Liquid Chromatography, introduction, principle , applications

1. HPLC Systems
Presented by
NIVEDITHA G
1st sem Mpharm
Dept of pharmaceutics
NARGUND COLLEGE OF PHARMACY 1

2. Column Chromatography
2

3. HPLC Modes
3

4. HPLC – System Components
4

5. HPLC – Pump Head
Piston HPLC Pump Head
5

6. Gradient
HPLC
High Pressure
Low Pressure
6

7. Chromatography – Dead Volume Mixing
7

8. HPLC
Ion
Separation
Mode
8

9. HPLC Solvents
Mobile Phase
9

10. HPLC – Solvents Properties
10

11. HPLC – Solvents Groups
11

12. HPLC – Solvents Polarity for Reverse Phase
12

13. HPLC – Solvent Effect
13

14. HPLC – Effect of Modifier
60:40 50:50 40:60
Acetonitrile : Water
14

15. HPLC – Gradient Curves
15

16. HPLC Columns
16

17. Mechanism of
separation in
different
forms of
HPLC
17

18. HPLC – Effect of Particles Size
18

19. HPLC – Particles Size
19

20. HPLC – Porous and Solid Packing
20

21. HPLC – Effect of Carbon Chain Length on Separation
21

22. HPLC – Column Diameter and Separation
22

23. Size Exclusion HPLC
23

24. HPLC Detectors

25. HPLC – Diode Array Detector
25

26. HPLC – UV/VIS Spectrophotometer
26

27. HPLC – Diode Array Detector Output
27

28. HPLC – UV/VIS Detector
28

29. HPLC – Spectrometric Detector Flow Cell Design
29

30. HPLC – UV Detector Cell
30

31. HPLC – Fluorescence Detector
31

32. HPLC - ELSD
32

33. HPLC - Evaporative Light Scattering Detector
33

34. HPLC - Detectors
34

35. HPLC – Derivatization for Detection
35

36. HPLC - Applications
36

37. 37