Analysis of pesticides in food using both LC- and GC-MS/MS, with data and description of Atmospheric Pressure GC, available on the same system as UPLC-MS/MS with rapid changeover.
Picarro - A Revolution in Food Safety and Food Fraud DetectionPicarro
Picarro makes the worlds highest performing and easiest to use gas analyzers. Picarro analyzers are revolutionizing the way the farmers, grocers, distributors, and regulators trace where food comes from, identify point of origin, and screen for food fraud and adulteration. Visibility into the authenticity and origin of the food we eat is becoming increasingly important in an era of globalized food distribution.
Research Inventy : International Journal of Engineering and Scienceresearchinventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Analysis of pesticides in food using both LC- and GC-MS/MS, with data and description of Atmospheric Pressure GC, available on the same system as UPLC-MS/MS with rapid changeover.
Picarro - A Revolution in Food Safety and Food Fraud DetectionPicarro
Picarro makes the worlds highest performing and easiest to use gas analyzers. Picarro analyzers are revolutionizing the way the farmers, grocers, distributors, and regulators trace where food comes from, identify point of origin, and screen for food fraud and adulteration. Visibility into the authenticity and origin of the food we eat is becoming increasingly important in an era of globalized food distribution.
Research Inventy : International Journal of Engineering and Scienceresearchinventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
Endoscope surveillance - comparison of sampling techniquesOneLife SA
Objective : To compare different techniques of endoscope sampling to assess residual bacterial contamination.
Design : Diagnostic study
Setting : The endoscopy unit of a 1.100-bed university teaching hospital performing approximately 13 000 endoscopic procedures annually.
Lignocellulose Biomass- Hydrolysis & Fermentation Lab Protocols
YOU AGREE TO INDEMNIFY BiorefineryEPCTM , AND ITS AFFILIATES, OFFICERS, AGENTS, AND EMPLOYEES AGAINST ANY CLAIM OR DEMAND, INCLUDING REASONABLE ATTORNEYS' FEES, RELATED TO YOUR USE, RELIANCE, OR ADOPTION OF THE DATA FOR ANY PURPOSE WHATSOEVER. THE DATA ARE PROVIDED BY BiorefineryEPCTM "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESSLY DISCLAIMED. IN NO EVENT SHALL BiorefineryEPCTM BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER, INCLUDING BUT NOT LIMITED TO CLAIMS ASSOCIATED WITH THE LOSS OF DATA OR PROFITS, WHICH MAY RESULT FROM ANY ACTION IN CONTRACT, NEGLIGENCE OR OTHER TORTIOUS CLAIM THAT ARISES OUT OF OR IN CONNECTION WITH THE USE OR PERFORMANCE OF THE DATA.
Resveratrol: A Novel Antiseptic for Oral Hygiene Productsjuliabarquin
My study researched the inhibitory properties of resveratrol on the bacterial growth of Streptococcus Mutans. If you have any questions about my research please feel free to contact me via email at julia.barquin@icloud.com. All Rights Reserved.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Endoscope surveillance - comparison of sampling techniquesOneLife SA
Objective : To compare different techniques of endoscope sampling to assess residual bacterial contamination.
Design : Diagnostic study
Setting : The endoscopy unit of a 1.100-bed university teaching hospital performing approximately 13 000 endoscopic procedures annually.
Lignocellulose Biomass- Hydrolysis & Fermentation Lab Protocols
YOU AGREE TO INDEMNIFY BiorefineryEPCTM , AND ITS AFFILIATES, OFFICERS, AGENTS, AND EMPLOYEES AGAINST ANY CLAIM OR DEMAND, INCLUDING REASONABLE ATTORNEYS' FEES, RELATED TO YOUR USE, RELIANCE, OR ADOPTION OF THE DATA FOR ANY PURPOSE WHATSOEVER. THE DATA ARE PROVIDED BY BiorefineryEPCTM "AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE EXPRESSLY DISCLAIMED. IN NO EVENT SHALL BiorefineryEPCTM BE LIABLE FOR ANY SPECIAL, INDIRECT OR CONSEQUENTIAL DAMAGES OR ANY DAMAGES WHATSOEVER, INCLUDING BUT NOT LIMITED TO CLAIMS ASSOCIATED WITH THE LOSS OF DATA OR PROFITS, WHICH MAY RESULT FROM ANY ACTION IN CONTRACT, NEGLIGENCE OR OTHER TORTIOUS CLAIM THAT ARISES OUT OF OR IN CONNECTION WITH THE USE OR PERFORMANCE OF THE DATA.
Resveratrol: A Novel Antiseptic for Oral Hygiene Productsjuliabarquin
My study researched the inhibitory properties of resveratrol on the bacterial growth of Streptococcus Mutans. If you have any questions about my research please feel free to contact me via email at julia.barquin@icloud.com. All Rights Reserved.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
In silico drugs analogue design: novobiocin analogues.pptx
Ferreira1997
1. This article was downloaded by: [University of West Florida]
On: 03 October 2014, At: 06:11
Publisher: Taylor & Francis
Informa Ltd Registered in England and Wales Registered Number: 1072954
Registered office: Mortimer House, 37-41 Mortimer Street, London W1T 3JH,
UK
Journal of Liquid
Chromatography & Related
Technologies
Publication details, including instructions for
authors and subscription information:
http://www.tandfonline.com/loi/ljlc20
Development of An HPLC-UV
Method for Determination of
Taurine in Infant Formulae and
Breast Milk
I. M. P. L. V. O. Ferreira
a
, M. V. Nunes
a
, E. Mendes
a
, F. Remião
b
& M. A. Ferreira
a
a
CEQUP
b
Laboratório de Bromatologia Laboratório de
Toxicologia Faculdade de Farmácia, Universidade do
Porto Rua Aníbal Cunha , 164 4050, Porto, Portugal
Published online: 23 Sep 2006.
To cite this article: I. M. P. L. V. O. Ferreira , M. V. Nunes , E. Mendes , F. Remião
& M. A. Ferreira (1997) Development of An HPLC-UV Method for Determination of
Taurine in Infant Formulae and Breast Milk, Journal of Liquid Chromatography &
Related Technologies, 20:8, 1269-1278, DOI: 10.1080/10826079708010975
To link to this article: http://dx.doi.org/10.1080/10826079708010975
PLEASE SCROLL DOWN FOR ARTICLE
Taylor & Francis makes every effort to ensure the accuracy of all the
information (the “Content”) contained in the publications on our platform.
However, Taylor & Francis, our agents, and our licensors make no
representations or warranties whatsoever as to the accuracy, completeness,
or suitability for any purpose of the Content. Any opinions and views
expressed in this publication are the opinions and views of the authors, and
are not the views of or endorsed by Taylor & Francis. The accuracy of the
2. Content should not be relied upon and should be independently verified with
primary sources of information. Taylor and Francis shall not be liable for any
losses, actions, claims, proceedings, demands, costs, expenses, damages,
and other liabilities whatsoever or howsoever caused arising directly or
indirectly in connection with, in relation to or arising out of the use of the
Content.
This article may be used for research, teaching, and private study purposes.
Any substantial or systematic reproduction, redistribution, reselling, loan,
sub-licensing, systematic supply, or distribution in any form to anyone is
expressly forbidden. Terms & Conditions of access and use can be found at
http://www.tandfonline.com/page/terms-and-conditions
Downloadedby[UniversityofWestFlorida]at06:1103October2014
3. J. LIQ. CHROM. & REL. TECHNOL., 20(8), 1269-1278 (1997)
DEVELOPMENT OF AN HPLC-UV METHOD
FOR DETERMINATION OF TAURINE IN INFANT
FORMULAE AND BREAST MILK
I. M. P.L. V. 0.Ferreira,t M. V. Nunes, E. Mendes,
F. Remiilo,I M. A. Ferreirat
~CEQUP
tLaboratoriode Bromatologia
Laboratbriode Toxicologia
Faculdade de Farmacia, Universidade do Porto
Rua Anibal Cunha 164
4050 Porto, Portugal
ABSTRACT
A rapid and accurate hgh performance liquid chromato-
graphic (HPLC)procedurewas proposed for routine and selective
determination of taurine in infant formulas and breast milk. The
sample preparation was simple, involving protein removal and
filtration operations. Afterwards, taurine was derivatised with 0-
phthalaldehyde/2-mercaptoethanol prior to injection onto a
reversed phase column CI8 (SloODS2). Isocratic elution was
carried out using 0.05 M phosphate buffer pH 5.3/ methanol
(60:40) mixture. The effluentwas monitored by a UV detector at
350 nm. A linear relationship was found between peak area and
a concentration range of 1-70 pg/mL. The detection limit was
0.3 pg/mL. Effective separation and quantification was achieved
in under six minutes. No interference of other amino acids was
observed.
1269
Copyright 8 1997by Marcel Dekker. Inc.
Downloadedby[UniversityofWestFlorida]at06:1103October2014
4. 1270 FERREIRA ET AL.
Extensive validation of the proposed method was carried out
both by the standard additions method and by comparison with a
fluorimetric method of known accuracy. The precision was better
than 1.4%.
INTRODUCTION
Taurine. 2-aminoethanesulphonic acid. is a sulphur-containing p-amino
acid that is not incorporated into proteins. However, it takes part in
biochemical reactions of major importance, such as conjugation with bile acids
to form bile salts essential for fat absorption, cell membrane stabilization,
antioxidation. detoxification. osmoregulation, neuromodulation, and brain and
retinal development.’
Considerable evidence has accumulated that neonates and infants, who
have a very limited ability to synthesizetaurine, are especially prone to develop
taurine deficiency and that they depend on an external taurine supply.’
Taurine concentrations in milk are very variable, depending on species.
Wlule breast milk is an excellent source of taurine in the developing infant.
cow’s milk has a very low taurine content. Nowadays the supplementation of
infant formulae with taurine in concentrations similar to those found in human
milk is therefore re~ommended.~.‘
Many methods for measuring taurine in several matrices viz. biological
samples, such as tissue homogenates, urine, and serum have been published,
including gas chromatography’ and HPLC analysis of diferent amino acid
derivatives.6-” One of the most sensitive and commonly used derivatising
agent techniques is the use of o-phthaldehyde / mercaptoethanol, which,
originates a fluorescent adduct that enables the fluorimetric determination of
taurine.6*1’
Other HPLC fluorimetric determinations in similar matrices include
derivatising reaction with hamine*and fluore~camine.~
It is also possible to use UV absorbance detection at 350 nm with different
derivatisation techniques. includmg o-phthaldehyde / mercaptoethanol’ and
dinitrofluorobenzene.‘~,’~Interestingly. research work developed by Chen at
aI.l2is distinguished from the rest since one of the matrices used was human
milk.
Downloadedby[UniversityofWestFlorida]at06:1103October2014
5. TAURINE IN W A N T FORMSJLAE AND BREAST MILK 1271
Some of these methods require time-consuming pre-treatment of the
samples. in order to eliminate interfering substances or complicated
derivatising treatment of the samplebefore assay.
This paper describesa rapid and accurate method for the determination of
taurine in infant formulae and breast milk. Although, the sample matrix is of
complex nature, the pre-treatment applied is simple and the method is suitable
for rapid routine assays of a large number of samples and uses a UV detector
which is much more common in control laboratories than the fluorimetric
detector.
MATERIALS
Apparatus
The chromatographic analysis was carried out in a Gilson, high
performance liquid Chromatograph (Gilson Medical Electronics, Villiers le
Bel, France) equipped with a type305 pump, a type 302 pump and a type 7125
Rheodyne Injector with a 20 p1 loop. A Gilson 118, variable long wave ultra
violet detector was also used.
The chromatographic separation was achieved with a Spherisorb Slo
ODs2Chromatographiccolumn, 10 pm. The integrator used was a Gilson 712
HPLC System Controller Software.
A Gilson 121 Spectrofluorometerwas used for detection of the fluorescent
adduct.
Reagents and Standards
Taurine and o-phthaldehydewere obtained from Sigma Chemical Co. L-
Amino acids kit was also from Sigma (Kit No. LAA-21). Sulphosalicylicacid,
boric acid, sodium hydroxide and mercaptoethanol,all p.a., were obtained from
Merck. Methanol (LiCrosolv)was Merck "gradient grade".
Water used for chromatography possessed a resistance greater than 15
Ma. and was filtered through a membrane of 0.45 pm porosity which was
subsequentlydegassed.
Downloadedby[UniversityofWestFlorida]at06:1103October2014
6. 1272 FERREIRA ET AL.
Sample Preparation
After homogenisation. 5.0 g of infant formulae were dissolved in 40 mL
of warm (40°C) water. 5.0 mL of the infant formulae solution or breast milk
were added to 5.0 mL of sulphosalicylic acid solution 0.2 M. mixed thorougly.
and allowed to stand for 10 min. All samples were filtered through W42 paper
and thereafter. through 0.2 pm filter paper.
The use of sulphosalicylic acid as a protein precipitating agent has been
shown to result in a higher YOrecovery of taurine.”
Denvatisation Procedure
Taurine was derivatised with o-phthalaldehyde (OPA, 40 mg, 0.8 mL
absolute ethanol) and 2-mercaptcethanol (4Opl) in 0.5 M borate buffer (10 mL,
3.1 g boric acid in 90 mL water. adjusted to pH 10.4with 5M NaOH. and made
up to 100 mL)6prior to injection onto a reversed phase column CIS(SloODS2).
The derivatizing solution was prepared daily and filtered through a 0.20
pm filter before use.
A 100 pL volume of standard or sample solution (after preparation) was
placed in an eppendorf and 100 pl of derivatizing reagent were added. The
derivatization reaction was allowed to proceed for exactly 1.5 min, at which
time an aliquot was injected into the HPLC.
Chromatography
The HPLC elution required a mixture of two solvents. Solvent A. 0.05 M
phosphate buffer pH 5.3. and solvent B. methanol. The two solvents were
filtered and degassedbefore use. Isocratic elution was carried out at a flow rate
of 1.5 mL/min, using 60% solvent A combined with 40 YOsolvent B. The
injection volume was 20 p1 and the chromatographic analysis was conducted at
ambient temperature.
Taurine concentration was determined at an absorbance of 350 nm. which
is the maximum wavelength on the excitation spectrum of OPA-derivatized
amino acids. Taurine peak was identified by coelution with a standard and by
comparison of the retention time.
Downloadedby[UniversityofWestFlorida]at06:1103October2014
7. TAURINE IN INFANT FORMULAE AND BREASTMILK 1273
Figure 1. Standard curves for taurine. Taurine was derivatised and chromatographed
by the method described in the text. Each point represents the average of triplicate
determinations. (a) Sensitivityof 0.06 AUFS, detection limite 1 p g / d . (b) Sensitivity
of 0.03AUFS, detection liniite 0.3 pg/mL.
RESULTS
Analytical Curve and Detection Limit
Under the assay conditions described, a linear relationship between the
concentrationof taurine and the UV absorbance at 350 nm was obtained. This
linearity was mantained over the concentration range 3-70 pg/mL with a
sensitivityof 0.06AUFS and 1-10 pg/mL with a sensitivity of 0.03 AUFS. The
detection limit value was calculated as the concentrationcorrespondingto three
times the standard deviation of the background noise and was 1 pg/mL and 0.3
pg/mL, respectively. The calibrationcurvesfor taurine are ilustratedin Fig. 1.
Downloadedby[UniversityofWestFlorida]at06:1103October2014
8. 1274 FERREIRAET AL.
....
Figure 2. Typical chromatogram obtained for taurine extracted from infant formulae.
Retention time of taurine 4.51.
Triplicate determinations were made on each of the calibration standards,
the peak area values (arbitrary units) that were plotted are average values. The
relative percent average deviationsof triplicates were less than 3 YOin all cases.
Validity of the Method
The repeatability of the derivatization procedure was examined. A
standard taurine solution was derivatized and chromatographed six times.
When reaction and chromatographic conditions were carefidly optimized, the
standard deviationwas 0.02 (n=6 and concentrationof taurine 20 pg/mL).
Downloadedby[UniversityofWestFlorida]at06:1103October2014
9. T A W IN INFANTFORMULAE AND BREASTMILK 1275
Table 1
Recovery of Taurine from Spiked Infant Formulae
&mL pg/mL Standard CV% Recovery
Added Found’ Deviation YO
2.00 2.03 M.05 2.46 101.5
Sample 1 4.00 3.92 M.08 2.04 98.0
6.00 5.82 M.09 1.54 97.0
2.00 1.99 34.03 1.51 99.5
Sample9 4.00 4.10 k0.07 1.71 102.5
6.00 5.73 34.06 1.05 95.5
“Meanvalue found for 3 assays for each studiedconcentrations.
The interference of other amino acids was examined nest. For this
purpose, different amino acids were injected after derivatization with OPA.
They included L-Alanine, L-Valine, L-Leucine, L-Isoleucine. L-Proline. L-
Phenylalanine,L- Tryptophan, L- Methionine, Glycine, L-Serine, L-Threonine,
L-Tyrosine, L-Asparagine. L-Glutamine. L-Aspartic acid. L-Glutamic acid. L-
Lysine, L-Arginine, L-Histidine. Different retention times were obtained for
each amino acid.
Fig. 2 shows a typical chromatograph of taurine extracted from infant
formulae. Peaks corresponding to several amino acids were detected in the
chromatograms obtained for infant formulae determinations, but as shown,
these peaks were well separated from those of taurine under the conditions
used. A similar behaviourwas observedwith regards to breast milk.
The precision of the analytical method was evaluated by measuring the
peak chromatographicarea 10times on the same sample. The relative standard
deviation (RSD) ranged between 0.7 and 1.4 YO(concentration of taurine in
infant formula 42.6 and 28.6 mg of taurineA00 g of infant formula.
respectively).
Recovery studies were carried out on two different samples of infant
formulae, sample 1 with high concentration of taurine, and sample 9 with very
low concentration of taurine. The samples were analysed in triplicate before
and after the addition of known amounts of taurine and they were spiked with
Downloadedby[UniversityofWestFlorida]at06:1103October2014
10. 1276 FERREIRA ET AL.
Table 2
Comparison Between Taurine Determined in Infant Formulae and Breast
Milk by HPLC-UV and a Comparison Method of Known Accuracy'
Taurine Determined (mg/100 g of Infant Formula)
Sample
No. HpLC-UV(y) Comparison method (x)
1
2
3
4
5
6
7
8
9
10
39.4 f0.7b
34.4 f 1.3b
28.6 f0.3b
39.0 f 0.9b
38.0 f 0.2b
44.7 f0.7b
32.7 *0.9b
30.2 f l.lb
1.O1 f 0.08"
3.99 f O.2lC
40.6 f 0.5
32.7 f0.2
27.7 *0.7
10.3k 0.5
38.8 f 0.6
43.1 +0.3
32.9 f0.1
31.0 f 0.9
0.80 ? 0.04
4.32 k0.11
Taurine Determined (mg/100 mL of Breast Milk)
11 4.13 f0.06b
12 4.25 kO.Ogb
13 2.28 f0.1lb
14 2.18 f0.09b
4.23 f 0.10
4.32 f0.05
2.43 f0.08
2.08 f0.12
aValuesare expressed as mean fstandard deviation of three determinations.
Sensitivity of 0.06 AUFS.
"Sensitivityof 0.03 AUFS.
Samples 1 to 10are from infant formulae, samples 11 to 14 are from breast
milk.
b
three different concentrations of taurine. The results are listed in Table 1. The
addition of taurine to the infant formulae was made before the protein removal.
Thus, this procedure proved the effectiveness of the extraction step and the
accuracy of the proposed method. Recovery values were between 95.5 and
102.5%. which confirm no interference effects due to matrix composition.
Downloadedby[UniversityofWestFlorida]at06:1103October2014
11. TAURINJ?IN INFANT FORMULAE AND BREAST MILK 1277
In order to validate the accuracy of the analytical method and because
there was no certified reference material available, not only the standard
addition method was made. but also a comparison with a method of known
accuracy." The results of these determinations of taurine in infant formulae
and breast milk are presented in Table 2. In both cases quantification was
based on the external standard method.
No significant differences between the results obtained with the present
method CY) and the comparison method (x)were obtained when determined by
ANOVA methodology. followed by Fisher's PLSD test. (Differences were
consideredsignificant for p < 0.01). Regression lines between the two methods
were y = 0.996 x + 0.108. (r = 0.997) and y = 1.01 x - 0.042. (r = 0.993) for
infant formulaeand breast milk. respectively.
CONCLUSION
An accurate and precise method to quantifytaurine in infant formulae and
breast milk is presented.
Despite the complexity of the matrix, the sample pre-treatment is simple
and this approach only requires a basic HPLC system without extra reagent
pumps, mixing manifolds, reaction coils, etc. which is an obvious advantage of
precolumn derivatisation relatively to post column derivatisation.' However. it
requires rigorous control of the OPA-reagent and reaction time in order to
obtain the high degreeof reproducibitily shown here.
The chromatographicrun time of 5 minutes is comparablewith the lowest
reported time for fluorimetric determinations'' and less than the run time
reported for UV determinations.'
ACKNOWLEDGEMENT
This work received financial support from Junta Nacional de Investigaqlo
Cientifica Project0 PBIC/C/TPR/ 2564/95.
REFERENCES
1. I. Zelikovic. R. W. Chesney. "Taurine", in New protectiveRoles for
Selected Nutrients. Alan R. Liss. Inc.. pag. 253-294. (1989).
Downloadedby[UniversityofWestFlorida]at06:1103October2014
12. 1278 FERREIRA ET AL.
2. G. E. Gaull. Pediatrics. 83.133442. (1989)
3. R. W. Chesney. Ped. Res.. 22. 755-759 (1987).
4. First Report of the Scientific Committee for Food related with the
caracteristics of the infant formulae based on cow's milk. Commission of
the European Communities. Luxemburg. 14th sene. EUR 8752 (1983).
5. A. A. Stampfli. 0.Ballevre, L. B. Fay. J. Chromatogr.. 617, 197-203
(1985).
6. B. R. Larsen. D. S. Grosso. S. Y. Chang. J. Chromatogr.Scienc.. 18, 233-
236 (1980).
7. T. Hirai. H. Ohyama. R. Kido. Anal. Biochem.. 163,339-342 (1987).
8. T. Yokoyama. T. Kinoshita. J. Chroaatogr.. 106. 212-218 (1991).
9. T. Sakai. T. Nagasawa. (1992) J. Chromatogr.. 114. 155-157 (1992).
10.N. Masuoka. K. Yao. M. Kinuta. J. Ohta, M. Wakimoto, T. Ubuka. J.
Chromatogr. B. 660. 31-35 (1994).
11. C. J. Waterfield. J. Chromatogr. B, 657. 37-45 (1991) .
12.Z. L. Chen, G. Xu. K. Specht. R. J. Yang, S. W. She, Anal. Chim. Acta,
296. 249-253 (1994) .
Received August 6, 1996
Accepted August 26. 1996
Manuscript 4217
Downloadedby[UniversityofWestFlorida]at06:1103October2014