academic project

1. FIRST REVIEW
OF
PROJECT
PRESENTATION

2. NAME: KALAIVANI B
REG NO:212420214011
DEPARTMENT: BIOTECHNOLOGY
YEAR: FINAL YEAR
COLLEGE: SREE SASTHA INSTITUTE OF
ENGINEERING AND TECHNOLOGY

3. EXTERNAL GUIDE
Dr.P.GANESAN,
SENIOR SCIENTIST,
DEPARTMENTOF BIOTECHNOLOGY,
CSIR-CLRI.
INTERNAL GUIDE
Ms.JENNIFIER,
ASSISTANT PROFESSOR,
DEPARTMENTOF BIOTECHNOLOGY,
SREE SASTHA INSTITUTE OF
ENGINEERING AND TECHNOLOGY.

4. TITLE
EXTRACTION, PURIFICATION AND
CHARACTERISTICS OF PHYCOERYTHRIN FROM
GRACILLARIA

5. AIM:
To extract and purify Phycoerythrin from Gracillaria.
OBJECTIVE:
✓ To isolate Phycoerythrin pigment protein complex from Gracillaria.
✓ To extract and purify the Phycoerythrin complex which is been isolated.
✓ To study the various characteristics of Phycoerythrin.

6. INTRODUCTION
✓ Light harvesting protein pigmented complex from red algae and cryptophytes.
✓ Any of a group of red photosynthetic pigments present in red algae and some
cyanobacteria.
✓ Used as a fluorescent probe or as a colorant in the food and cosmetic industries.
✓Substantial economic impact due to its potential as a natural food colorant.

7. R-PE:
•Molecularweight:
✓ 240 to 260 Kda.
✓most abundantin Rhodophytes.
✓ composed of an (αβ)6 γ complex.
•Subunits:
✓ α- 2 phycoerythrobilins(PEB)
✓ β- 2 or 3 PEBs and 1 phycourobilin(PUB)
✓ γ(s)
• Stability:
✓ Good stability towardstemperature, upto 40°C.
✓ At 60°C,the reduction in concentrationof B- Phycoerythrin and R- Phycoerythrin. 50+ 3.4% and
70+ 0.18% respectively.
• Charge
✓ negatively charge.
• pI
✓ 4.89 where pH 4.5 to 6.0net charge will become zero.

8. Gracillaria Algae Structure of Phycoerythrin

9. Gracillaria Algae → Wash and freeze at 4°C to 10°C. → Lyophilization process → Grain the algae in the form of powder.
↓
Measuring the weight.
↓
Take upto 5g of grained sample.
↓
Add 0.1 molar of sodium phosphate buffer.
|
Purification. ← Collect the supernatant ← Ultra sonification ← Maceration for 30mins ↲
↓
HPLC
↓
Physio - Chemical Characteristics.
METHODOLOGY

10. APPLICATION