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TOPIC 8 BLOOD CELL COUNT.pptx
1. Topic 8:
Blood Cell Count
BT1103 HEMATOLOGY
DIPLOMA IN MLT
BAINUN DALI
ICAN COLLEGE
2. Learning outcome
Identify the component and function of Haemocytometer set and Neubauer
Counting Chamber
Able to Perform blood dilution, incubation and cell counting techniques.
Able to understand calculation for total red cell, total white cell and platelet
counts
State the precautions in cell counting
9. PROCEDURE FOR TOTAL RED CELL & PLATELET
COUNT
FILLING THE PIPETTE
1. Mix blood thoroughly
2. Use the RBC diluting pipette and
pipette filler
3. Blood is drawn up to the o.5 mark
4. THE diluting fluid is then drawn up to
the 101 mark ( this gives a dilution of
1/200)
5. Mix thoroughly for at least 2-3 minutes
10. RED BLOOD CELL DILUTING FLUID
formal citrate
-sodium citrate: 3.0gm
-formaldehyde/formalin: 1.0ml
-distilled water: 100ML
*Any isotonic solution are acceptable such as normal
saline
11. Counting the rbc or/and
platelet
1. prepare the counting
chamber and the cover slip
2. charge one of the counting
areas
3. the fluid will fill up the space
by capillary action, DO NOT
OVERFILL
4. allow a few minutes for the
cells to settle
12. TRIPLE RULING METHOD
-to avoid duplication in counting cell
that touch the lines triple ruling is
used
cells that touch top and left central
lines are counted.
Cells that touch bottom and right
central lines are not counted
18. PROCEDURE WBC COUNT
Filling the pipette using the wbc pipette
Draw blood to 0.5 mark and follow up by diluting
fluid up to 11 mark (this give a dilution of 1/20)
19. Counting the white blood cell
1. locate the 4 large corner square, marked
w under 4x objective
2. Count using the triple rulling method
21. CALCULATION
RED BLOOD CELL: (a + b + C + d + e) /5 * (200) *
(0.004mm³/4 𝑥 10−6
cm³)
:_________ 4 𝑋 10−6 cm³ cell per cubic cm
White blood cell: (a + B + c + D) /4 * (20) * (0.1 mm³ /10−4 cm³ )
:__________X 10−4 cm³ cell per cubic cm
PLATELET COUNT: (All small squares) / 400 * (20) * (0.00025MM³
/ 2.5 x 10−7
𝐶𝑀³)
:_______________ / 2.5 x 10−7
𝐶𝑀³) cell per cubic cm
22. Precaution during cell counting
Clean hemocytometer, coverslip and pipette
Diluting fluid need to be contaminant-free
Adequate mixing to prevent clumping-platelet
Presence of air bubbles in pipette
Excess blood from the pipette tips