Pertussis, or whooping cough, is an acute infectious disease caused by the bacterium Bordetella pertussis, characterized by severe coughing fits that may lead to respiratory distress, particularly in infants. It is highly communicable, primarily transmitted through respiratory droplets, and exhibits a clinical course divided into three stages: catarrhal, paroxysmal, and convalescent. Vaccination remains critical for prevention, with specific recommendations for various age groups and antibiotic treatment available for cases and close contacts.

1. Pertussis
Pertussis, or whooping cough, is an acute infectious disease caused by the
bacterium Bordetella pertussis. Outbreaks of pertussis were first described in the
16th century, and the organism was first isolated in 1906.
Pertussis remains a major health problem among children in developing
countries, In 2018, there were more than 151 000 cases of pertussis
globally(WHO).
B. pertussis is a small, aerobic gram-negative rod. It is fastidious and requires
special media for isolation .

2. Pertussis
Pathogenesis
Pertussis is primarily a toxin-mediated
disease. The bacteria attach to the cilia of
the respiratory epithelial cells, produce
toxins that paralyze the cilia, and cause
inflammation of the respiratory tract,
which interferes with the clearing of
pulmonary secretions. Pertussis antigens
appear to allow the organism to evade
host defenses in that lymphocytosis is
promoted but chemotaxis is impaired.

3. Pertussis
Clinical Features
The incubation period of pertussis is commonly 7–10 days, with a range of 4–
21 days. The clinical course of the illness is divided into three stages.
The first stage, the catarrhal stage, is characterized by the insidious onset of
coryza (runny nose), sneezing, low-grade fever, and a mild, occasional cough
similar to the common cold. The cough gradually becomes more severe and
after 1to 2 weeks, the second, or paroxysmal, stage, begins. Fever is generally
minimal throughout the course of the illness. It is during the paroxysmal stage
that the diagnosis of pertussis is usually suspected. Characteristically, the
patient might have bursts, or paroxysms, of numerous, rapid coughs,
apparently due to difficulty expelling thick mucus from the tracheobronchial
tree.

4. At the end of the paroxysm, a long inspiratory effort is usually accompanied by
a characteristic high-pitched whoop. During such an attack, the patient may
become cyanotic. Children and young infants, especially, might appear very ill
and distressed. Vomiting and exhaustion might follow the episode. The person
does not appear to be ill between attacks
Paroxysmal attacks generally occur more frequently at night, with an average
of 15 attacks per 24 hours. During the first 1 or 2 weeks of this paroxysmal
stage, the attacks might increase in frequency, remain at the same level for 2-3
weeks, and then gradually decrease. The paroxysmal stage usually lasts 1-6
weeks but may persist for up to 10 weeks. Infants younger than age 6 months
may not have the strength to have a whoop, but they can have paroxysms of
coughing. Additionally, the classic whoop might not be present in persons with
milder disease

5. Pertussis
Clinical Features-cont.
In the convalescent stage, recovery is gradual. The cough becomes less
paroxysmal and disappears in 2 to 3 weeks. However, paroxysms often recur
with subsequent respiratory infections for many months after the onset of
pertussis.
Adolescents, adults and children partially protected by the vaccine may
become infected with B. pertussis but may have milder disease than infants and
young children. Even though the disease may be milder in older persons, those
who are infected may transmit the disease to other susceptible persons,
including unimmunized or incompletely immunized infants. Older persons are
often found to have the first case in a household with
multiple pertussis cases, and are often the source of
infection for children.

6. Pertussis
Pertussis Complications in Children
● Secondary bacterial pneumonia – most common
● Neurologic complications – seizures, encephalopathy more common
among infants
● Otitis media
● Anorexia , Dehydration
● Pneumothorax
● Epistaxis
● Subdural hematomas
● Hernias
● Rectal Prolapse

7. Reservoir
Pertussis is a human disease. Adolescents and adults are an important reservoir
for B. pertussis and are often the source of infection for infants.
Transmission
Transmission most commonly occurs person-to-person through contact with
respiratory droplets, or by contact with airborne droplets of respiratory
secretions. Transmission occurs less frequently by contact with an infected
person’s freshly contaminated articles
The diagnosis of pertussis is based on a clinical history of signs and
symptoms, as well as a variety of laboratory tests (e.g., culture, polymerase
chain reaction [PCR], and serology).

8. Communicability
Pertussis is highly communicable, as evidenced by secondary attack rates of
80% among susceptible household contacts. Persons with pertussis are
infectious from the beginning of the catarrhal stage through the third week
after the onset of paroxysms or until 5 days after the start of effective
antimicrobial treatment.
Medical Management
The medical management of pertussis cases is primarily supportive, although
antibiotics are of some value. This therapy eradicates the organism from
secretions, thereby decreasing communicability and, if initiated early, may
modify the course of the illness. Recommended antibiotics are azithromycin,
clarithromycin, and erythromycin. Trimethoprim-sulfamethoxazole can also
be used.

9. Pertussis
Medical Management--
An antibiotic effective against pertussis should be administered to all close
contacts of persons with pertussis, regardless of age and vaccination status.
All close contacts younger than 7 years of age who have not completed the
four-dose primary series should complete the series with the minimal
intervals. Close contacts who are 4–6 years of age and who have not yet
received the second booster dose (usually the fifth dose of DTaP) should be
vaccinated. The administration of Tdap to persons who have been exposed to a
person with pertussis is not contraindicated, but the efficacy of postexposure
use of Tdap is unknown.

10. Whole-Cell Pertussis Vaccine
Based on controlled efficacy trials conducted in the 1940s and on subsequent
observational efficacy studies, a primary series of four doses of whole-cell DTP
vaccine was 70%–90% effective in preventing serious pertussis disease.
Protection decreased with time, resulting in little or no protection 5 to 10 years
following the last dose.
Local reactions such as redness, swelling, and pain at the injection site occurred
following up to half of doses of whole-cell DTP vaccines. Fever and other mild
systemic events were also common. Concerns about safety led to the
development of more purified (acellular) pertussis vaccines that are associated
with a lower frequency of adverse reactions

11. Pertussis vaccination 2020
Age Type of vaccine
2 Months Penta 1 (DwPT+Hib+HepB)
4Months Penta 2 (DwPT+Hib+HepB)
6 Months Penta 3 (DwPT+Hib+HepB)
18Months DwPT
4-6 years DwPT
Dose of Penta and DPT is 0.5 ml and injected Intramuscular

12. A Cellular Pertussis Vaccine
Pediatric acellular pertussis vaccines are combined with diphtheria and
tetanus toxoids as DTaP and are approved for children 6 weeks through 6
years of age (to age 7 years).
For above 10 years till age 64 years , Tdap vaccine available .

13. Acellular Pertussis Vaccine
Acellular pertussis vaccine was significantly more effective than whole-cell
DTP. Mild local and systemic adverse reactions and more serious adverse
reactions (such as high fever, persistent crying, and seizures) occurred less
frequently among infants vaccinated with acellular pertussis vaccines than
among those vaccinated with whole-cell DTP.
Immunity following pertussis is not permanent. Persons with a history of
pertussis should receive a single dose of Tdap if it is otherwise indicated.

14. Pertussis
Tdap Recommendations
A single dose of Tdap is recommended for
 adolescents 11 through 18 years of age
 adults 19 and older
 children 7-10 years of age who are not fully vaccinated against pertussis*
* “Not fully vaccinated” against pertussis is defined as having received fewer
than 4 doses of DTaP, or having received 4 doses of DTaP but the last dose was
prior to age 4 years.

15. Acellular Pertussis Vaccine
DTaP Contraindications
● Severe allergic reaction to vaccine component or
following a prior dose
● Encephalopathy not due to another identifiable
cause occurring within 7 days after vaccination

16. DTaP Precaution
• Moderate or severe acute illness
• Temperature (40.5°C) or higher within 48 hours with no other identifiable
cause
• Progressive or unstable neurological disorder
• Uncontrolled seizures
• History of Arthus-type hypersensitivity reactions after a previous dose of
diphtheria toxoid- or tetanus toxoid-containing vaccine

17. DTaP Adverse Reactions
 Local reactions (pain, redness, swelling) in 20%-40%
Temp of 38.5 in
℃ 3%-5% or higher
 Moderate or severe adverse reactions
Arthus-type reactions are rare
Local reactions more common following 4th and 5th doses

19. Pertussis vaccine forms
• DPT
• DaPT-Pediatric formulation 6weeks to 6 years
• Tdap-Adult and adolescent formulation
In case pertussis contraindication
• DT (Pediatric formulation)
• Td (Adult formulation)

20. Pneumococcal Disease
Streptococcus pneumoniae
• S. pneumoniae first isolated by Pasteur in 1881, Confused with other causes
of pneumonia until discovery of Gram stain in 1884
• Gram-positive bacteria , 100 known serotypes at 2020
• Polysaccharide capsule important virulence factor
• Type-specific antibody is protective

21. Pathogenesis
Pneumococci are common inhabitants of the respiratory tract and may be
isolated from the nasopharynx of 5% to 90% of healthy persons.
Rates of asymptomatic carriage vary with age, environment, and the presence
of upper respiratory infections.
Among school-age children, 20% to 60% may be colonized.
Only 5% to 10% of adults without children are colonized, although on
military installations, as many as 50% to 60% of service personnel may be
colonized.
The duration of carriage varies and is generally longer in children than adults.
The relationship of carriage to the development of natural immunity is poorly
understood

22. Pneumococcal Disease in Adults
● Pneumococcal pneumonia
■Most common clinical presentation
■ Incubation period 1 to 3 days
■ Symptoms: Fever, chills, pleuritic chest pain, cough, rusty sputum,
dyspnea, tachypnea, hypoxia, tachycardia, malaise, weakness
●Pneumococcal bacteremia
■Can lead to arthritis, meningitis, and endocarditis
■ 12% overall case fatality ratio
● Pneumococcal meningitis
■ Symptoms: Headache, lethargy, vomiting, irritability, fever, nuchal rigidity,
cranial nerve signs, seizures, coma
■ 14% case fatality ratio

23. Pneumococcal Disease in Children
●Pneumococcal pneumonia
■Accounts for 25% to 30% of invasive disease in children age 2 years or
younger
●Pneumococcal bacteremia
■Accounts for 40% of invasive disease in children age 2 years or younger
●Pneumococcal meningitis
■S. pneumoniae leading cause of bacterial meningitis among children
younger than age 5 years
●Pneumococci common cause of acute otitis media

25. Pneumococcal Polysaccharide vaccine
PPSV23 is composed of purified preparations of pneumococcal capsular
polysaccharide from 23 types of pneumococci. The serotypes are: 1, 2, 3, 4, 5,
6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B,17F, 18C, 19A, 19F, 20, 22F, 23F,
and 33F. PPSV23 is administered by either intramuscular or subcutaneous
injection. Each dose of PPSV23 contains phenol as a preservative. It contains
no adjuvant or antibiotic.
In children younger than age 2 years, antibody response to PPSV23 is
generally poor. Overall, the vaccine is 60% to 70% effective in preventing
invasive disease caused by serotypes included in the vaccine.
● Less effective in preventing pneumococcal pneumonia

26. Pneumococcal Polysaccharide vaccine
• The characteristics of the pneumococcal polysaccharide were similar to
other polysaccharide vaccines (e.g., Hib, meningococcal).
• The response to the vaccine was typical of a T-independent antigen, most
notably an age-dependent immune response, and poor immunogenicity in
children 2 years of age and younger.
• In addition, no boost in antibody titer was observed with repeated doses,
the Ab that was produced was relatively low-affinity IgM, and switching to
IgG production was minimal.

27. Pneumococcal Conjugated vaccine
The first pneumococcal conjugate vaccine (PCV7) was licensed in the United
States in 2000. It includes purified capsular polysaccharide of seven
serotypes of S. pneumoniae (4, 9V, 14, 19F, 23F, 18C, and 6B) conjugated to
a nontoxic variant of diphtheria toxin known as CRM197. In 2010 a 13-
valent pneumococcal conjugate vaccine (PCV13)(Prevenar 13) was licensed
in the United States. It contains the 7 serotypes of S pneumoniae as PCV7
plus serotypes 1, 3, 5, 6A, 7F and 19A which are also conjugated to
CRM197.
More than 90% effective against invasive disease caused by vaccine
serotypes in children

29. Pneumococcal Carriage
Pneumococci are common inhabitants of the respiratory tract and may be
isolated from the nasopharynx of 5% to 90% of healthy persons. Rates of
asymptomatic carriage vary with age, environment, and the presence of
upper respiratory infections
Studies comparing patterns of pneumococcal carriage before and after
PPSV23 vaccination have not shown clinically significant decreases in
carrier rates among vaccines.
There is evidence that PCV7 reduces nasopharyngeal carriage, among
children, of pneumococcal serotypes included in the vaccine.

30. PCV13- (Prevenar 13)
■ 3-dose primary series at age 2, 4, and 6 months
■ Booster at age 12 through 15 months
■ Minimum age for dose 1 is 6 weeks
■ Minimum interval for doses before age 1 year is 4 weeks and age 1 year
or older is 8 weeks
■ Unvaccinated children age 7 months or older require fewer doses
■ Shared clinical decision making for age 65 years or older
●PPSV23
■ 1 dose for all adults age 65 years or older
● Schedule for PCV13 and PPSV23 varies by medical condition

31. Pneumococcal Conjugate Vaccine
Adverse Events
● Local reactions
■ polysaccharide – 30%–50%
■ conjugate – 5%–49%
● Fever, myalgia
■ polysaccharide – <1%
■ conjugate – 24%–35%
● Febrile seizures
■ conjugate – 1.2–13.7/100,000
● Severe adverse reactions
■ polysaccharide – rare
■ conjugate – 8%

32. Hemophilus influenzae type b Vaccines
Haemophilus influenzae is a gram-negative coccobacillus. It is a cause of
bacterial infections that are often severe, particularly among infants.. The
organism was given the name Haemophilus by Winslow, et al. in 1920. It was
not until 1933 that Smith, et al. established that influenza was caused by a
virus and that H. influenzae was a cause of secondary infection.
It has Polysaccharide capsule , six different serotypes (a-f) of polysaccharide
capsule , 95% of invasive disease caused by type b .

33. Bacteremia
2%
Cellulitis
6%
Arthritis
8%
Osteomyelitis 2%
Pneumonia
15%
Epiglottitis
17%
Meningitis
50%
Invasive disease caused by H. influenzae type b can affect many organ
systems. The most common types of invasive disease are meningitis,
epiglottitis, pneumonia, arthritis, and cellulitis.
Accounted for approximately 50%-65% of cases of meningitis , Hearing
impairment or neurologic sequelae in 15%-30% . Case-fatality rate 3%-6%
despite appropriate antimicrobial therapy

34. Hemophilus influenzae type b Vaccines
Reservoir
Humans (asymptomatic carriers) are the only known reservoir. Hib does
not survive in the environment on inanimate surfaces.
Transmission
The primary mode of Hib transmission is presumably by respiratory droplet
spread, although firm evidence for this mechanism is lacking. Neonates can
acquire infection by aspiration of amniotic fluid or contact with genital
tract secretions during delivery.
Communicability
The contagious potential of invasive Hib disease is considered to be
limited. However, certain circumstances, particularly close contact with a
case-patient (e.g., household, child care, or institutional setting) can lead to
outbreaks or direct secondary transmission of the disease.

35. Laboratory Testing
A Gram stain of an infected body fluid may demonstrate small,gram-
negative coccobacilli suggestive of H. influenzae disease. CSF, blood,
pleural fluid, joint fluid, and middle ear aspirates should be cultured on
appropriate media. A positive culture for H. influenzae establishes the
diagnosis. Laboratory Testing A Gram stain of an infected body fluid may
demonstrate small, gram-negative coccobacilli suggestive of H. influenzae
disease. CSF, blood, pleural fluid, joint fluid, and middle ear aspirates
should be cultured on appropriate media. A positive culture for H.
influenzae establishes the diagnosis.

36. Hemophilus influenzae type b Vaccines
Haemophilus influenzae type b Polysaccharide Protein Conjugate Vaccines
Conjugation is the process of chemically bonding a polysaccharide
(a somewhat ineffective antigen) to a protein “carrier,” which is a more
effective antigen. This process changes the polysaccharide from a T-
independent to a T-dependent antigen and greatly improves immunogenicity,
particularly in young children. In addition, repeat doses of conjugate vaccines
elicit booster responses and allow maturation of class-specific immunity with
predominance of IgG antibody. The conjugates also cause carrier priming and
elicit antibody to “useful” carrier protein

37. Hemophilus influenzae type b Vaccines
Hib conjugate vaccines licensed for use in infants are highly immunogenic.
More than 95% of infants will develop protective antibody levels after a
primary series.
Number of
doses
Age at 1st
Dose
(months)
Primary
series
Booster
4 2-6 3 doses 12-15
3 7-11 2 doses 12-15
2 12-14 1 dose 2 months later
1 15-59 1 dose No need
Our National program
The Hib vaccine are given at ages 2,4,6 with new Penta vaccine (DwPT-Hep
B-Hib)

38. Meningococcal Meningitis
Neisseria meningitidis is a leading cause of bacterial meningitis and sepsis in
the United States. It can also cause focal disease, such as pneumonia and
arthritis. N. meningitidis is also a cause of epidemics of meningitis and
bacteremia in sub-Saharan Africa (meningitis belt). The WHO has estimated
that meningococcal disease was the cause of 171,000 deaths worldwide in
2000.
Aerobic gram-negative bacteria At least 13 serogroups based on characteristics
of the polysaccharide capsule Most invasive disease caused by serogroups A,
B, C, Y, and W-135 Relative importance of serogroups depends on geographic
location and other factors (e.g. age)

40. Clinical Features
Incubation period 3-4 days (range 2-10 days)
Abrupt onset of fever, meningeal symptoms, hypotension, and rash
Fatality rate 9%-12%; up to 40% in meningococcemia
Case classification
Suspected: a case that meets the clinical case definition
Probable: a suspected case as defined above, and turbid CSF (with or without
positive Gram stain), or ongoing epidemic and epidemiological link to a
Confirmed case.
Confirmed: a suspected or probable case with laboratory confirmation
(positive CSF antigen detection or positive culture)

41. Meningococcal Meningitis
Meningitis
47.3%
Bacteremia
43.3%
Pneumonia
6.0%
Arthritis
2.0%
Otitis media
1.0%
Epiglottitis
0.3%

43. Meningococcal Meningitis
Reservoir :
Humans are the only natural reservoir of meningococcus. As many as 10% of
adolescents and adults are asymptomatic transient carriers of N. meningitidis,
most strains of which are not pathogenic (i.e., strains that are not groupable).
Transmission :Primary mode is by respiratory droplet spread or by direct
contact.
Temporal Pattern :Meningococcal disease occurs throughout the year,
however, the incidence is highest in the late winter and early spring.
Communicability : The contagious potential of invasive Hib disease is
considered to be limited. However, certain circumstances, particularly close
contact with a case-patient (e.g., household, child care, or institutional setting)
can lead to outbreaks or direct, secondary transmission of the disease

44. Meningococcal Polysaccharide Vaccine (MPSV4)
The current quadrivalent composed of four purified bacterial capsular
polysaccharides A, C, W,Y . The vaccine contains lactose as a stabilizer.
MPSV4 is administered by subcutaneous injection. The vaccine is available in
single-dose and 10-dose vials. Diluent for the single-dose vial is sterile water
without preservative. Diluent for the 10-dose vial is sterile water with
thimerosal added as a preservative. After reconstitution, the vaccine is a clear
colorless liquid.
The characteristics of MPSV4 are similar to other polysaccharide vaccines
(e.g., pneumococcal polysaccharide). The vaccine is generally not effective
in children younger than 2 years of age. In addition, little boost in antibody
titer occurs with repeated doses; the antibody which is produced is relatively
low-affinity IgM, and “switching” from IgM to IgG production is poor.

45. Meningococcal Conjugate Vaccines MenACWY
Meningococcal A, C, W, and Y polysaccharides conjugated to diphtheria
toxoid protein carrier , and it is approved for use in persons 9 months through
55 years of age. It is administered by intramuscular injection.
An advantage of conjugate vaccines is their ability to elicit immunologic
memory. Meningococcal conjugate vaccines prime the immune system, and
immunologic memory persists even in the absence of detectible bactericidal
antibodies.
Routine MenACWY Conjugated Vaccination Recommendations
 Administer at age 11 or 12 years with a booster dose at 16 years of age
 Healthy persons who receive their first dose of meningococcal conjugate vaccine at or
after age 16 years do not need a booster dose
 Routine vaccination not recommended after age 21 years for healthy persons who are not
at increased risk of exposure

46. Additional High-risk Groups
Meningococcal vaccination is recommended for persons at increased risk
for meningococcal disease Includes:
microbiologists who are routinely exposed to isolates of N. meningitidis
military recruits
Pilgrimages
Persons who travel to countries in which N. meningitides is hyperendemic
or epidemic, particularly areas in the Sub-Saharan African “meningitis belt”
Revaccinate every 5 years as long as the person remains at increased risk
Both MenACWY and MPSV4 are recommended for use in control of
meningococcal outbreaks caused by vaccine- preventable serogroups (A, C,
W, Y).

47. Meningococcal Meningitis
MenACWY-D Adverse Events
• Fever most frequently reported (16.8%)
• Headache (16.0%); injection- site erythema (14.6%); dizziness
(13.4%)
• Syncope ■ reported in 10%
• Serious adverse events rare

48. Methods of control of meningococcal meningitis outbreak
1. Vaccination: to prevent secondary cases around a sporadic case of meningococcal disease
2.Chemoprophylaxis: the aim of chemoprophylaxis is to prevent secondary cases by
eliminating nasopharyngeal carriage. To be effective in preventing secondary cases,
chemoprophylaxis must be initiated as soon as possible (i.e. not later than 48 hours after
diagnosis of the case). Its use should be restricted to close contacts of a case, which are
defined as:
 Household members (i.e. persons sleeping in the same dwelling as the case)
 Institutional contacts who shared sleeping quarters (i.e. for boarding-school pupils,
roommates; for military camps, persons sharing a barracks);
 Nursery school or childcare center contacts (i.e. children and teachers who share a
classroom with the case);
The drugs recommended by the Iraqi Ministry of Health are Rifampin (children and adults) or
Ciprofloxacin (adults only).