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Base Camp Presentation
How to talk about Redox Signaling
        Gary Samuelson, Ph.D.
Redox Signaling is an Exploding 
           Field of Science




Books
•Redox Biochem
       •Ruma Banerjee              Journals                      Conferences
•Antioxidant and Redox                                           •The Gordon Research 
Regulation of Genes
                                   •Antiox & Redox Signaling
                                                                 Conference on Thiol‐based 
       •Chandan K Sen              •Physiol Genomics             Redox Regulation and Signaling
•Redox Signaling in Biology and    •Am. J. of Physiology         •4th International Conference 
Medicine                                                         on Oxidative/Nitrosative Stress 
       •Claus Jacob
                                   •The J. of Immunology
                                                                 and Disease 
•Redox‐Mediated Signal             •PNAS                         •International Symposium on 
Transduction: Methods and          •Arteriosclerosis, Thromb.    the Pathophysiology of 
Protocols                                                        Reactive Oxygen and Nitrogen 
       •John T Hancock
                                   Vasc.
                                                                 Species 
•Redox Protemics                   •Hundreds More                •Many more
       •Nick Lane
•And More
Signaling Molecules in ASEA Come From 
             Salt and Water
                                           Salt
                                Na+                   Cl -                        Water
                H
                                                                     H

                        O
                                       N          N                          O

                H
                                      Nitrogen                       H
                            REDuction             OXidation

                                                                         H
                                 H
            H                                                                       Super 
Hydrogen                                                                            Oxides
Peroxide                                               O         O
                    O       O
                                       More                                  Cl


  Nitric 
                                      than 20                H
                                                                                     Hypo 
                    N       O
  Oxide                               Molecule                   O                  chlorites 
                                         s …
   …                                                                                  … 
Do we need to become experts in 
Redox Biochemistry?  Answer: No




    Dr. Ruma Banerjee
What are Redox Signaling 
              Molecules?
•   They are produced inside our cells
•   They help cells communicate with each other
•   They help tissues heal themselves
•   The redox signaling molecules in ASEA are 
    actual molecules made from salt and water
How Do Redox Signaling Molecules 
            Work?
• They help cells communicate with each other
• They increase the “bars” on our “cell phones”
• When our cells have a problem, they need to 
  talk to each other
• If they cannot talk the problems become worse
• If they can talk, the problems can be solved
Cells Need to Communicate!!!
   Cell              Cell

             C                 C
             e                 e
             ll                ll



      Mail              Mail
      box               box
Cells are Like Houses that Shelter Life‐
           Sustaining Activity




Fireplace, Furnace, Stove, Heaters   ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐>       Mitochondria
Mail Slots and Sorting  ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐>       Receptors, Vesicles, Golgi
Appliances, Tools, etc.  ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐>       Micromachines
Walls, Floors, Plumbing, Wires ‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐‐>       Membranes, tubules
We can think of Cells as 
 Houses Along Streets
                                                                    C
                                    C                               e
                                    e                               l
                                    l                               l
                                    l
                                                           M
                                                           ai
                           M                                l
                           ai                              b
                            l                              o
                           b                               x
                           o
                           x




                                                                C
                                C                               e
                                e
                                l
                                        Blood                   l
                                                                l
Street
                                l       Vessel
                                                      M
                                                      ai
                      M                                l
                      ai                              b
                       l                              o
                      b                               x
                      o
                      x




                                                                C
                                C                               e
                                e                               l
                                l                               l
                                l
                                                 M
                                                 ai
                 M                                l
                 ai                              b
                  l                              o
                 b                               x
                 o
                 x
Cells live in Communities Where 
They Must Communicate to Survive
When Cells Have a Problem
They Need Emergency Response
                      C                        CC                      C                        C
                      e                        ee                      e                        e
                      l                        ll                      l                        l
                      l                        ll                      l                        l

             M                      M M                       M                       M
             ai                     aiai                      ai                      ai
              l                      ll                        l                       l
             b                      bb                        b                       b
             o                      oo                        o                       o
             x                      xx                        x                       x




                  C                        C                       C                        C
                  e                        e                       e                        e
                  l                        l                       l                        l
                  l                        l                       l                        l

        M                      M
                               M                         M
                                                         M                       M
        ai                     ai
                               ai                        ai
                                                         ai                      ai
         l                      l
                                l                         l
                                                          l                       l
        b                      b
                               b                         b
                                                         b                       b
        o                      o
                               o                         o
                                                         o                       o
        x                      x
                               x                         x
                                                         x                       x




                  C                        C                       C                       CC
                  e                        e                       e                       ee
                  l                        l                       l                       ll
                  l                        l                       l                       ll

   M                      M                         M                      MM
   ai                     ai                        ai                     ai
                                                                            ai
    l                      l                         l                      ll
   b                      b                         b                      b b
   o                      o                         o                      o o
   x                      x                         x                      xx
Redox Signaling Helps Cells Detect the 
         Extent of the Damage
Normal 
Healthy Cells 
Have Fires 
and Smoke 
(Controlled 
Oxidation )

When the 
Fire and 
Smoke Get 
out of 
Controll, 
Cells need 
Help
What Happens When Cells Need 
             Help?
• They must be able to send a “redox” signal
• Redox Signaling Molecules carry the signals
• Fire Marshalls see these “smoke” signals and 
  call in the fire crew
• Fire Crews put out the fires (with antioxidants) 
  and help repair the damage or destroy and 
  rebuild the house
Why Do I Need Redox Signaling 
            Molecules?
• Your body has tens of billions of cells that 
  need repair or replacement every day, even 
  more if you are sick or sore
• As you age and especially if you are sick you 
  do not have enough redox signaling molecules 
  to handle the demand
• If your cells are not able to call for help, they 
  will continue to “burn” (oxidize) and will not 
  be repaired or replaced fast enough
Does Your Brain and Nervous System 
Need to Be Protected and Repaired?
Does Your Heart and Veins Need to be 
             Repaired?
Do Your Muscles Need to Be Repaired?
If You Answered “Yes” to Any of 
These, You Need Redox Signaling 
          Molecules!
Redox Imbalance (Oxidative Stress) 
Signals Protect‐Repair‐Replace Response
Innovation: Infuse Balanced Redox Signaling 
Molecules (Lost by Aging or Stress) back into Tissues
Redox Signaling Technology is Destined 
to Fuel the Greatest Advances in Medical 
          Science This Century
• Redox Signaling is fundamental to all 
  biological multi‐cellular processes
• It is non‐specific, non‐toxic and applicable 
  to a broad range of processes and pathways
• It is at the very core of mediating and 
  regulating the mechanisms that restore 
  functionality and health
Increasing Proper Redox Signaling in Your 
  Body is the Single Most Important Thing 
        You Can Do For Your Health
• Redox Signaling is absolutely necessary for all 
  living things on earth with more than one cell
• Redox Signaling Molecules in proper balance 
  cannot hurt you, they are in all of your cells 
  already, you just need more of them to keep 
  the cells healthy
• You need Redox Signaling Molecules so that 
  your body can restore and maintain its health
Stable Balanced Redox Signaling 
     Molecules in ASEA Confirmed
• ASEA contains a balance of ROS and RS 
• Fluorescent Indicators Detect ROS 
  Components in ASEA
• ESR and EPR methods were used to verify 
  existence of stable ROS components
• Low Temperature Mass Spectroscopy used 
  find several ionic components of ASEA
ASEA Generates and Enhances 
 Antioxidants with Zero Toxic Effects 
• ASEA causes no signs of toxicity on human 
  cells, even on a genetic level
• ASEA causes a significant increase (500%) in 
  the effectiveness of Antioxidants
• ASEA causes enhanced detection of damaged 
  cells
• ASEA causes increased messenger sensitivity
• ASEA causes old (senescent) cells to revive
Scientists Have Proven that There Are 
 Redox Signaling Molecules in ASEA
• ASEA contains the same balance of Redox 
  Signaling Molecules that already exist in the fluids 
  inside your body, cells and tissues
• Complex analysis has been done to prove that 
  ASEA has Redox Signaling Molecules and that 
  they are safe
• ASEA helps put out the fires in your body, helps 
  the body restore the cells proper function and 
  causes damaged cells to revitalize
Groundwork Toward Redox Signaling 
         Development
• Can stable complexes of Redox Signaling 
  Molecules be manufactured?  YES.
• Can the bioactivity of such be 
  characterized?  YES.
• Can such complexes be non‐toxic and 
  readily absorbed?  YES.
• Can such a technology be commercialized? 
  It is up to you!
The Most Effective Way to Take ASEA
• Pour into a clean glass measuring cup
• 2 oz. twice a day – maintenance dose
• 4 oz. twice daily – mild challenges
• 8 oz. twice daily – severe challenges
• ½ oz twice daily or spray in mouth – reduce 
  detox symptoms
• Do not eat 5 min. before or after taking ASEA
Can We Tell the Story of the 
Science of ASEA So That Everyone 
Can Understand It and Repeat it?

 If we can ASEA will make it into 
 more of the hands that need it

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