4. WBC Count Procedure 1. Mix the blood sample thoroughly. 2. Fill blood into white pipette at 0.5 mark. 3. Fill reagent add up into the pipette to 11 mark. 4. Shake the pipette on the vibrator for 1 min. 5. Discarded the first 3-4 drops. 6. Fill in the hemacytometer nicely. 7. Allow WBC to set down for 2-3 min. 8. Count 4 white squares under microscope 9. Calculate the WBC concentration.
5. 5 WBC Count 1 mm. W W 1 mm. R R 1 mm. R R R W W High 0.1 mm. 3 mm.
7. 7 WBC Count Calculation 1. Volume of 1 white square = 1 x 1 x 0.1 cu.mm. = 0.1 cu.mm. 2. Volume of 4 white square = 0.1 x 4 cu.mm. = 0.4 cu.mm. 3. In 0.4 cu.mm. the WBC count = N (counted No.) 4. In 1 cu.mm. the WBC count = N x 1 / 0.4 = N x 2.5 5. The dilution for WBC = 0.5 / 10 = 20 6. The final WBC count = N x 2.5 x 20 = 50 N (/cu.mm.)
8. 8 WBC Count Calculation White cell count = number of cells counted (N) x volume factor (=2.5) x dilution factor (=20) = N x 50 Normal range = 5,000 - 10,000 / cu.mm. 5 - 10 x 103 / cu.mm.