Fabrication of suspended gel membranes on paper and plastic substrates

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(a) Paper-based μPADs: A wax pattern is printed on chromatography paper and melted on a hot plate to create a hydrophobic barrier [(i) and (ii)]. A circular pattern is excised using a biopsy punch to create a cut hole (iii). The paper is lowered onto a 100 μl droplet of 2% liquid agar and gently lifted, resulting in an agar membrane in the cut hole [(iv) and (v)]. Besides agar, this droplet method also works for Pluronic gel. (b) Plastic-based μPADs: A plastic sheet is taped on both sides, and multiple circular patterns are excised using an automated design cutter to create cut holes [(i) and (ii)]. The excised plastic sheet is vertically immersed into an 11.5% solution of Pluronic gel and removed, resulting in suspended membranes of Pluronic gel within the cut holes [iii) and (iv)].

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FIG. 7. Fabrication of suspended gel membranes on paper and plastic substrates. (a) Paper-based μPADs: A wax pattern is printed on chromatography paper and melted on a hot plate to
create a hydrophobic barrier [(i) and (ii)]. A circular pattern is excised using a biopsy punch to create a cut hole (iii). The paper is lowered onto a 100 μl droplet of 2% liquid agar and gently
lifted, resulting in an agar membrane in the cut hole [(iv) and (v)]. Besides agar, this droplet method also works for Pluronic gel. (b) Plastic-based μPADs: A plastic sheet is taped on both sides,
and multiple circular patterns are excised using an automated design cutter to create cut holes [(i) and (ii)]. The excised plastic sheet is vertically immersed into an 11.5% solution of Pluronic
gel and removed, resulting in suspended membranes of Pluronic gel within the cut holes [iii) and (iv)].
Published in: Zach Njus; Taejoon Kong; Upender Kalwa; Christopher Legner; Matthew Weinstein; Shawn Flanigan; Jenifer Saldanha; Santosh Pandey;
APL Bioengineering 1, 016102 (2017)
DOI: 10.1063/1.5005829
Copyright © 2017 Author(s)

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Fabrication of suspended gel membranes on paper and plastic substrates

1. FIG. 7. Fabrication of suspended gel membranes on paper and plastic substrates. (a) Paper-based μPADs: A wax pattern is printed on chromatography paper and melted on a hot plate to create a hydrophobic barrier [(i) and (ii)]. A circular pattern is excised using a biopsy punch to create a cut hole (iii). The paper is lowered onto a 100 μl droplet of 2% liquid agar and gently lifted, resulting in an agar membrane in the cut hole [(iv) and (v)]. Besides agar, this droplet method also works for Pluronic gel. (b) Plastic-based μPADs: A plastic sheet is taped on both sides, and multiple circular patterns are excised using an automated design cutter to create cut holes [(i) and (ii)]. The excised plastic sheet is vertically immersed into an 11.5% solution of Pluronic gel and removed, resulting in suspended membranes of Pluronic gel within the cut holes [iii) and (iv)]. Published in: Zach Njus; Taejoon Kong; Upender Kalwa; Christopher Legner; Matthew Weinstein; Shawn Flanigan; Jenifer Saldanha; Santosh Pandey; APL Bioengineering 1, 016102 (2017) DOI: 10.1063/1.5005829 Copyright © 2017 Author(s)

Fabrication of suspended gel membranes on paper and plastic substrates

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Fabrication of suspended gel membranes on paper and plastic substrates