1) The study evaluated the antibacterial and antifungal activity of extracts from different parts of the mangrove plant Avicennia marina against various microbes.
2) The chloroform extract of A. marina roots showed the strongest inhibitory effects against Staphylococcus aureus and Escherichia coli, while the ethanolic extract of roots exhibited antibacterial activity against four bacterial strains tested.
3) The ethyl acetate extract of A. marina leaves showed antibacterial activity against S. aureus and E. coli.
4) The ethanolic extract of A. marina roots and seeds, as well as the ethanolic extract of fruits, demonstrated antifungal activity against As
Multidrug resistant microorganisms are globally becoming a major confrontment because of illogical use of antibiotics and this played a good role in investigation about the antibacterial compounds in plants. Thus, the present study investigate for the antibacterial effect of alcoholic extracts of Curcuma longa L. rhizomes , CommiphoramyrrhaL. gums and Ginkgo biloba L. leaves products against Staphylococcus aureus and Pseudomonas aeruginosa. The plants samples extracted by soxhlet with methanol and fractionation with and four solution ( chloroform, hexane, water and ethyl acetate) were used for investigation about antibacterial activity by disc diffusion method. The results showed that methanolic alcohol extract and fractions of C. longa L. rhizomes , C. myrrha L. gums showed biological activity against P. aeruginosa and S. aureus bacteria, but methanolic alcohol extract and fractions of G. biloba L. leaves product didn’t show any activity as antibacterial substance. It can be concluded that the presence of secondary metabolites as flavonoids, alkaloids, tannins, glycosides and saponins in the plants under study would be marked a good anti-bacterial effect.
Indian medicinal plants have a traditional
background that they have potential to use as antimicrobial agents.
Pedalium murex showed broad spectrum antimicrobial activity
against three fungal strains Aspergillus niger, Aspergillus flavus,
Candida albicans and five bacterial strains Escherichia coli,
Staphylococus epidermis, Klebseilla pneumonia,Citrobactor
diverses, Enterococus faecalis.The Ethanolic extracts were tested
against selected test bacteria and fungi through disc diffusion assay
where amoxicillin was used as standard. The results showed that
alcoholic extract possess good antimicrobial activity against selected
test bacteria and fungi. The present results therefore offer a
scientific basis for traditional use of the various extract of Pedalium
murex
Antibacterial Activity of Stem Bark Extracts of Oroxylum indicum an Endangere...IOSR Journals
The present work has been under taken to study the antibacterial activity of stem bark extracts of O.
indicum against disease causing gram negative and gram positive bacteria. Antimicrobial activity of solvent
extracts of stem bark of Oroxylum indicum has been studied to find out its activity against four important
bacterial strains Bacillus subtilis, B. cereus, Staphylococcus albus and S. aureus . The antimicrobial activity of
the stem bark extracts was done through well diffusion method and by measuring the inhibition zone around the
disc. The results revealed that the aqueous extracts of O. indicum exhibited antimicrobial activity against all the
microbes under study. The results provided evidence that the species O. indicum can be used as a potential
source of antimicrobial agent.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Antibacterial Activity of Leaf Methanolic Extract of S. Caryophyllatum (L.) A...iosrjce
S.caryophyllatum (L.) Alston belongs to the family Myrtaceae is an endangered species. It possesses
traditional as well as pharmacological properties. The objective of the present investigation was to find out the
antibacterial activity of S. caryophyllatum leaf methanolic extract against some human pathogenic bacteria. It
was followed by Disc Diffusion method using gram positive and gram negative bacterial strains such as
Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Bacillus megaterium, Sarcina lutea, Esherichia coli,
Pseudomonas aeruginosa, Klebsiella Spp., Salmonella typhi and Proteus mirabilis. The result showed that the
inhibitory effect on Bacillus subtilis (24mm) was high when compared to E. coli (21mm) and Bacillus cereus
(20mm).This effect on the bacterial strains may be due to the presence of secondary metabolites present in the
leaf methanolic extract of Syzygium caryophyllatum.
Multidrug resistant microorganisms are globally becoming a major confrontment because of illogical use of antibiotics and this played a good role in investigation about the antibacterial compounds in plants. Thus, the present study investigate for the antibacterial effect of alcoholic extracts of Curcuma longa L. rhizomes , CommiphoramyrrhaL. gums and Ginkgo biloba L. leaves products against Staphylococcus aureus and Pseudomonas aeruginosa. The plants samples extracted by soxhlet with methanol and fractionation with and four solution ( chloroform, hexane, water and ethyl acetate) were used for investigation about antibacterial activity by disc diffusion method. The results showed that methanolic alcohol extract and fractions of C. longa L. rhizomes , C. myrrha L. gums showed biological activity against P. aeruginosa and S. aureus bacteria, but methanolic alcohol extract and fractions of G. biloba L. leaves product didn’t show any activity as antibacterial substance. It can be concluded that the presence of secondary metabolites as flavonoids, alkaloids, tannins, glycosides and saponins in the plants under study would be marked a good anti-bacterial effect.
Indian medicinal plants have a traditional
background that they have potential to use as antimicrobial agents.
Pedalium murex showed broad spectrum antimicrobial activity
against three fungal strains Aspergillus niger, Aspergillus flavus,
Candida albicans and five bacterial strains Escherichia coli,
Staphylococus epidermis, Klebseilla pneumonia,Citrobactor
diverses, Enterococus faecalis.The Ethanolic extracts were tested
against selected test bacteria and fungi through disc diffusion assay
where amoxicillin was used as standard. The results showed that
alcoholic extract possess good antimicrobial activity against selected
test bacteria and fungi. The present results therefore offer a
scientific basis for traditional use of the various extract of Pedalium
murex
Antibacterial Activity of Stem Bark Extracts of Oroxylum indicum an Endangere...IOSR Journals
The present work has been under taken to study the antibacterial activity of stem bark extracts of O.
indicum against disease causing gram negative and gram positive bacteria. Antimicrobial activity of solvent
extracts of stem bark of Oroxylum indicum has been studied to find out its activity against four important
bacterial strains Bacillus subtilis, B. cereus, Staphylococcus albus and S. aureus . The antimicrobial activity of
the stem bark extracts was done through well diffusion method and by measuring the inhibition zone around the
disc. The results revealed that the aqueous extracts of O. indicum exhibited antimicrobial activity against all the
microbes under study. The results provided evidence that the species O. indicum can be used as a potential
source of antimicrobial agent.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
Antibacterial Activity of Leaf Methanolic Extract of S. Caryophyllatum (L.) A...iosrjce
S.caryophyllatum (L.) Alston belongs to the family Myrtaceae is an endangered species. It possesses
traditional as well as pharmacological properties. The objective of the present investigation was to find out the
antibacterial activity of S. caryophyllatum leaf methanolic extract against some human pathogenic bacteria. It
was followed by Disc Diffusion method using gram positive and gram negative bacterial strains such as
Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Bacillus megaterium, Sarcina lutea, Esherichia coli,
Pseudomonas aeruginosa, Klebsiella Spp., Salmonella typhi and Proteus mirabilis. The result showed that the
inhibitory effect on Bacillus subtilis (24mm) was high when compared to E. coli (21mm) and Bacillus cereus
(20mm).This effect on the bacterial strains may be due to the presence of secondary metabolites present in the
leaf methanolic extract of Syzygium caryophyllatum.
Biological Efficacy of Quassia Indica (Geratn) Nooteb and Centella Asiatica (...IOSRJPBS
Antimicrobial activity of different extracts of two selected plants, Quassia indica (Geartn) Nooteb and Centella asiatica (L.) Urban were tested against selected strains of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas sps, Bacillus thuringiensis, Klebsiella sps and Serratia sps using agar disc diffusion technique. Petroleum ether, acetone, methanol and water extracts of seed and leaf of Q. indica and leaf of C. asiatica showed moderate to significant antimicrobial activity. Of the four extracts tested, petroleum ether and water fractions showed more activity against all the bacterial strains. The zone of inhibition of the active fractions for the petroleum ether ranged from 8.57 to 12.63 mm and 8.53 to 14.5mm for the water fraction of Q. indica seed and leaf and C. asiatica leaf; and exhibited comparable results with widely used commercial antimicrobial agents (16 to19 mm for Kanamycin and 14 to 17mm for Cefotaxime). The results prove the efficacy of the most active fractions of the selected plant extracts to be used for developing potent antimicrobial formulations.
Comparative analysis of antibacterial activity of Nigella sativa L and Rumex ...theijes
The present study presents the effectiveness of Nigella sativa and Rumex dentatus against some selected microorganisms which are known to cause diseases in human beings and the comparative study of extent of antimicrobial properties of various extracts. Petroleum ether, Methanol and Aqueous extracts of seeds and whole plant respectively were prepared and then tested against pathogenic microorganisms such as Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella pneumonia and Escherichia coli using agar well diffusion method. The zone of inhibition for Nigella sativa was found maximum in petroleum ether extract of 30 mm at a dose of 250μg/ml against Salmonella typhimurium while as at the same concentration the highest inhibition zone diameter of 21mm was recorded against K. pneumoniae for methanol extract in case of Rumex dentatus.
Suillus species, in general, are edible mushrooms, and environmentally important that are associated
mostly with pine trees in the tropics regions. These fungi considered a remarkable source of phenolic
compounds that play a crucial role as antioxidants which may reduce the risk of most human chronic
diseases such as cancer, diabetes, asthma, atherosclerosis, Alzheimer, and others. On the other hand,
carotenoids (β carotene) are the most popular natural pigments which play an important role to protect
the plants from photo-oxidative reactions. In human, these compounds prevent oxidative stress and
expects to have antimicrobial activity. Here, the phenolic compounds were extracted with Ethyl acetate
from fruiting bodies of Suillus sp and analyzed by HPLC, the antioxidant activity (reducing power%) of
phenolic compounds was determined at the concentrations of 1, 2.5, and 5 mg/mL. Antimicrobial activity
of β carotene pigment was measured at a concentration of 100 mg/mL against some human pathogenic
bacteria such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Staphylococcus
aureus. The specific DNA region ITS was amplified and sequenced using ITS1 and ITS4 primers with
some bioinformatics analyses. The phenolic extract isolated from fruiting bodies of Suillus sp showed a
remarkable antioxidant activity by increasing the reducing power percent (from F+3 ions to F+2 ions)
comparing with the industrial antioxidant (Propyl gallate) at all used concentrations. Percent of reducing
power of phenolic compounds were 75.5, 84.9 and 95.7% at concentrations of 1, 2.5, and 5 mg/mL
respectively; comparing with PG were 65.9, 81.3, and 93.3 at 1, 2.5, and 5 mg/mL respectively. The β
carotene pigment revealed a significant antimicrobial activity at a concentration of 100 mg/mL against K.
pneumonia, E. coli, and S. aureus. The highest bacterial growth inhibition was against K. pneumonia (40
mm), followed by E. coli (36 mm) and S. aureus (31 mm), while no effect showed against P. aeruginosa.
Our outcomes revealed that the phenolic bioactive compounds can be used as a natural antioxidant
instead of the industrial antioxidants, and also a β carotene pigment could be applied as a promising
natural compound rather than using the antibiotics and other manufactured compounds to inhibit
bacteria activity.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
A Review on the Antimicrobial Activity of Sesuvium Portulacastrumijtsrd
Mangroves are unique group of vascular plants that occur in saline coastal habitats and are known to tolerate extreme environmental conditions. Some mangrove plants are used for a wide range of conditions, including bacterial, fungal, and viral diseases. The rise of antibiotic-resistant microorganisms is one of the severe problems in healthcare systems of the world, and infectious diseases are the second most serious cause of death worldwide. Therefore, new drugs have to be found in order to combat such diseases and it is essential to find new compounds that have antimicrobial properties. Medicinal-plant extracts, known to produce certain bioactive molecules which react with other organisms in the environment, are known to be less toxic to humans and are environmentally friendly due to the less pollutant released during production. Antimicrobial properties of medicinal plants are being increasingly reported worldwide. Mangroves are biochemically unique and produce a wide array of novel natural products and are considered a rich source of steroids, triterpenes, saponins, flavonoids, alkaloids, and tannins. Extracts from the leaves, stems, barks, and roots of mangrove species have shown positive results for antioxidant activity tests. G. Beulah | D. Divya"A Review on the Antimicrobial Activity of Sesuvium Portulacastrum" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd14540.pdf http://www.ijtsrd.com/biological-science/zoology/14540/a-review-on-the-antimicrobial-activity-of-sesuvium-portulacastrum/g-beulah
Plants are the oldest remedies to cure ailments of mankind. They are a storehouse of bioactive compounds which serve as a lead for the development of therapeutics against many diseases including skin diseases. In the present study, leaf extracts of Aegle marmelos, Nerium indicum, Ricinus communis, and Ziziphus nummularia were selected and tested against common skin pathogens, namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The methanolic extracts of all the four plants were subjected to an assay for antibacterial activity and minimum inhibitory concentration. As antioxidants play a significant role in skin disease treatments, all the extracts were also evaluated for their antioxidant activity. Preliminary phytochemical screening and estimation of total phenolic content were carried out to establish its correlation with All the methanolic extracts showed good activity against the selected skin pathogens with significant minimum inhibitory concentration (MIC) values. N. indicum and A. marmelos showed the highest zone of inhibition against all tested organisms. The extracts possessed potential antioxidant activity against reactive oxygen species with N. indicum exhibiting most potent activity. Further, preliminary phytochemical screening indicated presence high amount of alkaloids, terpenoids, flavonoids, tannins, and saponins in A. marmelos and N. indicum. R. communis and Z. nummularia had the highest amount of phenolic content. The results of the study indicate that traditional knowledge can serve as a guideline to provide leads for further testing of potentially interesting plants to be used as modern treatment alternatives.
Synergistic antibacterial effects of three edible plants extract against anti...Open Access Research Paper
In vitro synergistic antibacterial effects among Alocasia macrorrhizos rhizome, Amorphophallus paeoniifolius corm and Colocasia esculenta corm extracts were tested against six resistant bacteria viz., Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile and Staphylococcus aureus. The inhibition zone was compared with the commercially available antibiotic (tetracycline). High inhibitory activity was observed against E. coli (12.67±0.33 mm) and S. aureus (12.50±0.29 mm) for methanol extract at 800 mgml-1 of concentration. MIC and MBC of the extracts ranged from 200-580 mgml-1 and 250-650 mgml-1 respectively. The lowest MIC and MBC of the extracts were measured against E. coli.
Phytochemical Screening and In-vitro Antibacterial Activity of Mangifera indi...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Invitro Evaluation of Antimicrobial Activity of the plant extracts of Elytrar...IJERA Editor
Antimicrobial activity of the aerial parts of the Elytraria acaulis a stem less perennial herb of Acantheceae family has been carried out in the present study. Extracts of the aerial parts of the plant (Stem & Leaves) were prepared in different organic solvents such as n-Hexane, Ethanol, Methanol and Ethyl acetate. All the extracts were analyzed for antimicrobial properties against various pathogenic bacterial infections (Escherichia coli, Klebsiella Species, and Salmonella Typhi). Amongst all the extracts, methanolic extract exhibited significant antimicrobial activity. The crude methanol extracts of leaves showed good inhibitory effects against pathogens. Hence further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications.
In vitro assessment of antibacterial activity of Salicornia herbacea L. seed ...Innspub Net
In this study, the antibacterial activities of Salicornia herbacea L. seed extract against two gram-negative and two gram- positive bacteria were evaluated with the agar disc diffusion and MIC methods. Result showed that inhibition zones of 9.5±0.01, 6.2±0.00, 4±0.00 and 3.5±0.10 mm for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli, respectively. Among four bacteria the maximum and minimum inhibition seed ethanolic extract were related to S. aureus with inhibition zones of 9.5mm and MIC 189.5 mg/ml and E. coli with inhibition zones of 3.5 mm and MIC 420 mg/ml, respectively. The antimicrobial activity of ethanol seed extract of S. herbacea is the result of phenolic compounds, fatty acids, osmotic compound (betaine)
or synergic and additive effect of several compounds present in it. Our results suggest the possibility of using S. herbacea seed, which possesses strong antibacterial activity, in the treatment of diseases caused by the microorganisms tested. Get the full articles at: http://www.innspub.net/volume-4-number-6-march-2014/
In Vitro Anti-MRSA (Methicillin-Resistant Staphylococcus Aureus) Activities o...iosrjce
The aim of this study was to determine the in vitro anti-MRSA activities of the partitions and
fractions of the crude aqueous leaf extract of Chromolaena odorata against clinical isolates of MRSA.
Partitioning of the extract with chloroform gave two partitions. The aqueous partition (AP) gave a higher yield
of 59.74% than the chloroform partition (CP) with 2.69%. However, the CP showed a higher anti-MRSA
activity. The mean values of zones of inhibition at concentrations of 3.13-25.0mg/ml for the AP and CP were
9.43±1.33mm and 16.61±0.93mm respectively. The minimum inhibitory concentration was 12.5mg/ml for AP
and 3.13mg/ml for CP. Column chromatography of CP resulted to fractions with variety of colours. Thin layer
chromatography of these fractions gave ten fractions with the highest retention factor (Rf) value of 0.93 for
fraction F10 and the least Rf value of 0.43 for fraction F8. At a concentration of 2.0mg/ml, fractions F2 and F3
gave the highest zones of inhibition of 22.5±0.05mm and 22.5±2.50mm respectively while F5 had the least
activity of 8.0±8.0mm. This study suggests that C. odorata is a potential and promising plant that should be
exploited for the management of MRSA diseases
KEY Points of Leicester travel clinic In London doc.docxNX Healthcare
In order to protect visitors' safety and wellbeing, Travel Clinic Leicester offers a wide range of travel-related health treatments, including individualized counseling and vaccines. Our team of medical experts specializes in getting people ready for international travel, with a particular emphasis on vaccines and health consultations to prevent travel-related illnesses. We provide a range of travel-related services, such as health concerns unique to a trip, prevention of malaria, and travel-related medical supplies. Our clinic is dedicated to providing top-notch care, keeping abreast of the most recent recommendations for vaccinations and travel health precautions. The goal of Travel Clinic Leicester is to keep you safe and well-rested no matter what kind of travel you choose—business, pleasure, or adventure.
ICH Guidelines for Pharmacovigilance.pdfNEHA GUPTA
The "ICH Guidelines for Pharmacovigilance" PDF provides a comprehensive overview of the International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH) guidelines related to pharmacovigilance. These guidelines aim to ensure that drugs are safe and effective for patients by monitoring and assessing adverse effects, ensuring proper reporting systems, and improving risk management practices. The document is essential for professionals in the pharmaceutical industry, regulatory authorities, and healthcare providers, offering detailed procedures and standards for pharmacovigilance activities to enhance drug safety and protect public health.
Biological Efficacy of Quassia Indica (Geratn) Nooteb and Centella Asiatica (...IOSRJPBS
Antimicrobial activity of different extracts of two selected plants, Quassia indica (Geartn) Nooteb and Centella asiatica (L.) Urban were tested against selected strains of bacteria viz; Escherichia coli, Staphylococcus aureus, Pseudomonas sps, Bacillus thuringiensis, Klebsiella sps and Serratia sps using agar disc diffusion technique. Petroleum ether, acetone, methanol and water extracts of seed and leaf of Q. indica and leaf of C. asiatica showed moderate to significant antimicrobial activity. Of the four extracts tested, petroleum ether and water fractions showed more activity against all the bacterial strains. The zone of inhibition of the active fractions for the petroleum ether ranged from 8.57 to 12.63 mm and 8.53 to 14.5mm for the water fraction of Q. indica seed and leaf and C. asiatica leaf; and exhibited comparable results with widely used commercial antimicrobial agents (16 to19 mm for Kanamycin and 14 to 17mm for Cefotaxime). The results prove the efficacy of the most active fractions of the selected plant extracts to be used for developing potent antimicrobial formulations.
Comparative analysis of antibacterial activity of Nigella sativa L and Rumex ...theijes
The present study presents the effectiveness of Nigella sativa and Rumex dentatus against some selected microorganisms which are known to cause diseases in human beings and the comparative study of extent of antimicrobial properties of various extracts. Petroleum ether, Methanol and Aqueous extracts of seeds and whole plant respectively were prepared and then tested against pathogenic microorganisms such as Pseudomonas aeruginosa, Salmonella typhimurium, Klebsiella pneumonia and Escherichia coli using agar well diffusion method. The zone of inhibition for Nigella sativa was found maximum in petroleum ether extract of 30 mm at a dose of 250μg/ml against Salmonella typhimurium while as at the same concentration the highest inhibition zone diameter of 21mm was recorded against K. pneumoniae for methanol extract in case of Rumex dentatus.
Suillus species, in general, are edible mushrooms, and environmentally important that are associated
mostly with pine trees in the tropics regions. These fungi considered a remarkable source of phenolic
compounds that play a crucial role as antioxidants which may reduce the risk of most human chronic
diseases such as cancer, diabetes, asthma, atherosclerosis, Alzheimer, and others. On the other hand,
carotenoids (β carotene) are the most popular natural pigments which play an important role to protect
the plants from photo-oxidative reactions. In human, these compounds prevent oxidative stress and
expects to have antimicrobial activity. Here, the phenolic compounds were extracted with Ethyl acetate
from fruiting bodies of Suillus sp and analyzed by HPLC, the antioxidant activity (reducing power%) of
phenolic compounds was determined at the concentrations of 1, 2.5, and 5 mg/mL. Antimicrobial activity
of β carotene pigment was measured at a concentration of 100 mg/mL against some human pathogenic
bacteria such as Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, and Staphylococcus
aureus. The specific DNA region ITS was amplified and sequenced using ITS1 and ITS4 primers with
some bioinformatics analyses. The phenolic extract isolated from fruiting bodies of Suillus sp showed a
remarkable antioxidant activity by increasing the reducing power percent (from F+3 ions to F+2 ions)
comparing with the industrial antioxidant (Propyl gallate) at all used concentrations. Percent of reducing
power of phenolic compounds were 75.5, 84.9 and 95.7% at concentrations of 1, 2.5, and 5 mg/mL
respectively; comparing with PG were 65.9, 81.3, and 93.3 at 1, 2.5, and 5 mg/mL respectively. The β
carotene pigment revealed a significant antimicrobial activity at a concentration of 100 mg/mL against K.
pneumonia, E. coli, and S. aureus. The highest bacterial growth inhibition was against K. pneumonia (40
mm), followed by E. coli (36 mm) and S. aureus (31 mm), while no effect showed against P. aeruginosa.
Our outcomes revealed that the phenolic bioactive compounds can be used as a natural antioxidant
instead of the industrial antioxidants, and also a β carotene pigment could be applied as a promising
natural compound rather than using the antibiotics and other manufactured compounds to inhibit
bacteria activity.
International Journal of Pharmaceutical Science Invention (IJPSI)inventionjournals
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
A Review on the Antimicrobial Activity of Sesuvium Portulacastrumijtsrd
Mangroves are unique group of vascular plants that occur in saline coastal habitats and are known to tolerate extreme environmental conditions. Some mangrove plants are used for a wide range of conditions, including bacterial, fungal, and viral diseases. The rise of antibiotic-resistant microorganisms is one of the severe problems in healthcare systems of the world, and infectious diseases are the second most serious cause of death worldwide. Therefore, new drugs have to be found in order to combat such diseases and it is essential to find new compounds that have antimicrobial properties. Medicinal-plant extracts, known to produce certain bioactive molecules which react with other organisms in the environment, are known to be less toxic to humans and are environmentally friendly due to the less pollutant released during production. Antimicrobial properties of medicinal plants are being increasingly reported worldwide. Mangroves are biochemically unique and produce a wide array of novel natural products and are considered a rich source of steroids, triterpenes, saponins, flavonoids, alkaloids, and tannins. Extracts from the leaves, stems, barks, and roots of mangrove species have shown positive results for antioxidant activity tests. G. Beulah | D. Divya"A Review on the Antimicrobial Activity of Sesuvium Portulacastrum" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-4 , June 2018, URL: http://www.ijtsrd.com/papers/ijtsrd14540.pdf http://www.ijtsrd.com/biological-science/zoology/14540/a-review-on-the-antimicrobial-activity-of-sesuvium-portulacastrum/g-beulah
Plants are the oldest remedies to cure ailments of mankind. They are a storehouse of bioactive compounds which serve as a lead for the development of therapeutics against many diseases including skin diseases. In the present study, leaf extracts of Aegle marmelos, Nerium indicum, Ricinus communis, and Ziziphus nummularia were selected and tested against common skin pathogens, namely Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The methanolic extracts of all the four plants were subjected to an assay for antibacterial activity and minimum inhibitory concentration. As antioxidants play a significant role in skin disease treatments, all the extracts were also evaluated for their antioxidant activity. Preliminary phytochemical screening and estimation of total phenolic content were carried out to establish its correlation with All the methanolic extracts showed good activity against the selected skin pathogens with significant minimum inhibitory concentration (MIC) values. N. indicum and A. marmelos showed the highest zone of inhibition against all tested organisms. The extracts possessed potential antioxidant activity against reactive oxygen species with N. indicum exhibiting most potent activity. Further, preliminary phytochemical screening indicated presence high amount of alkaloids, terpenoids, flavonoids, tannins, and saponins in A. marmelos and N. indicum. R. communis and Z. nummularia had the highest amount of phenolic content. The results of the study indicate that traditional knowledge can serve as a guideline to provide leads for further testing of potentially interesting plants to be used as modern treatment alternatives.
Synergistic antibacterial effects of three edible plants extract against anti...Open Access Research Paper
In vitro synergistic antibacterial effects among Alocasia macrorrhizos rhizome, Amorphophallus paeoniifolius corm and Colocasia esculenta corm extracts were tested against six resistant bacteria viz., Yersinia enterocolitica, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Clostridium difficile and Staphylococcus aureus. The inhibition zone was compared with the commercially available antibiotic (tetracycline). High inhibitory activity was observed against E. coli (12.67±0.33 mm) and S. aureus (12.50±0.29 mm) for methanol extract at 800 mgml-1 of concentration. MIC and MBC of the extracts ranged from 200-580 mgml-1 and 250-650 mgml-1 respectively. The lowest MIC and MBC of the extracts were measured against E. coli.
Phytochemical Screening and In-vitro Antibacterial Activity of Mangifera indi...iosrphr_editor
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
Invitro Evaluation of Antimicrobial Activity of the plant extracts of Elytrar...IJERA Editor
Antimicrobial activity of the aerial parts of the Elytraria acaulis a stem less perennial herb of Acantheceae family has been carried out in the present study. Extracts of the aerial parts of the plant (Stem & Leaves) were prepared in different organic solvents such as n-Hexane, Ethanol, Methanol and Ethyl acetate. All the extracts were analyzed for antimicrobial properties against various pathogenic bacterial infections (Escherichia coli, Klebsiella Species, and Salmonella Typhi). Amongst all the extracts, methanolic extract exhibited significant antimicrobial activity. The crude methanol extracts of leaves showed good inhibitory effects against pathogens. Hence further studies on this plant will enable elucidation of its therapeutic properties and medicinal applications.
In vitro assessment of antibacterial activity of Salicornia herbacea L. seed ...Innspub Net
In this study, the antibacterial activities of Salicornia herbacea L. seed extract against two gram-negative and two gram- positive bacteria were evaluated with the agar disc diffusion and MIC methods. Result showed that inhibition zones of 9.5±0.01, 6.2±0.00, 4±0.00 and 3.5±0.10 mm for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli, respectively. Among four bacteria the maximum and minimum inhibition seed ethanolic extract were related to S. aureus with inhibition zones of 9.5mm and MIC 189.5 mg/ml and E. coli with inhibition zones of 3.5 mm and MIC 420 mg/ml, respectively. The antimicrobial activity of ethanol seed extract of S. herbacea is the result of phenolic compounds, fatty acids, osmotic compound (betaine)
or synergic and additive effect of several compounds present in it. Our results suggest the possibility of using S. herbacea seed, which possesses strong antibacterial activity, in the treatment of diseases caused by the microorganisms tested. Get the full articles at: http://www.innspub.net/volume-4-number-6-march-2014/
In Vitro Anti-MRSA (Methicillin-Resistant Staphylococcus Aureus) Activities o...iosrjce
The aim of this study was to determine the in vitro anti-MRSA activities of the partitions and
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2. Plants 2021, 10, 252 2 of 14
available for these fungi because of increased resistance mechanisms through the biofilms’
formation by the fungal strains [2].
It is well known that plant parts as a whole or their extracts in different solvents are
applied for various health ailments since time immemorial. Since natural herbal remedies
of diseases are the outcome of hundreds of years of careful evaluation of their therapeutic
efficacy and risks, side effects, and properties of these herbal based treatments are well
known. Further, the present consumers’ interest is for such natural foods that should be
effective antimicrobial agents with no side effects [4]. Mangroves are a group of trees and
shrubs that live in the coastal intertidal zone. Mangrove trees can grow under adverse
environmental conditions like low-oxygen soil, high temperature, high salinity, etc. The
distribution of mangrove forests is mainly at the tropical and subtropical latitudes [5].
Recently, many studies have directed towards investigating the biological activity of
mangroves for the treatment of various diseases. Recently, Ser et al. [6] isolated two strains
of Streptomyces from Peninsular Malaysia mangrove sediments. Bioactivity screening of
these strains showed potent antioxidant and cytotoxic activities against human cancer
cell lines [6]. Several bioactive compounds have been isolated and characterized from
mangrove plants. These belong to the categories of glycosides, tannins, terpenes, steroids,
naphthoquinones, alkaloids, and flavonoids [7]. Due to the presence of the varied levels of
bioactive compounds, research interest in mangrove plants for their therapeutic activities,
including antimicrobial effects, is increasing continuously [8].
Avicennia marina (Forssk.) Vierh., a mangrove tree belonging to the Acanthaceae family,
is mostly found in the subtropical and tropical regions of the Indo-West-Pacific area. It is
considered a representative example of mangroves that have been widely investigated for
their medicinal importance [9]. This tree withstands severe environmental stresses [10,11].
It has been reported that A. marina can tolerate a very high level of salinity and tempera-
ture [12]. The survival capability of A. marina under severe environmental conditions is
because of the presence of a large number of phytochemicals [13]. The presence of the vari-
ous categories of phytochemicals makes this plant an excellent candidate for the treatment
of various health ailments. Avicennia marina is used in the traditional medicine system for
many centuries. For example, leaves are used for the treatment of ulcers, abscesses, and
rheumatism. The leaf decoction is also used for the treatment of malarial fever and food
poisoning [14]. Ringworms, skin ulcers, and scabies are treated using barks and fruits [15].
ElDohaji et al. [16] systematically reviewed the medicinal properties of A. marina and
reported multiple health benefits. Various researchers are conducting studies on evaluating
the medicinal properties of A. marina through in vitro experimentations. Evaluation of the
antiviral activity of methanolic, ethanolic, aqueous, and n-hexane extracts of A. marina
against HIV-1 and HSV have shown that the maximum antiviral activity was with the
application of methanol extract [17]. Behbahani et al. [18] isolated luteolin-7-O-methyl
ether-3’-O-β-d-glucoside from A. marina, and they showed antiherpetic activity of AM to
this compound. It was speculated that this compound probably hinders the attachment of
HSV to the cell membrane, and, thereby, HSV entry into the cell. Additional studies on
the evaluation of the biological activity of the methanolic extract of A. marina seeds and its
column chromatography-based fractions have reported an iridoid glycoside that restricts
the replication of HIV-1 in the initial stage of infection [19]. Besides the antiviral activity,
the antibacterial activity of AM extracts was also investigated [20,21]. However, a detailed
investigation of antibacterial and antifungal activity of the extracts of different parts of
A. marina in different solvent systems has not been evaluated. Given this, the present study
has shown in vitro antibacterial and antifungal activity of fractions of ethanolic extracts of
different parts of A. marina in various solvents (water, ethanol, ethyl acetate, petroleum
ether, and chloroform) against Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus,
and Escherichia coli bacterial strains and Aspergillus fumigatus, Candida albicans, and Mucor sp.
fungal strains.
3. Plants 2021, 10, 252 3 of 14
2. Results
Preliminary screening of the extracts of various parts of AM in different solvents for
antimicrobial activity revealed that only root and leaves exhibited antibacterial activity.
Antifungal activity was shown by the root, fruit, and seeds. These parts of the AM were
taken for further study.
2.1. Antibacterial Activity of Extract of the Roots of Avicennia marina
Minimal inhibitory concentration (MIC) values were used to determine the suscepti-
bilities of bacteria to drugs and also to evaluate the activity of new antimicrobial agents. As
illustrated in Figure 1, using an agar diffusion method with commercially available strips
containing an exponential gradient of antibiotic, the chloroform extract of AM roots exhib-
ited the lowest MIC for S. aureus and E. coli with concentrations of 1.5 ± 0.03 mg/mL and
1.7 ± 0.01 mg/mL, respectively that was lower than the control values (3.0 ± 0.2 mg/mL
and 3.5 ± 0.34 mg/mL, respectively). Antibacterial activity was also observed by the
application of the ethanolic extract of AM roots. The MIC values for P. aeruginosa, B. subtilis,
S. aureus, and E. coli were 10.8 ± 0.78, 6.1 ± 0.27, 2.3 ± 0.08, and 6.3 ± 0.28 mg/mL, respec-
tively with the application of ethanolic extract when compared with the control values of
4.6 ± 0.22, 5.1 ± 0.25, 3.0 ± 0.2, and 3.5 ± 0.34, respectively. No significant effects of ethyl
acetate, petroleum ether, and aqueous extracts of AM roots were reported on any of the
tested bacterial strains.
Figure 1. Minimal inhibitory concentration (MIC) values for ethanol and chloroform Avicennia marina
roots extract against P. aeruginosa, B. subtilis, S. aureus, and E. coli. Tetracycline hydrochloride was a
positive control. Aqueous, petroleum ether, and ethyl acetate extracts did not affect any of the tested
bacterial strains. Chloroform extract did not affect B. subtilis. Values are the mean of five replicates
(n = 5). Vertical bars show standard error.
2.2. Antibacterial Activity of the Leaf Extract of Avicennia marina
Among the different solvent extracts of the leaves of AM, significant antibacterial
activity was observed by applying the extract of leaves with ethyl acetate. The MIC values
for the ethyl acetate extract of AM leaves against S. aureus, and E. coli were 1.2 ± 0.1 mg/mL
and 1.4 ± 0.15 mg/mL, compared to the MIC values of 3.0 ± 0.21 and 3.5 ± 0.29 mg/mL,
respectively of the control. On the other hand, all ethanol, petroleum ether, chloroform,
and aqueous extracts had no significant effects on any tested bacterial strains (Figure 2).
4. Plants 2021, 10, 252 4 of 14
Figure 2. MIC values for ethyl acetate Avicennia marina leaf extract against P. aeruginosa, B. subtilis,
S. aureus, and E. coli. Tetracycline hydrochloride was a positive control. Aqueous, chloroform, ethanol,
and petroleum ether extracts did not affect any of the tested bacterial strains. Ethyl acetate extract
did not affect P. aeruginosa and B. subtilis. Values are the mean of five replicates (n = 5). Vertical bars
show standard error.
2.3. Minimal Bactericidal Concentration for Extract of the Roots of Avicennia marina
The minimum bactericidal concentration (MBC) could be used as a confirmatory
parameter for the antibacterial activity of any drug. As illustrated in Figure 3, the MBC
was reported by applying the chloroform extract of the root of the AM. The values of MBC
for the chloroform extract of AM roots against S. aureus and E. coli were 3.2 ± 0.09 mg/mL
and 2.9 ± 0.11 mg/mL, respectively in comparison to the control (6.6 ± 0.31 and 6.4 ± 0.39)
mg/mL. No MBC was observed by applying extracts of the roots of AM in ethanol,
petroleum ether, ethyl acetate, and water on the tested bacterial strains.
Figure 3. MBC values for chloroform Avicennia marina roots extract against P. aeruginosa, B. subtilis,
S. aureus, and E. coli. Tetracycline hydrochloride was a positive control. Aqueous, ethanol, petroleum
ether, and ethyl acetate extracts did not affect any tested bacterial strains. Chloroform extract did not
affect P. aeruginosa and B. subtilis. Values are the mean of five replicates (n = 5). Vertical bars show
standard error.
5. Plants 2021, 10, 252 5 of 14
2.4. Minimal Bactericidal Concentration for Extracts of Leaves of Avicennia marina
As illustrated in Figure 4, significant minimal bactericidal concentration (MBC) was
reported with the application of ethyl acetate extract of the leaves of AM. The MBC for
the ethyl acetate extract of AM leaves against S. aureus and E. coli were 14.2 ± 0.5 mg/mL
and 12.5 ± 0.32 mg/mL, as compared to the control MBC values of 6.3 ± 0.21 and
5.1 ± 0.19 mg/mL, respectively. Extract of leaves in ethanol, petroleum ether, chloro-
form, and water did not significantly affect any of the tested bacterial strains.
Figure 4. MBC values for ethyl acetate Avicennia marina leaf extract against P. aeruginosa, B. subtilis,
S. aureus, and E. coli. Tetracycline hydrochloride was a positive control. Aqueous, ethanol, petroleum
ether, and chloroform extracts did not affect any tested bacterial strains. Ethyl acetate extract did not
affect P. aeruginosa and B. subtilis. Values are the mean of five replicates (n = 5). Vertical bars show
standard error.
2.5. Antifungal Activity of Extract of Different Parts of Avicennia marina
Antifungal activity was also examined to evaluate the biological activity of AM on
different types of fungal strains, viz. Aspergillus fumigatus, Candida albicans, and Mucor sp.
Extracts of roots, fruits, and seeds were carried out using ethanol, ethyl acetate, petroleum
ether, chloroform, and water, and antifungal activity of these extracts was monitored on
three different fungal strains (Figure 5). Among the extracts of the AM root in different
solvents, antifungal activity was observed by the application of ethanolic extract on As-
pergillus fumigatus only. Ethanolic extract of the AM root did not show the antifungal effect
on Candida albicans and Mucor sp. The MIC value was lower (0.25 ± 0.01 mg/mL) than
that of the control fluconazole (0.6 ± 0.03 mg/mL). Extracts of the AM root in ethyl acetate,
petroleum ether, chloroform, and water did not show any antifungal effects on any fungal
strain (Figure 5a). Among the extracts of AM fruits in different solvents, the antifungal
effect was reported by applying the ethanolic extract that showed an antifungal effect
against Aspergillus fumigatus and Candida albicans. The MIC values of 0.26 ± 0.02 mg/mL
and 0.25 ± 0.01 mg/mL of Aspergillus fumigatus and Candida albicans, respectively, were
lower than the control values of 0.42 ± 0.2 mg/mL and 0.6 ± 0.31 mg/mL, respectively
(Figure 5b). Among the extracts of the seeds of AM in different solvents, only ethanolic
extract showed antifungal activity. This antifungal activity was limited to the A. fumigatus
with a MIC value of 0.26 ± 0.01 mg/mL lower than the control (0.45 ± 0.02 mg/mL)
(Figure 5c).
6. Plants 2021, 10, 252 6 of 14
Figure 5. MIC values for the ethanolic extract of the root (A), fruits (B), and seeds (C) of Avicennia
marina against Aspergillus fumigatus, Candida albicans, and Mucor sp. Fluconazole was used as the
control. Ethyl acetate, petroleum ether, chloroform, and aqueous extracts of root, fruit, and seeds of
Avicennia marina did not show any inhibitory effects on the tested fungal strains. Only ethanol extract
showed an inhibitory effect on A. fumigatus and C. albicans. Values are the mean of five replicates
(n = 5). Vertical bars show standard error.
7. Plants 2021, 10, 252 7 of 14
2.6. Electron Microscopic Studies
Antifungal activity for the ethanolic extract of AM fruits was further validated through
image analysis of the treated and untreated fungal colonies using the scanning electron
microscope (SEM). Micrographs of the colony of Candida albicans, treated with the ethanolic
extract of the fruits of AM, showed a ruptured, incorporated, and shrinkage fungal hypha
(Figure 6b). Contrary to this, the fungal hypha of the untreated colonies of the Candida
albicans was normal in structure and shape (Figure 6a). Similarly, the colonies of the
Aspergillus fumigatus (Figure 7b), treated with the ethanolic extract of the fruits of the
AM, showed cytolysis and loss of cell integrity, compared to that of the untreated colony
(Figure 7a).
Figure 6. Scan electron microphotographs of untreated (A) and treated (B) Candida albicans.
Figure 7. Scan electron microphotographs of untreated (A) and treated (B) Aspergillus fumigatus.
3. Discussion
Humans have bee using plants for the treatment of various infectious diseases since
ancient times. Scientific research for proving the therapeutic efficacy of a large number of
medicinal plants are undergoing. Today, medicinal plants are being used in many countries
for the treatment of different infectious diseases. The current interest in medicinal plants
as therapeutic agents has emerged in various parts of the world because of the increasing
incidence of drug-resistant bacteria and the appearance of new pathogenic bacteria strains.
8. Plants 2021, 10, 252 8 of 14
In vitro testing of a large number of plants has been carried out against different bacterial
strains, and it has been reported that extracts and pure compounds of many medicinal
plants are very effective against bacterial strains [22]. Many scientific reports have shown
potential foliar extracts of mangrove against microbial pathogens and suggested consid-
ering the mangrove plants as a valuable source for the bioactive chemicals of immense
medicinal values [23–25]. The present study has shown in vitro experimental evaluation
of the effectiveness of antibacterial activity of extracts of plant parts of A. marina (a man-
grove tree) in different solvent systems against Bacillus subtilis, Escherichia coli, Pseudomonas
aeruginosa, and Staphylococcus aureus. The antifungal activity of these extracts was also
evaluated against Aspergillus fumigatus and Candida albicans. This study reported that
the ethanolic extracts of AM roots showed antibacterial effects against all the four tested
bacteria, viz., B. subtilis, E. coli, P. aeruginosa, and S. aureus. Root extract in chloroform
showed antibacterial activity against E. coli, P. aeruginosa, and S. aureus. The root extracts in
other solvents did not show antibacterial activity on the tested bacteria. These observations
are in agreement with earlier reports showing the antibacterial activity of the alcoholic and
the chloroform root extracts of AM [26]. While the extract of leaves of AM in ethyl acetate
exhibited antibacterial activity against S. aureus and E. coli, none of the extracts in ethanol,
petroleum ether, chloroform, and water showed antibacterial activity in our study. Active
components present in the plant extracts are responsible for the differential antibacterial
activity as Avicennia marina accumulated many phytochemicals for its survival under severe
environmental conditions. The phytochemicals belong to phenolics, steroids, triterpenes,
glycosides, esters, aliphatic alcohols, amino acids, carotenoids, etc. [27,28]. The GC–MS
based analysis of the chemical composition of extracts of plants parts in different solvent
systems revealed the presence of some notable compounds like 1,2-Benzenedicarboxylic
acid, Cis-cinnamic acid, hexadecanoic acid, 2,6,10,14,18,22-tetracosahexae, 25-ethyl-27-
norcholesta-5,24(Z), 1-tetradecene, taraxasterol, hydroxymethylfurfural (HMF), and 1-
deoxy-D-altritol (Supplementary Table S1). These compounds have proven antimicrobial
activity. Khattab and Temraz [29] also reported 2-propenoic acid, 3-phenyl ester, 3-acetyl
methoxyphenyl, benzaldehyde, 3-hydroxyl-4-methoxy, and 1,2-benzenediol in the leaf
extract of A. marina and phosphonic acid and p-hydroxyphenyl in the flower extract.
Naphthofuran compounds like naphtho[1,2-b]furan-4,5-dione, 2-[20-20-hydroxypropyl]-
naphtho[1,2-b] furan-4,5-dione, and 3-hydroxynaphtho[1,2-b] furan-4,5-dione were iden-
tified by Sutton et al. [30]. Isoverbascoside and D-rhamnosyl verbascoside were isolated
from the leaves of A. marina [31]. Some important sterols like stigmasterol-3-O-β-D galac-
topyranoside [32], stigmasterol [33], β-sitosterol, and ergost-6,22-diene-5,8-epidioxy-3 β-
ol [34] were also identified from the aerial roots and leaves of this plant. Wu et al. [35]
extensively reviewed the worldwide mangrove species and summarized 349 metabolites
of these plants in terms of source, chemistry, and bioactivity. The presence of identified
chemical compounds in the extracts of plant parts of A. marina in different solvent systems
in our study also matched with the compounds reported earlier in other mangrove plants.
The chemical properties of the solvent used in the extraction process played a crucial role
in exhibiting the plants’ antibacterial properties. The presence of these phytochemicals
in the solvents may be correlated with the differential antibacterial activity against the
bacterial strains. Ethanolic extracts of the roots proved the most effective in almost all
the antibacterial tests, followed by the chloroform, indicating that maximum antibacterial
active ingredients of A. marina roots were extracted into ethanol. It is suggested that the in-
ability of the extracts of plant parts in other solvent systems to exhibit antibacterial activity
against tested bacterial strains maybe because these strains of bacteria might have a kind
of resistance mechanisms, e.g., modification of target sites, inactivation of enzymes, and
reduced accumulation of drug, or the quantity of the bioactive compounds is very low [36].
Since control showed no inhibition, it proves that solvents did not act as antibacterial agents.
Leaf extract of A. marina in ethyl acetate only showed antibacterial activity in our study. The
presence of phytol (a constituent of chlorophyll, which could be converted to phytanic acid)
in the leaves extract could be responsible for the antibacterial effects. Similarly, Raut and
9. Plants 2021, 10, 252 9 of 14
Anthappan [37] have documented antibacterial activity for the methanolic leaves extract
of the mangrove, Sonneratia alba, and the effect was obvious for both S. aureus and E. coli
among the different tested bacterial strains.
Analysis of the antifungal activity of the A. marina in our study indicated that the
extract of the roots, fruits, and seeds of A. marina showed antifungal activity against
Aspergillus fumigatus and Candida albicans. Khafagi et al. [38] has also reported antifungal
activity of both aqueous and ethanol extracts of roots, cotyledons, leaves, and stems
of Avicennia marina. Taken together, our data indicated that mangroves in general and
Avicennia marina, in particular, have potent biological activity against an extensive array of
microorganisms. These effects are primarily dependent on the plant parts and the solvent
used or the extraction process.
The most common mode of the action of different bioactive compounds that are used to
treat microbial infections is the interaction with the microbial enzyme system, interference
with nucleic acids, interference with the cell membrane and cell wall, etc. [39–42]. Benzoic
acid and phthalate, as identified in this study in the ethanolic extract of A. marina, have
been reported to have a permeability barrier provided by the cells membrane, which
is indispensable to many cellular functions, including maintaining the energy status of
the cell, membrane coupled energy transducing process, solute transport, and metabolic
regulation [43,44]. Stigmasterol, another compound of the plant extract of A. marina, has
been reported to act as a β-lactamase inhibitor, which restores susceptibility of the antibiotic
resistant bacteria to antibiotics [45]. Cinnamic acid and its hydroxylated derivatives have
also been reported to have antifungal activity through their ability to prevent fungal spore
germination and antityrosinase enzyme activity [46]. It has also been reported that the
cinnamic acids caused fungal growth inhibition by interacting with benzoate 4-hydroxylase,
an enzyme responsible for aromatic detoxification [47]. Hydroxymethylfurfural (HMF)
has also been reported in the extracts of A. marina. The HMF is a honey component where
it works as an antimicrobial agent, possibly because of its high osmolarity and acidity
(low pH) [48,49]. Johannes et al. [50] reported that hexadecanoic acid reacted with the
hydroxyl group of lipopolysaccharide, a component of the bacterial cell wall, resulting in
the conversion of lipopolysaccharide membrane structure into the asymmetric form. As
a result, the balance in the membrane lipid structure is disturbed, causing perturbance
in the cell membrane. This change in the cell membrane resulted in the cell swelling and
cytoplasm membrane damage, distended, and lysed. OH group of hexadecanoic acid has
been reported to be toxic to the cell protoplasm. It infiltrates and damages the cell wall. It
also causes denaturation of the cytoplasm protein Radiati [51].
4. Materials and Methods
4.1. Plant Material and Preparation of Extracts
Root, stem, leaves, stems, fruits, and seeds of Avicennia marina (Forssk.) Vierh. (Avi-
cenniaceae) were collected from the mangrove stand of Jazan area that is located in the
southern region of Saudi Arabia (Farasan Island, 16◦4202100 N 41◦590000 E). The collected
material was taxonomically identified, and the voucher specimen was kept in the herbar-
ium of the Botany and Microbiology Department, College of Science, King Saud University,
Saudi Arabia (Voucher No.: 23638). These plant parts were separately washed thoroughly
with seawater to remove epiphytes, shells, and various extraneous matters. The cleaned
plant parts were packed separately in polyethylene bags and brought to the laboratory.
The collected samples were air-dried under shade. The dried plant parts were powdered
separately, and homogenous powder of each plant part was obtained. Each plant part
(100 g) was extracted with 95% 1000 mL ethanol with stirring for 72 h. Filtration of the
extract was then carried out using a muslin cloth, centrifuged at 9000 rpm for 10 min.
To obtain clear filtrate, the extract was filtered again using Whatman filter paper No. 41.
The filtrates were evaporated and dried using a vacuum rotary evaporator to obtain the
active ingredients in their solid phase. These solid active ingredients were then dissolved
in ethanol solution (ethanol: water, 4:6, v/v). The sequential separation was performed
10. Plants 2021, 10, 252 10 of 14
with petroleum ether, ethyl acetate, ethanol, chloroform, and water to yield the respective
solvent fractions. These fractions were then concentrated to get dried extract for further
analysis of biological activities.
4.2. Microbial Strains and Culture Media
Four different pathogenic bacterial strains: Bacillus subtilis (ATCC 10400), Escherichia
coli (ATCC 442), Pseudomonas aeruginosa (ATCC 27853), and Staphylococcus aureus (ATCC
29213), were used for this study. Staphylococcus aureus and B. subtilis are Gram-positive
bacteria. Escherichia coli, S. sonnei, and P. aeruginosa are Gram-negative bacteria. These
bacterial strains were procured from the Botany and Microbiology Department of King
Saud University, Saudi Arabia. Fungal strains were Aspergillus fumigatus (ATCC46645),
Candida albicans (ATCC28121), and Mucor sp. (Clinical isolates). These fungal strains were
procured from the military hospital in Riyadh, Saudi Arabia. The bacterial species were
grown in Mueller–Hinton agar (Merck, USA). Fungal species were grown in the Sabouraud
dextrose broth medium.
4.3. Preparation of Inoculums
Each strain of bacteria from the stock cultures was streaked on the agar plate. The
plate was then incubated for 24 h at 37 ◦C. Three bacterial colonies from each plate were
emulsified in sterile 0.9% NaCl (w/v) to obtain 108 CFU per mL (0.5 McFarland scale) as
inoculums. The working bacterial suspension for the disk diffusion test was prepared
through dilution with 0.9% sterile NaCl (w/v) until 107 CFU per mL was reached. Similarly,
fungal suspensions were adjusted to 107 cells/mL.
4.4. Analysis of Antibacterial Activity
Evaluation of the antimicrobial activity of plant extracts was carried out using the disk
diffusion method. Sterile filter paper disks (6 mm in diameter) were prepared and placed
in Petri dishes. Fifty micrograms of residues of plant extract were redissolved separately.
After sterilization, through a 0.22 mm Millipore filter, the plant extracts were loaded over
filter paper discs, and a final concentration of 10 mg/mL was maintained. Mueller–Hilton
agar was poured into Petri dishes. Each Petri dish was loaded with 150 µL of microbial
suspension (1 × 1016 cells/mL) and then with filter paper discs containing plant extracts of
different plant parts. One filter paper disc containing 5 mg of tetracycline hydrochloride
(European Pharmacopoeia Reference Standard, Sigma-Aldrich, St. Louis, MO, USA), and
one filter paper disc containing only the solvent (without plant extract) were also placed
on the top of Mueller–Hilton agar plates in separate Petri dishes for positive control and
negative control, respectively. The solvent for tetracycline hydrochloride was dimethyl
sulfoxide. All the Petri dishes were kept at 5 ◦C for 2 h for the diffusion of plant extract
and then incubated at 37 ◦C for 24 h anaerobically. Incubation results were monitored.
4.5. Determination of Minimum Inhibitory Concentration (MIC)
Minimum inhibitory concentration (MIC) of plant extracts that exhibiting a strong
antimicrobial activity was evaluated against different bacterial strains. Sterilized extracts
of plants parts in various solvents at a final concentration of 0.25, 0.75, 1.5, 2.5, 5.0, 7.5,
10.0, 12.5, and 15.0 mg/mL were loaded over filter paper discs. Mueller–Hilton agar was
poured into Petri dishes. Each Petri dish was loaded with 150 µL of microbial suspension
(1 × 1016 cells/mL), and then with filter paper discs containing plant extracts of different
plant parts in various solvents. One filter paper disc containing 5 mg of tetracycline
hydrochloride and one filter paper discs containing only the solvent (without plant extract)
were also placed on the top of Mueller–Hilton agar plates in separate Petri dishes for
positive control and negative control, respectively. All the Petri dishes were kept at 5 ◦C
for 2 h for the diffusion of plant extract and then incubated at 37 ◦C for 24 h anaerobically.
The MIC was determined as the minimum concentration of the plant extract that inhibits
bacterial growth.
11. Plants 2021, 10, 252 11 of 14
4.6. Determination of Minimum Bactericidal Concentration (MBC)
For the five different bacterial strains used, the MBC of plant extract was determined
following the method described previously [52]. Bacterial streaks of the Petri dishes
in which plants showed an antibacterial effect were taken from the inhibition zone of
MIC plates. These bacterial streaks were placed on TSA (tryptone soya agar) plates and
incubated at 35 ◦C for 24 h. Bacterial growth was examined in each Petri dish. The MBC
was recorded as the concentration of extract of plant parts in different solvents that showed
no bacterial growth.
4.7. Analysis of Antifungal Activity and Minimum Fungicidal Concentration
Aspergillus fumigatus (ATCC46645), Candida albicans (ATCC28121), and Mucor sp. were
grown in Sabouraud dextrose (SD) broth at 27 ◦C for 48 h. The growth of the fungal strains
was adjusted to OD600 of 0.1. Extracts of plant parts were filtered through 0.45 µm Millipore
filters. SD agar was placed on several Petri dishes. Of suspension 100 µL containing 1.0 ×
108 CFU/mL of each fungal strain was spread on several Petri dishes. Filter paper disks
(7 mm) were impregnated with 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, and 1.0 mg/mL extract
of plant parts. One set of the disc was kept as negative controls in which only the solvents
were used without plant extracts. One set of the disc was used as a positive control in
which fluconazole (≥98% pure, Sigma-Aldrich, St. Louis, MO, USA) was added in place of
plant extracts. The solvent for fluconazole was dimethyl sulfoxide. The inoculated Petri
dishes were incubated at 27 ◦C for 72 h. Antifungal activity was monitored by analysis of
the inhibition zone.
Minimum fungicidal concentration (MFC) was determined by adding different con-
centrations of plant extracts (0.1–1 mg/mL) were incorporated in SD broth in tubes. Of
fungal suspension 1 mL was added to each tube. Incubation was carried out at room
temperature for three days. Negative control was made in one tube that contained only
the solvents. One tune was made as a positive control in which fluconazole was added in
place of plant extracts. The lowest concentration of the plant extract inhibiting the growth
of the fungus is regarded as the MFC.
4.8. Scan Electron Microscopic Analysis
For electron microscopic analysis, samples were prepared and investigated under the
scanning electron microscope (JSM-7610F, JEOL, Tokyo, Japan), located in the Research
Center of College of Science, King Saud University, Saudi Arabia.
4.9. Statistical Analysis
Data were subjected to the statistical analysis using the one-way analysis of variance
(ANOVA). Means were separated by the least significant difference test (LSD, p ≤ 0.05)
using the SPSS 21.0 statistical program “SPSS Inc., IBM” (Chicago, IL, USA). All measure-
ments were performed five times for each treatment, and the means were reported.
5. Conclusions
Increasing resistance of the bacteria and fungi towards various antimicrobial syn-
thetic drugs poses a major concern in medical science. It is well established from the
ethnopharmacological investigations that the plants possess biologically active antimicro-
bial compounds. The present study has shown that extracts of root and leaves of A. marina
exhibited antibacterial activity. Ethanolic extract of root proved to be the most effective
against bacterial strains, P. aeruginosa, B. subtilis, S. aureus, and E. coli and fungal strains
Aspergillus fumigatus and Candida albicans. Leaf extract in ethyl acetate showed significant
antibacterial activity against S. aureus and E. coli. The antifungal activity has also been
shown by the ethanolic extracts of fruit and seeds. The extracts of this plant were effective
against S. aureus, known for its increased resistance to antibiotics. Therefore, this plant
can be used to treat various diseases caused by antibiotic-resistant bacteria. There are a
lot of potentials for this mangrove plant to treat infections caused by antibiotic-resistant
12. Plants 2021, 10, 252 12 of 14
bacteria. However, further research is required to understand the mechanisms involved in
antimicrobial activity.
Supplementary Materials: The following are available online at https://www.mdpi.com/2223-774
7/10/2/252/s1. Table S1: GC-MS based analysis of chemical composition of various plant parts of
Avicennia marina plant by the various solvents.
Author Contributions: Conceptualization, M.K.O., A.A.A., S.S.A.-a., M.A.A.-M., W.H.S.; Data cura-
tion, S.S.A.-a.; Formal analysis, M.K.O., S.S.A.-a., M.A.A.-M., W.H.S.; Funding acquisition, M.K.O.,
A.A.A., S.S.A.-a.; Investigation, A.A., M.A.A.-M.; Methodology, M.K.O., A.A.A., S.S.A.-a., W.H.S.;
Project administration, M.A.A.-M.; Supervision, M.K.O., A.A.A., A.A.; Writing—original draft,
M.K.O., A.A.A., S.S.A.-a., M.A.A.-M., W.H.S., A.A. All authors have read and agreed to the published
version of the manuscript.
Funding: This research received no external funding.
Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Acknowledgments: We extend our appreciation to the Dean of Scientific Research, King Saud
University, for funding the work through the research group project number RG-1439-63. The
authors also thanks the Deanship of Scientific Research and RSSU at King Saud University for their
technical support.
Conflicts of Interest: The authors declare no conflict of interest.
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