UV- Spectroscopy (Modern Pharmaceutical Analytical Techniques.pptxRAHUL PAL
he Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Spectroscopy is based on the interaction between light and matter.
UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible regions of the electromagnetic spectrum.
Spectroscopy is the study of interaction of electromagnetic radiation with matter. Basic principle of spectroscopy is the Beer-Lambert’s law.
200-900 nm
Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample move from one energy state to another energy state. UV spectroscopy is a type of absorption spectroscopy in which light of the ultra-violet region (200-400 nm) is absorbed by the molecule which results in the excitation of the electrons from the ground state to a higher energy state.Basically, spectroscopy is related to the interaction of light with matter.
As light is absorbed by matter, the result is an increase in the energy content of the atoms or molecules.
When ultraviolet radiations are absorbed, this results in the excitation of the electrons from the ground state towards a higher energy state.
Molecules containing π-electrons or nonbonding electrons (n-electrons) can absorb energy in the form of ultraviolet light to excite these electrons to higher anti-bonding molecular orbitals.
The more easily excited the electrons, the longer the wavelength of light they can absorb. There are four possible types of transitions (π–π*, n–π*, σ–σ*, and n–σ*), and they can be ordered as follows: σ–σ* > n–σ* > π–π* > n–π* The absorption of ultraviolet light by a chemical compound will produce a distinct spectrum that aids in the identification of the compound.
A spectrophotometer is an instrument that measures the amount of light absorbed by a sample. Spectrophotometer techniques are used to measure the concentration of solutes in solution by measuring the amount of the light that is absorbed by the solution in a cuvette placed in the spectrophotometer .
UV- Spectroscopy (Modern Pharmaceutical Analytical Techniques.pptxRAHUL PAL
he Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Spectroscopy is based on the interaction between light and matter.
UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible regions of the electromagnetic spectrum.
Spectroscopy is the study of interaction of electromagnetic radiation with matter. Basic principle of spectroscopy is the Beer-Lambert’s law.
200-900 nm
Spectroscopy is the measurement and interpretation of electromagnetic radiation absorbed or emitted when the molecules or atoms or ions of a sample move from one energy state to another energy state. UV spectroscopy is a type of absorption spectroscopy in which light of the ultra-violet region (200-400 nm) is absorbed by the molecule which results in the excitation of the electrons from the ground state to a higher energy state.Basically, spectroscopy is related to the interaction of light with matter.
As light is absorbed by matter, the result is an increase in the energy content of the atoms or molecules.
When ultraviolet radiations are absorbed, this results in the excitation of the electrons from the ground state towards a higher energy state.
Molecules containing π-electrons or nonbonding electrons (n-electrons) can absorb energy in the form of ultraviolet light to excite these electrons to higher anti-bonding molecular orbitals.
The more easily excited the electrons, the longer the wavelength of light they can absorb. There are four possible types of transitions (π–π*, n–π*, σ–σ*, and n–σ*), and they can be ordered as follows: σ–σ* > n–σ* > π–π* > n–π* The absorption of ultraviolet light by a chemical compound will produce a distinct spectrum that aids in the identification of the compound.
A spectrophotometer is an instrument that measures the amount of light absorbed by a sample. Spectrophotometer techniques are used to measure the concentration of solutes in solution by measuring the amount of the light that is absorbed by the solution in a cuvette placed in the spectrophotometer .
microscope (1).pdf this is a project for for botany majorarpitakhairwar123
Name - Arpita khairwar
Class - B.sc 1st year
Subject - Botany Major
College - Govt. Jayashankar Trivedi College Balaghat
Guided by - Dr. Pratima bisen
Submitted by - Arpita khairwar
While this ppt given by Dr pratima mam this ppt is a educational institution. My ppt is about microscope
Instrumentation of uv visible spectrophotometerTalha Liaqat
A spectrophotometer is an apparatus for measuring the intensity of light in a part of the spectrum, especially as transmitted or emitted by particular substances. The instrumentation of the Spectrophotometer is described in this presentation.
Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. Ultraviolet-Visible (UV-VIS) Spectroscopy is an analytical method that can measure the analyte quantity depending on the amount of light received by the analyte.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
microscope (1).pdf this is a project for for botany majorarpitakhairwar123
Name - Arpita khairwar
Class - B.sc 1st year
Subject - Botany Major
College - Govt. Jayashankar Trivedi College Balaghat
Guided by - Dr. Pratima bisen
Submitted by - Arpita khairwar
While this ppt given by Dr pratima mam this ppt is a educational institution. My ppt is about microscope
Instrumentation of uv visible spectrophotometerTalha Liaqat
A spectrophotometer is an apparatus for measuring the intensity of light in a part of the spectrum, especially as transmitted or emitted by particular substances. The instrumentation of the Spectrophotometer is described in this presentation.
Ultraviolet-visible spectroscopy or ultraviolet-visible spectrophotometry (UV-Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in the ultraviolet-visible spectral region. Ultraviolet-Visible (UV-VIS) Spectroscopy is an analytical method that can measure the analyte quantity depending on the amount of light received by the analyte.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Students, digital devices and success - Andreas Schleicher - 27 May 2024..pptxEduSkills OECD
Andreas Schleicher presents at the OECD webinar ‘Digital devices in schools: detrimental distraction or secret to success?’ on 27 May 2024. The presentation was based on findings from PISA 2022 results and the webinar helped launch the PISA in Focus ‘Managing screen time: How to protect and equip students against distraction’ https://www.oecd-ilibrary.org/education/managing-screen-time_7c225af4-en and the OECD Education Policy Perspective ‘Students, digital devices and success’ can be found here - https://oe.cd/il/5yV
Instructions for Submissions thorugh G- Classroom.pptxJheel Barad
This presentation provides a briefing on how to upload submissions and documents in Google Classroom. It was prepared as part of an orientation for new Sainik School in-service teacher trainees. As a training officer, my goal is to ensure that you are comfortable and proficient with this essential tool for managing assignments and fostering student engagement.
Basic Civil Engineering Notes of Chapter-6, Topic- Ecosystem, Biodiversity Green house effect & Hydrological cycle
Types of Ecosystem
(1) Natural Ecosystem
(2) Artificial Ecosystem
component of ecosystem
Biotic Components
Abiotic Components
Producers
Consumers
Decomposers
Functions of Ecosystem
Types of Biodiversity
Genetic Biodiversity
Species Biodiversity
Ecological Biodiversity
Importance of Biodiversity
Hydrological Cycle
Green House Effect
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
2. SOURCE of LIGHT
2
• Part of the UV and Visible radiation source is Tungsten lamp. See
figure 7.
• UV radiation source is Deuterium
or Hydrogen lamp . See figure 8.
• Range of wavelength 200-400 nm.
FIG.7.Tungsten lamp
FIG.8.Deuterium lamp
6. 2.1.2 MONOCHROMATOR
• It is a device that breaks the polychromatic
radiation into component wavelengths. See figure 9.
FIG.9.Monochromator components.
7. The monochromator unit consists of :
•Entrance slit: defines narrow beam of radiation from source.
•Collimating mirror:(polished surface) collimates the lights.
•Diffraction grating or Prism (make of quartz): disperses the
light into specific wavelength.
•Focusing mirror: captures the dispersed light & sharpens the
same to the sample via exit slit
•Exit slit: allows the corrected wavelength of light to the
sample .
8. 2.1.3 SMPLE SOLIOTION in CUVETTE
• liquid sample is usually contained in a cell called a cuvette. See
figure10.
• Fingerprints and droplets of water disrupt light rays during
measurement.
• Cuvette from Quartz can be used in UV as well as in visible
spectroscopy.
• Cuvette from Glass is suitable for visible but not for UV spectroscopy
because it absorbs UV radiation.
FIG 10.sample solution in cuvette
13. 2.1.4 PHOTO DETECTOR
• A photo detector is a semiconductor device which
converts light energy to electrical energy. It
consists of a simple P-N junction diode and is
designed to work in reverse biased condition. The
photons approaching the diode are absorbed by
the photodiode and current is generated.4 See
figure 11.
FIG 11. Photodiode
14. 2.1.5 READOUT DEVICE.
• Digital screen to record an uv
spectrograph with absorbance against the
wavelength.
-----------------------------------------------
-
2.2 TYPES of SPECTROPHOTOMETER
FIG 12. Types of spectrophotometer.
15.
16.
17.
18.
19.
20.
21.
22.
23.
24.
25.
26.
27.
28.
29. • UV-Vis spectroscopy is used heavily in many different
research areas to identify or quantify a sample.
• Chemical field:
1. Detection of impurities.
2. Structure of organic compounds (single or double
bond, presence or absence of functional group).
3. Kinetics of reaction.
4. Manufacturing drugs.
• Biological fields
1. quantify the amount of protein and DNA in a sample
2. quantify the amount of bacterial cells in a cell culture
2.3 Applications of UV-VIS Spectroscopy.
30. conclusion
• Major advantages of uv-vis spectroscopy are:
1. High sensitivity.
2. Require only small volume of sample.
3. Linearity over wide range of concentration.
4. Can be used with gradient elution.
• Major disadvantages of uv-vis spectroscopy are:
1. Not linear for high concentration.
2. Does not work with compounds that do not absorb
light at this wavelength region.
3. Generates significant heat and requires external
cooling.
59. 2.1.4 PHOTO DETECTOR
• A photo detector is a semiconductor device which
converts light energy to electrical energy. It
consists of a simple P-N junction diode and is
designed to work in reverse biased condition. The
photons approaching the diode are absorbed by
the photodiode and current is generated.4 See
figure 11.
FIG 11. Photodiode
