Legionellosis is caused by the bacterium Legionella pneumophila. The bacteria typically contaminates hot water tubs, air conditioners, and mist sprayers. The bacterium grows best in warm waters. It is small, aerobic, water borne gram negative, catalase positive bacterium. The bacterium is an intracellular parasite. It can infect protozoa and phagocytic cells. They resist phagolysosome formation and thus, avoid intracellular killing. They multiply inside the phagocytic cell, especially macrophages and monocytes. The exact mechanism and virulence factors responsible for pathogencity are not yet found. Hence, there is no vaccine available yet against this infection. PCR based assays are done to determine serotype, using 16S rRNA specific probes, and probes specific for virulence assocaited genes. Solution Legionellosis is caused by the bacterium Legionella pneumophila. The bacteria typically contaminates hot water tubs, air conditioners, and mist sprayers. The bacterium grows best in warm waters. It is small, aerobic, water borne gram negative, catalase positive bacterium. The bacterium is an intracellular parasite. It can infect protozoa and phagocytic cells. They resist phagolysosome formation and thus, avoid intracellular killing. They multiply inside the phagocytic cell, especially macrophages and monocytes. The exact mechanism and virulence factors responsible for pathogencity are not yet found. Hence, there is no vaccine available yet against this infection. PCR based assays are done to determine serotype, using 16S rRNA specific probes, and probes specific for virulence assocaited genes..