this was my report in the uini. that i make for the general uni toi get highest mark i wish u doing same me

1. Faculty of Science
Department of Chemistry,year 2, Semester 2
Experiment no.4
General Biochemistry
)Acrolein,Densten and Cupper test)
Supervisor by
Dr.Araz
Name : Abdullah Muhammad
Date : 4-12-2014
Group : A

2. Introduction of Acrolein
Acrolein is the simplest unsaturated aldehyde. It is a colourless
liquid with a piercing, disagreeable, acrid smell. The smell of
burnt fat (as when cooking oil is heated to its smoke point) is
caused by glycerol in the burning fat breaking down into
acrolein. It is produced industrially from propylene and mainly
used as a biocide and a building block to other chemical
compounds, such as the amino acid methionine.
Introduction of Cupper
cupric acetate, is the chemical compound with
the formula Cu(OAc)2 where OAc- is acetate(CH3CO2-).
The hydrated derivative, which contains one molecule of water
for each Cu atom, is available
commercially. AnhydrousCu(OAc)2 is a dark
green crystalline solid, whereas Cu2(OAc)4(H2O)2 is more
bluish-green. Since ancient times, copper acetates of some
form have been used as fungicides and green pigments. Today,
copper acetates are used as reagents for the synthesis of
various inorganic and organic compounds. Copper acetate, like
all copper compounds, emits a blue-green glow in a flame.
Introductionof Denstan
This test used for detection free glycerol , when free glycerol
added to a mixture of Borax
& Phenol phthalipine causes disappearing the pink color
because glyceroboric acid
formed which are dissociated by heating and pink color
reformed.

3. Aim of Acrolein
To detect presence of fats or glycerin.
Aim of Denstan
To indicates the amount of presence of double bonds in the
lipid sample.
Aim of Cupper
To distinguish between oil or neutral fat and fatty acid
saturated and unsaturated.
Chemicals
First test (Acrolein test)
glycerol-24KHSO-1
Second test (Dnstan)
1-Phynol naphthalene 2-Glycerol
3-Borax
Theird test (cupper)
1-Ether 2-Steric acid
Gllaswares
1-Test tube 2-Beaker
3-Dropper 4-Spatulla

4. Equipments
1-water bath
Method
First test (Acrolein)
(12)and4Take a test tube and putting it a (0.5 gm) of KHSO-1
drop of glycerol transfer a test tube to a water bath .
Second test (Denstan)
(1ml) of phynol naphthalene into a test tube after that 5 drop
of grycerol is added then (2) drop of borax is added .
Theird test (Cupper)
Take two test tubes put(1 / 2) g of each sample and then added
20 drop of petroleum ether and an equal volume of a solution
of copper acetate .
Result
in the first test I was finished the test by boiling and malodor.
Second test I was finished the test by change the color to pink.
Theird test I was finished the test by formed two layer the up
layer is petroleum ether and the botton layer is cupper acetate.

5. Conclusion
In the first test the result of the test was malodor because I
.4heated a grycerol and added catalyst KHSO
In the second test These unsaturated fatty acids become
saturated by taking up a cupper acetate.
If the fat contains more unsaturated fatty acids, it will take up
more copper acetate.
Theird test that the petroleum ether upper layer remains
colorless, while consists of pale green precipitate of
copper stearate at the bottom.
Reference
http://en.wikipedia.org/wiki/Acrolein
http://fac.ksu.edu.sa/sites/default/files/Qualitative%20test%20of%20Lipids%20II.pdf