This document summarizes research on using high intensity pulsed electric fields (PEF) to preserve liquid foods as an alternative to thermal pasteurization. It investigates how electrical field strength, total pulse energy input, and treatment temperature impact microbial inactivation. Experiments were conducted with bacteria (E. coli, B. megaterium, L. innocua) and yeast (S. cerevisiae) using stainless steel and carbon electrodes. The results indicate that higher field strengths are needed to effectively inactivate smaller cells and cells in aggregates. Temperatures over 40 °C were also found to increase the effectiveness of PEF. An enthalpy balance analysis of a hypothetical PEF process that heats product prior to treatment is
Meta-genomics is the application of modern genomics techniques to the study of communities of microbial organisms directly in their natural environments, bypassing the need for isolation and lab cultivation of individual species”
MICROBIAL FUEL CELL (MFC) TECHNOLOGY FOR HOUSEHOLD WASTE REDUCTION AND BIOENE...civej
MFC is a bioreactor, extracts chemical energy from organic compounds, directly as electrical energy,
through microbial degradation under anaerobic conditions. The main objective of the current study is to
compare the degradation ability and corresponding electric potential development from different
household substrates using lab scale MFC. 50hr batch experiments were conducted with household
organic rich substrates like coconut water, rice starch and milk. Different concentrations of KMnO4were
used as oxidizing agent in the cathode chamber. A voltage of about 300to 700mV was produced from
125ml of substrates seeded with cow dung. Coconut water and starch produced electric potential with the
support of oxidizing agent KMnO4, where as the potential produced by milk found to be independent of the
KMnO4concentration. The maximum electric potential developed was 762mV from coconut water at
1500mg/l KMnO4with a COD reduction of 22%.
Protein engineering and its techniques himanshuhimanshu kamboj
b pharma 6th sem
pharmaceutical biotechnology
Protein engineering
Objectives of protein engineering
Rationale of protein engineering
Protein engineering methods
Rational design -site-directed mutagenesis methods
Advantages and disadvantages of rational design
Directed evolution -random mutagenesis
Advantages and disadvantages of directed evolution
Peptidomimetics
Classification of peptidomimetics
Advantages and disadvantages of peptidomimetics
Flow cytometry
Instrumentation
Principle
components
Meta-genomics is the application of modern genomics techniques to the study of communities of microbial organisms directly in their natural environments, bypassing the need for isolation and lab cultivation of individual species”
MICROBIAL FUEL CELL (MFC) TECHNOLOGY FOR HOUSEHOLD WASTE REDUCTION AND BIOENE...civej
MFC is a bioreactor, extracts chemical energy from organic compounds, directly as electrical energy,
through microbial degradation under anaerobic conditions. The main objective of the current study is to
compare the degradation ability and corresponding electric potential development from different
household substrates using lab scale MFC. 50hr batch experiments were conducted with household
organic rich substrates like coconut water, rice starch and milk. Different concentrations of KMnO4were
used as oxidizing agent in the cathode chamber. A voltage of about 300to 700mV was produced from
125ml of substrates seeded with cow dung. Coconut water and starch produced electric potential with the
support of oxidizing agent KMnO4, where as the potential produced by milk found to be independent of the
KMnO4concentration. The maximum electric potential developed was 762mV from coconut water at
1500mg/l KMnO4with a COD reduction of 22%.
Protein engineering and its techniques himanshuhimanshu kamboj
b pharma 6th sem
pharmaceutical biotechnology
Protein engineering
Objectives of protein engineering
Rationale of protein engineering
Protein engineering methods
Rational design -site-directed mutagenesis methods
Advantages and disadvantages of rational design
Directed evolution -random mutagenesis
Advantages and disadvantages of directed evolution
Peptidomimetics
Classification of peptidomimetics
Advantages and disadvantages of peptidomimetics
Flow cytometry
Instrumentation
Principle
components
Pulsed electric field (PEF) processing is an emerging non-thermal food preservation technology. PEF technology is established on the utilization of electric fields to remove food-borne pathogens and to subjugate the spoilage microorganisms in foods. This technology is notably acknowledged for its capability to amplify the mean life of food products without the utilization of heat also preserving the quality aspects such as sensory and nutritional attributes, together with enabling the safety of food products
FDA - kinetics of microbial inactivation for alternative food processing tech...Wouter de Heij
More information on PEF and PEF 2.0 (PurePulse) can be found on:
http://www.purepulse.eu or twitter @pure_pulse
http://en.topwiki.nl/index.php/PurePulse_-_PEF_2.0
and pinterest: http://pinterest.com/toptechtalks/purepulse-pef-2-0/
Kinetics of microbial inactivation for Pulsed Electric Field (PEF, PurePulse)...CoolWave Processing b.v.
Background document of the the FDA regarding PEF. Our PurePulse technology is the second generation PEF and first successful industrialized PEF-technology.
More information can be found on:
www.cwp-bv.nl
www.purepulse.eu
http://www.pinterest.com/toptechtalks/purepulse-pef-20/
Non thermal processing of food- Pulsed electric field and visible lightT. Tamilselvan
In this presentation i gave an intro to non thermal processing methods in food and their advantages and disadvantages and have brief working of Pulsed electric field and pulsed visible light over other methods.
High voltage pulse technique or High intensity pulsed electric field processing involves the application of electric pulses of high voltage
20-80 kV/cm to the food placed between two electrodes.
The applied electric field create a pores on the cell membrane, thus the phenomenon known as Electroporation or electropermeabilization.
The effect of Electroporation can be divided into four steps:
An increase in the transmembrane potential
Pore formation
Evolution of the number and size of the pores
Pore resealing
Pulsed Electric Field (PEF) applications can be utilised to achieve disintegration of biological tissues or microbes. Various applications have been identified such as improvement of mass transfer during extraction or drying as well as gentle food preservation. The first commercial applications of the technique have been achieved. By development of equipment based on state of the semiconductor, equipment reliability and cost effectiveness of the equipment has been improved. The technology is heading for wider industrial application.
More information can be found on:
- www.purepulse.eu
- http://www.pinterest.com/toptechtalks/purepulse-pef-20/
- http://en.topwiki.nl/index.php/PurePulse_-_PEF_2.0
Pulsed electric field (PEF) processing is an emerging non-thermal food preservation technology. PEF technology is established on the utilization of electric fields to remove food-borne pathogens and to subjugate the spoilage microorganisms in foods. This technology is notably acknowledged for its capability to amplify the mean life of food products without the utilization of heat also preserving the quality aspects such as sensory and nutritional attributes, together with enabling the safety of food products
FDA - kinetics of microbial inactivation for alternative food processing tech...Wouter de Heij
More information on PEF and PEF 2.0 (PurePulse) can be found on:
http://www.purepulse.eu or twitter @pure_pulse
http://en.topwiki.nl/index.php/PurePulse_-_PEF_2.0
and pinterest: http://pinterest.com/toptechtalks/purepulse-pef-2-0/
Kinetics of microbial inactivation for Pulsed Electric Field (PEF, PurePulse)...CoolWave Processing b.v.
Background document of the the FDA regarding PEF. Our PurePulse technology is the second generation PEF and first successful industrialized PEF-technology.
More information can be found on:
www.cwp-bv.nl
www.purepulse.eu
http://www.pinterest.com/toptechtalks/purepulse-pef-20/
Non thermal processing of food- Pulsed electric field and visible lightT. Tamilselvan
In this presentation i gave an intro to non thermal processing methods in food and their advantages and disadvantages and have brief working of Pulsed electric field and pulsed visible light over other methods.
High voltage pulse technique or High intensity pulsed electric field processing involves the application of electric pulses of high voltage
20-80 kV/cm to the food placed between two electrodes.
The applied electric field create a pores on the cell membrane, thus the phenomenon known as Electroporation or electropermeabilization.
The effect of Electroporation can be divided into four steps:
An increase in the transmembrane potential
Pore formation
Evolution of the number and size of the pores
Pore resealing
Pulsed Electric Field (PEF) applications can be utilised to achieve disintegration of biological tissues or microbes. Various applications have been identified such as improvement of mass transfer during extraction or drying as well as gentle food preservation. The first commercial applications of the technique have been achieved. By development of equipment based on state of the semiconductor, equipment reliability and cost effectiveness of the equipment has been improved. The technology is heading for wider industrial application.
More information can be found on:
- www.purepulse.eu
- http://www.pinterest.com/toptechtalks/purepulse-pef-20/
- http://en.topwiki.nl/index.php/PurePulse_-_PEF_2.0
Recent Advances in Dairy Industry -Chirag Prajapati.pptxChirag Prajapati
Embark on a journey through the realm of "Recent Advances in Dairy Processing" with this enlightening PowerPoint presentation. Discover the cutting-edge technologies and innovations transforming the dairy industry. Learn about novel processing methods, advanced equipment, and sustainable practices that are revolutionizing milk and dairy product manufacturing. From ultra-high-temperature processing (UHT) to membrane filtration and beyond, this presentation highlights the latest developments that enhance efficiency, quality, and sustainability in dairy processing.
Stikstof beleid in NL v1 - Food4Innovations.pdfWouter de Heij
Voorstel:
- Ga terug naar emissie beperking.
- Stop met gebruik Aerius in wetgeving/vergunning aanvragen.
- Deel Nederland op in regios.
- Ontkoppel NOx met Ammoniak
- Kom met duidelijke concrete maatregelen.
Almost 80 years old, and therefore long retired. My father - Harrie de Heij - is one of the designers of southern Flevoland and Almere. It was 'his project' all his life. Recently also a short interview: https://youtu.be/lDK53MunI-I
I became an entrepreneur. The agreement? Making something from nothing. Draw stripes on a blank sheet of paper and then get to work. That binds us.
Also see: https://food4innovations.blog
De duivel in ons politieke systeem - Wouter de Heij over modellen (Foodlog)Wouter de Heij
See full discussion:
https://www.foodlog.nl/artikel/modellen-zijn-de-duivel-in-ons-politieke-systeem/
and future articles on:
https://food4innovations.blog
10 vragen en antwoorden over Methaan, een kort levend broeikasgas (WUR).Wouter de Heij
Meer over herkauwers en methaan:
https://food4innovations.blog/2022/08/08/de-methaan-emissie-van-koeien-is-onderdeel-van-de-korte-c-cyclus-en-dus-niet-te-vergelijken-met-de-verbranding-van-aardgas-steenkolen-of-aardolie-dat-in-de-lange-cyclus-zit-an-inconvenient-truth/
Methaan balans:
https://food4innovations.blog/2017/07/04/de-vergroening-van-ons-energiesysteem-nodig-absoluut-maar-wat-is-nu-wijsheid-1x-de-methaan-balans/
En recente literatuur:
https://aleph-2020.blogspot.com/2019/06/greenhouse-gas-emissions.html
More fuel for the food-feed debate (FAO , 2022)Wouter de Heij
See also:
https://www.foodlog.nl/artikel//twee-gebieden-en-twee-gedachten-over-eiwitproductie-voor-mensen/allcomments/#comment-323767
And
https://www.food4innovations.blog
See also:
https://www.foodlog.nl/artikel//twee-gebieden-en-twee-gedachten-over-eiwitproductie-voor-mensen/allcomments/#comment-323727
and www.food4innovations.blog
COVID Risico's : basis achtergrond en over besmet en besmettelijkWouter de Heij
Achtergrondslides over corona, uitleg over hoe je een risico-analyse uitvoert en toelichting op besmet zijn, en besmettelijkheid en antwoord op de vraag waarom antigeen testen wel zinvol zijn.
zie ook blog-post:
zie ook:
www.food4innovations.blog
www.top-bv.nl
www.boxnb.nl
The Future of Food in het aankomende post-covid tijdperk (December 2020)Wouter de Heij
Live luisteren en vragen stellen op:
https://www.youtube.com/watch?v=gqi7unhcUOs&fbclid=IwAR11ejphFvR2dLpqbizwX0_erHfK-g82IkYsix-ve0gOPCh3QUvy0yBvzfI
Zie ook de drie afzonderlijke delen van deze keynote op YouTube:
Part1: https://youtu.be/XbPxPChzLQc
Part2: https://youtu.be/rx5tNFcC1Qw
Part3: https://youtu.be/adUvFNycHc0
Meer weten?
www.top-bv.nl
www.boxnv.nl
www.food4innovations.blog
of stuur een mail naar info@top-bv.nl
See also:
https://food4innovations.blog/2020/03/16/live-blog-over-de-corona-crisis-covid-19-dagelijkse-beschouwingen-van-wouter-de-heij-food4innovations/
and
https://food4innovations.blog/2020/03/22/corona-covid-19-en-andere-virussen-kanaal-op-youtube-food4innovations/
See also:
https://food4innovations.blog/2020/03/16/live-blog-over-de-corona-crisis-covid-19-dagelijkse-beschouwingen-van-wouter-de-heij-food4innovations/
and
https://food4innovations.blog/2020/03/22/corona-covid-19-en-andere-virussen-kanaal-op-youtube-food4innovations/
Dynamics and Control of Infectious Diseases (2007) - Alexander Glaser Wouter de Heij
See also:
- https://food4innovations.blog/2020/03/26/montecarlo-simulaties-tonen-aan-wat-de-onzekerheid-is-en-dat-we-minimaal-1600-maar-misschien-wel-2000-2500-ic-plaatsen-nodig-hebben/
See also:
https://food4innovations.blog/2020/03/16/live-blog-over-de-corona-crisis-covid-19-dagelijkse-beschouwingen-van-wouter-de-heij-food4innovations/
and
https://food4innovations.blog/2020/03/22/corona-covid-19-en-andere-virussen-kanaal-op-youtube-food4innovations/
Demographic science aids in understanding the spread and fatality rates of CO...Wouter de Heij
See also:
https://osf.io/fd4rh/?view_only=c2f00dfe3677493faa421fc2ea38e295
and live-blog:
https://food4innovations.blog/2020/03/16/live-blog-over-de-corona-crisis-covid-19-dagelijkse-beschouwingen-van-wouter-de-heij-food4innovations/
Infectious disease modelling - the math behind CoronaWouter de Heij
See. also live blog at:
https://food4innovations.blog/2020/03/16/live-blog-over-de-corona-crisis-covid-19-dagelijkse-beschouwingen-van-wouter-de-heij-food4innovations/
2. 538 S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546
Despite the extensive knowledge in food preservation by heat with several sections of capacitors and inductive elements have
treatment [7,8] and despite continued attempts to improve the to be used. In this case the pulse generation system, in particular
quality of processed foods [9] there is still a need for technolo- the impedance of the line has to be adapted to the resistive load
gies that minimize the destructive influence of heat on valuable of the treatment chamber. A comparison of energy performance
food compounds [10]. Even more recent concepts like high- of different pulse generation systems has been conducted by
temperature-short-time processing fail if heat transfer and/or De Haan et al. [15], concluding an exponential decay system
heat penetration is limited by intrinsic thermophysical proper- will not exceed an energy efficiency of 38%. They compared
ties of the product. Since the thermal energy which is required square wave pulses with a certain peak voltage Vpeak and
to destroy the contaminating microorganisms has to be trans- duration Tp to exponential ones by fitting blocks of Vpeak and
mitted across the product itself, the design of fast and uniform Tp under an exponential pulse, assuming that excess voltage of
heating and cooling steps is one of the primary challenges of the exponential pulses results in excess losses. Several studies
industrial preservation by heat. Most of the thermal processing investigating the relation between field strength and increase
equipment in use consists of systems transfering heat across an in inactivation have shown that in particular for microbial cells
interface driven by a temperature gradient. On the product side with small diameter or for media containing particles higher
only the convective heat transport can be enhanced by external field strength leads to a better inactivation efficiency [16–19].
measures, i.e. by the generation of turbulent flow. Compared to Since PEF-processing on an industrial scale will be applied
thermal inactivation the destruction of microbes by electropora- for continuous product flow, the efficiency of the treatment
tion shows no time delay with respect to the propagation of the strongly depends on the design of the flow-through treatment
lethal treatment intensity. chamber which basically is composed of two electrodes and an
insulating body. Various treatment chamber designs as parallel
2. Theory plates, coaxial cylinders or co-linear configurations have been
used for PEF processing [20]. The applicability of a particular
In electrically conductive food placed between a high voltage design is determined by several crucial properties of the cham-
and a grounded electrode the resulting electrical field can be pre- ber. To achieve a sufficient treatment intensity for all volume
dicted from the Laplace equation 2 ϕ = 0, where ϕ denotes the elements as well to prevent over-processing or arching the elec-
electrical potential. The potential difference ϕM at the mem- tric field should be free of local intensity peaks. A co-linear
brane of a biological cell with spherical shape and a radius R configuration is producing a flow pattern which is desirable for
induced by the external electrical field E can be approximated food processing and cleaning in place. In such chambers hol-
by Eq. (1) which is derived from solving Maxwell’s equations low high voltage and grounded electrodes with circular inner
in ellipsoidal coordinates assuming several simplifying [11] hole are kept on a well defined distance by an insulating spacer.
The product is pumped through the drilling forming the elec-
ϕM = −f (A)AF E (1) trical load of the high voltage discharge circuit. Relative to the
This formula yields the local membrane potential difference electrodes, the inner diameter of the insulator should be slightly
at the distance AF from the centre in direction of the external pinched in order to produce a more homogeneous electrical field
electrical field. The shape factor f(A) is a function of the three [21]. The treatment chamber configuration determines the resis-
semi-axis (A1 , A2 , A3 ) of elliptical cells tive load and therefore the properties of the discharging circuit.
The load resistance of a chamber is dependent on the conductiv-
2 ity of the treated media, resulting in limitations in the range of
f (A) =
∞ 3 media conductivity to which the electric pulses can be applied.
2 − A1 A2 A3 0 1/ (s + A2 )
F n=1 s + A2
n ds
(2)
3. Experimental
In literature [12] it is reported that in excess of a critical 3.1. PEF treatment system
transmembrane potential ϕM of 1 V a rapid electrical break-
down and local conformational changes of bilayer structures The exponential decay pulse system used consists of a vari-
occur. Considering a membrane thickness of 5 nm this translates able storage capacity of 6.8–27.2 nF charged by a high voltage
to a critical membrane field strength Ecrit of 2000 kV cm−1 ! A charging unit. A thyristor switch (HTS 240-800SCR, Behlke
drastic increase in permeability re-establishes the equilibrium Electronic GmbH, Germany) is used to discharge the total stored
of the electrochemical and electrical potential differences of the energy across a protective resistor and the treatment chamber to
cell plasma and the extracellular medium. Although many dif- ground. The pulse raise time typically was in the range between
ferent waveforms are applicable for PEF technology, the pulse 80 and 120 ns, the pulse width, defined as the time needed to
shapes commonly used are either exponential decay or square decrease the voltage to 37% of its peak value between 1.5 and
wave pulses. Square wave generating systems require a switch 10 s, depending on the number of capacitors, the load voltage
with turn-off capability or a pulse forming network. As switches and the electrical properties of the media. The pulse frequency
with turn off capability are hardly available for high power was varied between 1 and 100 Hz. For a treatment of ringer solu-
applications systems [13], serial or parallel connections of tion with a conductivity of 1.5 mS cm−1 , a capacity of 27.2 nF,
switches [14] or lumped or distributed pulse forming networks a load voltage of 12 kV at an initial treatment temperature of
3. S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546 539
45 ◦ C the pulse width was 6.2 s. At a flow rate of 5 kg h−1 the megaterium and Malt Extract Agar (Merck KgaA, Darmstadt,
medium residence time in one treatment zone with a diameter of D) for S. cerevisiae. These plates were incubated for 24 h at
4 mm and a length of 6 mm (within the insulator) was 0.06 s, the 37 ◦ C, S. cerevisiae was incubated for 48 h at 30 ◦ C. All micro-
medium pulse number per volume element was 6 at a frequency bial analysis were done in duplicate, the lower detection limit
of 50 Hz, translating to an energy input of 60 kJ kg−1 . was 20 CFU ml−1 . The inactivation of vegetative organisms was
A micro toothed wheel pump (mzr 4600, HNP-Mikrosysteme evaluated by calculating the log reduction in viable cell counts
GmbH, Parchim, Germany) was used to pump the media through compared to the untreated sample.
silicone tubes to a coiled metal tube inversed into a heating bath,
the treatment chamber and a cooling coil. The mass flow rate was 3.3. Calculation of Cook- and PU-value
set between 2 and 7 kg h−1 . The calculated mean residence time
in the treatment chamber (not the treatment zone) is 1 s at a flow The Cook (C)-value, a key benchmark for the thermal load
rate of 5 kg h−1 , 25 s in the heating and 15 s in the cooling sys- and degradation of ascorbic acid and flavor during a treatment
tem, calculated by the unit volume and the flow rate. The total with variable temperature-time-regime changes can be calcu-
residence time was approximately 31 s. The media was heated lated using Eq. (4) with a medium z-value of 25 ◦ C [23] for
from the temperature Tin,1 to the initial treatment temperature quality losses and a reference temperature of 100 ◦ C
Tin,2 in the range of 35–75 ◦ C. The maximum temperature was t
in the range of 45–80 ◦ C, after treatment the media was cooled to C-value = 10T −Tref /z dt (4)
Tout below 20 ◦ C. Treated samples were collected in Eppendorf- 0
cups and placed on ice immediately. Due to the short residence The pasteurization unit (PU), a key benchmark for thermal
times at high temperatures the thermal load of the media is very load in relation to microorganisms can be calculated using Eq.
low. For acquisition of voltage and current at the treatment cham- (5) with a z-value of 10 ◦ C and a reference temperature of 80 ◦ C
ber a 400 MHz digital oscilloscope, a 75 MHz high voltage and [24]. The z-value is the increase or decrease in temperature
a 100 MHz current probe are used. The specific energy input required to increase the decimal reduction time by one order
wspecific can be calculated by Eq. (3), where E, κ(T), f and denote of magnitude
the electric field strength, the media conductivity, the repetition t
rate and the mass flow rate, respectively PU = 10T −Tref /z dt (5)
0
1 ∞
wspecific = f κ(T )E(t)2 dt (3)
m
˙ 0 4. Results and discussion
3.2. Sample preparation and microbial analysis 4.1. Impact of cell size and orientation
E. coli K12 DH5␣, Saccharomyces cerevisae DSM 70451, In Fig. 1 Eqs. (1) and (2) have been used to theoretically
Bacillus megaterium DSM 322 and Listeria innocua NCTC predict the external electrical field E required to induce a trans-
11289 where obtained from the German culture collection membrane potential ϕM = 1 V which in many publications
(DSM) Braunschweig. E. coli was chosen as main indicator for
microbial inactivation as outbreaks of E. coli O157:H7 infec-
tions were observed in the USA after consumption of apple
juice in 1996. Inocula were prepared from stock cultures 24 h
before each experiment by inoculating 50 ml of nutrition broth.
As media were used: Standard I Nutrient Broth (Oxoid, Bas-
ingstoke, UK) for E. coli and B. megaterium, Tryptose-Soy-
Broth (Oxoid, Basingstoke, UK) for L. innocua and Malt Extract
Broth (Merck KgaA, Darmstadt, D) for S. cerevisae. Cells were
incubated for 24 h at 37 ◦ C to obtain cultures in stationary growth
phase. Before PEF treatment, 10 ml L−1 cell suspension were
added to the treatment media. As treatment media ringer solution
diluted to a temperature dependent electrical conductivity in the
range of 1.25–1.5 mS cm−1 was used. (CG 858, Schott Geraete
Fig. 1. Impact of orientation of ellipsoidal microorganisms relative to the elec-
GmbH, Hofheim, D). Before treatment a sample of untreated trical field E. At a cell specific threshold level the field strength inside the
media was taken to determine the initial cell number. cell membrane exceeds a threshold level Ecrit . Those cells are electroporated
The collected samples were placed on ice immediately after which have their longer semi-axis in parallel to E. Other orientations require
treatment and dilutions were made right after finishing the field strengths in excess of Ecrit . By Eqs. (1) and (2) the required external field
strength has been calculated for all spacial orientations. Three organisms, dif-
experiment using sterile Standard I Nutrient Broth (Oxoid, Bas-
ferent in geometry have been chosen as examples (characteristical dimensions
ingstoke, UK). Viable counts of vegetative cells were determined from Bergey [25]). The chart on the left shows the fraction of cells which have
using drop plating method [22] on Standard I Nutrient Agar an orientation which does not cause electroporation in response to the given
(Merck KgaA, Darmstadt, D) for E. coli, L. innocua and B. external field strength.
4. 540 S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546
is regarded to be the precondition for irreversible membrane is predicted to be sufficient to lethally damage most of the organ-
breakdown and cell death in response to single pulse treat- isms in a bacterial suspension exposed to PEF.
ment. Exemplary, three microorganisms have been chosen which
are considerably different in shape and in size, characteristi- 4.2. Electric field strength distribution
cal dimensions have been taken from Bergey [25]. The three
semi-axes (A1 , A2 , A3 ) define the geometry of the equivalent The electrode configuration of the colinear treatment cham-
ellipsoidal bodies used to calculate the shape factor f(A). AF in ber is presented schematically in Fig. 2. One cylindrical central
Eqs. (1) and (2) denotes the length of the semi-axis in direc- high voltage electrode made from stainless steel with an inner
tion of the external field E. In Fig. 1, top right, this is shown drilling diameter of 6 mm has two grounded counterparts at the
schematically for two-dimensional situations. The diagram on inlet and outlet of the cuvette separated by insulating ceram-
the left side in Fig. 1 shows the fraction of cells in populations of ics, producing a gap of 6 mm. For reason of symmetry only for
microorganisms randomly distributed in orientations which did the upper half of the treatment chamber is the field distribution
not reach the critical membrane field strength Ecrit and, hence, shown. Depending on the position inside the treatment zone,
will not be electroporated. Above the orientation of cells the the peak field strength can take different levels. For numerical
natural size variation between different cells of a strain has to simulation of the electric field strength in the treatment zone the
be taken into account. Quickfield® FEM code (Tera Analysis Ltd., Denmark) has been
It is evident that larger cells are more susceptible to electrical used, the modelation is shown in Fig. 2. For the central axis
fields. Yeast cells of S. cerevisiae are affected already at ca. (between position A and B) the spacial distribution is plotted
2 kV cm−1 if the longer semi-axis A1 is directed in parallel to for the specified treatment conditions (Fig. 2, bottom). Along
the external electrical field E. However, a field strength higher this line, the maximum field strength is located in the mid posi-
than 2 kV cm−1 is needed to affect also those cells having less tion of the insulator. In combination with the flow pattern of the
favorable orientations. For yeast it is evident that most of the product, it has to be ensured that all volume elements flowing
organisms are hit when the external field applied is in excess of through the chamber receive a lethal dose of electrical energy
4 kV cm−1 . [21]. Due to the process specific pulsed energy delivery this is
In contrast, smaller cells like L. innocua require 15 kV cm−1 of most importance for the efficiency of the PEF process.
in minimum and theoretically more than 35 kV cm−1 to bring In addition the theoretical lethal effect of PEF may be
about extensive microbial inactivation. Most of the other bacteria impaired by agglomerations among microorganisms or between
relevant for food preservation are located between the curves of microbes and insulating particles present in the food. This effect
Saccharomyces and Listeria. For E. coli for example 15 kV cm−1 was investigated by a 2D modeling of the electric field dis-
Fig. 2. Features of co-linear PEF treatment chambers. Top: sectional view of the electrode configuration and the resulting electrical field. The central high voltage
electrode is separated from two grounded electrodes on either side by an electrical insulator. One example is given which shows the field strength along the central
axis at the treatment zone A–B.
5. S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546 541
Fig. 4. Electric conductivity of different liquid food systems as function of the
temperature, determined by conductivity measurement.
Fig. 3. Factors which may cause inhomogenities in the electrical field—top: in the field of electroporation [12,27]. This approach is justified
agglomeration of two bacterial cells; bottom: bacterial cell attached to a fat for most of the in-use pulsed power applications in food process
globule. The external electrical field strength is 12.5 kV cm−1 . The white ring engineering. However, modifications of electric field treatment
shows the location on the membrane where the pore-formation is expected to
using, e.g., pulsed radio frequencies [28], may yield completely
happen when the field strength is high enough to produce 1 V membrane poten-
tial difference (=2000 kV cm−1 ). However, due to the configurations shown, in different results regarding threshold field strength and pulse
both cases the required critical membrane potential Ecrit is not reached. Finite energy requirements.
elements modeling has been performed by the Software Quick Field (Tera Anal- The conductivity of liquid food, caused by the presence of
ysis Ltd., Denmark). ions, has been determined for different liquid food by a conduc-
tivity measurement (CG 858, Schott Geraete GmbH, Hofheim,
D) and shows a linear relation to temperature (Fig. 4). During
tribution to approximate the degree of field perturbation. In the electrical discharges temperature is increasing dependent
Fig. 3 the membrane potential of small bacterial cells (simi- on energy input in the respective volume element, leading to
lar to Listeria) was simulated by application of the Quickfield® changes in media conductivity and electric field distribution.
FEM code (Tera Analysis Ltd., Denmark). The exposure to Besides that the total resistance of the treatment chamber, deter-
an external electrical field of 12.5 kV cm−1 which can induce mined by the electrode configuration and the conductivity of
a critical electrical field strength Ecrit of 2000 kV cm−1 ori- the liquid media determines the voltage drop across it. If the
ented along the electric field lines and without presence of resistance of connectors and protective resistor is in the same
agglomerations. The white rings show the location where the range than that of the treatment chamber there will be a high
membrane field strength is maximized. The reduced peak val- voltage drop, leading to a reduced peak voltage across the elec-
ues of 0.84 and 0.45 Ecrit show that agglomeration of cells as trodes. Adaptation of the limiting, protective resistors to low
well as the presence of insulating particles can strongly reduce resistance systems might be difficult, as most of the available
the lethality of the PEF process, as the required membrane switching systems and in particular semiconductor switches
potential for an electroporation is not achieved. Variations of require a maximum current limitation in case of short circuit.
cell orientation, and in particular the presence of particles or Usage of a treatment chamber with a high resistance like a
fat globules as well as formation of clusters might result in co-linear electrode configuration with a resistance in the range
an even lower factors as 0.45. Dependent on the properties of of several hundred Ohm results in a more effective voltage
the food matrix and its contents a worst case scenario has to division and a higher electric field strength can be achieved
be developed to choose an appropriate electric field strength to even with highly conductive treatment media. The electric field
excess the critical transmembrane potential for all cells. Elec- strength E, which is easily determined in the case of parallel
trically insulating gas bubbles which may be produced at the plate electrode configuration was calculated by the measured
electrode by electrolysis can cause a similar weakening effect peak voltage U multiplied with a cell factor of 1.33, repre-
[26]. senting the electric field strength in the center of the treat-
In this article we focus on membrane permeabilization in ment zone. This factor was determined by modeling the electric
response to high intensity pulsed electric fields. All considera- field strength for this particular co-linear electrode configuration
tions and conclusions are based on the ‘classical’ research work (Fig. 2).
6. 542 S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546
From a processing point of view this behavior may be
exploited by splitting the total required energy input into (recov-
erable) thermal energy which makes the microbes more suscep-
tible to PEF and electrical pulse energy which brings about the
electroporation [29].
4.4. Impact of different electrode materials
As electrode material stainless steel is commonly used, but
problems with electrolysis, formation of deposits, electrode cor-
rosion and transfer of particles into the treated media have been
reported [32–34]. To avoid electrochemical reactions also other
materials like platin or metal oxides [35] or polymer coatings
have been suggested [36]. Faradaic reactions taking place at the
interface electrode|media may result in partial electrolysis of
the treated media as well as in electrode corrosion. Transfer-
ring electrical energy across this interface leads to formation of
charged double layers at the electrode surface. Using steady con-
ditions, as used for electrolysis this layers remain charged during
the whole process, acting as capacitance. The transferred current
Fig. 5. Inactivation of E. coli in ringer solution in relation to specific energy
input at different treatment temperatures, an electric field strength of 16 (– – –) flows via the Faradaic impedances in parallel to the double layer
and 20 kV cm−1 (- - -) and a flow rate of 5 kg h−1 . Results are means based on capacitance leading to Faradaic redox reactions at the interface
data from two experiments, standard deviations are shown by error bars, the [37]. To transfer a high amount of energy avoiding Faradaic
initial count number was 1.58 × 107 CFU ml−1 . processes it is sufficient that the potential drop across each dou-
ble layer capacitor remains smaller than the threshold voltage
above which significant electrochemical reactions occur. Under
4.3. Impact of different process parameters such circumstances the current flow would be purely capaci-
tive, avoiding oxidative and reductive reactions at the electrode
The effect of initial treatment temperature, electric field interfaces. To minimize the extent of this reaction to a tolerated
strength and specific energy input on inactivation of E. coli in maximum level the treatment chamber has to be submitted to
ringer solution is shown in Fig. 5. A significant relation of sur- short pulses, so that only a small portion of the applied potential
vivor count to specific energy input was observed. Increasing builds up across the two double layer capacitors. Dependent on
the initial treatment temperature or the electric field strength the electrochemical properties, mainly the double layer capacity
leads to a further improvement of treatment efficiency. The crit- of its material, an electrode can withstand a pulse with a cer-
ical electric field strength for E. coli in ringer solution is in tain current density and an impulse length without significant
the range of 15 kV cm−1 , but in a previous study, modeling the damage. Stainless steel has a very low double layer capacity
impact of field strength based on inactivation kinetics of E. coli (35 F cm−2 ) compared to graphite (260 F cm−2 ), resulting in
in apple juice it was found that increasing field strength above a maximum pulse width avoiding electrochemical reactions as
this value is improving treatment efficiency [29]. Raising the low as 0.5 s with current densities of 200 A cm−2 [32], depen-
electric field strength from 16 to 20 kV cm−1 at a treatment tem- dent on energy per single pulse. Replacing the high voltage
perature of 35 ◦ C increased the microbial inactivation from 2 to anode made of stainless steel with a graphite electrode the inac-
3.3 log cycles at an energy input of 80 kJ kg−1 . In contrast to tivation study was repeated. In Fig. 6 a comparison between
the external threshold field strength the existence of a minimum inactivation with steel and graphite electrode at an electric field
energy level required to induce electroporation is rarely reported, strength of 16 kV cm−1 is shown at different initial treatment
although some fundamental works on the mechanisms of elec- temperatures, indicating a significant increase in treatment effi-
troporation consider alterations in free enthalpy which coincide ciency when using graphite. This finding might be caused by
with perturbations on the molecular level as a prerequisite of the an improved homogeneity of the electric field distribution in
membrane breakdown [30]. Statistically fluctuating membrane the treatment zone. Due to the higher double layer capacity of
defects, which naturally occur even in absence of PEF, deter- graphite the occurrence of Faradaic reactions at the electrode sur-
mine the membrane resistance and the critical transmembrane face might be reduced [37], possibly resulting in less electrolysis
voltage. and bubble formation. Gas bubbles, formed by electrolysis, cav-
In the same way the temperature level at which the PEF ity effects or release of dissolved gasses caused by heating have
treatment is performed can strongly reduce the stability of the a lower dielectric breakdown strength than the liquid media,
membrane against dielectric breakdown induced by external their presence will lead to perturbations of the electric field
electrical fields. This is mainly due to the transition from the distribution. The lower dielectric permittivity of air causes a con-
crystalline to the gel-like state [31] which enables a higher fluc- centration of potential within the bubbles increasing the chance
tuation rate of the defects within the membrane. for a dielectric breakdown and arcing [26]. Modeling the electric
7. S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546 543
cation of short pulses to avoid electrochemical reactions and
electrode erosion has been investigated by Morren et al. [34].
Local discharges and dielectric breakdown as well as perturba-
tions of electric field homogeneity due to the presence of bubbles
presumably might have caused the lower microbial inactivation
in case of the stainless steel anode. Arcing, as often observed in
presence of a big single air bubble in the treatment chamber was
not observed in both cases. Usage of graphite increased inactiva-
tion of E. coli more than 1 log cycle at many treatment intensities
as for example at 45 ◦ C and 60 kJ kg−1 . Combining applica-
tion of electrodes with low amount of electrochemical reactions
and pressurizing the treatment system to inhibit bubble forma-
tion as well as the application of higher electric field strengths
should lead to further improvements in treatment efficiency.
Bubbles should be avoided by degassing the treatment media
before treatment, processing under pressure particularly in case
of sparkling products and avoiding electrochemical effects at the
electrode/media interface.
Fig. 6. Comparison of inactivation of E. coli in ringer solution at a field strength 4.5. Inactivation of different microbial strains
of 16 kV cm−1 at different initial treatment temperatures with graphite (- - -) or
steel (– – –) anode. The flow rate was 5 kg h−1 , results are means based on data The inactivation of four microbial strains is plotted in Fig. 7
from two experiments, standard deviations are shown by error bars, the initial
count number was 1.58 × 107 CFU ml−1 .
dependent on specific energy input, emphasizing the differences
in PEF resistance between different microorganisms. It can be
seen that consistent to the mathematic modeling (see Fig. 1) the
field distribution with bubbles present in the treatment chamber smallest organism, L. innocua has a higher resistivity than organ-
it was shown that the field strength in the boundary region of isms with higher cell size like E. coli or B. megaterium. Above
a bubble is very low, possibly leading to under-processing, in that the cell membrane constitution has an important influence
particular between several bubbles. By using an electrode mate- on the stability of the membrane. The tendency that gram pos-
rial with higher double layer capacity like graphite electrolytic itive bacteria are more resistant than gram negative species has
effects should be reduced. Under-treatment in boundary regions frequently been reported [2,36,38,39].
of bubbles can be avoided, resulting in higher microbial inactiva- Whereas L. innocua showed a close to linear relation between
tion. Investigating the treated media at the outlet of the chamber energy input and inactivation rate, for E. coli a sigmoid curve
an reduced amount of bubbles when using graphite instead of a has been obtained. This curve shape is contrary to our earlier
steel anode could be confirmed, but still small bubbles ( 1 mm) studies conducted with parallel electrodes and a field strength
were found, which might also result from oversaturation of air in the range of 30–40 kV cm−1 [29]. In this study, using a treat-
due to heating by energy dissipation into the media. The appli- ment chamber with co-linear electrode configuration with a gap
Fig. 7. Inactivation of E. coli, Listeria innocua, Saccharomyces cerevisae and Bacillus megaterium in ringer solution with an electrical conductivity of 1.25 mS cm−1
after PEF treatment with graphite anode and a field strength of 16 kV cm−1 at different initial temperatures. The flow rate was 5 kg h−1 , results are means based
on two experiments, standard deviations are shown by error bars. The initial count number was 1.58 × 107 for E. coli, 7.9 × 106 for L. innocua, 1.6 × 107 for S.
cerevisae and 2.1 × 107 for B. megaterium. To avoid temperature increase above 70 ◦ C the maximum energy input was set to 50, 100 and 120 kJ kg−1 for initial
treatment temperatures of 35, 45 and 55 ◦ C, respectively.
8. 544 S. Toepfl et al. / Chemical Engineering and Processing 46 (2007) 537–546
of 6 mm the maximum electric field strength was limited to
20 kV cm−1 . An inhomogeneous distribution of the treatment
intensity in the co-linear treatment chamber may have caused
this effect, which was found at low treatment intensities only if
low impulse frequencies were applied. This may possibly result
in under-processing of volume elements with a short residence
time in the treatment zone. For inactivation of B. megaterium
and S. cerevisiae a very low input of specific energy in the range
of 10 and 30 kJ kg−1 is required for a 5 log cycle reduction at
55 ◦ C initial treatment temperature. Further increase of the spe-
cific energy input led, due to synergetic effects of PEF and heat,
to an inactivation below detection limit for these PEF sensi-
tive organisms. Temperatures higher than ambient and repetitive
pulsing may have led to a reduction of the required transmem-
brane potential below 1 V, resulting in a higher inactivation of Fig. 8. Enthalpy diagram of a suggested PEF treatment system for apple juice
L. innocua than predicted by Eq. (1) (see Fig. 1). An important with an initial temperature of 55 ◦ C and a specific energy input of 40 kJ kg−1 .
task will be the selection of resistant target strains to evaluate As specific heat capacity 3.8 kJ kg−1 K−1 was used, the heat loss in the heat
process efficacy, as also variations of PEF sensitivity among dif- exchanger was estimated for 5%.
ferent strains of L. monocytogenes have been described [40]. It
has to be taken into account that only vegetative cells are affected An important advantage of operating at elevated initial treat-
by a PEF treatment, whereas ascospores [41] and in particular ment temperature is the potential to recover the electrical energy
endospores show resistance [1]. dissipated into the product after treatment, as there is a need to
preheat the media. An enthalpy diagram of a suggested PEF
4.6. Thermal load and enthalpy balance of a PEF treatment treatment is shown in Fig. 8. Assuming a heat loss of 5% for
heat recovery, the process can be operated by the input of the
An initial treatment temperature of 55 ◦ C may lead to the electrical energy of 40 kJ kg−1 only, avoiding the necessity of
presumption that the advantage of PEF application, to provide a additional energy for preheating or cooling of the product after
mild and non-thermal preservation process is lost. Investigating treatment. When operating at ambient temperature there is no
the temperature-time-profile of a PEF treatment at an elevated potential to recover the dissipated energy as there is no need
treatment temperature of 55 ◦ C using synergetic effects of mild for preheating the media, and if the process shall be conducted
heat to reduce the required input of electrical energy showed that under close to isothermal conditions a huge amount of additional
compared to a conventional high temperature short time treat- energy is required for cooling.
ment the thermal load of the product is strongly reduced [29].
Dependent on the specific energy input and the specific heat 5. Conclusions
capacity of the medium the PEF treatment will cause a temper-
ature increase. For example for a pasteurization of apple juice The potential of PEF application for microbial inactivation in
with a heat capacity of 3.8 kJ kg−1 K−1 and an energy input of liquid food to increase shelf life was shown. A combination of
40 kJ kg−1 a temperature increase of 11 ◦ C is obtained, leading to PEF and mild heat provides the possibility for a gentle process
a process with a maximum temperature of 66 ◦ C for a very short to increase product shelf life with a low maximum temperature
residence time in range of seconds. A calculation of the C and and short residence times, resulting in a drastic reduction of the
the PU value, key benchmarks for the over all thermal load of the thermal load. Above that the specific energy input required for a
product during heat treatment [23,24] showed that a PEF treat- given inactivation is reduced by synergetic effects of temperature
ment with a specific energy input of 40 kJ kg−1 , a field strength of on microbial inactivation by PEF. When using an elevated initial
16 kV cm−1 and a initial treatment temperature of 55 ◦ C caused treatment temperature the dissipated electrical energy, causing
a 5 log cycle inactivation of E. coli in apple juice with very low a temperature increase of the product, can be recovered in a heat
Cook and PU values of 6.5 and 2.23 × 10−3 , respectively. A PEF exchanger to preheat the untreated media resulting in a dras-
treatment at elevated treatment temperature could therefore be tic reduction in costs of operation. The design of a treatment
an interesting, gentle alternative to increase shelf life of a high chamber with a homogenous electric field distribution, ensur-
acid product as for example fruit juice. A conventional HTST ing a highly uniform and sufficient treatment for all volume
treatment produces a shelf stable product with Cook and PU elements and the selection of an electrode material preventing
values of 6 × 102 and 0.45. Increasing the maximum electrical electrochemical reactions leads to a further increase of treat-
field strength of the impulse generating system would provide a ment efficiency. Modeling of the electric field distribution in
potential to achieve higher inactivation with similar energy input the treatment zone, the impact of particles or agglomerations
and thermal load [29]. Development of impulse generating sys- and the size and orientation of microbial cells on their sensitiv-
tems to achieve high electric field strength to operate with a gap ity against PEF provides a tool to choose appropriate process
allowing high flow rate capability and adequate, high pulse rep- parameters and therefore to ensure product safety. Further work
etition rate will be a crucial towards an industrial exploitation. will be necessary for choice of suitable electrode geometry and
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