The document summarizes the author's major contributions in two areas: 1. Drug target discovery and mechanistic study through developing platforms for metabolic fluxomics analysis, LC-MS/MS based metabolomic and proteomic studies to screen and validate drug targets. 2. Drug development including in vitro enzyme assays and cell-based assays for compound screening, and LC-MS/MS studies in disease models to prove target engagement and pathway analysis. The author has technical expertise in spectroscopy, enzyme studies, protein expression and purification, chromatography, metabolomics, and experience in the pharmaceutical industry and drug development.