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1. November 16, 2014 1
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
International Conference on Advances
in Healthcare & Life Sciences
(Singapore) 8 – 9 November 2014

2. INFLUENCE OF Weisella paramesenteroides ISOLATE
“DADIH”TO THE BOWEL FREQUENCE, SECRETORY
IMMUNOGLOBULIN A LEVEL AND HEIGHT OF ILEUM
VILLI OF THE MICE EPEC INDUCED DIARRHEA
November 16, 2014 2
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
ANDALAS UNIVERSITY
PADANG, WEST SUMATERA
INDONESIA
Laboratory of Biotechnology / Technology of
Product Husbandry, Faculty of Animal Science,
Andalas University, Padang, West Sumatera,
Indonesia
Email : purwati17@yahoo.co .id

3. INTRODUCTION
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
3

10. Benefit of Research
researchers interested in studying the
effect of these bacteria on diarrhea
particularly levels of SigA and ileum
villi.
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
10

11. MATERIAL
AND
METODE
RESEARCH
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
11

13. • Experimental animals were randomized into two
factors:
• The first factor (A) is the dose of probiotic
administration, consist of a negative control: only
given standard feed and water (A1), positive control:
EPEC administered at a dose of 108
cfu/g (A2), W.
paramesenteroides dose of 2 x 108
cfu/g (A3), W.
paramesenteroides dose of 2 x 109
cfu/g (A4), W.
paramesenteroides with a dose of 2 x 1010
cfu/g.
• The second factor (B) is the duration of observation
(hour), which consist of 0 hour (B1), 12 hours (B2), 24
hours (B3), 36 hours (B4)

14. Observed variables :
1. Examination of bowel frequency,
2. levels of SIgA and
3. height of ileum villi
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
14

15. November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
15
RESULTS AND
DISCUSSION
RESULTS AND
DISCUSSION

16. Tabel 1. Mean bowel frequency(times) of mice based on
doses and duration of observation
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
16
A Factor
(doses)
B Factor (duration of observation)
Total Mean SDB1= 0
hour
B2=12
hours
B3 = 24
Hours
B4 = 36
hours
A1=K (-) 4,00g
5,00g
4,50g
5,00g
18,50 4,63 1.52
A2=K (+)
5,00g
50,50a
41,00b
37,00c
133,50 33,38
4.09
A3=2x108
5,50g
48,50a
16,50e
11,00f
81,50 20,38
3.19
A4=2x109
5,00g
30,50d
20,00e
16,00e
71,50 17,88
2.99
A5=2x1010
5,50g
28,50d
12,50f
11,00f
57,50 14,38
2.68
Total 25,00 163,00 94,50 80,00 362,50 90,63
Mean 5,00 32,60 18,90 16,00 72,50 18,13
Note : different small letter superscripted in different columns and rows has highly significant difference (P<0.01).

17. Table 2. MeanSIgA (µg/ml) in feces based on doses and
duration of observation
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
17
Factor A
(dose)
Factor B (duration of observation)
Total Mean SDB1 = 0
hours
B2 = 12
hours
B3 = 24
hours
B4 = 36
hours
A1 = K(-) 1.82e
1.80e
1.82e
1.81e
7.26 1.82 0.95
A2 = K(+) 1.83e
1.57h
1.60g
1.66f
6.66 1.66 0.91
A3 = 2x108
1.84e
1.53h
2.27b
2.63a
8.59 2.15 1.04
A4 = 2x109
1.83e
1.59h
2.13c
2.26b
7.80 1.95 0.99
A5 =
2x1010
1.82e
1.56h
1.96d
2.17c
7.52 1.88
0.97
Total 9.14 8.05 10.24 10.40 37.83 9.46
Mean 1.83 1.61 2.05 2.08 7.57 1.89
Note : different small letter superscripted in different columns and rows has highly significant difference (P<0.01).

18. Table 4. Mean of height of ileum villi (µm) based on
doses and duration of observation
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
18
Factor A
(Dose)
Factor B (duration of observation)
Total Mean SD
B1 = 0
hours
B2 = 12 hours B3 = 24 hours B4 = 36
hours
A1 = K(-) 111.21g
111.01g
115.49g
116.66g
454.37 113.59 7.54
A2 = K(+) 111.86g
54.33k
91.38h
116.73g
374.30 93.58 6.84
A3 = 2x108
111.17g
66.72j
156.42d
187.26a
477.10 119.28 8.07
A4 = 2x109
112.32g
72.68i
129.19f
164.06c
535.72 133.93 7.73
A5 = 2x1010
114.99g
52.08k
150.54e
174.80b
482.41 120.60 7.85
Total 564.55 356.82 643.02 759.51 2323.90 580.98
Mean 112.91 71.36 128.60 151.90 464.78 116.20
Note : different small letter superscripted at different columns and rows showed highly different significance (P<0.01).

19. Conclusion
Giving W.paramesenteroides dosing
2x108
cfu/g after 24 hours significantly
decreased the frequency of bowel, increased
levels of SIgA and improving ileum villi in mice
EPEC-induced diarrhea.
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
19

20. Acknowledgements
This research was supported by Laboratory of
Biotechnology / Technology of Product
Husbandry, Faculty of Animal Science, Andalas
University, Padang. Indonesia and Hibah
Pascasarjana Nomor : 004/UN.16/PL/MT-HB-
PC/2012 Date 24 January 2012 Prof. Drh. Hj.
Endang Purwati, MS, Ph.D.
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
20

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November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
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24. THANK YOU
November 16, 2014
Prof. Drh. Hj. ENDANG PURWATI R N, MS.,
Ph.D
24