Let\'s consider making a knockout of a gene. (a) What are two aspects of yeast that make generating gene knockouts relatively straight- forward? (b) What are two aspects of mouse that make generating gene knockouts relatively challenging? (c) If mouse knockouts are more challenging, why would you perform them? Solution two aspects of yeast that make generating gene knockouts relatively straight-forward 1]After exogenous or foreign yeast DNA is taken up by diploid yeast cells, recombination generally occurs between between the introduced DNA & the homologous chromosomal sites .in the recipient cells 2]Because of this specific ,targeted recombination of identical streches of DNA called homologous recombination 3] Any gene in a yeast chromosome can be replaced with mutant allele 4] The heterozygous yeast cell carring one mutant allele & one wild type allele generally grow normally (b) two aspects of mouse that make generating gene knockouts relatively challenging are 1] isolation & culturing of embryonic stem cells from inner cell mass of mouse blastocyst is easy 2] introduction of mutant or distrupted gene into cultured ES cells & selection of homologous recombinant cells in which the gene of interest has been knockout 3] used for studying the human inherited diseases c] mouse knockouts are more challenging but we perform them for studying the human inherited diseases powerfull tools for investigating the nature of genetic diseases For different types of treatments & gene theropies to cure diseases.

1. Let's consider making a knockout of a gene.
(a) What are two aspects of yeast that make generating gene knockouts relatively straight-
forward?
(b) What are two aspects of mouse that make generating gene knockouts relatively challenging?
(c) If mouse knockouts are more challenging, why would you perform them?
Solution
two aspects of yeast that make generating gene knockouts relatively straight-forward
1]After exogenous or foreign yeast DNA is taken up by diploid yeast cells, recombination
generally occurs between between the introduced DNA & the homologous chromosomal sites .in
the recipient cells
2]Because of this specific ,targeted recombination of identical streches of DNA called
homologous recombination
3] Any gene in a yeast chromosome can be replaced with mutant allele
4] The heterozygous yeast cell carring one mutant allele & one wild type allele generally grow
normally
(b) two aspects of mouse that make generating gene knockouts relatively challenging are
1] isolation & culturing of embryonic stem cells from inner cell mass of mouse blastocyst is easy
2] introduction of mutant or distrupted gene into cultured ES cells & selection of homologous
recombinant cells in which the gene of interest has been knockout
3] used for studying the human inherited diseases
c] mouse knockouts are more challenging but we perform them for
studying the human inherited diseases
powerfull tools for investigating the nature of genetic diseases
For different types of treatments & gene theropies to cure diseases