This study investigated how dental pulp cells and osteoblasts respond to gold substrates with different micropattern features. The researchers designed masks to create dot, line, hole, and hexagon patterns of varying sizes on gold films sputtered onto silicon wafers. Characterization with profilometry, AFM, and EDAX showed that film thickness and surface roughness increased with thicker films. After coating the patterns with an amino group and depositing cells, fluorescence imaging indicated the micropatterns were non-cytotoxic and osteoblasts attached normally. Future work will examine how cell behavior differs across the pattern features.
Joint Replacement: The Current and Future Impact of CoatingsApril Bright
The control of surface properties to reduce wear and corrosion and improve biocompatibility is of particular interest today as device companies—and surgeons, payors and patients—seek to extend the life of knee and hip implants. In this session, device companies shared research on their joint replacement coatings and materials, covering pros, cons and the future of their technology.
Coatings: The Power of Bone Integration to Aid Recovery - OMTEC 2018April Bright
Dr. Michael Gentile, DPM, fellow of the American College of Foot and Ankle Surgeons, lends his perspective on future coating technologies and ways to engage coating’s important role in a device. Special focus is given to titanium-integrated PEEK surfaces.
Antimicrobial Coatings: The Research and Regulatory PerspectiveApril Bright
Coatings have long been considered an avenue for infection prevention in orthopedic procedures. These coatings, some of which utilize silver, have largely not been commercialized because regulators seek greater evidence of their safety, creating a long, expensive road for device companies. Announcements in the last half of 2018 and early 2019 indicate that companies continue to push to get them on the market and that productive conversations are taking place with regulators. This session began with a history of antimicrobial coatings followed by a look at recent research and technology.
Joint Replacement: The Current and Future Impact of CoatingsApril Bright
The control of surface properties to reduce wear and corrosion and improve biocompatibility is of particular interest today as device companies—and surgeons, payors and patients—seek to extend the life of knee and hip implants. In this session, device companies shared research on their joint replacement coatings and materials, covering pros, cons and the future of their technology.
Coatings: The Power of Bone Integration to Aid Recovery - OMTEC 2018April Bright
Dr. Michael Gentile, DPM, fellow of the American College of Foot and Ankle Surgeons, lends his perspective on future coating technologies and ways to engage coating’s important role in a device. Special focus is given to titanium-integrated PEEK surfaces.
Antimicrobial Coatings: The Research and Regulatory PerspectiveApril Bright
Coatings have long been considered an avenue for infection prevention in orthopedic procedures. These coatings, some of which utilize silver, have largely not been commercialized because regulators seek greater evidence of their safety, creating a long, expensive road for device companies. Announcements in the last half of 2018 and early 2019 indicate that companies continue to push to get them on the market and that productive conversations are taking place with regulators. This session began with a history of antimicrobial coatings followed by a look at recent research and technology.
A Study on Effect of Sizes of aggregates on Steel Fiber Reinforced ConcreteIJERD Editor
Plain, unreinforced concrete is a brittle material, with a low tensile strength, limited ductility and
little resistance to cracking. In order to improve the inherent tensile strength of concrete there is a need of
multidirectional and closely spaced reinforcement, which can be provided in the form of randomly distributed
fibers. Steel fiber is one of the most commonly used fibers The present experimental study considers the effect
of aggregate size and steel fibers on the modulus of elasticity of concrete. Crimped steel fibers at volume
fraction of 0%.0.5%, 1.0% and 1.5% were used. Study on effect of volume fraction of fibers and change of
aggregate size on the modulus of elasticity of concrete was also deemed as an important part of present
experimental investigation. This work aims in studying the mechanical behavior of concrete in terms of modulus
of elasticity with the change of aggregate size reinforced with steel fibers of different series for M30 and M50
grade concretes. The results obtained show that the addition of steel fiber improves the modulus of elasticity of
concrete. It was also analyzed that by increasing the fiber volume fraction from 0%, to 1.5% there was a healthy
effect on modulus of elasticity of Steel Fiber Reinforced concrete.
Artículo científico sobre regeneración ósea con OsteoSynt. Imágenes de microscopio electrónico, histologías,
radiografías analizando como los sustitutos óseos de origen sintético favorecen la regeneración de hueso.
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
The future of dentistry and periodontics lies in regeneration. The goals of periodontal therapy lies in not only the arrest of periodontal disease progression but also regeneration of the lost periodontal structures. This presentation provides a review of the current understanding of the regeneration of the periodontium and the procedures involved to restore the periodontal tissues around the teeth.
Violência urbana criminalidade e tráfico de drogas uma discussao psicanalitic...Leonardo Faria
Violência urbana, criminalidade e tráfico de drogas: uma discussão psicanalítica acerca da adolescência. Um estudo que diz respeito ao desenvolvimento psicológico de adolescentes em conflito com a lei, e a relação com ausência paterna. Levando em consideração a teoria psicanalítica
A Study on Effect of Sizes of aggregates on Steel Fiber Reinforced ConcreteIJERD Editor
Plain, unreinforced concrete is a brittle material, with a low tensile strength, limited ductility and
little resistance to cracking. In order to improve the inherent tensile strength of concrete there is a need of
multidirectional and closely spaced reinforcement, which can be provided in the form of randomly distributed
fibers. Steel fiber is one of the most commonly used fibers The present experimental study considers the effect
of aggregate size and steel fibers on the modulus of elasticity of concrete. Crimped steel fibers at volume
fraction of 0%.0.5%, 1.0% and 1.5% were used. Study on effect of volume fraction of fibers and change of
aggregate size on the modulus of elasticity of concrete was also deemed as an important part of present
experimental investigation. This work aims in studying the mechanical behavior of concrete in terms of modulus
of elasticity with the change of aggregate size reinforced with steel fibers of different series for M30 and M50
grade concretes. The results obtained show that the addition of steel fiber improves the modulus of elasticity of
concrete. It was also analyzed that by increasing the fiber volume fraction from 0%, to 1.5% there was a healthy
effect on modulus of elasticity of Steel Fiber Reinforced concrete.
Artículo científico sobre regeneración ósea con OsteoSynt. Imágenes de microscopio electrónico, histologías,
radiografías analizando como los sustitutos óseos de origen sintético favorecen la regeneración de hueso.
IOSR Journal of Dental and Medical Sciences is one of the speciality Journal in Dental Science and Medical Science published by International Organization of Scientific Research (IOSR). The Journal publishes papers of the highest scientific merit and widest possible scope work in all areas related to medical and dental science. The Journal welcome review articles, leading medical and clinical research articles, technical notes, case reports and others.
The future of dentistry and periodontics lies in regeneration. The goals of periodontal therapy lies in not only the arrest of periodontal disease progression but also regeneration of the lost periodontal structures. This presentation provides a review of the current understanding of the regeneration of the periodontium and the procedures involved to restore the periodontal tissues around the teeth.
Violência urbana criminalidade e tráfico de drogas uma discussao psicanalitic...Leonardo Faria
Violência urbana, criminalidade e tráfico de drogas: uma discussão psicanalítica acerca da adolescência. Um estudo que diz respeito ao desenvolvimento psicológico de adolescentes em conflito com a lei, e a relação com ausência paterna. Levando em consideração a teoria psicanalítica
Transtorno de oposicao e desafio de conduta os desfechos no tdah em adultosLeonardo Faria
Transtorno de oposição e desafio e transtorno de conduta: os desfechos no TDAH em adultos. Uma relação entre transtornos mentais em adultos quanto ao desenvolvimento mental
O cérebro autista a biologia da mente e sua implicação no comprometimento socialLeonardo Faria
Este artigo discute sobre as diversas áreas do conhecimento em relação aos estudos do autismo, e os tipos de tratamentos realizados em pacientes, observando a singularidade de cada indivíduo.
Hadoop has become a backbone of many enterprises. While it can do wonders for businesses, it sometimes can be overwhelming for its operators and users. Amateurs as well as seasoned operators of Hadoop are caught unaware by common pitfalls of deploying, tuning and operating a Hadoop cluster. Having spent 5+ years working with 100s of Hadoop users, running clusters with 1000s of nodes, managing 10s of petabytes of data and running 100s of 1000s of tasks per day, we have seen people's unintentional acts, suboptimal configurations and common mistakes have resulted into downtimes, SLA violations, many hours of recovery operations and in some cases even data loss! Most of these traumas could have been easily avoided by applying easy to follow best practices that would protect data and optimize performance. In this talk we present real life stories, common pitfalls and most importantly, strategies on how to correctly deploy and manage Hadoop clusters. The talk will empower users and help make their Hadoop journey more fulfilling and rewarding. We will also discuss SmartSense. SmartSense can identify latent problems in a cluster and provide recommendations so that an operator can fix them before they manifest as a service degradation or outage.
Impact of dental implant surface modifications on Osseo-integrationNaveed AnJum
implant macro design as well as the surface topography plays an important role in higher survival rates of implants, especially in poor bone quality or density. Various modifications in surface topography have been enumerated here.
Class on "Porcelain layering on zirconia coping"
Presentation by Prof. Dr. Marco Ferrari MD, DMD, PhD.
http://www.dentalevo.it/dentistry-materials/porcelain-layering-zirconia-coping/
EXPERIMENTAL SUBSTANTIATION OF THE CHOICE OF THE RESTORATION METHOD IN THE CE...IAEME Publication
The etiology of abfraction defects is still in dispute. The etiology of these affections
is not fully established. An important factor in the success of the treatment of
abfraction defects of teeth by aesthetic restoration is the optimal choice of filling
materials. It is impossible to improve the efficiency and quality of dental care without
materials science. The aim of the study was to determine the strength of the
restoration with uniaxial compression of the tooth before and after thermal cycling.
The object of the study was 20 removed teeth with abfraction defects. The teeth were
divided into two groups depending on the type of restoration. Half of the teeth were
subjected to uniaxial loading in each group, the second half was subjected to thermal
cycling before loading. Samples with indirect restorations showed greater strength
both before and after thermal cycling.
FINITE ELEMENT ANALYSIS AND STATIC LOADING IN DENTAL IMPLANT WITH INNOVATIVE ...indexPub
Objective: The purpose of this study is to examine how different crown materials affect stress transformation and distribution around both dental implants. To do this, integrated and combined crowns were built, modelled, and tested under static axial loads using various material combinations. The biomechanical response was then examined. Methods: A validated three-dimensional finite element (FE) models of crown supported by implant was developed by to evaluate the effect of the different type of material (E max, zirconia, composite) on the short implant. After the FE models had been validated, simulations utilizing various configurations of various crowns fixed to two distinct types of implants were run and subjected to static loading to ascertain the distribution of stresses inside the bone around the implants. Result: The comparative results showed that manufacturing the crown using softer material (i.e., materials with lower elastic modulus) reduced the stress distribution in crown, implant and cancellous bone. It may refer to this phenomenon that softer material can absorb more energy from the applied compressive load, and result in transferring less energy to the implant and jaw bone. However, this effect was not significant on cortical bone compared to the cancellous bone. Combination of different materials for design and manufacturing the crown can alter the biomechanical response and could be beneficial for decreasing the stress distribution in implant and spongy region of jaw bone when stiffer material is needed to be covered in upper surface of the crown. In addition, the results suggests that shorter implant can increase the stress distribution in both cortical and cancellous bone. Conclusion: by using stiff material the stress will increase on the parts of implant and the surrounding bone which may led to failure of implant or bone resorption around of the implant, in other way by using less stiffer material the possibility of success will be increased and also the success rate of the implant is increased, also before deciding which implant size and length are used you select which type of the prosthetic will be used.
Die space to be maintained for the proper application of the spacer to maintain the contour and anatomy. in vitro studies were conducted to manage before applying in the patients study group. To attain axial,buccal, lingual and mesio-distal contours of the preparing tooth.
Influence of abutment material on the gingival color of implant-supported all...elenaAbcdental
Purpose: The aim of this clinical research on implant-supported restorations is to analyze, through
spectrophotometric digital technology, the influence of the abutment material on the color of the
peri-implant soft tissue.
Material and methods: Twenty patients received an endosseous dental implant in the anterior
maxilla. At the time of each definitive prosthesis delivery, an all-ceramic crown has been tried on gold,
titanium and zirconia abutment. After the insertion of each single abutment, the peri-implant soft
tissue color has been measured through a spectrophotometer. Also, the thickness of the facial periimplant
soft tissue was measured at the level of the implant neck through a caliper. A specific software
has been utilized to identify a specific tissue area and to collect the data before the statistical analysis
in Labn color space. The normality of the quantitative variables was verified by means of the Shapiro–
Wilk test. Simple linear correlation between quantitative variables was evaluated by using Pearson’s
coefficient. The results on the performance of the abutment materials with regard to the color
measurements and the overall measurement DE were described by computing the least-square means.
The significance of differences among types of abutment was verified by means of the Scheffe test for
multiple comparisons.
Results: For all the abutments used, the color of the peri-implant soft tissue appeared to be
significantly different from the one of the contra-lateral tooth (DE48.5). Significantly higher (Po0.05)
difference were present with the use of titanium abutments (11 " 0.4) when compared with the
results of gold (8.9 " 0.4) and zirconia (8.5 " 0.4) abutments. No correlation has been demonstrated
between soft tissue thickness and degree of color difference (P40.25).
Conclusions: Within the limitation of the present study, the peri-implant soft tissue color appears to
be different from the soft tissue color around natural teeth, no matter which type of restorative
material is selected. When titanium abutment was selected, significantly higher differences were
present than those obtained with gold or zirconia abutments. The thickness of the peri-implant soft
tissue did not appear to be a crucial factor in the abutment impact on the soft tissue color.
Dental Implants have changed the face of dentistry over the last 25 years. What are dental implants? What is the history of dental implants? And how are they used to replace missing teeth? This section will give you an overview of the topic of dental implants, to be followed by more detail in additional sections.
As with most treatment procedures in dentistry today, dental implants not only involve scientific discovery, research and understanding, but also application in clinical practice. The practice of implant dentistry requires expertise in planning, surgery and tooth restoration; it is as much about art and experience as it is about science. This site will help provide you with the knowledge you need to make informed choices in consultation with your dental health professionals.
Dental Implants
Dental illustration by Dear Doctor
Let’s start from the beginning: A dental implant is actually a replacement for the root or roots of a tooth. Like tooth roots, dental implants are secured in the jawbone and are not visible once surgically placed. They are used to secure crowns (the parts of teeth seen in the mouth), bridgework or dentures by a variety of means. They are made of titanium, which is lightweight, strong and biocompatible, which means that it is not rejected by the body. Titanium and titanium alloys are the most widely used metals in both dental and other bone implants, such as orthopedic joint replacements. Dental implants have the highest success rate of any implanted surgical device.
Titanium’s special property of fusing to bone, called osseointegration (“osseo” – bone; “integration” – fusion or joining with), is the biological basis of dental implant success. That’s because when teeth are lost, the bone that supported those teeth is lost too. Placing dental implants stabilizes bone, preventing its loss. Along with replacing lost teeth, implants help maintain the jawbone’s shape and density. This means they also support the facial skeleton and, indirectly, the soft tissue structures — gum tissues, cheeks and lips. Dental implants help you eat, chew, smile, talk and look completely natural. This functionality imparts social, psychological and physical well-being.
1. Response of Dental Pulp and Osteoblast Cells to Substrate Morphology for Applications in Dental Reconstruction G. Chen1 , M. Kennedy1 , D. Dean2 1 School of Materials Science and Engineering, Clemson University 2 Department of BioEngineering, Clemson University 2/8/2011
5. They are very similar in differentiation and protein expression.Optical image of human osteoblast cells (Saos-2 ) Image is from ATCC. http://www.atcc.org/Attachments/1995.jpg
6. Previous Studies on Cell Response to Micropatterns [1]Walboomers, X.F., Ginsel, L.A., Jansen, J.A. Early spreading events of fibroblasts on micro grooved substrates. J. Biomed. Mater. Res. 2000, 51, 529. [2]M. Winkelmanna, J. Goldb, R. Hauertc, B. Kasemob, N.D. Spencera, D.M. Brunetted, M. Textor . Chemically patterned, metal oxide based surfaces produced by photolithographic techniques for studying protein– and cell–surface interactions I: Micro fabrication and surface characterization. Biomaterials 24, 2003, 1133–1145. [3]Matthew J. Dalby, Mathis O. Riehle, Stephen J. Yarwood, Chris D.W. Wilkinson, Adam S.G. Curtisa. Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography. Experimental Cell Research, 2003, 284. [4]Schmidt JA, von Recum AF. Texturing of polymer surfaces at the cellular level. Biomaterials 1991; 12: 385-9.
7. Previous Studies on Cell Response to Chemistry [1]M. Andreiotelli, et al., "Are ceramic implants a viable alternative to titanium implants? A systematic literature review," 2009, pp. 32-47. [2]I. Abrahamsson and G. Cardaropoli, "Peri-implant hard and soft tissue integration to dental implants made of titanium and gold," Clinical Oral Implants Research, vol. 18, pp. 269-274, Jun 2007. [3]J. C. Setcos, et al., "The safety of nickel containing dental alloys," Dental Materials, vol. 22, pp. 1163-1168, Dec 2006. [4}A. C. L. Faria, et al., "In vitro cytotoxicity of dental alloys and cpTi obtained by casting," Journal of Biomedical Materials Research Part B-Applied Biomaterials, vol. 85B, pp. 504-508, May 2008. [5] H. X. Cheng, et al., "Effects of Surface Palladium/Ferrum Film on the Biocompatibility of 316L Stainless Steel," Rare Metal Materials and Engineering, vol. 37, pp. 2037-2040, Nov 2008. [6]K. Anselme and M. Bigerelle, "Effect of a gold-palladium coating on the long-term adhesion of human osteoblasts on biocompatible metallic materials," 2006, pp. 6325-6330. [7]A. Celebic, et al., “Ion release from gold/platinum dental alloy: could release of other elements be accountable in the contact allergy attributed to the gold?,” Journal of Materials Science-Materials in Medicine, vol. 17, pp. 301-305, Apr 2006.
9. Experimental Overview: Design Micropatterns Masks Culture Osteoblasts and Dental Pulp Cells Create Micropatterns and Characterize them with profilometer, AFM, EDAX Deposit Osteoblasts Deposit Dental Pulp Cells and track cell response.
10. Masks Design The masks were design by ICWIN program. Four masks were designed with feature size of 5 um, 10 um, 25 um and 50 um. Ordered from University of Minnesota.
11. Fabrication of Micropatterns and plating cells (a) 3 inch (100) silicon wafer (e) Applying amino group on top (b) Wafer with sputtered Au film (f)Culture osteoblast and dental pulp cells (c)Etch surface by photolithography Silicon wafer Gold film Amino group Cell (d) Sputter a 2nd layer of Au
12. Substrate matrix : Hexagon : Dot : Line : Hole Feature size: Diameter and width
13. AFM Image of Patterns Hole, 25 um Hexagon, 50 um Dot, 5 um Line, 10 um
14. Results: Film Thickness Parameters: RF power: 260 V Argon: 0.150 mmp Pressure: 1 *10-6torr Sputtering system in Kennedy’s lab
17. T.Y. Zhang, ect. Measurements of residual stresses in thin films deposited on silicon wafers by indentation fracture. Acta material, 1999, Vol. 47., No. 14, pp. 3869-3878.
18.
19. Actual Feature Size Green area Deflection (real size/target size)> 5%. Others are <5 %. Made the patterns we aimed for.
20. Surface roughness Roughness of Au Film increases with film thickness.
21. Surface Roughness vs. Scan Area film thickness/nm Matches results seen from other groups.
22. AFM Image of surface roughness 528 nm thick film. Height range: 50 nm 960 nm thick film. Height range: 50 nm Surface became rougher
23. Characterization of Au Grain Size Grain size was calculated by line- cross section method. (ASTM, E112 – 96 (Reapproved 2004)). 5 pictures for each sample were chosen. Grain Size increases with film thickness.
24. Surface change after applying amino group Purpose: Amino group lies between cells and patterns. Will it change morphology of patterns?
25. Characterization of Surface Morphology before and after applying amino group Holes, 25 um before applying amino group after applying amino group Surface morphology didn’t change much with amino group application.
26. Characterization of Surface Morphology before and after applying amino group Hexagon, 50 um before applying amino group after applying amino group Surface morphology didn’t change much with amino group application.
27. Cell culture Process Media: consists of MEM alpha (dipeptide L-Alanyl-L-Glutamine), pen/strep (Penicillin- Streptomycin), and FBS (Fetal bovine serum). DPBS: Dulbecco's Phosphate Buffered Saline
28. Initial Results of Plating Cells 100 um 100 um Fluorescence image of osteoblast vicilin and nuclei Fluorescence image of osteoblast vicilin Fluorescence image of osteoblast nuclei Au films with micropatterns are non- cytotoxic.
29.
30.
Editor's Notes
Change the back ground to simple.Add two images on the first slide…. Patterns and cell.
Add applications for objective. That is why it is important. Details on slides about overviewDelete some results, like chemistry, roughness and cell alignment, proliferation and whole discussion part
Orange boxes are this presentation’s topic
Schematically show the process and give objective of this study. Title is not proper. Preparing sample?
Show the film thicknesses, feature size and shapes.
Picture of sputtering system, process parameters onto slide…..
Finish defining constants and symbols used in equations.Get rid of mean and standard deviation. Just say that you measured six times.
Look up papers on film thickness and stress… should we be seeing this?Overall, there’s no common agreement on trend between film thickness and residual stress.In Brennan’s study, film thickness ranges from 0.79 um to 2.54 um. The results show that residual stress decreases with increasing film thickness. However, the differences are not distinguished [1]In another study, where the thickness was range from 36.5 nm to 84.5 nm, it is really hard to tell the trend in stress changing with film thickness. [2] Possible mechanism:roughness and tensile stress; rougher surfaces generate higher tensile stress due to the propensity for in-plane contact between neighboring grain surfaces. Doljack and Hoffman considered the stress developed when newly deposited grains are attracted to one another during deposition, causing grain coalescence or “zipping” of the grain boundaries. I haven't written them in the article as more tests are needed to investigate exact mechanism behind the results.[1] S. Brennan, ect. X-ray measurements of the depth dependence of stress in gold films. Physica B. 2000, pp. 125-129.[2] GregerThornell, ect. Residual stress in sputtered gold films on quarts measured by the cantilever beam deflection technique. IEEE transactions on ultrasonics, perroelectrus, and frequency control, 1999, Vol. 46, No.4
Put instrument that you used to determine the film roughness. Also how you did the measurements.
Fix scans
InsertaFM slide… how it works and how you will use it (tapping and contact mode)