This document summarizes the addition of 234 microsatellite marker loci to the Molecular Ecology Resources Database from 16 species. It provides the number of loci developed for each species, any other species the loci were tested in, and the accession numbers for the loci in both the Molecular Ecology Resources Database and GenBank. The authors responsible for developing each set of loci are listed in the final column of Table 1.
Detection and Subtype Identification of Blastocystis Isolates from Wastewater...gon0603
Presented during the 6th Asian-Pacific Organization for Cell Biology (APOCB) International Congress, EDSA Shangri-La, Manila, Philippines, 25 to 28 February 2011
Unicellular opisthokonts diversity and distribution along the European coast ...Javier del Campo
Unicellular opisthokonts include relevant eukaryotic lineages from an evolutionary and an ecological point of view. In our study we analyzed the 18S rDNA and rRNA V4 region of the unicellular opisthokonts (excluding fungi). Sequences were obtained by high-throughput methods from the European coast in the context of the BioMarKs project. We described the abundance and the distributions of the studied organisms in different sampling points and size fractions. Furthermore the ratio RNA:DNA allowed us to determine the most active groups. By using pysosequencing we had access to the less abundant and rare unicellular opisthokonts of the community. Using a curated reference database we properly placed the sequences retrieved in the opisthokonts tree, describing new diversity and identifying some groups not previously reported in marine environments. High-throughput sequencing techniques successfully increase our knowledge on the diversity of unicellular opisthokonts, its geographical distribution and possible ecological role.
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...Javier del Campo
Using Single Cell Sorting and Single Cell Genomics on marine surface water samples we were able to obtain and characterize 17 Chlorarachniophytes Single Amplified Genomes from both the heterotrophic and phototrophic fraction, including among them Minorisa minuta and the well known Bigelowella natans. The tiny uniflagellated Minorisa minuta stands up as one of the smallest bacterial grazers known to date. It has a worldwide planktonic distribution and accounts for 5% of heterotrophic protists communities in coastal waters. Moreover, it apparently represents the only heterotrophic representative within the Chlorarachniophytes, the single photosynthetic lineage within Rhizaria. Chlorarachniophytes are marine amoeboflagellated cercozoans that together with the Euglenophytes represent the two eukaryotic groups that acquired a chloroplast by secondary endosymbiosis with a green alga. The existence of this unpigmented protist could be another evidence indicating that the acquisition of the green chloroplast took place independently in both lineages. Nevertheless, this is not a definitive clue as the absence of chloroplast could also derive from a posterior loss of it. Therefore, the genomic analysis of Minorisa minuta and other basal heterotrophic Chlorarachniophytes retrieved in our study is of primary interest to study the transition to secondary plastid endosymbiosis.
Detection and Subtype Identification of Blastocystis Isolates from Wastewater...gon0603
Presented during the 6th Asian-Pacific Organization for Cell Biology (APOCB) International Congress, EDSA Shangri-La, Manila, Philippines, 25 to 28 February 2011
Unicellular opisthokonts diversity and distribution along the European coast ...Javier del Campo
Unicellular opisthokonts include relevant eukaryotic lineages from an evolutionary and an ecological point of view. In our study we analyzed the 18S rDNA and rRNA V4 region of the unicellular opisthokonts (excluding fungi). Sequences were obtained by high-throughput methods from the European coast in the context of the BioMarKs project. We described the abundance and the distributions of the studied organisms in different sampling points and size fractions. Furthermore the ratio RNA:DNA allowed us to determine the most active groups. By using pysosequencing we had access to the less abundant and rare unicellular opisthokonts of the community. Using a curated reference database we properly placed the sequences retrieved in the opisthokonts tree, describing new diversity and identifying some groups not previously reported in marine environments. High-throughput sequencing techniques successfully increase our knowledge on the diversity of unicellular opisthokonts, its geographical distribution and possible ecological role.
Minorisa minuta and the transition to secondary plastid endosymbiosis. A sing...Javier del Campo
Using Single Cell Sorting and Single Cell Genomics on marine surface water samples we were able to obtain and characterize 17 Chlorarachniophytes Single Amplified Genomes from both the heterotrophic and phototrophic fraction, including among them Minorisa minuta and the well known Bigelowella natans. The tiny uniflagellated Minorisa minuta stands up as one of the smallest bacterial grazers known to date. It has a worldwide planktonic distribution and accounts for 5% of heterotrophic protists communities in coastal waters. Moreover, it apparently represents the only heterotrophic representative within the Chlorarachniophytes, the single photosynthetic lineage within Rhizaria. Chlorarachniophytes are marine amoeboflagellated cercozoans that together with the Euglenophytes represent the two eukaryotic groups that acquired a chloroplast by secondary endosymbiosis with a green alga. The existence of this unpigmented protist could be another evidence indicating that the acquisition of the green chloroplast took place independently in both lineages. Nevertheless, this is not a definitive clue as the absence of chloroplast could also derive from a posterior loss of it. Therefore, the genomic analysis of Minorisa minuta and other basal heterotrophic Chlorarachniophytes retrieved in our study is of primary interest to study the transition to secondary plastid endosymbiosis.
Cyanobacteria, microcystins and cylindrospermopsin in public drinking supply ...M Carmo Oliveira
O Brasil tem um histórico de contaminações em reservatórios de águas destinadas ao abastecimento público por toxinas de cianobactérias. Essas toxinas, entre elas microcistinas e cilindropspermopsinas, causam sérios problemas ao homem. O estudo relata a ocorrência de MC e CYN em 10 reservatórios de abastecimento público do nordeste do Brasil. Devido à similaridade das comunidades de cianobactérias encontradas nos reservatórios e a presença de espécies potencialmente produtoras de toxinas, associada às MC e CYN, o estudo sugere que uma expansão geográfica possa ser favorecida por esses fatores.
Determination of acute toxicity and the effects of sub-acute concentrations o...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
Copper oxidenanoparticles have different industrial applications so it is inevitable that nanoparticulate products finally find their way into aquatic ecosystems. Nevertheless there is little information available about their effects on some of edible fish. The present study aims to determine the acute toxicity and evaluate the effect of two sub-acute concentrations (50 and 70% 96 h LC50) of CuO-NPs on some hematological and biochemical parameters of R. rutilus.
Materials and Methods:
225 healthy specimen of R. rutilus (mean weight 5.52±1.2 g; mean length 6.20±0.2 cm) were transported to the laboratory. In order to prepare the stock solution, CuO-NPs was dispersed in pure water with ultrasonication (50-60 kHz) for 15 min every day before dosing. At first, R. rutilus was exposed to CuO-NPs to determine the lethal concentration (LC50) value. Following acute test, fish were treated with sub-acute concentrations of CuO-NPs (50 and 70% 96 h-LC50 at) with one control group (no CuO-NPs) for a week to determine the changes in the level of some plasma hematological and biochemical parameters.
Results:
The 96 h-LC50 values of CuO-NPs was 2.19±0.003 mg/l. R. rutilus exhibited significantly lower RBC count, Hb and Hct values and a significant increase in the WBC numbers, MCH, MCHC and MCV indices (p<0.05).><0.05).
Conclusion:
These alterations indicate R. rutilus sensitivity to CuO-NPs and changes in blood parameters would be a useful tool for measurement early exposure to CuO nanoparticles.
Evolution & Fisheries: A Relationship?: Dr. Malin Pinskycoseenow
Dr. Malin Pinsky, Rutgers University, presented on his work with respect to the impacts of overfishing on genetic diversity in fishes at the January 9, 2014 STEM Educators' Series.
Metagenomics to Unlock the Biotechnological Potential of Marine Environments by Michele de Cássia Pereira e Silva in Examines in Marine C Biology & Oceanography
Atlas of Brazilian Snakes: Verified Point-Locality Maps to
Mitigate the Wallacean Shortfall in a Megadiverse Snake
Fauna
Authors: Nogueira, Cristiano C., Argôlo, Antonio J.S., Arzamendia,
Vanesa, Azevedo, Josué A., Barbo, Fausto E., et. al.
Source: South American Journal of Herpetology, 14(sp1) : 1-274
Published By: Brazilian Society of Herpetology
URL: https://doi.org/10.2994/SAJH-D-19-00120.1
Based on a detailed analysis of current seaweed aquaculture practices, regulations, health benefits and consumer demands, these guidelines aim to foster sustainability and protection of the marine environment. These guidelines also include expert opinions and assessmentsfrom the academic, private
and associative sectors, based mainly in Europe, but also on other continents. With this wide scope and using a field-based and scientific approach, we have aimed to produce a robust prospective reference document to support policy-makers and the elaboration of future European regulations.
Rapid Impact Assessment of Climatic and Physio-graphic Changes on Flagship G...Arvinder Singh
‘NATIONAL CONFERENCE ON MAN AND ENVIRONMENT’October 15 – 16, 2012
Organized by
Department of Zoology and Environmental Sciences, Punjabi University, Patiala (Pb.) – 147 002, India
Cyanobacteria, microcystins and cylindrospermopsin in public drinking supply ...M Carmo Oliveira
O Brasil tem um histórico de contaminações em reservatórios de águas destinadas ao abastecimento público por toxinas de cianobactérias. Essas toxinas, entre elas microcistinas e cilindropspermopsinas, causam sérios problemas ao homem. O estudo relata a ocorrência de MC e CYN em 10 reservatórios de abastecimento público do nordeste do Brasil. Devido à similaridade das comunidades de cianobactérias encontradas nos reservatórios e a presença de espécies potencialmente produtoras de toxinas, associada às MC e CYN, o estudo sugere que uma expansão geográfica possa ser favorecida por esses fatores.
Determination of acute toxicity and the effects of sub-acute concentrations o...Nanomedicine Journal (NMJ)
Abstract
Objective(s):
Copper oxidenanoparticles have different industrial applications so it is inevitable that nanoparticulate products finally find their way into aquatic ecosystems. Nevertheless there is little information available about their effects on some of edible fish. The present study aims to determine the acute toxicity and evaluate the effect of two sub-acute concentrations (50 and 70% 96 h LC50) of CuO-NPs on some hematological and biochemical parameters of R. rutilus.
Materials and Methods:
225 healthy specimen of R. rutilus (mean weight 5.52±1.2 g; mean length 6.20±0.2 cm) were transported to the laboratory. In order to prepare the stock solution, CuO-NPs was dispersed in pure water with ultrasonication (50-60 kHz) for 15 min every day before dosing. At first, R. rutilus was exposed to CuO-NPs to determine the lethal concentration (LC50) value. Following acute test, fish were treated with sub-acute concentrations of CuO-NPs (50 and 70% 96 h-LC50 at) with one control group (no CuO-NPs) for a week to determine the changes in the level of some plasma hematological and biochemical parameters.
Results:
The 96 h-LC50 values of CuO-NPs was 2.19±0.003 mg/l. R. rutilus exhibited significantly lower RBC count, Hb and Hct values and a significant increase in the WBC numbers, MCH, MCHC and MCV indices (p<0.05).><0.05).
Conclusion:
These alterations indicate R. rutilus sensitivity to CuO-NPs and changes in blood parameters would be a useful tool for measurement early exposure to CuO nanoparticles.
Evolution & Fisheries: A Relationship?: Dr. Malin Pinskycoseenow
Dr. Malin Pinsky, Rutgers University, presented on his work with respect to the impacts of overfishing on genetic diversity in fishes at the January 9, 2014 STEM Educators' Series.
Metagenomics to Unlock the Biotechnological Potential of Marine Environments by Michele de Cássia Pereira e Silva in Examines in Marine C Biology & Oceanography
Atlas of Brazilian Snakes: Verified Point-Locality Maps to
Mitigate the Wallacean Shortfall in a Megadiverse Snake
Fauna
Authors: Nogueira, Cristiano C., Argôlo, Antonio J.S., Arzamendia,
Vanesa, Azevedo, Josué A., Barbo, Fausto E., et. al.
Source: South American Journal of Herpetology, 14(sp1) : 1-274
Published By: Brazilian Society of Herpetology
URL: https://doi.org/10.2994/SAJH-D-19-00120.1
Based on a detailed analysis of current seaweed aquaculture practices, regulations, health benefits and consumer demands, these guidelines aim to foster sustainability and protection of the marine environment. These guidelines also include expert opinions and assessmentsfrom the academic, private
and associative sectors, based mainly in Europe, but also on other continents. With this wide scope and using a field-based and scientific approach, we have aimed to produce a robust prospective reference document to support policy-makers and the elaboration of future European regulations.
Rapid Impact Assessment of Climatic and Physio-graphic Changes on Flagship G...Arvinder Singh
‘NATIONAL CONFERENCE ON MAN AND ENVIRONMENT’October 15 – 16, 2012
Organized by
Department of Zoology and Environmental Sciences, Punjabi University, Patiala (Pb.) – 147 002, India
El Profesor Erik Simões, tutor del Área de Medio Ambiente de FUNIBER, ha publicado un trabajo sobre las “Mareas Rojas” en el periódico Journal of the Marine Biological Association of the United Kingdom.
Abstract— Coccidiosis swine causes high economic loss, and its prophylaxis is usually performed by the use of chemical drugs. However, these chemical drugs are not allowed in agroecological, organic or biological dynamic systems of production. Additionally, there are concerns about pharmacological resistance and contamination by the presence of chemical residues in the environment and at the food of animal origin. The objective of this study was to evaluate the weight gain and the prevalence of coccidia in piglets submitted to the following treatments: alcoholic extract of propolis 30% (AEP), chemical treatment toltrazuril (CTT), negative control with grain alcohol (NCA) and negative control without treatment (NCT). By means of the individual weights and the number of coccidia at the faeces, it was monitored 216 piglets from commercial farms. Under the conditions of this study none therapeutic intervention provided benefits for weight gain and prevalence of coccidia. Thus, by making it possible the minimization of chemical drug use, these results allow us to suggest the laboratory periodic monitoring as a prophylactic control method for swine coccidiosis.
Antibacterial Activity of Water Extract From Some Folk Medicine at Bidoup-Nuiba National Park, Lam Dong, Vietnam
http://dx.doi.org/10.21276/SSR-IIJLS.2020.6.1.1
Inter Simple Sequence Repeats (ISSR) markers were utilized to identify the levels of heritable varieties and patterns of the populace structure among the five populaces of Pteris biaurita, a natural fern in India. A comprehensive examination was directed in three replicates at 2013-14 seasons in the Western Ghats, South India. Five wild P. biaurita, accessions (maiden hair) were assessed for genotyping studies. Results demonstrated a pivotal discrepancy among genotypes for they were characterized in view of this uniqueness in four groups by the genetic cluster examination. In this trial, ISSR primers amplified 63 polymorphic groups. In view of the genetic identity data, genotypes were figured and differed from 0.5714 to 0.6984. The percentage of polymorphism indicated predominant genotype that may be utilized for the conservation of species. ISSR appeared to be an obliging marker for prediction of genotype inside a closed group of inter specific populace in the investigation territory.
Inter Simple Sequence Repeats (ISSR) markers were utilized to identify the levels of heritable varieties and patterns of the populace structure among the five populaces of Pteris biaurita, a natural fern in India. A comprehensive examination was directed in three replicates at 2013-14 seasons in the Western Ghats, South India. Five wild P. biaurita, accessions (maiden hair) were assessed for genotyping studies. Results demonstrated a pivotal discrepancy among genotypes for they were characterized in view of this uniqueness in four groups by the genetic cluster examination. In this trial, ISSR primers amplified 63 polymorphic groups. In view of the genetic identity data, genotypes were figured and differed from 0.5714 to 0.6984. The percentage of polymorphism indicated predominant genotype that may be utilized for the conservation of species. ISSR appeared to be an obliging marker for prediction of genotype inside a closed group of inter specific populace in the investigation territory