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In present DNA microarray technology, the different test-samples are pre-tagged with different
kind of fluorescnet tags whose intensity is automatically monitored by a computed software. This
software not only captures the signal from the array, but also quantizes the intensity of this signal
into a calculated number. This number deciphers the extent of expression levels of the gene to
which the fluorphore was attached to.
Here, the most crucial step is to detemine where the signal is coming from and if the signal is
present or not. Since a microarray contains millions of array data points, it is not possible for
normal human eyes to differentiate between two spots based upon their color and intensity. It is
not even required ot perform this function manually because millions of data points cannot be
quantized by human eye. Thus, the software is initially informed the emission and excitation
wavelengths of the fluorophores and thus, the laser equipped in the detection system
automatically records and quantizes the signal intensity without minutest involvement of human.
Thus, is does not matter if the researcher has normal vision or is a color blind, the data
acquisition will be performed solely by the software. Thus it is possible for a person who is
colorblind to successfully interpret a microarray scan becuase the data will be quantized into
numerical format in the end.
Solution
In present DNA microarray technology, the different test-samples are pre-tagged with different
kind of fluorescnet tags whose intensity is automatically monitored by a computed software. This
software not only captures the signal from the array, but also quantizes the intensity of this signal
into a calculated number. This number deciphers the extent of expression levels of the gene to
which the fluorphore was attached to.
Here, the most crucial step is to detemine where the signal is coming from and if the signal is
present or not. Since a microarray contains millions of array data points, it is not possible for
normal human eyes to differentiate between two spots based upon their color and intensity. It is
not even required ot perform this function manually because millions of data points cannot be
quantized by human eye. Thus, the software is initially informed the emission and excitation
wavelengths of the fluorophores and thus, the laser equipped in the detection system
automatically records and quantizes the signal intensity without minutest involvement of human.
Thus, is does not matter if the researcher has normal vision or is a color blind, the data
acquisition will be performed solely by the software. Thus it is possible for a person who is
colorblind to successfully interpret a microarray scan becuase the data will be quantized into
numerical format in the end.

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In present DNA microarray technology, the different test-samples are.pdf

  • 1. In present DNA microarray technology, the different test-samples are pre-tagged with different kind of fluorescnet tags whose intensity is automatically monitored by a computed software. This software not only captures the signal from the array, but also quantizes the intensity of this signal into a calculated number. This number deciphers the extent of expression levels of the gene to which the fluorphore was attached to. Here, the most crucial step is to detemine where the signal is coming from and if the signal is present or not. Since a microarray contains millions of array data points, it is not possible for normal human eyes to differentiate between two spots based upon their color and intensity. It is not even required ot perform this function manually because millions of data points cannot be quantized by human eye. Thus, the software is initially informed the emission and excitation wavelengths of the fluorophores and thus, the laser equipped in the detection system automatically records and quantizes the signal intensity without minutest involvement of human. Thus, is does not matter if the researcher has normal vision or is a color blind, the data acquisition will be performed solely by the software. Thus it is possible for a person who is colorblind to successfully interpret a microarray scan becuase the data will be quantized into numerical format in the end. Solution In present DNA microarray technology, the different test-samples are pre-tagged with different kind of fluorescnet tags whose intensity is automatically monitored by a computed software. This software not only captures the signal from the array, but also quantizes the intensity of this signal into a calculated number. This number deciphers the extent of expression levels of the gene to which the fluorphore was attached to. Here, the most crucial step is to detemine where the signal is coming from and if the signal is present or not. Since a microarray contains millions of array data points, it is not possible for normal human eyes to differentiate between two spots based upon their color and intensity. It is not even required ot perform this function manually because millions of data points cannot be quantized by human eye. Thus, the software is initially informed the emission and excitation wavelengths of the fluorophores and thus, the laser equipped in the detection system automatically records and quantizes the signal intensity without minutest involvement of human. Thus, is does not matter if the researcher has normal vision or is a color blind, the data acquisition will be performed solely by the software. Thus it is possible for a person who is colorblind to successfully interpret a microarray scan becuase the data will be quantized into numerical format in the end.