This document provides an overview of molecular biology concepts including:
- DNA contains genetic codes that are identical in all cells of an organism. The genome is the entire DNA complement and controls protein production.
- DNA is made up of nucleotides that form base pairs (A-T, C-G) along a phosphate-sugar backbone forming two complementary strands that twist into the double helix structure.
- Genes are portions of DNA that are transcribed into messenger RNA (mRNA), which is then translated into proteins according to its nucleotide sequence using codons.
Covers the flow of information from DNA to Protein synthesis, Transcription, Types of RNA, Genetic code, Protein Synthesis, Cell Function and cell reproduction
Covers the flow of information from DNA to Protein synthesis, Transcription, Types of RNA, Genetic code, Protein Synthesis, Cell Function and cell reproduction
Gene regulation, History and Evolution , Traditional Methods:
Northern blot
quantitative reverse transcription PCR (qRTPCR)
serial analysis of gene expression(SAGE) and
DNA microarrays.
DNA Chip
These are simplified slides from the second lecture in a three-lecture series by Dr. Sidra Arshad, diving into the significance of genetic control which stands as one of the most intricate, yet complex, physiological control mechanisms intimately interlinked with homeostasis and cellular functioning.
Learning objectives:
1. Briefly describe the process of translation
2. Discuss the mechanisms of genetic control of cell functions
3. Describe the cell cycle
4. Briefly describe the process of DNA replication
Study Resources:
1. Chapter 3, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 1, Ganong’s Review of Medical Physiology, 26th edition
3. DNA replication, https://www.genome.gov/genetics-glossary/DNA-Replication
Black head histomoniasis in poultry characterised by ulceration of ceca and filling of ceca with caseouse material and cyanosis of head in turkey, drop in egg production in chicken, heterakis gallinarum worm act as a vector for transport of histomonas parasite to chicken body
Gene regulation, History and Evolution , Traditional Methods:
Northern blot
quantitative reverse transcription PCR (qRTPCR)
serial analysis of gene expression(SAGE) and
DNA microarrays.
DNA Chip
These are simplified slides from the second lecture in a three-lecture series by Dr. Sidra Arshad, diving into the significance of genetic control which stands as one of the most intricate, yet complex, physiological control mechanisms intimately interlinked with homeostasis and cellular functioning.
Learning objectives:
1. Briefly describe the process of translation
2. Discuss the mechanisms of genetic control of cell functions
3. Describe the cell cycle
4. Briefly describe the process of DNA replication
Study Resources:
1. Chapter 3, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 1, Ganong’s Review of Medical Physiology, 26th edition
3. DNA replication, https://www.genome.gov/genetics-glossary/DNA-Replication
Black head histomoniasis in poultry characterised by ulceration of ceca and filling of ceca with caseouse material and cyanosis of head in turkey, drop in egg production in chicken, heterakis gallinarum worm act as a vector for transport of histomonas parasite to chicken body
New Drug Discovery and Development .....NEHA GUPTA
The "New Drug Discovery and Development" process involves the identification, design, testing, and manufacturing of novel pharmaceutical compounds with the aim of introducing new and improved treatments for various medical conditions. This comprehensive endeavor encompasses various stages, including target identification, preclinical studies, clinical trials, regulatory approval, and post-market surveillance. It involves multidisciplinary collaboration among scientists, researchers, clinicians, regulatory experts, and pharmaceutical companies to bring innovative therapies to market and address unmet medical needs.
- Video recording of this lecture in English language: https://youtu.be/lK81BzxMqdo
- Video recording of this lecture in Arabic language: https://youtu.be/Ve4P0COk9OI
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
The prostate is an exocrine gland of the male mammalian reproductive system
It is a walnut-sized gland that forms part of the male reproductive system and is located in front of the rectum and just below the urinary bladder
Function is to store and secrete a clear, slightly alkaline fluid that constitutes 10-30% of the volume of the seminal fluid that along with the spermatozoa, constitutes semen
A healthy human prostate measures (4cm-vertical, by 3cm-horizontal, 2cm ant-post ).
It surrounds the urethra just below the urinary bladder. It has anterior, median, posterior and two lateral lobes
It’s work is regulated by androgens which are responsible for male sex characteristics
Generalised disease of the prostate due to hormonal derangement which leads to non malignant enlargement of the gland (increase in the number of epithelial cells and stromal tissue)to cause compression of the urethra leading to symptoms (LUTS
Pulmonary Thromboembolism - etilogy, types, medical- Surgical and nursing man...VarunMahajani
Disruption of blood supply to lung alveoli due to blockage of one or more pulmonary blood vessels is called as Pulmonary thromboembolism. In this presentation we will discuss its causes, types and its management in depth.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Acute scrotum is a general term referring to an emergency condition affecting the contents or the wall of the scrotum.
There are a number of conditions that present acutely, predominantly with pain and/or swelling
A careful and detailed history and examination, and in some cases, investigations allow differentiation between these diagnoses. A prompt diagnosis is essential as the patient may require urgent surgical intervention
Testicular torsion refers to twisting of the spermatic cord, causing ischaemia of the testicle.
Testicular torsion results from inadequate fixation of the testis to the tunica vaginalis producing ischemia from reduced arterial inflow and venous outflow obstruction.
The prevalence of testicular torsion in adult patients hospitalized with acute scrotal pain is approximately 25 to 50 percent
Surat @ℂall @Girls ꧁❤8527049040❤꧂@ℂall @Girls Service Vip Top Model Safe
intro-molecular-biology.ppt
1. Introduction to Molecular Biology
(based on pages 25-36 of Kohane et al’s book)
Chitta Baral
Arizona State University
2. Cells, genome, gene and DNA
• Almost all cells of a living organism contain an identical
set of codes describing the genes and their regulation
• This code is encoded as one or more strands of DNA
• Cells from the different parts of an organism have the
same DNA
– Distinction: The portion of the DNA that is transcribed and
translated into protein
• Genome: entire complement of DNA molecules of each
organism
• Overall function of genome: Control the generation of
molecules (mostly proteins) that will
– Regulate the metabolism of a cell and its response to the
environment, and
– Provide structural integrity.
3. Structure of DNA
• Made up of 4 different building blocks (so
called nucleotide bases), each an almost
planar nitrogenic organic compound
– Adenine (A)
– Thymine (T)
– Guanine (G)
– Cytosine (C)
– Base pairs (A -- T, C -- G)
4. Structure of DNA -- 2
• Base pairs (A -- T,C -- G) are attached to a sugar phosphate
backbone to form one of 2 strands of a DNA molecule.
– Phosphate ((PO4) -3)
– Deoxyribose
• Two strands are bonded together by the base pairs (A – T, C – G).
• Results in mirror image or complementary strands, each is twisted
(or helical), and when bonded they form a double helix.
• Direction of each strand (5’ meaning beginning or 3’ meaning end of
the strand)
– 5’ and 3’ refer to position of bases in relation to the sugar molecule in
the DNA backbone.
– Are important reference points to navigate the genome.
– 2 complementary strands are oriented in opposite direction to each
other.
6. Duplication of DNA
• Occurs through the coordinated action of
many molecules, including
– DNA polymerases (synthesizing new DNA),
– DNA gyrases (unwinding the molecule), and
– DNA ligases (concatenating segments
together)
7. Transcription of DNA to RNA
• Why transcription:
– (For genome) to direct or effect changes in the cytoplasm of the
cell
– Need to generate new proteins to populate the cytosol
(heteregenous intracellular soup of the cytoplasm)
• Note: DNA is in the nucleus, while proteins are needed in
the cytoplasm, where many of the cell’s functions are
performed.
• Coding region of the DNA is copied to a more transient
molecule called RNA
– Gene is a single segment of the coding region that is transcribed
into RNA
– Generation of RNA from DNA (in the nucleus) is done trough a
process called transcription
8. Transcription
• RNA (Ribonucleic acid)
– Similar to DNA (except for a chemical modification of the sugar backbone)
– Instead of T contains U (Uracil) which binds with A.
– Is not double stranded but single stranded
– RNA molecules tend to fold back on themselves to make helical twisted and rigid
segments.
• RNA is synthesized
– By unwinding the DNA double helix separating the 2 strands.
– Using one of the strands as a template along which to build the RNA molecule
– Accomplished by Enzyme RNA polymerase (binds to promoter and copies or
transcribes the gene in its full length)
– Resulting molecule is called Pre-mRNA
– Single stranded pre-mRNA is then processed.
– Splicing (mediated by spliceosome consisting of RNA and proteins) removes the
introns.
– Ends modified (Capping modifies 5’ end and Polyadenylation adds adenines at
the 3’ end) to enhance stability
9. mRNA, ORFs, etc.
• Each cell has 20 to 30 pg of RNA (1% of the cell mass)
• The RNA that codes for proteins is called messenger
RNA (mRNA)
• The part of DNA that provides that code is called Open
Reading Frame (ORF)
• When read in the standard 5’ to 3’ direction, the portion
of DNA before the ORF is considered upstream and the
portion following the ORF is considered downstream.
• Promoter regions: DNA sequence upstream of an ORF
– Specifically determine which gene to transcribe
– Transcription factors: proteins that contain part that bind to
specific promoter regions, thus activating or deactivating
transcription of the downstream ORF
10. Coding and non-coding RNA
• Not all RNA code for proteins
– 4% of total RNA is made of coding RNA
– Of the non-coding RNA
• Ribosonal RNA (rRNA) and transfer RNA(tRNA) are used in
the various protein translational apparatus
• Small nuclear RNA (snRNA) – found in eucaryotes, is part of
the splicing apparatus
• Small nucleolar RNA (snoRNA) involved in methylation of
rRNA
• Small cytoplasmic RNA (scRNA) plays a role in the
expression of specific genes
11. Prokaryotic and Eukaryotic cells
• Eukaryotes: Organisms whose cells contain
compartments or organelles within the cell, such
as mitochondria and nucleus
– Animals, plants
• Prokaryotes: Whose cells do not have these
organelles (e.g. bacteria)
– Most prokaryotes have a smaller genome, typically
contained in a single circular DNA molecule.
– Additional genetic information may be contained in
smaller satellite pieces of DNA called plasmids
12. More on transcription
• Most eukaryotic genes have exons (portions that will be
put in the mRNA) and introns (that are normally spliced
out)
– Some introns may have a promoter-like control of the
transcription process
– If an intron is not spliced out then an alternative splicing product
is created.
– Various tissue types can flexibly alter their gene products
through alternative splicing
• Post-splicing (in Eukaryotes)
– The generated mRNA is exported (through nuclear pore
complexes) to the cytoplasm
– In the cytoplasm, the ribosonal complex (containing hundreds of
proteins and special function RNA molecules) acts to generate
the protein on the basis of the mRNA code.
13. Translation
• Process of generating a protein or polypeptide from an
mRNA molecule is known as translation.
• Protein: a polymer or chain of aminoacids, whose
sequence is determined by the mRNA template
– 3 nulceotides code for 20 naturally occurring amino acids
– 43 = 64; thus several trinucleotide sequences (codons)
correspond to a single amino acid.
– There is no nucleotide between codons, and a few codons
represent start and stop.
– Notable exceptions: code of naturally occurring selenocysteine is
identical to that for a stop codon, except for a particular
nucleotide sequence further downstream.
14. Translocation of proteins
• A newly formed protein need to be translocated
to the right place to perform its function (such as
structural protein in the cytoskeleton, as a cell
membrane receptor, as a hormone that is to be
secreted by the cell, etc.)
• Signal peptide (header): part of the polypeptide
that is one of the determinant of its location and
handling
15. Transcriptional programs
• Initiation of the transcription process can be caused by
external events or by a programmed event within the
cell.
• External events
– Piezoelectric forces generated in bones through walking can
gradually stimulate osteoblastic and osteoclastic transcriptional
activity to cause bone remodelling; Heat shock
– Appearance or disappearance of new micro or macronutrients
around the cell; binding of distantly secreted hormones
• Internally programmed sequences of transcriptional
expression (eg. clock and per genes)
• Pathological internal derangements of the cell
– Self-repair or damage detection programs can trigger apoptosis
(self-destruction) under conditions such as irreparable DNA
damage
16. Biological function of proteins
• Enzyme catalysis: DNA polymerases, lactate dehydrogenase,
trypsin
• Transport: hemoglobin, membrane transporters, serum albumin
• Storage: ovalbumin, egg-white protein, ferritin
• Motion: myosin, actin, tubulin, flagellar proteins
• Structural and mechanical support: collagen, elastin, keratin, viral
coat proteins
• Defense: antibodies, complement factors, blood clotting factors,
protease inhibitors
• Signal transduction: receptors, ion channels, rhodopsin, G
proteins, signalling cascade proteins
• Control of growth, differentiation and metabolism: repressor
proteins, growth factors, cytokines, bone morphogenic proteins,
peptide hormones, cell adhesion proteins
• Toxins: snake venoms, cholera toxin
17. Gene expression studies
• Allow you to understand how a gene is regulated in a tissue or a cell type.
• Most useful way of studying gene expression is by measuring the levels of
mRNA produced from a particular gene in a particular tissue.
• Application: to understand certain biological process it is useful to study the
differences in gene expression which occur during such processes. E.g.
– It is of interest to know which genes are induced or repressed, say in the liver,
after a particular drug is taken.
– Or which genes are expressed in a tumor but not in the surrounding normal
tissue.
• Some techniques for analyzing mRNA level of a single gene or to quantify
gene expression
– Northern blots
– Quantitative reverse transcriptase PCR (QT-RT-PCR)
– DNA microarrays
– Proteomics (analysis of the protein synthesis that results from gene expression)
18. DNA microarrays
• Consist of thousands of DNA probes corresponding to different genes
arranged as an array.
• Each probe (sometimes consisting of a short sequences of synthetic DNA)
is complementary to a different mRNA (or cDNA)
• mRNA isolated from a tissue or cell type is converted to fluoroscently
labeled mRNA or cDNA and is used to hybridize the array.
• All expressed genes in the sample will bind to one probe of the array and
generate a fluoroscent signal.
• A DNA microarray can interrogate the level of transcription of several
thousand of different genes from one sample in one experiment. (One DNA
microarray experiment reveals the mRNA levels of 1000s of genes from one
tissue or cell type at one time point)
• Particularly useful when studying the effect of environmental factors on
gene expression.
• A fingernail size chip can interrogate 10,000 different transcripts. Chip has
30-40 different probes; half of them are designed to perfectly match 20
nucleotide stretches of the gene and the other half contains a mismatch as
a control to test for specificity of the hybridization signal.
19. SNPs
(single nucleotide polymorphisms)
• Genetic basis for organismal diversity is due in large part
to differences in sequences, also known as
polymorphisms of each gene.
• Most of these polymorphisms differ from one another by
one nucleotide and are known as SNPs.
• Due to the small portion of the genome coding for
proteins and the redundancy in the mRNA code, only
some SNPs will result in differently constructed proteins.
• It is believed that genomic markers such as SNPs
spaced every 1000 bases will be sufficient to
unambiguously resolve the span of genome associated
with a phenotypic difference to a single gene.
20. Gene clustering dogma
• Genes that appeared to be expressed in
similar patterns are mechanistically
related.
• I.e., if we can find genes whose
expression patterns approximate one
another we can possibly conclude that
they have functions that are related.