This study examined the role of sex hormones in the progression of diabetes and renal disease in female T2DN rats. The rats were divided into 6 groups: males, females, ovariectomized females, females given dihydrotestosterone, etc. Protein excretion and glucose tolerance tests were performed. The results showed no significant differences between males and females or between hormone-altered female groups in glucose tolerance or plasma glucose and protein excretion levels. This suggests that sex hormones are not responsible for differences seen between male and female T2DN rats in blood glucose and protein excretion.