This study aims to genotype plankton found in the guts of common Egyptian marine fish and their fry. Researchers will extract metagenomic DNA from the gut contents of wild mullet to obtain a complete phylogenetic profile of their gut microbiome. They will use PCR and denaturing gradient gel electrophoresis (DGGE) to amplify and analyze small subunit rRNA genes from the bacteria, archaea, and eukaryotes present. Sequencing the rRNA gene bands will allow construction of phylogenetic trees to identify the microbial species in the mullet guts. This genotyping technique provides a more complete picture of the wild mullet gut microbiome compared to traditional microscopy methods.
Adhesive proteins are industrially important proteins which were isolated and from the mussels and they were produced using conventional methods such as natural extraction. But now this protein is produced using genetic engineering technologies. It has wide applications in various arena.
Adhesive proteins are industrially important proteins which were isolated and from the mussels and they were produced using conventional methods such as natural extraction. But now this protein is produced using genetic engineering technologies. It has wide applications in various arena.
PPT in Biotechnology
Biotechnology provides powerful tools for the sustainable development of aquaculture, fisheries, as well as the food industry. Increased public demand for seafood and decreasing natural marine habitats have encouraged scientists to study ways that biotechnology can increase the production of marine food products, and making aquaculture as a growing field of animal research. Biotechnology allows scientists to identify and combine traits in fish and shellfish to increase productivity and improve quality. Scientists are investigating genes that will increase production of natural fish growth factors as well as the natural defense compounds marine organisms use to fight microbial infections. Modern biotechnology is already making important contributions and poses significant challenges to aquaculture and fisheries development. It perceives that modern biotechnologies should be used as adjuncts to and not as substitutes for conventional technologies in solving problems, and that their application should be need-driven rather than technology-driven.
The use of modern biotechnology to enhance production of aquatic species holds great potential not only to meet demand but also to improve aquaculture. Genetic modification and biotechnology also holds tremendous potential to improve the quality and quantity of fish reared in aquaculture. There is a growing demand for aquaculture; biotechnology can help to meet this demand. As with all biotech-enhanced foods, aquaculture will be strictly regulated before approved for market. Biotech aquaculture also offers environmental benefits. When appropriately integrated with other technologies for the production of food, agricultural products and services, biotechnology can be of significant assistance in meeting the needs of an expanding and increasingly urbanized population in the next millennium. Successful development and application of biotechnology are possible only when a broad research and knowledge base in the biology, variation, breeding, agronomy, physiology, pathology, biochemistry and genetics of the manipulated organism exists. Benefits offered by the new technologies cannot be fulfilled without a continued commitment to basic research. Biotechnological programmes must be fully integrated into a research background and cannot be taken out of context if they are to succeed.
Mayekar et al., 2021
INTRODUCTION
DEFINITION
HISTORY
TRANSGENIC FISH
METHODS OF GENE TRANSFER
HOW TO MAKE TRANSGENIC FISH
EXAMPLES
APPLICATIONS
TRANSGENIC BIRD
PRODUCTION METHOD
APPLICATIONS
CONCLUSION
REFRENCES
PPT in Biotechnology
Biotechnology provides powerful tools for the sustainable development of aquaculture, fisheries, as well as the food industry. Increased public demand for seafood and decreasing natural marine habitats have encouraged scientists to study ways that biotechnology can increase the production of marine food products, and making aquaculture as a growing field of animal research. Biotechnology allows scientists to identify and combine traits in fish and shellfish to increase productivity and improve quality. Scientists are investigating genes that will increase production of natural fish growth factors as well as the natural defense compounds marine organisms use to fight microbial infections. Modern biotechnology is already making important contributions and poses significant challenges to aquaculture and fisheries development. It perceives that modern biotechnologies should be used as adjuncts to and not as substitutes for conventional technologies in solving problems, and that their application should be need-driven rather than technology-driven.
The use of modern biotechnology to enhance production of aquatic species holds great potential not only to meet demand but also to improve aquaculture. Genetic modification and biotechnology also holds tremendous potential to improve the quality and quantity of fish reared in aquaculture. There is a growing demand for aquaculture; biotechnology can help to meet this demand. As with all biotech-enhanced foods, aquaculture will be strictly regulated before approved for market. Biotech aquaculture also offers environmental benefits. When appropriately integrated with other technologies for the production of food, agricultural products and services, biotechnology can be of significant assistance in meeting the needs of an expanding and increasingly urbanized population in the next millennium. Successful development and application of biotechnology are possible only when a broad research and knowledge base in the biology, variation, breeding, agronomy, physiology, pathology, biochemistry and genetics of the manipulated organism exists. Benefits offered by the new technologies cannot be fulfilled without a continued commitment to basic research. Biotechnological programmes must be fully integrated into a research background and cannot be taken out of context if they are to succeed.
Mayekar et al., 2021
INTRODUCTION
DEFINITION
HISTORY
TRANSGENIC FISH
METHODS OF GENE TRANSFER
HOW TO MAKE TRANSGENIC FISH
EXAMPLES
APPLICATIONS
TRANSGENIC BIRD
PRODUCTION METHOD
APPLICATIONS
CONCLUSION
REFRENCES
A short introductory presentation on Yeast Bioinformatics, focussing on the Yeast Genome and its future applications. Intended as a starting material to learn more about Saccharomyces Genomics.
1. National institute of oceanography
Laboratory of genetics
Genotyping of Plankton Useful for
Feeding Common Egyptian Marine
Fishes and their Fries
األسماك معظم لتغذية المفيدة الهائمات لبعض الوراثية البصمة
وزريعتها المصرية البحرية.
Presentation
By
Name: Hany Tolba
2. Supervisors
Prof. Dr Mohamed Abd Elsalam Rashed
professor of Genetics, Faculty of Agriculture
Ain Shams university
Prof. Dr Mahmod Ahmed Abd Elhafez Sallam
professor of Genetics, Faculty of Agriculture
Ain Shams university
Dr Hosam Elsaied
Assistant professor of Genetics, NIOF
4. What are marine common fishes in Egypt ?
Of course
Mugilidae
- Mugil cephalus
- Liza ramada
- Liza aurata
Mugil cephalus Liza aurataLiza ramada
5. The idea of the study :Problems in feeding
Mullet fish ?
Traditional methodology
No complete profile for Wild Mullet gut Microbiome
Disadvantages
Most of Microbiome
can not be detected
undre microscope ,
because , they are
partially digested .
Mullet gut
Genomics
Technique
Looking
microscopy
6. Main Targets of the study
1- Obtain a complete Phylogenetic for
Wild type gut Microbiom
2- Construct Life Gene Atlas, for Plankton
Feeding Wild Mullet.
7. What is the Microbime Types Expected
to be Found in Gut of Mullet?
Bacteria Archaea Eukaryote
red slime algae
( cyanobacteria)
Blue Green algae
( cyanobacteria)
Crab LarvaCopepod
Crab Larva
Oerbacteriën
(Methanopyrus
kandleri)
8. 1- Sampling of wild mullet and evacuation of gut
content
2- Metagenomic DNA Extraction of gut microbiome
3- PCR amplification of small subunit rRNA gene as
the finger print of gut microbial species
4- Denaturant Gradient Gel Electrophoresis, DGGE
5- Analysis of DGGE product by sequencing
6- Construct phylogenetic trees of recorded species
based on sequenced rRNA genes
Plan of Work: for Detection of
Microbiome in the gut of Mullet.
9. 1- Sampling of wild mullet and
evacuation of gut content
Bardwell LakeManzala Lake
12. 2- Metagenomic DNA Extraction of gut microbiome
This is an Example for DNA Extraction of Gut
Content from 5 Fish Individual .
Hosam Elsaied research group
13. 3-PCR Using rRNA gene
Specific Primers
Bacteria Archaea Eukaryote
16 S rRNA gene
Types of amplified r RNA gene
18 S r RNA gene
18. Example of use DGGE Technique for eukaryote sample
Common plankton rRNA gene band occurred
in both samples
a b
100 bp
ladder
100 bp
Polyacrylamide DGGE gel
Agarose gel
Applied to polyacrylamide DGGE as duplicate samples
Analyzed by sequence
PCR using 18S rRNA gene of
Metagenomic DNA ( a , b )
Hosam Elsaied research group
20. PCR Using rRNA gene(16S,18S)
by Specific Primers
Environmental sample
(mullet gut content)
Metagenomic DNA Extraction
DGGE
sequencing
phylogenetic
Summary