This experiment characterized the microenvironment of a gel mixture composed of 50% kappa-carrageenan and 50% type B gelatin at various phases and temperatures using the luminescence probe Ru(bpy)3 2+. Emission spectra and fluorescence decay were measured at 10, 23.5, 30, 37.5, 50, and 60°C to observe changes in maximum emission wavelength and quenching rate constant. The Arrhenius plot showed that the Ru(bpy)3 2+ decay rate increased with temperature. The maximum emission wavelength remained constant within phases but increased slightly between phases, indicating a small decrease in energy emission through phase changes.