hydrogen sulfide with its characteristic rotten egg odor and discoloration
  • 24.  seen as a problem in the sugar industry, spoiling molasses on occasion.  The interior of the can and the product blackens from the interaction of the dissolved H2S with the iron in the container (7) Heat-Resistant Molds  Most fungi have very limited heat resistance, except for a group of fungi.  Acidic and suger concentration as high as 70% can permit mold growth  The most isolated are from the genera Byssochlamys, Neosartorya, and Talaromyces  isolated mainly from fruit and fruit products such as fruit juices
  • 25. :(8) Non-sporeformers  isolated in the examination of spoiled canned foods.  When these oranisms are present, they indicate the product was not processed or that post-process contamination occurred.  more common in underprocessed acid and acidified foods where the thermal process is much less severe than for low-acid foods.  They include yeast, mold, Gram-positive bacteria such and Gram-negative bacteria such as Enterobacter and Pseudomonas.
  • 26.  Can has swollen irregular appearance: Most microorganisms produce gas when they grow. If they grow in a canned food, the gas produced generally cannot escape and causes the container to swell , providing visible evidence that the product inside might be spoiled. • if sufficient gas is produced, they may explode from excessive internal pressure. Flexible or non-rigid containers will appear “ballooned.”  Can is leaky or corroded.  Can is rusty  Contents smell putrid or alcoholic  Contents are discoloured  Syrup looks turbid, cloudy, slimy or mouldy.
  • 27.
  • 28.  The disease is caused by C.botulinum  which is a Gram positive, motile, anaerobic, rod shapped bacteria .  Low pH (4.6), in the temperature range of 20 to 45.  Some strains produce botulinal enterotoxins that cause severe neuroparalytic condition known as botulism.  Eight types of botulinal neurotoxins from A to H  Human botulism is caused by types A, B, E and rarely F
  • 29.  The most common way of getting botulism is either by eating the contaminated foods or drinking the contaminated beverages.  The patient may show blurred or double vision, vertigo, dry mouth, slurred speech, drooping eyelids, difficulty in swallowing and speaking, muscles weakness, marked fatigue and weakness Vomiting, diarrhoea and abdominal pain .  Unless treated, mortality rate may be very high which may vary from 30 to 65 %  The death may occur due to respiratory and cardiac failure; and recovery may be slow