This document discusses high performance liquid chromatography (HPLC) systems. It covers HPLC components like the pump head, columns, solvents and detectors. Specifically, it examines piston pump heads, gradient curves, column packing materials, the effect of solvent composition and mobile phase polarity on separation. It also explores various detectors such as UV-Vis, diode array, fluorescence and evaporative light scattering detectors. The document aims to provide an overview of key aspects of HPLC systems and their applications in separation and analysis.

1. HPLC Systems
Presented by
NIVEDITHA G
1st sem Mpharm
Dept of pharmaceutics
NARGUND COLLEGE OF PHARMACY 1

2. Column Chromatography
2

3. HPLC Modes
3

4. HPLC – System Components
4

5. HPLC – Pump Head
Piston HPLC Pump Head
5

6. Gradient
HPLC
High Pressure
Low Pressure
6

7. Chromatography – Dead Volume Mixing
7

8. HPLC
Ion
Separation
Mode
8

9. HPLC Solvents
Mobile Phase
9

10. HPLC – Solvents Properties
10

11. HPLC – Solvents Groups
11

12. HPLC – Solvents Polarity for Reverse Phase
12

13. HPLC – Solvent Effect
13

14. HPLC – Effect of Modifier
60:40 50:50 40:60
Acetonitrile : Water
14

15. HPLC – Gradient Curves
15

16. HPLC Columns
16

17. Mechanism of
separation in
different
forms of
HPLC
17

18. HPLC – Effect of Particles Size
18

19. HPLC – Particles Size
19

20. HPLC – Porous and Solid Packing
20

21. HPLC – Effect of Carbon Chain Length on Separation
21

22. HPLC – Column Diameter and Separation
22

23. Size Exclusion HPLC
23

24. HPLC Detectors

25. HPLC – Diode Array Detector
25

26. HPLC – UV/VIS Spectrophotometer
26

27. HPLC – Diode Array Detector Output
27

28. HPLC – UV/VIS Detector
28

29. HPLC – Spectrometric Detector Flow Cell Design
29

30. HPLC – UV Detector Cell
30

31. HPLC – Fluorescence Detector
31

32. HPLC - ELSD
32

33. HPLC - Evaporative Light Scattering Detector
33

34. HPLC - Detectors
34

35. HPLC – Derivatization for Detection
35

36. HPLC - Applications
36

37. 37