This document provides an overview of intracytoplasmic sperm injection (ICSI). ICSI involves injecting a single sperm directly into a mature egg under a microscope, which differs from conventional in vitro fertilization where fertilization occurs outside the body. ICSI may be recommended for couples experiencing issues like low sperm counts, abnormal sperm, or problems with antibodies. The process involves sperm and egg retrieval followed by cleaning and injecting a sperm into an egg's cytoplasm. Success rates vary depending on patient factors, though ICSI enables fertilization when few sperm are available. Potential risks include genetic issues being passed to offspring if the father's sperm is abnormal.
Study on intra cytoplasmic sperm injection (icsiDr. sreeremya S
Intra cytoplasmic sperm injection(ICSI) is a recently developing technique in the field of developmental biology. The rationale behind artificial insemination is increasing the gamete density at the site of fertilization.
The Assisted Reproductive Techniques - ART (IVF, IUI, ZIFT, GIFT, ICSI etc.)Muhammad Anas Shamsi
Assisted reproductive technology (ART) is used to treat infertility. It includes fertility treatments that handle both a woman's egg and a man's sperm. It works by removing eggs from a woman's body. The eggs are then mixed with sperm to make embryos. The embryos are then put back in the woman's body. In vitro fertilization (IVF) is the most common and effective type of ART.
Study on intra cytoplasmic sperm injection (icsiDr. sreeremya S
Intra cytoplasmic sperm injection(ICSI) is a recently developing technique in the field of developmental biology. The rationale behind artificial insemination is increasing the gamete density at the site of fertilization.
The Assisted Reproductive Techniques - ART (IVF, IUI, ZIFT, GIFT, ICSI etc.)Muhammad Anas Shamsi
Assisted reproductive technology (ART) is used to treat infertility. It includes fertility treatments that handle both a woman's egg and a man's sperm. It works by removing eggs from a woman's body. The eggs are then mixed with sperm to make embryos. The embryos are then put back in the woman's body. In vitro fertilization (IVF) is the most common and effective type of ART.
INTRACYTOPLASMIC MORPHOLOGICALLY SELECTED SPERM INJECTION is a technique used in IVF treatment to examine and select sperm using a high-magnification digital imaging microscope for microinjection into the egg.
ART ( ASSISTED REPRODUCTIVE TECHNOLOGIES).THIS THE ARTIFICIAL REPRODUCTION TE...Anand P P
Artificial reproductive technology helps to produce the offspring without direct biological mating.several methods are used to artificial reproduction like zift , gift ,INTRA CYTOPLASMIC SPERM INGECTION
Embryo transfer refers to a step in the process of assisted reproduction in which embryos are placed into the uterus of a female with the intent to establish a pregnancy.
This presentation consist brief introduction about the IVF (In-vitro fertilization) in humans.
There are more than 15 slides which gives you basic study about the history of IVF, causes of IVF, basic steps involved in IVF process, ethical issues and etc.
Hope it will help you and make you easy to understand the IVF.
process of preserving an embryo at sub-zero temperatures, generally at an embryogenesis stage corresponding to pre-implantation.this presentation will clear you about slow freezing rapid freezing and vitrification method of embryo freezing.
Embryo culture is a component of in vitro fertilisation where in resultant embryos are allowed to grow for some time in an artificial medium before being inserted into the uterus.
INTRACYTOPLASMIC MORPHOLOGICALLY SELECTED SPERM INJECTION is a technique used in IVF treatment to examine and select sperm using a high-magnification digital imaging microscope for microinjection into the egg.
ART ( ASSISTED REPRODUCTIVE TECHNOLOGIES).THIS THE ARTIFICIAL REPRODUCTION TE...Anand P P
Artificial reproductive technology helps to produce the offspring without direct biological mating.several methods are used to artificial reproduction like zift , gift ,INTRA CYTOPLASMIC SPERM INGECTION
Embryo transfer refers to a step in the process of assisted reproduction in which embryos are placed into the uterus of a female with the intent to establish a pregnancy.
This presentation consist brief introduction about the IVF (In-vitro fertilization) in humans.
There are more than 15 slides which gives you basic study about the history of IVF, causes of IVF, basic steps involved in IVF process, ethical issues and etc.
Hope it will help you and make you easy to understand the IVF.
process of preserving an embryo at sub-zero temperatures, generally at an embryogenesis stage corresponding to pre-implantation.this presentation will clear you about slow freezing rapid freezing and vitrification method of embryo freezing.
Embryo culture is a component of in vitro fertilisation where in resultant embryos are allowed to grow for some time in an artificial medium before being inserted into the uterus.
Intracytoplasmic sperm injection (ICSI) is a segment of IVF treatment and focused on infertility caused by malefactors. Infertility issues can be found in either male partners or female partners.
The expansion of ICSI is that intracytoplasmic sperm injection. The process imperatively works as the semen is brought back from the male, the best sperm selected from it, and then artificially injected into the egg for fertilization.
Since the birth of Louise Brown, the first baby born through in vitro fertilization, in 1978, assisted reproductive technology has advanced significantly. The success rate of in vitro fertilization (IVF) has risen dramatically over the last four decades as a result of developments in incubation methods, micromanipulation technology, and a general understanding of assisted reproduction.
ICSI is a type of IVF in which a single live sperm is injected directly into the human egg. It is a blessing for couples with infertility, especially those with male factor infertility. Check out this video to know more about ICSI...‼️
Best IVF Centre in Jalandhar | IVF Cost in Punjabseoservicesit
Janam Fertility Centre provides the best treatments for infertility at affordable cost. Our Best IVF Centre in Jalandhar also Offer Best IVF Cost in Punjab. Get started on your fertility journey. Your parenthood dream can come true. Consult with an IVF expert at Janam Fertility Centre.
In vitro fertilization (IVF) is the process of fertilizing an egg with sperm outside the body in a
laboratory setting and then implanting and developing the embryo in the woman's uterus, where it
will implant and grow into a baby.
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Ozempic: Preoperative Management of Patients on GLP-1 Receptor Agonists Saeid Safari
Preoperative Management of Patients on GLP-1 Receptor Agonists like Ozempic and Semiglutide
ASA GUIDELINE
NYSORA Guideline
2 Case Reports of Gastric Ultrasound
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
These simplified slides by Dr. Sidra Arshad present an overview of the non-respiratory functions of the respiratory tract.
Learning objectives:
1. Enlist the non-respiratory functions of the respiratory tract
2. Briefly explain how these functions are carried out
3. Discuss the significance of dead space
4. Differentiate between minute ventilation and alveolar ventilation
5. Describe the cough and sneeze reflexes
Study Resources:
1. Chapter 39, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 34, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 17, Human Physiology by Lauralee Sherwood, 9th edition
4. Non-respiratory functions of the lungs https://academic.oup.com/bjaed/article/13/3/98/278874
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
ARTIFICIAL INTELLIGENCE IN HEALTHCARE.pdfAnujkumaranit
Artificial intelligence (AI) refers to the simulation of human intelligence processes by machines, especially computer systems. It encompasses tasks such as learning, reasoning, problem-solving, perception, and language understanding. AI technologies are revolutionizing various fields, from healthcare to finance, by enabling machines to perform tasks that typically require human intelligence.
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
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New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
3. Key words
• (ICSI) Intracytoplasmic sperm injection
• (IVF) in vitro fertilization
3
4. contents
Introduction
Who might need ICSI.
How is ICSI performed/stages of ICSI.
couple Counselling.
Benefits of ICSI.
Fertilization and pregnancy success rates with ICSI
Potential problems with ICSI.
Alternative treatment options to IVF with ICSI.
References .
4
5. what is intracytoplasmic sperm injection (ICSI).
Intracytoplasmic sperm injection is a micromanipulation
technique used in the process of fertilization (IVF). It
involves injecting a single sperm in to the centre of a
mature oocyte under a microscope (i.e. injecting sperm
through the egg’s shell so that the sperm does not need
to penetrate the shell in order to fertilize the egg).
5
6. (ICSI) differs from conventional (IVF).
Intracytoplasmic sperm injection (ICSI)
differs from conventional in vitro
fertilization (IVF) in that the embryologist
selects a single sperm to be injected
directly into an egg, instead of
fertilization taking place in a dish where
many sperm are placed near an egg.
6
(IVF)
(ICSI)
7. Who might need intracytoplasmic sperm injection?
• Couples experiencing the following infertility issues
may benefit from intracytoplasmic sperm injection:
I. Low numbers of sperm produced or ejaculated.
II. Sperm that are abnormal.
III. Problems with antibodies against sperm.
IV. Testicular cancer.
V. you are using frozen sperm in your treatment which is
not of optimum quality
7
8. Low numbers of sperm produced or ejaculated
o this can be due to retrograde ejaculation or a
blockage in the vas deferens, the tubes that carry
sperm out of the testes. A complete absence of sperm
in the ejaculate can be overcome if there is sperm
production inside the testes. Sperm retrieval
methods are used to extract sperm surgically to allow
intracytoplasmic sperm injection to take place.
8
9. Sperm that are abnormal.
o Sperm that are abnormal and cannot swim well. Obtaining
plenty of sperm in normal ejaculate is straightforward and
intracytoplasmic sperm injection can overcome the
problems with getting these immobile sperm to meet up
with the woman’s eggs.
Problems with antibodies against sperm.
o these can be produced by the male partner in the fluid of
the ejaculate, or by the female partner in cervical secretions.
9
10. Testicular cancer
occur in younger men and treatment may mean
that sperm production is affected. Sperm can
be frozen before cancer treatment (which has
a high success rate) and then the sperm can
be used for icsi.
10
11. How is ICSI performed/stages of ICSI …
I. couple Counselling
II. Collection of sperm
III. Collection of egg
IV. Cleaning and dilution
V. Intracytoplasmic sperm injection
VI. Culturing of injected cells(16 to 20 hours)
VII. Selection or separating of fertilized eggs
VIII.Transferring to fresh medium (2 to 5 days)
IX. (size 8 cells or more)
X. implantation or Cryopreservation.
11
12. couple Counselling
o All couples wishing to undergo (ICSI) should be counselled to the
commencement of treatment. Counselling should address any concerns the
couple may have also provide evidence based information regarding the
likely outcomes and what to expect while undergoing intracytoplasmic
sperm injection.
o Couples should also be aware that (ICSI) may fail, and be encouraged to
discuss alternative options (e.g. use of donor sperm, adoption). In addition,
couples undergoing (ICSI) should be aware of and discuss the increased risk
of congenital abnormalities, particularly in male offspring.
12
13. Sperm collection
o fresh ejaculate but this is not possible in all male
partners contributing sperm for intracytoplasmic
sperm injection
o Men who cannot ejaculate because of nerve
injury may be able to do so with electro-
ejaculation.
13
14. course of hormone treatment /Egg collection.
o first given a course of hormone treatment to stimulate
her ovaries to produce several eggs.
o Monitoring of woman
o These are monitored using ultrasound
Collection of eggs
once mature egg follicles have formed, the eggs are
collected using a thin tube passed through the vagina.
The eggs are then placed in special culture fluid in the
laboratory
14
15. Sperm washing is a form of sperm preparation
that is required is for IVF, because it removes
chemicals from the semen, which may cause
adverse reactions in the uterus. During the
sperm washing process sperm is separated
from the seminal fluid. This procedure
enhances the fertilizing capacity of the
sperm.
Cleaning and dilution
15
16. Intracytoplasmic sperm injection
o The mature egg is held with a holding pipette.
o A very sharp and hollow needle is used to immobilize and pick
up a single sperm.
o This needle is then inserted through the zona (shell of the
egg) and in to the center (cytoplasm) of the egg.
o The sperm is injected in the cytoplasm and the needle is
removed.
o The eggs are checked the next morning for evidence of normal
fertilization.
16
18. Benefits of intracytoplasmic sperm injection
o ICSI enables fertilization to happen when there are very few
sperm available
o Before the development of intracytoplasmic sperm injection,
male infertility related to abnormal semen count was
considered largely untreatable. This assisted reproductive
technique has therefore enabled many couples who would not
previously have been able to conceive, or who would have
been reliant on donor sperm, to conceive a biological child.
18
19. Fertilization and pregnancy success rates with ICSI
o Success is 35% in women aged 30 to 35, 20% at 35 to 40
and less than 10 % after 40. It is better to avail IVF before
it is too late.
o Blastocyst embryo transfer at the 5–6 day stage,
generally has higher success rates than embryo transfer
at the 2–3 day stage.
o depending on the age of the couple, hormone levels,
disease (Diabetes mellitus, hypertension, etc.) and
lifestyle (excess weight, stress, alcohol and nicotine use,
etc.)
19
20. Fertilization and pregnancy success conti…
• IVF with ICSI success rates vary according to the
specifics of the individual case, the ICSI technique
used, the skill of the individual performing the
procedure, the overall quality of the laboratory, the
quality of the eggs, and the embryo transfer skills of
the infertility specialist physician.
20
21. Fertilization and pregnancy success conti…
• In other words, the average egg quantity and quality
tends to be better in ICSI cases (male factor cases)
because it is less likely that there is a problem with the
eggs - as compared to cases with unexplained infertility.
Some unexplained cases have reduced egg quantity
and/or quality - which lowers the chances for a
successful IVF outcome.
21
22. Rates of successful ICSI in vitro fertilization by
season in a recent study
22
22
23. Potential problems with intracytoplasmic sperm
injection.
• The vast majority of children that are born as a result of
intracytoplasmic sperm injection are healthy but there is a
risk that if the father produces abnormal sperm, this may
be due to a genetic trait that is passed on to the child. It
may be possible to test for known genetic diseases
using pre-implantation genetic screening (PGS)but if there
is no obvious genetic abnormality, there is no test.
23
24. Alternative treatment options to IVF with ICSI.
Intrauterine inseminations with the male
partner sperm. This involves sperm
processing in the lab, and then placement
of the processed sperm through the cervix
and up into the uterine cavity using a sterile
catheter by using a sterile catheter.
24
25. References
• NCBI (PUBMED )
• Advanced Fertility Center of Chicago
• Harley Street Fertility Clinic
London, United Kingdom
25
26. Mian Numan kakakhel
M.Phil research scholar NARC (national
agriculture research center Islamabad)
pakistan
email : numanbiotech@yahoo.com
Cell #00923149223397
26