Plants synthesise a huge variety of fatty acids although only a few are major and common constituents . Broadly speaking, long-chain fatty acids are synthesised de novo from small precursors ultimately derived from photosynthate.
Presentation on Fatty Acid Biosynthesis in cell (Creation of fatty acids from acetyl-CoA and NADPH through the action of enzymes called fatty acid synthases).
Biosynthesis of fatty acids & eicosanoidsMeghanasweetie1
This presentation provides the knowledge about Biosynthesis of Fatty acids & eicosanoids, Pathways, De novo fatty acid Synthesis, Advantages, Significance, Pharmacological Applications.
Plants synthesise a huge variety of fatty acids although only a few are major and common constituents . Broadly speaking, long-chain fatty acids are synthesised de novo from small precursors ultimately derived from photosynthate.
Presentation on Fatty Acid Biosynthesis in cell (Creation of fatty acids from acetyl-CoA and NADPH through the action of enzymes called fatty acid synthases).
Biosynthesis of fatty acids & eicosanoidsMeghanasweetie1
This presentation provides the knowledge about Biosynthesis of Fatty acids & eicosanoids, Pathways, De novo fatty acid Synthesis, Advantages, Significance, Pharmacological Applications.
1.FATTY ACID SYNTHESIS FOR MBBS, LABORATORY MEDICINEAND BDS.pptRajendra Dev Bhatt
Lipid metabolism is the processing of lipids for energy use, energy storage, and structural component (Cholesterol & lipoproteins) production. Lipids are digested by lipase enzymes in the GI tract (with the help of bile acids) and are absorbed directly through the cell membrane. Free fatty acids are then resynthesized into triacylglycerols (TAGs) in the enterocytes. Finally, lipid components are repackaged into chylomicrons and transported throughout the body for use or storage.
Lipid metabolism is the synthesis and degradation of lipids in cells, involving the breakdown and storage of fats for energy and the synthesis of structural and functional lipids, such as those involved in the construction of cell membranes. In animals, these fats are obtained from food and are synthesized by the liver. Lipogenesis is the process of synthesizing these fats. The majority of lipids found in the human body from ingesting food are triglycerides and cholesterol.[4] Other types of lipids found in the body are fatty acids and membrane lipids. Lipid metabolism is often considered as the digestion and absorption process of dietary fat; however, there are two sources of fats that organisms can use to obtain energy: from consumed dietary fats and from stored fat.[5] Vertebrates (including humans) use both sources of fat to produce energy for organs such as the heart to function. Since lipids are hydrophobic molecules, they need to be solubilized before their metabolism can begin. Lipid metabolism often begins with hydrolysis, which occurs with the help of various enzymes in the digestive system.Lipid metabolism also occurs in plants, though the processes differ in some ways when compared to animals.[8] The second step after the hydrolysis is the absorption of the fatty acids into the epithelial cells of the intestinal wall.[6] In the epithelial cells, fatty acids are packaged and transported to the rest of the body.[9]
Metabolic processes include lipid digestion, lipid absorption, lipid transportation, lipid storage, lipid catabolism, and lipid biosynthesis. Lipid catabolism is accomplished by a process known as beta oxidation which takes place in the mitochondria and peroxisome cell organelles.
Fatty acid synthesis:- Everything you need to knowGunveenkaur10
Fatty acid synthesis is a very important topic of biotechnology and biochemistry.
The following presentation includes everything from an introduction to fatty acids and de novo synthesis.
At the end are my social media accounts, please consider the following.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
1.FATTY ACID SYNTHESIS FOR MBBS, LABORATORY MEDICINEAND BDS.pptRajendra Dev Bhatt
Lipid metabolism is the processing of lipids for energy use, energy storage, and structural component (Cholesterol & lipoproteins) production. Lipids are digested by lipase enzymes in the GI tract (with the help of bile acids) and are absorbed directly through the cell membrane. Free fatty acids are then resynthesized into triacylglycerols (TAGs) in the enterocytes. Finally, lipid components are repackaged into chylomicrons and transported throughout the body for use or storage.
Lipid metabolism is the synthesis and degradation of lipids in cells, involving the breakdown and storage of fats for energy and the synthesis of structural and functional lipids, such as those involved in the construction of cell membranes. In animals, these fats are obtained from food and are synthesized by the liver. Lipogenesis is the process of synthesizing these fats. The majority of lipids found in the human body from ingesting food are triglycerides and cholesterol.[4] Other types of lipids found in the body are fatty acids and membrane lipids. Lipid metabolism is often considered as the digestion and absorption process of dietary fat; however, there are two sources of fats that organisms can use to obtain energy: from consumed dietary fats and from stored fat.[5] Vertebrates (including humans) use both sources of fat to produce energy for organs such as the heart to function. Since lipids are hydrophobic molecules, they need to be solubilized before their metabolism can begin. Lipid metabolism often begins with hydrolysis, which occurs with the help of various enzymes in the digestive system.Lipid metabolism also occurs in plants, though the processes differ in some ways when compared to animals.[8] The second step after the hydrolysis is the absorption of the fatty acids into the epithelial cells of the intestinal wall.[6] In the epithelial cells, fatty acids are packaged and transported to the rest of the body.[9]
Metabolic processes include lipid digestion, lipid absorption, lipid transportation, lipid storage, lipid catabolism, and lipid biosynthesis. Lipid catabolism is accomplished by a process known as beta oxidation which takes place in the mitochondria and peroxisome cell organelles.
Fatty acid synthesis:- Everything you need to knowGunveenkaur10
Fatty acid synthesis is a very important topic of biotechnology and biochemistry.
The following presentation includes everything from an introduction to fatty acids and de novo synthesis.
At the end are my social media accounts, please consider the following.
Similar to fattyacidsynthesis-120725091829-phpapp01 (1).pdf (20)
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
How to Make a Field invisible in Odoo 17Celine George
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This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
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The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
2. Fatty acids are a class of compounds containing a long
hydrophobic hydrocarbon chain and a terminal carboxylate
group
They exist free in the body as well as fatty acyl esters in more
complex molecules such as triglycerides or phospholipids.
Fatty acids can be oxidized in all tissues, particularly liver and
muscle to provide energy
They are also structural components of membrane lipids such
as phospholipids and glycolipids.
Esterified fatty acids, in the form of triglycerides are stored in
adipose cells
Fatty acids are also precursors of Eicosanoids
3. Diet
Adipolysis
De novo synthesis(from precursors)-
Carbohydrates, protein, and other molecules
obtained from diet in excess of the body’s
need can be converted to fatty acids, which
are stored as triglycerides
4. Fatty acids are synthesized by an extra
mitochondrial system
This system is present in many tissues,
including liver, kidney, brain, lung, mammary
gland, and adipose tissue.
Acetyl-CoA is the immediate substrate, and
free palmitate is the end product.
Its cofactor requirements include NADPH,
ATP, Mn2+, biotin, and HCO3
– (as a source of
CO2).
6. NADPH is involved as donor of reducing
equivalents
The oxidative reactions of the pentose phosphate
pathway are the chief source of the hydrogen
required for the reductive synthesis of fatty acids.
Tissues specializing in active lipogenesis—
ie, liver, adipose tissue, and the lactating
mammary gland—possess an active pentose
phosphate pathway.
Other sources of NADPH include the reaction that
converts malate to pyruvate catalyzed by the "Malic
enzyme" (NADP malate dehydrogenase) and the
extra mitochondrial isocitrate dehydrogenase
reaction (probably not a substantial source, except
7. In hepatocytes, adipose tissue and the lactating
mammary glands, the NADPH is supplied primarily by
the pentose phosphate pathway.
9. There are three isoenzymes of isocitrate
dehydrogenase. One, which uses NAD+, is found
only in mitochondria. The other two use NADP+
and are found in mitochondria and the cytosol.
Respiratory chain-linked oxidation of isocitrate
proceeds almost completely through the NAD+-
dependent enzyme.
10. Acetyl co A, the
precursor for fatty
acid synthesis is
produced from
pyruvate,
ketogenic amino
acids, fatty acid
oxidation and by
alcohol
metabolism
It is a substrate
for TCA cycle and
a precursor for
fatty acids, ketone
bodies and
sterols.
11. Fatty acid synthesis requires considerable
amounts of acetyl-CoA
Nearly all acetyl-CoA used in fatty acid
synthesis is formed in mitochondria
Acetyl co A has to move out from the
mitochondria to the cytosol
Cytosol – site of acetate utilization
Mitochondria – site of acetate
synthesis
12. Acetate is shuttled out of mitochondria as
citrate
The mitochondrial inner membrane is
impermeable to acetyl-CoA
Intra-mitochondrial acetyl-CoA first reacts
with oxaloacetate to form citrate, in the
TCA cycle catalyzed by citrate synthase
Citrate then passes into the cytosol
through the mitochondrial inner membrane
on the citrate transporter.
In the cytosol, citrate is cleaved by citrate
lyase regenerating acetyl-CoA.
13.
14. The other product of Citrate cleavage,
oxaloacetate can be-
Channeled towards glucose production
Converted to malate by malate
dehydrogenase
Converted to Pyruvate by Malic enzyme,
producing more NADPH, that can be used for
fatty acid synthesis
Pyruvate and Malate pass through special
transporters present in the inner
mitochondrial membrane
15.
16. Two main enzymes-
Acetyl co A carboxylase
Fatty acid Synthase
Both the enzymes are multienzyme
complexes
Coenzymes and cofactors are-
Biotin
NADPH
Mn++
Mg++
17. Acetyl co A carboxylase -Is the Initial & Controlling
Step in Fatty Acid Synthesis
Multienzyme complex containing-
Biotin
Biotin Carboxylase
Biotin carboxyl carrier protein
Transcarboxylase
A regulatory allosteric site
18. Fatty acid Synthase complex-
The Fatty Acid Synthase Complex is a
polypeptide containing seven enzyme
activities
In bacteria and plants, the individual
enzymes of the fatty acid synthase system
are separate, and the acyl radicals are found
in combination with a protein called the acyl
carrier protein (ACP).
In yeast, mammals, and birds, the synthase
system is a multienzyme polypeptide
complex that incorporates ACP, which takes
19. In mammals, the fatty acid synthase complex is
a dimer comprising two identical monomers,
each containing all seven enzyme activities of
fatty acid synthase on one polypeptide chain
The use of one multienzyme functional unit has
the advantages of achieving the effect of
compartmentalization of the process within the
cell without the erection of permeability barriers,
Synthesis of all enzymes in the complex is
coordinated since it is encoded by a single gene.
20.
21. The input to fatty acid synthesis is acetyl-CoA, which is
carboxylated to malonyl-CoA. The reaction is catalyzed by Acetyl
co A carboxylase
Step-1
22. ATP-dependent carboxylation provides energy
input.
The CO2 is lost later during condensation with the
growing fatty acid.
The spontaneous decarboxylation drives the
condensation reaction.
As with other carboxylation reactions, the
enzyme prosthetic group is biotin.
The reaction takes place in two steps:
carboxylation of biotin (involving ATP) and transfer
of the carboxyl to acetyl-CoA to form malonyl-
CoA.
23. Biotin is linked to the enzyme by an amide bond
between the terminal carboxyl of the biotin side
chain and the -amino group of a lysine residue.
CH
CH
H2C
S
CH
NH
C
N
O
(CH2)4 C NH (CH2)4 CH
C
O
NH
O
C
O
O
Carboxybiotin lysine
residue
24. The overall reaction, which is spontaneous, may be
summarized as:
HCO3 + ATP + acetyl-CoA ADP + Pi + malonyl-CoA
ll
Enzyme-biotin
HCO3
-
+ ATP
ADP + Pi
Enzyme-biotin-CO2
-
O
CH3-C-SCoA
acetyl-CoA
O
-
O2C-CH2-C-SCoA
malonyl-CoA
ll
Enzyme-biotin
1
2
25. Once malonyl-CoA is synthesized, long
carbon FA chains may be assembled in a
repeating four-step sequence.
With each passage through the cycle the
fatty acyl chain is extended by two carbons.
When the chain reaches 16 carbons, the
product palmitate (16:0) leaves the cycle.
26. All the remaining steps are catalyzed by
Fatty acid synthase complex
Fatty Acid Synthase prosthetic groups:
The thiol (-SH)of the side-chain of a
cysteine residue of keto acyl synthase
enzyme(also called condensing enzyme)
The thiol (-SH)of
phosphopantetheine, equivalent in
structure to part of coenzyme A. It is a
component of Acyl carrier protein
27. Each segment of
the disk represents
one of the six
enzymatic activities
of the complex
(Thioesterase not
shown)
At the center is the
ACP – acyl carrier
protein - with its
phosphopantethein
-e arm ending in –
SH.
28. Phosphopantetheine
(Pant) is covalently
inked via a phosphate
ester to a serine OH of
the acyl carrier protein
domain of Fatty Acid
Synthase.
The long flexible arm
of phosphopantetheine
helps its thiol to move
from one active site to
another within the
complex.
O
P
O
H2C
O
O
C
C
C
NH
CH2
CH2
C
NH
CH3
H3C
H
HO
O
CH2
CH2
SH
O
CH2 CH
NH
C O
-mercaptoethylamine
pantothenate
serine
residue
phosphopantetheine
of acyl carrier protein
phosphate
29. Serve as a flexible
arm, tethering the
growing fatty acyl chain to
the surface of the
synthase complex
Carrying the reaction
intermediates from one
enzyme active site to the
next.
30. To initiate FA biosynthesis, malonyl and acetyl
groups are activated on to the enzyme fatty acid
synthase.
Initially, a priming molecule of acetyl-CoA
combines with a cysteine —SH group catalyzed
by acetyl transacylase
Malonyl-CoA combines with the adjacent —SH
on the 4'-phosphopantetheine of ACP of the
other monomer, catalyzed by malonyl
transacylase (to form acetyl (acyl)-malonyl
enzyme.
31. The acetyl group
from acetyl-CoA is
transferred to the
Cys-SH group of the
-ketoacyl ACP
synthase
This reaction is
catalyzed by acetyl-
CoA transacetylase.
32. Transfer of the
malonyl group to the –
SH group of the ACP is
catalyzed by malonyl-
CoA ACP transferase.
The charged acetyl
and malonyl groups
are now in close
proximity to each
other
33. After activation, the processes involved are-
1. Condensation
2. Reduction
3. Dehydration
4. Reduction
These steps are repeated till a fatty acid with 16
carbon atoms is synthesized
34. The acetyl group attacks the methylene
group of the malonyl residue, catalyzed by
3-ketoacyl synthase, and liberates CO2,
forming 3-ketoacyl enzyme (Acetoacetyl
enzyme),freeing the cysteine —SH group.
Decarboxylation allows the reaction to go to
completion, pulling the whole sequence of
reactions in the forward direction.
35. Condensation –
Condensation of the
activated acetyl and
malonyl groups takes
place to form
Acetoacetyl-ACP
The reaction is
catalyzed by β-
ketoacyl-ACP
synthase.
36. Reduction-
The Acetoacetyl-
ACP is reduced to b-
hydroxybutyryl-
ACP, catalyzed by b-
ketoacyl-ACP
reductase
NADPH + H+ are
required for
reduction
37. Dehydration –
Dehydration yields
a double bond in
the product, trans-
∆2-butenoyl-ACP,
Reaction is
catalyzed by β-
hydroxybutyryl-
ACP dehydratase.
38. Reduction
Reduction of the
double bond takes
place to form butyryl-
ACP,
Reaction is catalyzed
by enoyl-reductase.
Another NADPH
dependent reaction.
39. This reaction
makes way for the
next incoming
malonyl group.
The enzyme
involved is acetyl-
CoA
transacetylase
40. The butyryl group is on
the Cys-SH group
The incoming malonyl
group is first attached to
ACP.
In the condensation
step, the entire butyryl
group is exchanged for
the carboxyl group on the
malonyl residue
41. The 3-ketoacyl group is
reduced, dehydrated, and reduced again
(reactions 2, 3, 4) to form the corresponding
saturated acyl-S-enzyme.
A new malonyl-CoA molecule combines with
the —SH of 4'-phosphopantetheine, displacing
the saturated acyl residue onto the free cysteine
—SH group.
The sequence of reactions are repeated until a
saturated 16-carbon acyl radical (Palmityl) has
been assembled.
It is liberated from the enzyme complex by the
activity of a seventh enzyme in the
complex, Thioesterase (deacylase).
42.
43. Seven cycles of condensation and reduction produce
the 16-carbon saturated palmitoyl group, still bound to
ACP.
Chain elongation usually stops at this point, and free
palmitate is released from the ACP molecule by
hydrolytic activity in the synthase complex.
Smaller amounts of longer fatty acids such as stearate
(18:0) are also formed
In mammary gland, there is a separate Thioesterase
specific for acyl residues of C8, C10, or C12, which are
subsequently found in milk lipids.
44.
45. First, the formation of seven malonyl-CoA molecules:
7Acetyl-CoA + 7CO2 + 7ATP
7malonyl CoA + 7ADP + 7Pi
46. Then the seven cycles of condensation and reduction
Acetyl-CoA + 7malonyl-CoA + 14NADPH + 14H+
palmitate + 7CO2 + 8CoA +
14NADP+ + 6H2O
The biosynthesis of FAs requires acetyl-CoA and the
input of energy in the form of ATP and reducing power
of NADPH.
47. Βeta Oxidation
pathway
Fatty acid Synthesis
Location Mitochondrial Cytoplasmic
Acyl Carriers(Thiols) Coenzyme A 4’
Phosphopantetheine
and Cysteine
Electron acceptors
and donors
FAD/NAD NADPH
OH Intermediates L D
2 Carbon
product/donor
Acetyl co A Acetyl co A/ Malonyl
co A
48. When a cell has
more energy, the
excess is generally
converted to Fatty
Acids and stored as
lipids such as
triacylglycerol.
Glycerol-P
Triacylglycerol
Fatty acyl CoA
Malonyl CoA
Acetyl CoA
Glucose
Pyruvate
TCA cycle
49. The reaction
catalyzed by acetyl-
CoA carboxylase is
the rate limiting
step in the
biosynthesis of
fatty acids.
CH3-C-S-CoA
=
O
HCO3
-
-OOC-CH2-C-S-CoA
=
O
Acetyl-CoA
Malonyl-CoA
50. The mammalian enzyme is regulated, by
Allosteric control by local metabolites
Phosphorylation
Conformational changes associated with
regulation:
In the active conformation, Acetyl-CoA
Carboxylase associates to form multimeric
filamentous complexes.
Transition to the inactive conformation is
associated with dissociation to yield the
monomeric form of the enzyme (protomer).
51. Allosteric control
Palmitoyl-CoA acts as a
feedback inhibitor of the
enzyme, and citrate is an
activator.
When there is an
increase in mitochondrial
acetyl-CoA and
ATP, citrate is
transported out of
mitochondria,
Citrate becomes both
the precursor of cytosolic
acetyl-CoA and a signal
for the activation of
acetyl-CoA carboxylase.
53. Additionally, these pathways are regulated at the
level of gene expression
Long-chain fatty acid synthesis is controlled in
the short term by allosteric and covalent
modification of enzymes and in the long term by
changes in gene expression governing rates of
synthesis of enzymes.
54. Excess carbohydrates is stored as fat in
many animals in anticipation of periods of
caloric deficiency such as
starvation, hibernation, etc, and to provide
energy for use between meals in
animals, including humans, that take their
food at spaced intervals.
The nutritional state of the organism is the
main factor regulating the rate of
lipogenesis.
55. The rate is higher in the well-fed state if the
diet contains a high proportion of
carbohydrate
Lipogenesis converts surplus glucose and
intermediates such as pyruvate, lactate, and
acetyl-CoA to fat, assisting the anabolic
phase of this feeding cycle
Lipogenesis is increased when sucrose is
fed instead of glucose because fructose
bypasses the phosphofructokinase control
point in glycolysis and floods the lipogenic
pathway
56. It is depressed by restricted caloric
intake, high fat diet, or a deficiency of
insulin, as in diabetes mellitus
These conditions are associated with
increased concentrations of plasma free fatty
acids
An inverse relationship has been
demonstrated between hepatic lipogenesis
and the concentration of serum-free fatty
acids.
57. Insulin stimulates lipogenesis by several
other mechanisms as well as by increasing
acetyl-CoA carboxylase activity.
It increases the transport of glucose into the
cell (eg, in adipose tissue),
Increases the availability of both pyruvate
for fatty acid synthesis and glycerol 3-
phosphate for esterification of the newly
formed fatty acids,
58. Insulin converts the inactive form of
pyruvate dehydrogenase to the active form in
adipose tissue but not in liver, thus provides
more of Acetyl co A
Insulin also acts by inhibiting c AMP
mediated lipolysis in adipose tissue and
thereby reduces the concentration of plasma
free fatty acids (long-chain fatty acids are
inhibitors of lipogenesis.
59. Palmitate in animal cells is the precursor
of other long-chained FAs.
By further additions of acetyl groups, fatty
acid chain length is elongated through the
action of FA elongation systems present in
the smooth endoplasmic reticulum and the
mitochondria.
60.
61. Palmitate and stearate serve as precursors
of the two most common monounsaturated
fatty acids of animal cells: palmitoleate
(16:1 9), and Oleate (18:1 9).
The double bond is introduced by fatty
acyl-CoA desaturase in the smooth
endoplasmic reticulum.
62.
63. Mammalian hepatocytes readily introduce
double bonds at the D9 position of FAs but
cannot between C-10 and the methyl-terminal
end.
Linoleate, 18:2D9,12 and linolenate 18:3D9,12,15
cannot be synthesized by mammals, but plants
can synthesize both.
Arachidonic acid is semi essential, since it can
be synthesized from Linoleic acid
64.
65. Most of the FAs synthesized or ingested by
an organism have one of two fates:
Incorporated into triacylglycerols for the
storage of metabolic energy
Incorporated into the phospholipid
components of membranes