How would you identify, isolate, amplify, and then clone the gene th.pdf

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how many digit strings of length 4 have at least 2 A. Solve the recurrence relation: a_n = 5a_n - 1 - 6a_n - 2 where a_0 = 0. a_1 = 6. How many digit strings of length 4 have at least two consecutive digits that are the same ? Solution n=4 places to be filled using 0,1,2,3,4,5,6,7,8,9 No of strings having atleast two consecutive digits same = Total number of strings having 4 digits - strings not having any consecutive digits =10*10*10*10 - 10*9*8*7 =10000-5040=4960 ........................................... PLEASE UPVOTE..

Education

How would you identify, isolate, amplify, and then clone the gene that encodes for a specific
protein?
-Use knowledge of PCR, restriction enzymes, primers, vectors, RFLP, NCBI, genomes, and
microarrays.
Solution
The basic purpose of gene isolation is to integrate it with the host cell to get the desired product
usually desired protein. First of all we should know that gene isolation was made possible with
the help of recombinant DNA technology. In the history first time gene was isolated from the
bacteria with the help of bacteriophages. These phage can hybridize with the bacterial DNA. By
isolating the phages we can isolate the gene. This is the fundamental principal of gene isolation.
Bacteriophages infect the bacteria and phage DNA integrates with the bacterial DNA. This
integrated phage DNA is known as prophage, and the bacterium is known as lysogen. Phage
goes two forms of cycles, lysogenic and lytic cycle. Induction will bring about extraction of the
prophage and its multiplication to make numerous progeny, i.e. it enters a lytic phase in which
the bacteria get burst and destroyed. Extraction of prophage is not accurate, it always contain an
additional gene or part of gene of bacteria DNA. When this phage attacks to the new host cell
and it transfer the bacterial gene to the new bacteria. In this way a new gene or allele will
incorporate to the host cell this process is known as transduction.

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Please assit with correcting the code below basesd on the text in Bold. Please read entire post. last attempt to get this right. Thanks The pay calculator still does not calculate the taxes correctly. Remember that only the amount over $600.00 is taxed at 20% - the first $600.00 is taxed at 15%. If the gross is over 600, subtract 600 from the gross and multiply the difference by 0.20 and then add 600 * 0.15 to get the total taxes. It\'s better to multiply by decimal amounts rather than use integers and then divide. You will need to reconsider some of the data types for the variables. int isn\'t the best choice when dealing with monetary amounts. The hours could also contain a decimal. The indentation is not consistently correct but the code includes comments. Instructions Write a program that requests the user to enter hours worked in a week and the hourly wage. The program should then print the gross pay, the taxes, and the net pay. Assume the following: Overtime (in excess of 40 hours for week) = time and a half. Tax rate: 15% of the first $600, 20% of the rest. Code #include int main() { // integer declaration int workhours,hourlywage,taxrate,othour,otwage; //salary, tax, net pay are float variables float salary,tax,netpay; printf(\" Please enter hours worked for the week \ \"); //user enter weekly hours scanf(\"%d\",&workhours); // enter hourly wage printf(\"Please enter your hourly wage \ \"); //user enter hourly rate scanf(\"%d\",&hourlywage); // if hours worked is equal to 40 if(workhours<=40) { salary=workhours*hourlywage; //if salary is less than or equal to 600 if(salary<=600) { //tax rate is equal to 15 taxrate=15; tax=(taxrate*salary)/100; //net pay equals salary minus tax netpay=salary-tax; printf(\"Total gross Pay\\tTax Rate\\t Net Pay\ \"); //print gross pay minus tax rate and total netpay printf(\"$ %f\\t$ %f(15%%) \\t$ %f\ \",salary,tax,netpay); } else { //tax rate equals 20% taxrate=20; tax=(taxrate*salary)/100; //total net pay salary minus tax rate netpay=salary-tax; //print total gross pay, tax rate, and net pay printf(\"Total gross Pay\\tTax Rate\\t Net Pay\ \"); printf(\"$ %f\\t$ %f(20%%)\\t$ %f\ \",salary,tax,netpay); } } else { othour=workhours-40; otwage=hourlywage+(hourlywage/2); salary=(40*hourlywage)+(othour*otwage); if(salary<=600) { taxrate=15; tax=(taxrate*salary)/100; //net pay equals salary minus tax netpay=salary-tax; printf(\"Gross Pay\\tTax Rate\\t Net Pay\ \"); printf(\"$ %f\\t$ %f(15%%) \\t$ %f\ \",salary,tax,netpay); } else { //tax rate equals 20 percent taxrate=20; tax=(taxrate*salary)/100; //net pay equals salary minus tax netpay=salary-tax; printf(\"Gross Pay\\tTax Rate\\t Net Pay\ \"); printf(\"$ %f\\t$ %f(20%%)\\t$ %f\ \",salary,tax,netpay); } } return 0; Solution Find comments inline for fixes. Also made few formatting changes by removing spaces in the output lines. PROGRAM CODE: #include int main() { // integer declaration //changing all int to float float workhours,hourlywage,taxrate,othour,otwag.

PLEASE HELP ME !!IT IS Due Tonight ;(!How can I make the add but.pdf

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