F+ cells have fertility factors while F- cells lack fertility factors F+ is a donor cells while F- is recipient cells F+ cells has circular plasmid separate from chromosome F- cell does not have before receiving plasmid Cell connect through T-pilus Tra K and tra I were required for efficient inter plasmid recombination at ori T whole DNA is transferred to recipient F- Solution F+ cells have fertility factors while F- cells lack fertility factors F+ is a donor cells while F- is recipient cells F+ cells has circular plasmid separate from chromosome F- cell does not have before receiving plasmid Cell connect through T-pilus Tra K and tra I were required for efficient inter plasmid recombination at ori T whole DNA is transferred to recipient F-.