This document discusses fluorescence probes and labels for biomedical applications. It begins by classifying fluorophores as either intrinsic, which occur naturally, or extrinsic, which are synthetic dyes added to specimens. Intrinsic fluorophores discussed include aromatic amino acids like tryptophan in proteins, as well as enzyme cofactors and porphyrins. Extrinsic fluorophores discussed include organic dyes, metal-ligand complexes, quantum dots, and fluorescent proteins. The document also covers topics like choosing fluorescent probes based on properties like dissociation constants, calcium probes like Fura-2, and labeling biomolecules with fluorescent labels like fluorescein and rhodamine dyes.
The only comprehensive database on natural products.
DNP organize, simplify and bring out the underlying structural and biosynthetic relationships of the compounds.DNP provides access to chemical, physical, and structural data on hundreds of thousands of natural products
Deformulating Complex Polymer Mixtures By GPC-IR Technologymzhou45
This document summarizes a presentation on using hyphenated GPC-IR technology to de-formulate complex polymer mixtures. The presentation discusses the DiscovIR-LC system and its features for GPC-IR and HPLC-IR analysis. It provides several case studies demonstrating how GPC-IR can be used to identify individual polymer components, characterize copolymer compositions, analyze polymer additives and degradation, and more. The speaker concludes that GPC-IR is a powerful tool for de-formulating complex polymer systems and problem solving in various industries.
NYSAS Seminar LC-IR To Characterize Polymeric Excipients In Pharmaceutical F...mzhou45
This document describes an LC-IR technique for characterizing polymeric excipients in pharmaceutical formulations. The LC-IR system combines liquid chromatography separation with online infrared spectroscopy detection. It is used to characterize copolymer compositions, detect excipient degradation from hot melt extrusion processes, and study the stability of excipients like PEG. The LC-IR technique provides compositional information and identifies degradation products with molecular weight distributions. This allows understanding of excipient properties, degradation mechanisms, and process effects to ensure quality and stability of pharmaceutical formulations.
Layer-by-layer (LbL) films have been produced with
poly(o-ethoxyaniline) (POEA), chitosan and chitosanpoly(
methacrylic acid) (CS-PMAA) nanoparticles. Because the
adsorption of LbL films depends on ionic interactions and Hbonding, optimized conditions had to be established for the growth of multilayer films. Unusually thick films were obtained for POEA and CS-PMAA, thus demonstrating the importance of using chitosan in the form of nanoparticles.
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...mzhou45
GPC-IR combined technique to characterize polymeric excipients for lot-to-lot variations and degradation/stability from thermal processing in drug formulations
LC-IR Applications In Polymer Related Industriesmzhou45
LC-IR Application Overview for Polymer Related Industries with Many Case Studies: characterize copolymer compositions across MWD and de-formulate complex polymer mixtures
The only comprehensive database on natural products.
DNP organize, simplify and bring out the underlying structural and biosynthetic relationships of the compounds.DNP provides access to chemical, physical, and structural data on hundreds of thousands of natural products
Deformulating Complex Polymer Mixtures By GPC-IR Technologymzhou45
This document summarizes a presentation on using hyphenated GPC-IR technology to de-formulate complex polymer mixtures. The presentation discusses the DiscovIR-LC system and its features for GPC-IR and HPLC-IR analysis. It provides several case studies demonstrating how GPC-IR can be used to identify individual polymer components, characterize copolymer compositions, analyze polymer additives and degradation, and more. The speaker concludes that GPC-IR is a powerful tool for de-formulating complex polymer systems and problem solving in various industries.
NYSAS Seminar LC-IR To Characterize Polymeric Excipients In Pharmaceutical F...mzhou45
This document describes an LC-IR technique for characterizing polymeric excipients in pharmaceutical formulations. The LC-IR system combines liquid chromatography separation with online infrared spectroscopy detection. It is used to characterize copolymer compositions, detect excipient degradation from hot melt extrusion processes, and study the stability of excipients like PEG. The LC-IR technique provides compositional information and identifies degradation products with molecular weight distributions. This allows understanding of excipient properties, degradation mechanisms, and process effects to ensure quality and stability of pharmaceutical formulations.
Layer-by-layer (LbL) films have been produced with
poly(o-ethoxyaniline) (POEA), chitosan and chitosanpoly(
methacrylic acid) (CS-PMAA) nanoparticles. Because the
adsorption of LbL films depends on ionic interactions and Hbonding, optimized conditions had to be established for the growth of multilayer films. Unusually thick films were obtained for POEA and CS-PMAA, thus demonstrating the importance of using chitosan in the form of nanoparticles.
New LC-IR Technique To Characterize Polymeric Excipients In Pharmaceutical Fo...mzhou45
GPC-IR combined technique to characterize polymeric excipients for lot-to-lot variations and degradation/stability from thermal processing in drug formulations
LC-IR Applications In Polymer Related Industriesmzhou45
LC-IR Application Overview for Polymer Related Industries with Many Case Studies: characterize copolymer compositions across MWD and de-formulate complex polymer mixtures
This document outlines the development of an aptamer-based MRI contrast agent for thrombin detection. The goals of the project are to synthesize and purify an aptamer-chelate conjugate and screen contrast agents to find the best system for measuring thrombin. Initial results showed successful aptamer synthesis but mass spectrometry revealed the conjugation reaction was not efficient. Future work will involve reacting the aptamer with DTPA or DOTA chelates and testing the conjugates in MRI.
The final presentation for BTC1700H for team Wild Type. The presentation includes financial and clinical data regarding expression and purification of GFP from an unknown sample of DNA.
The document describes the development of an aptamer-based MRI contrast agent for detecting thrombin. It discusses using an aptamer that binds to thrombin along with gadolinium-chelator conjugates DOTA or DTPA. The project aims to synthesize and purify aptamer-chelate conjugates, and potentially screen contrast agents to measure thrombin in serum. Initial results showed the aptamer was successfully synthesized when conjugated to DOTA or DTPA via PAGE. However, mass spectrometry revealed the conjugates were not synthesized efficiently, as the observed masses were too small compared to theoretical values.
The document describes a study aiming to develop an MRI contrast agent targeted to thrombin for precise imaging of blood clots. An aptamer known to bind thrombin was synthesized and conjugated to gadolinium chelates DTPA and DOTA. Initial mass spectrometry results showed the conjugates did not form. After further refining synthesis and purification methods, conjugates showed improved yield and purity. The targeted contrast agent could enable more precise MRI imaging of internal bleeding and blood clotting than current invasive techniques.
HPLC2010 Characterization Of Short Chain Branched Polyolefins By GPC-FTIRmzhou45
This document discusses characterization of short chain branched polyolefins using size exclusion chromatography coupled with Fourier transform infrared spectroscopy (SEC/GPC-FTIR). It describes how the hyphenated SEC/GPC-FTIR system works by removing solvent after SEC/GPC separation and depositing the dry analytes directly onto an infrared detector. The document provides examples of using this technique to analyze short chain branching in ethylene copolymers and de-formulate polyolefin mixtures.
The document describes research into developing new ligands that bind to the α2δ1 subunit of voltage-gated calcium channels for the treatment of neuropathic pain. Several chemical series were discovered that showed binding affinity for α2δ1 including pyrrolopyridazines, triazolophthalazines, and N-acridinylbutanediamines. The researchers aimed to improve potency, metabolic stability, and physicochemical properties. High-throughput methods identified new analogs and established that binding was specific to the α2δ1 target.
Ultraviolet resonance raman study of the avidin biotin complexJohn Clarkson
J. Clarkson*, C. Sudworth, S.I. Masca, D.N. Batchelder & D.A. Smith, “Ultraviolet resonance Raman study of the avidin biotin complex”, J. Raman Spectrosc., 31, 373-375, 2000.
Getting the Big Picture by Joining up the SAR dotsSorel Muresan
Getting the Big Picture by Joining up the SAR dots
This document discusses challenges in integrating structure and bioactivity data at large scales due to the volume and complexity of unstructured data from various sources. It describes efforts to extract chemical entities from text using natural language processing and to standardize structures. The Chemistry Connect knowledge base aims to enable searching across internal and external datasets by developing a chemical dictionary and common representation of concepts.
This document presents a comparative study of uranium(VI) extraction between three resins: Lewatit TP 260, polyethyleniminephenylphosphonamidic acid (PEIPPA), and diaminotriphosphonic acid grafted polystyrene divinyl benzene (DATPA). Batch studies were conducted to optimize parameters such as pH, contact time, initial uranium concentration, ion strength, and temperature. The results showed that PEIPPA extracts uranium best at high acidity (pH ~1.5), while DATPA extracts effectively at low acidity (pH ~3.5). Sorption capacities for uranium were 39.66 mg/g for PEIPPA,
This document summarizes research on using porphyrin nanostructures for artificial photosynthesis applications. Key points include:
- Porphyrin nanotubes and "micro-clovers" self-assemble from ionic interactions between positively and negatively charged porphyrins.
- The nanostructures exhibit light harvesting properties and can generate hydrogen when combined with platinum nanoparticles and an electron donor/acceptor system.
- The morphology of the porphyrin structures can be tuned by varying growth conditions like ionic strength and temperature.
- The researchers propose hybrid artificial photosynthesis systems that combine porphyrin nanostructures with semiconductor nanoparticles to more efficiently split water and generate solar fuels using visible light
ALFA is a novel fluorescence tool that provides unprecedented accuracy in measuring chlorophyll, CDOM, variable fluorescence (Fv/Fm), and phytoplankton pigments in natural waters. It utilizes two lasers at 405nm and 515nm to stimulate fluorescence and corrects for CDOM interference. Spectral deconvolution of emission spectra allows for quantification of individual pigments. ALFA operates in both discrete bottle sampling mode and continuous flow-through mode aboard ships. It provides a wealth of information on phytoplankton communities, biogeochemistry, and physiology.
Patent Cheminformatics: Identification of key compounds in patentsSorel Muresan
Patents can contain valuable chemical and biological information not found in scientific journals. This document discusses extracting key compounds from patents, including identifying sources for full-text patents, extracting compounds from text, and predicting key compounds through methods like frequency of group analysis. Predicting key compounds is important as they are often the most biologically active and suitable for further development. The document provides examples of extracting known drug compounds like Bextra, Aciphex, and Aricept from early patents through these methods.
Deep Purple: Discolouration in CBD productsMarkus Roggen
Presentation at ACS Spring 2022 conference.
Recently, there has been a flood of cannabidiol (CBD)-containing products, with divers marketing claims for a plethora of use cases. With this new market segment, regulatory oversight is still developing, and label claims of CBD concentration is often verifiable. CBD is unstable in solution and some CBD products are anecdotally reported to turn purple on storage; however, the decomposition products of CBD are mostly unknown.
Delic Lab embarked on a long and painstaking hunt for those decomposition products, established their identity through complementary chemical methods and established reaction pathways between them.
We will present our findings about common CBD oxidation products, who those are highly photochemically unstable and decomposes rapidly. Decomposition leads to a multitude of new cannabinoid derivatives.
Automated spelling correction to improve recall rates of name-to-structure to...Sorel Muresan
This document discusses using automated spelling correction to improve the recall rates of name-to-structure tools for chemical text mining. It notes that the biggest cause of missing compounds when extracting chemical entities is the presence of typographical errors. The document introduces CaffeineFix, a chemical nomenclature aware automatic spell checker, that can significantly improve recall rates as a pre-processing step by correcting OCR and other errors in compound names found in patents and other texts.
This document summarizes research on strontium iodide scintillator materials conducted by Lawrence Livermore National Laboratory. It finds that SrI2 doped with europium is a promising scintillator that offers high light yield and energy resolution comparable to lanthanum bromide. The document outlines thermal and optical properties of SrI2 and describes crystal growth techniques. It presents results demonstrating less than 4% energy resolution at 662 keV can be achieved with encapsulated SrI2 crystals and analog readout.
Polylactide (PLA) (nano)composites were recently considered for utilization in engineering sectors. For targeted applications requiring flame retardant (FR) properties, PLA can be effectively modified with previously dehydrated gypsum (so-called CaSO4 β-anhydrite II, AII) and selected additives. As evidenced by cone calorimetry, the co-addition of AII and organo-modified layered silicates (OMLS) is leading to PLA (nano)composites showing the increase in ignition time and decrease with above 40% of pRHR, the maximum rate of heat release. Furthermore, advanced FR properties, i.e., the category V0 (UL 94V), can be achieved by co-addition into PLA of AII and halogen free FR additives (melamine derivatives).
14th European Meeting on Fire Retardant Polymers
FRPM13, Lille, FRANCE
Craig Hawker of UCSB: Commercial Applications of Polymer as Nanomaterialsucsb.ira
The document discusses using polymers as nanomaterials for biomedical applications. It describes synthesizing multifunctional nanoparticles with a polyethylene glycol shell and reactive internal groups. The nanoparticles can be functionalized with targeting ligands on the surface and therapeutics internally. Radiolabeled nanoparticles showed long blood circulation times and accumulation in tumors, demonstrating their potential for drug delivery and imaging applications.
This document is a presentation on odors and toxicity from cooking fresh or decaying protein given by Stephen R. Temple and Howard E. Whitney of Steen Research LLC. It discusses the sources of odors and toxins as primary sources like cookers and secondary sources like evaporator condensate. It then examines the science behind these issues, identifying the various gas compounds released and their physical properties. Finally, it discusses engineering challenges and methods for advanced pre-treatment and cloud formation elimination to reduce these odors and toxins.
Downstream Processing in Biopharmaceuticalsangelsalaman
This document discusses downstream processing in biopharmaceuticals. It provides examples of characterizing proteins like human serum albumin, describing its molecular weight, isoelectric point, and hydropathicity. It also discusses large scale protein production methods like transfection, purification using affinity and size exclusion chromatography, and challenges like glycosylation that can be addressed through techniques such as mutagenesis and using insect cells. The typical downstream processing flow is outlined involving clarification, filtration, chromatography, and viral clearance steps.
This document outlines the development of an aptamer-based MRI contrast agent for thrombin detection. The goals of the project are to synthesize and purify an aptamer-chelate conjugate and screen contrast agents to find the best system for measuring thrombin. Initial results showed successful aptamer synthesis but mass spectrometry revealed the conjugation reaction was not efficient. Future work will involve reacting the aptamer with DTPA or DOTA chelates and testing the conjugates in MRI.
The final presentation for BTC1700H for team Wild Type. The presentation includes financial and clinical data regarding expression and purification of GFP from an unknown sample of DNA.
The document describes the development of an aptamer-based MRI contrast agent for detecting thrombin. It discusses using an aptamer that binds to thrombin along with gadolinium-chelator conjugates DOTA or DTPA. The project aims to synthesize and purify aptamer-chelate conjugates, and potentially screen contrast agents to measure thrombin in serum. Initial results showed the aptamer was successfully synthesized when conjugated to DOTA or DTPA via PAGE. However, mass spectrometry revealed the conjugates were not synthesized efficiently, as the observed masses were too small compared to theoretical values.
The document describes a study aiming to develop an MRI contrast agent targeted to thrombin for precise imaging of blood clots. An aptamer known to bind thrombin was synthesized and conjugated to gadolinium chelates DTPA and DOTA. Initial mass spectrometry results showed the conjugates did not form. After further refining synthesis and purification methods, conjugates showed improved yield and purity. The targeted contrast agent could enable more precise MRI imaging of internal bleeding and blood clotting than current invasive techniques.
HPLC2010 Characterization Of Short Chain Branched Polyolefins By GPC-FTIRmzhou45
This document discusses characterization of short chain branched polyolefins using size exclusion chromatography coupled with Fourier transform infrared spectroscopy (SEC/GPC-FTIR). It describes how the hyphenated SEC/GPC-FTIR system works by removing solvent after SEC/GPC separation and depositing the dry analytes directly onto an infrared detector. The document provides examples of using this technique to analyze short chain branching in ethylene copolymers and de-formulate polyolefin mixtures.
The document describes research into developing new ligands that bind to the α2δ1 subunit of voltage-gated calcium channels for the treatment of neuropathic pain. Several chemical series were discovered that showed binding affinity for α2δ1 including pyrrolopyridazines, triazolophthalazines, and N-acridinylbutanediamines. The researchers aimed to improve potency, metabolic stability, and physicochemical properties. High-throughput methods identified new analogs and established that binding was specific to the α2δ1 target.
Ultraviolet resonance raman study of the avidin biotin complexJohn Clarkson
J. Clarkson*, C. Sudworth, S.I. Masca, D.N. Batchelder & D.A. Smith, “Ultraviolet resonance Raman study of the avidin biotin complex”, J. Raman Spectrosc., 31, 373-375, 2000.
Getting the Big Picture by Joining up the SAR dotsSorel Muresan
Getting the Big Picture by Joining up the SAR dots
This document discusses challenges in integrating structure and bioactivity data at large scales due to the volume and complexity of unstructured data from various sources. It describes efforts to extract chemical entities from text using natural language processing and to standardize structures. The Chemistry Connect knowledge base aims to enable searching across internal and external datasets by developing a chemical dictionary and common representation of concepts.
This document presents a comparative study of uranium(VI) extraction between three resins: Lewatit TP 260, polyethyleniminephenylphosphonamidic acid (PEIPPA), and diaminotriphosphonic acid grafted polystyrene divinyl benzene (DATPA). Batch studies were conducted to optimize parameters such as pH, contact time, initial uranium concentration, ion strength, and temperature. The results showed that PEIPPA extracts uranium best at high acidity (pH ~1.5), while DATPA extracts effectively at low acidity (pH ~3.5). Sorption capacities for uranium were 39.66 mg/g for PEIPPA,
This document summarizes research on using porphyrin nanostructures for artificial photosynthesis applications. Key points include:
- Porphyrin nanotubes and "micro-clovers" self-assemble from ionic interactions between positively and negatively charged porphyrins.
- The nanostructures exhibit light harvesting properties and can generate hydrogen when combined with platinum nanoparticles and an electron donor/acceptor system.
- The morphology of the porphyrin structures can be tuned by varying growth conditions like ionic strength and temperature.
- The researchers propose hybrid artificial photosynthesis systems that combine porphyrin nanostructures with semiconductor nanoparticles to more efficiently split water and generate solar fuels using visible light
ALFA is a novel fluorescence tool that provides unprecedented accuracy in measuring chlorophyll, CDOM, variable fluorescence (Fv/Fm), and phytoplankton pigments in natural waters. It utilizes two lasers at 405nm and 515nm to stimulate fluorescence and corrects for CDOM interference. Spectral deconvolution of emission spectra allows for quantification of individual pigments. ALFA operates in both discrete bottle sampling mode and continuous flow-through mode aboard ships. It provides a wealth of information on phytoplankton communities, biogeochemistry, and physiology.
Patent Cheminformatics: Identification of key compounds in patentsSorel Muresan
Patents can contain valuable chemical and biological information not found in scientific journals. This document discusses extracting key compounds from patents, including identifying sources for full-text patents, extracting compounds from text, and predicting key compounds through methods like frequency of group analysis. Predicting key compounds is important as they are often the most biologically active and suitable for further development. The document provides examples of extracting known drug compounds like Bextra, Aciphex, and Aricept from early patents through these methods.
Deep Purple: Discolouration in CBD productsMarkus Roggen
Presentation at ACS Spring 2022 conference.
Recently, there has been a flood of cannabidiol (CBD)-containing products, with divers marketing claims for a plethora of use cases. With this new market segment, regulatory oversight is still developing, and label claims of CBD concentration is often verifiable. CBD is unstable in solution and some CBD products are anecdotally reported to turn purple on storage; however, the decomposition products of CBD are mostly unknown.
Delic Lab embarked on a long and painstaking hunt for those decomposition products, established their identity through complementary chemical methods and established reaction pathways between them.
We will present our findings about common CBD oxidation products, who those are highly photochemically unstable and decomposes rapidly. Decomposition leads to a multitude of new cannabinoid derivatives.
Automated spelling correction to improve recall rates of name-to-structure to...Sorel Muresan
This document discusses using automated spelling correction to improve the recall rates of name-to-structure tools for chemical text mining. It notes that the biggest cause of missing compounds when extracting chemical entities is the presence of typographical errors. The document introduces CaffeineFix, a chemical nomenclature aware automatic spell checker, that can significantly improve recall rates as a pre-processing step by correcting OCR and other errors in compound names found in patents and other texts.
This document summarizes research on strontium iodide scintillator materials conducted by Lawrence Livermore National Laboratory. It finds that SrI2 doped with europium is a promising scintillator that offers high light yield and energy resolution comparable to lanthanum bromide. The document outlines thermal and optical properties of SrI2 and describes crystal growth techniques. It presents results demonstrating less than 4% energy resolution at 662 keV can be achieved with encapsulated SrI2 crystals and analog readout.
Polylactide (PLA) (nano)composites were recently considered for utilization in engineering sectors. For targeted applications requiring flame retardant (FR) properties, PLA can be effectively modified with previously dehydrated gypsum (so-called CaSO4 β-anhydrite II, AII) and selected additives. As evidenced by cone calorimetry, the co-addition of AII and organo-modified layered silicates (OMLS) is leading to PLA (nano)composites showing the increase in ignition time and decrease with above 40% of pRHR, the maximum rate of heat release. Furthermore, advanced FR properties, i.e., the category V0 (UL 94V), can be achieved by co-addition into PLA of AII and halogen free FR additives (melamine derivatives).
14th European Meeting on Fire Retardant Polymers
FRPM13, Lille, FRANCE
Craig Hawker of UCSB: Commercial Applications of Polymer as Nanomaterialsucsb.ira
The document discusses using polymers as nanomaterials for biomedical applications. It describes synthesizing multifunctional nanoparticles with a polyethylene glycol shell and reactive internal groups. The nanoparticles can be functionalized with targeting ligands on the surface and therapeutics internally. Radiolabeled nanoparticles showed long blood circulation times and accumulation in tumors, demonstrating their potential for drug delivery and imaging applications.
This document is a presentation on odors and toxicity from cooking fresh or decaying protein given by Stephen R. Temple and Howard E. Whitney of Steen Research LLC. It discusses the sources of odors and toxins as primary sources like cookers and secondary sources like evaporator condensate. It then examines the science behind these issues, identifying the various gas compounds released and their physical properties. Finally, it discusses engineering challenges and methods for advanced pre-treatment and cloud formation elimination to reduce these odors and toxins.
Downstream Processing in Biopharmaceuticalsangelsalaman
This document discusses downstream processing in biopharmaceuticals. It provides examples of characterizing proteins like human serum albumin, describing its molecular weight, isoelectric point, and hydropathicity. It also discusses large scale protein production methods like transfection, purification using affinity and size exclusion chromatography, and challenges like glycosylation that can be addressed through techniques such as mutagenesis and using insect cells. The typical downstream processing flow is outlined involving clarification, filtration, chromatography, and viral clearance steps.
This document provides information on reverse osmosis, including its principles, typical arrangements, operating parameters, applications, and troubleshooting. It discusses the basics of RO including interfaces, pore size distribution, and applied pressure. Pretreatment requirements are outlined to prevent fouling and scaling. Common membrane types and manufacturers are listed. Methods for interpreting changes in operating parameters that may indicate issues like fouling or scaling are described. Finally potential RO chemicals from antiscalants to biocides to cleaners are presented.
Honeywell offer a wide range of Research Chemicals under their own brands Burdick & Jackson™, Fluka™, Honeywell™,Riedel-de-Haën™, Chromasolv™, Hydranal™, LabReady™ Blends, TraceSLECT™. Covering all applications, including, Bioanalysis, Biosynthesis, Chemical Synthesis, Chromatography, Electrophoresis, Karl Fischer Titration, Octane Determination, Spectroscopy, Titration, Trace Analysis and Wet Chemistry.
Established in 1981,Greyhound Chromatography has been supplying high quality Chromatography consumables to Research and Analysis Laboratories around the world for 38 years. Greyhound's Managing Director, Paul Massie founded the company which operates from its UK warehouse and office facility, located in Birkenhead, Merseyside.
Greyhound supplies Certified Reference Standards and Materials, Research Chemicals; including Solvents and Reagents and Laboratory consumables. New products are constantly added to Greyhound's e-commerce website, be sure to register to the website to view product prices.
Greyhound’s extensive range covers all areas of Environmental, Petrochemical, Food, Fragrance, Forensics, Chemical and Pharmaceutical analysis, holding stock of many popular products for prompt delivery via our extensive logistics network.
Greyhound prides itself on personal service which provides prompt, efficient, cost-effective, safe delivery of all products. With state-of-the art facilities and highly trained staff, Greyhound provides technical advice and distribution of Chromatography consumables across all disciplines. Our service is designed to provide a wide range of products, to help our clients to achieve excellent, cost-effective results. Greyhound manufactures its own range of Capillary Columns, Syringe Filters, SPE Cartridges and HPLC Columns, the 'Q' Range, as well as representing the industry’s best known manufacturers.
The document describes the analytical equipment available at a central lab unit, including various types of spectroscopy and chromatography instruments for analyzing metals, organic compounds, and other substances. Specifically, it mentions atomic absorption spectrometers, gas chromatographs, high performance liquid chromatography, spectrofluorometers, UV/Vis spectrophotometers, ICP-MS, XRD, and other instruments and their applications in environmental, food, and biological analysis.
1) Investigators developed analytical methods to detect trienone agricultural pharmaceuticals and their photoproducts at low concentrations in the environment.
2) Photolysis experiments showed these compounds transform into more polar and biologically active photoproducts that can revert back to the parent compound.
3) Column experiments demonstrated these photoproducts may have an increased risk of transport compared to the less polar parent compounds.
LC-MS is a hyphenated technique that combines liquid chromatography with mass spectrometry to separate and analyze mixtures of compounds. LC is used to resolve complex mixtures, while MS ionizes and analyzes individual resolved components based on their mass-to-charge ratio. Common interfaces like electrospray ionization are used to transfer samples from LC into the mass spectrometer without degrading thermally labile compounds. LC-MS has various applications including quantitative bioanalysis, clinical drug monitoring, pharmacokinetic studies, and impurity profiling.
This document provides an overview of the history and methods of microbial identification. It discusses how identification methods have evolved from using tubed and plated media in the 1960s to now using miniaturized biochemical reactions and system-dependent approaches comparing reaction patterns to databases. Modern rapid identification approaches include varying conventional testing, unique substrates that detect activity without growth, antigen-antibody reactions, and molecular detection methods. Specific techniques like colorimetry, fluorescence, and turbidity are used to detect metabolic activity. Rapid tests for identifying common bacteria like Staphylococcus aureus and Streptococcus pyogenes using agglutination, chromogenic media, DNA probes, PCR, and immunochromatographic assays are also overviewed.
This document provides an introduction to various spectroscopy techniques including absorption spectroscopy, emission spectroscopy, and mass spectrometry. It then discusses specific techniques like atomic absorption spectroscopy (AAS), flame emission spectroscopy (FES), and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The key components, working principles, and applications of these techniques are described. Sample preparation procedures including drying, grinding, and acid digestion are also outlined. Finally, quality control procedures like replicates, spikes, and standard reference materials are mentioned.
Brian D. Strahl's lecture presented in the BIOTECHNIQUES VIRTUAL SYMPOSIUM on "The Cell Landscape: From Genotype to Phenotype" Wednesday, October 3, 2012
Theories of staining (2) / dental implant courses by Indian dental academy Indian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.for more details please visit
www.indiandentalacademy.com
This document summarizes histology related products and procedures. It discusses fixation, decalcification, dehydration, clearing, infiltration, embedding, sectioning, staining, and mounting of tissue samples. It also briefly mentions liquid scintillation counting, which is used to detect radioactive samples by converting radiation into light flashes that are then counted.
Learn about Waters technologies for analyzing oligonucleotides with LC-MS. We offer solutions for both oligo characterization and QC monitoring. Learn more: http://www.waters.com/oligos
Analysis of racemic acid derivitives by chiral high performance liquid chroma...Tara K Orech
This document summarizes the analysis of racemic acid derivatives using chiral high performance liquid chromatography. It discusses the optimization of factors like wavelength, flow rate, and solvent composition to separate enantiomers of amino acids and hydroxyphosphonates. Initial results showed separation of valine enantiomers, with further research on tyrosine, phenylalanine, and complex hydroxyphosphonate derivatives planned. The goal is to optimize conditions to obtain 50:50 separation of enantiomer mixtures.
Ultrafiltration as pretreatment to reduce algae bloom in reverse osmosis plantsJorge J. Malfeito-Sánchez
Presentation at the IDA2013 Conference in Tianjin about the ultrafiltration as a pretreatment for reverse osmosis seawater desalination plants and their behaviour in case of algae bloom.
Isoelectric focusing is a technique that separates molecules like proteins based on their isoelectric point, which is the pH at which the molecule has no net charge. It was developed in the 1960s and allows for much better resolution than older techniques. The process involves creating an immobilized pH gradient using carrier ampholytes, loading protein samples, and applying an electric field to cause proteins to migrate to the point in the gradient matching their isoelectric point. The separated proteins can then be visualized through staining. Isoelectric focusing is useful for applications like identifying serum proteins and aiding in proteomics research.
Endodontic sealers and irrigating solutions /prosthodontic coursesIndian dental academy
The Indian Dental Academy is the Leader in continuing dental education , training dentists in all aspects of dentistry and
offering a wide range of dental certified courses in different formats.