The document explains the magnetic bead method nucleic acid extraction kit, detailing its principles, components, and specific steps for use. Nucleic acids, such as DNA and RNA, are crucial for genetic information storage and protein synthesis, while the extraction kit provides a safe, efficient process for isolating high-quality genomic DNA. The summarized steps include sample preparation, lysis, and purification, using magnetic beads and specific buffers to ensure a clean final product suitable for further experiments.

1. Magnetic bead method nucleic acid extraction kit
There are many types of nucleic acid extraction kits. Today, we will mainly introduce the principle,
use method and specific steps of the magnetic bead method nucleic acid extraction kit.
1. What is nucleic acid
Nucleic acids are widely present in all animals, plant cells, and microorganisms. Nucleic acids in
organisms often combine with proteins to form nucleoproteins.
According to different chemical composition, nucleic acid can be divided into ribonucleic acid,
referred to as RNA and deoxyribonucleic acid, referred to as DNA. DNA is the main material basis
for storing, copying and transmitting genetic information. RNA plays an important role in the
process of protein synthesis, among which transfer ribonucleic acid, tRNA for short. Plays the role
of carrying and transferring activated amino acids; messenger ribonucleic acid, referred to as
mRNA, is the template for protein synthesis. Ribosomal ribonucleic acid, rRNA for short, is the
main place where cells synthesize proteins.
2. What is the magnetic bead method nucleic acid extractionkit
The magnetic bead method nucleic acid extraction kit uses a unique separation of magnetic
beads and a buffer system to separate and purify high-quality genomic DNA from the sample.
Special coated magnetic beads have a strong affinity for the target DNA under certain conditions. ,
And when the conditions change, the magnetic beads release the adsorbed DNA, which can
achieve the purpose of rapid separation and purification of DNA. The whole process does not
involve toxic reagents, it is safe, convenient, and the extracted DNA is of high purity. The genomic
DNA (OD260-0D320/(0D280-0D320) purified by this kit is between 1.7 and 2.0, which can be
applied to various downstream molecular biology experiments.
The magnetic bead method nucleic acid extraction kit uses a unique separation of magnetic
beads and a buffer system to separate and purify high-quality genomic DNA from the sample.
Special coated magnetic beads have a strong affinity for the target DNA under certain conditions. ,
And when the conditions change, the magnetic beads release the adsorbed DNA, which can
achieve the purpose of rapid separation and purification of DNA. The whole process does not
involve toxic reagents, it is safe, convenient, and the extracted DNA is of high purity. The genomic
DNA (OD260-0D320/(0D280-0D320) purified by this kit is between 1.7 and 2.0, which can be
applied to various downstream molecular biology experiments.
Accounting extractionkit: DNA extraction kit, RNA extractionkit
Composition: Lysis solution, binding solution, washing solution, eluent, biomagnetic beads,
instructions
3. Use steps of magnetic bead method nucleic acid extractionkit:
1. Add Buffer C1, humic late adsorbent C2 and glass beads to the soil sample and vortex.
2. Add BufferC3 to lyse the cells
3. Add Buffer C4 to deform and precipitateprotein
4. Gaoxin removes impurities such as largehumic acid
5. Add BufferC 5. Ethanol to adjust the binding conditions
6. Transfer to a separation column and centrifuge to adsorb DNA
7. Add washing liquid to wash and remove impurities such as salt
8. Centrifuge for 1min to spin dry the base shield

2. 9. Add TE and centrifuge for 1 min to elute DNA
10. High-quality DNA
The above is the relevant introduction of the magnetic bead method nucleic acid extraction kit,
including the classification, composition, and specific use method steps of the nucleic acid
extraction kit.